Titre : |
Investigation phytochimique et recherche d’activité biologique de deux espèces du genre Centaurea (Asteraceae) |
Type de document : |
texte imprimé |
Auteurs : |
Djihane Azzouzi, Auteur ; Ratiba Mekkiou, Directeur de thèse |
Editeur : |
جامعة الإخوة منتوري قسنطينة |
Année de publication : |
2017 |
Importance : |
227 f. |
Format : |
30 cm. |
Note générale : |
Doctorat 3éme CYCLE.
2 copies imprimées disponibles
|
Langues : |
Français (fre) |
Catégories : |
Français - Anglais Chimie
|
Tags : |
Chimie pharmaceutique:Analyse physicochimique, contrôle de la qualité et synthèse de substances bioactives Centaurea choulettiana Centaurea fragilis HPLC-TOF/MS GC-MS acides
phénoliques flavonoïdes activité antioxydante activité anticancéreuse huiles essentielles Centaurea choulettiana Pomel phenolic acids flavonoïds antioxidant activity antiproliferative activity essential oil أحماض فينولية الفلافويويدات الزيوت
الطيارة النشاطية المضادة للميكروبات النشاطية المضادة للأكسدة النشاطية المضادة للسرطان |
Index. décimale : |
540 Chimie et sciences connexes |
Résumé : |
This work is devoted to the phytochemical and biological study of two species of the genus Centaurea (Asteraceae), endemic to Algeria and has never been studied before. They are Centaurea choulettiana Pomel and Centaurea fragilis Dur.
The phytochemical investigation of cyclohexane, ethyl acetate and n-butanol extracts from the aerial parts of C. choulettiana and n-butanol extract of C. fragilis allowed the isolation and identification of 13 products. The structural elucidation of the isolated compounds was carried out by combining the various spectroscopic methods (UV, 1D & 2D NMR, HPLC/TOF-MS and GC/MS) as well as by comparing the results with the data of the literature.
The analysis of essential oil (flowers) of C. choulettiana by the GC/MS technique, carried out for the first time, showed the presence of several chemical groups from which, phthalate (18.38%) followed by β-eudesmol (6.8%), 5-epoxysalvial-4(14)-ene (6.6%), caryophyllene oxide (4.28%), tricosane (3.31%), 7-epi-α-eudesmol (3.08%) and heptacosane. The antibacterial activity of this oil was determined against five bacterial strains according to the disk diffusion method, the results showed modest activity against all the strains.
Quantification of the total polyphenols and total flavonoids of the EtOAc and n-BuOH (leaves and flowers) extracts of C. choulettiana was performed by colorimetric methods. This step showed a content of polyphenols and flavonoids more important in the AcOEt extract of the leaves. In order to identify and quantify the phenolic acids present in these extracts, chemical screening was performed by HPLC-TOF/MS, this revealed the presence of 9 phenolic acids in various amounts. The HPLC-TOF / MS analysis of the n-BuOH (leaves) extract of C. fragilis revealed the presence of 9 phenolic acids and 10 flavonoïds.
The antioxidant activity of the EtOAc and n-BuOH (leaves and flowers) extracts of C.
choulettiana was evaluated by the free radical scavenging method (DPPH) and the inhibition of lipid peroxidation (LPO). The EtOAc (leaves) extract exhibited the strongest effect with respect to the DPPH free radical (96.54%) and the inhibition of lipid peroxidation (64.17%).
Concerning the antiproliferative activity, the results showed that the cyclohexane extract of C.
choulettiana was the most active against HeLa cells (carcinoma of the human cervix), while the n-BuOH extract of C. fragilis was inactive in his unlevel doses.
|
Note de contenu : |
Article1: Essential Oil Composition of Centaurea choulettiana Pomel(Asteraceae) from Algeria.
Djihane Azzouzi1, Ratiba Mekkiou1*, Pierre Chalard2, Jean-Claude Chalchat2,Ouahiba Boumaza1, Ramdane Seghiri1, Fadila Benayache1, Samir Benayache.
- Article:Qualitative and Quantitative Analysis of Polar Extract fromCentaurea fragilisDur. Using HPLC-TOF/MS-Phenolic Profile and Antioxidant Activity ofCentaurea choulettiana Pomel (Asteraceae) Extracts.
Djihane Azzouzi1, Kenza Bioud2, Ibrahim Demirtas3, Fatih Gül3, Djamel Sarri4, Samir Benayache1Fadila Benayache1and Ratiba Mekkiou1,
-Article:Phenolic Profile and Antioxidant Activity ofCentaurea choulettiana Pomel (Asteraceae) Extracts.
Djihane Azzouzi1, Kenza Bioud2, Ibrahim Demirtas3, Fatih Gül3, Djamel Sarri4, Samir Benayache1Fadila Benayache1and Ratiba Mekkiou1,*
|
Diplôme : |
Doctorat |
En ligne : |
../theses/chimie/AZZ6992.pdf |
Format de la ressource électronique : |
pdf |
Permalink : |
index.php?lvl=notice_display&id=10402 |
Investigation phytochimique et recherche d’activité biologique de deux espèces du genre Centaurea (Asteraceae) [texte imprimé] / Djihane Azzouzi, Auteur ; Ratiba Mekkiou, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 227 f. ; 30 cm. Doctorat 3éme CYCLE.
2 copies imprimées disponibles
Langues : Français ( fre)
Catégories : |
Français - Anglais Chimie
|
Tags : |
Chimie pharmaceutique:Analyse physicochimique, contrôle de la qualité et synthèse de substances bioactives Centaurea choulettiana Centaurea fragilis HPLC-TOF/MS GC-MS acides
phénoliques flavonoïdes activité antioxydante activité anticancéreuse huiles essentielles Centaurea choulettiana Pomel phenolic acids flavonoïds antioxidant activity antiproliferative activity essential oil أحماض فينولية الفلافويويدات الزيوت
الطيارة النشاطية المضادة للميكروبات النشاطية المضادة للأكسدة النشاطية المضادة للسرطان |
Index. décimale : |
540 Chimie et sciences connexes |
Résumé : |
This work is devoted to the phytochemical and biological study of two species of the genus Centaurea (Asteraceae), endemic to Algeria and has never been studied before. They are Centaurea choulettiana Pomel and Centaurea fragilis Dur.
The phytochemical investigation of cyclohexane, ethyl acetate and n-butanol extracts from the aerial parts of C. choulettiana and n-butanol extract of C. fragilis allowed the isolation and identification of 13 products. The structural elucidation of the isolated compounds was carried out by combining the various spectroscopic methods (UV, 1D & 2D NMR, HPLC/TOF-MS and GC/MS) as well as by comparing the results with the data of the literature.
The analysis of essential oil (flowers) of C. choulettiana by the GC/MS technique, carried out for the first time, showed the presence of several chemical groups from which, phthalate (18.38%) followed by β-eudesmol (6.8%), 5-epoxysalvial-4(14)-ene (6.6%), caryophyllene oxide (4.28%), tricosane (3.31%), 7-epi-α-eudesmol (3.08%) and heptacosane. The antibacterial activity of this oil was determined against five bacterial strains according to the disk diffusion method, the results showed modest activity against all the strains.
Quantification of the total polyphenols and total flavonoids of the EtOAc and n-BuOH (leaves and flowers) extracts of C. choulettiana was performed by colorimetric methods. This step showed a content of polyphenols and flavonoids more important in the AcOEt extract of the leaves. In order to identify and quantify the phenolic acids present in these extracts, chemical screening was performed by HPLC-TOF/MS, this revealed the presence of 9 phenolic acids in various amounts. The HPLC-TOF / MS analysis of the n-BuOH (leaves) extract of C. fragilis revealed the presence of 9 phenolic acids and 10 flavonoïds.
The antioxidant activity of the EtOAc and n-BuOH (leaves and flowers) extracts of C.
choulettiana was evaluated by the free radical scavenging method (DPPH) and the inhibition of lipid peroxidation (LPO). The EtOAc (leaves) extract exhibited the strongest effect with respect to the DPPH free radical (96.54%) and the inhibition of lipid peroxidation (64.17%).
Concerning the antiproliferative activity, the results showed that the cyclohexane extract of C.
choulettiana was the most active against HeLa cells (carcinoma of the human cervix), while the n-BuOH extract of C. fragilis was inactive in his unlevel doses.
|
Note de contenu : |
Article1: Essential Oil Composition of Centaurea choulettiana Pomel(Asteraceae) from Algeria.
Djihane Azzouzi1, Ratiba Mekkiou1*, Pierre Chalard2, Jean-Claude Chalchat2,Ouahiba Boumaza1, Ramdane Seghiri1, Fadila Benayache1, Samir Benayache.
- Article:Qualitative and Quantitative Analysis of Polar Extract fromCentaurea fragilisDur. Using HPLC-TOF/MS-Phenolic Profile and Antioxidant Activity ofCentaurea choulettiana Pomel (Asteraceae) Extracts.
Djihane Azzouzi1, Kenza Bioud2, Ibrahim Demirtas3, Fatih Gül3, Djamel Sarri4, Samir Benayache1Fadila Benayache1and Ratiba Mekkiou1,
-Article:Phenolic Profile and Antioxidant Activity ofCentaurea choulettiana Pomel (Asteraceae) Extracts.
Djihane Azzouzi1, Kenza Bioud2, Ibrahim Demirtas3, Fatih Gül3, Djamel Sarri4, Samir Benayache1Fadila Benayache1and Ratiba Mekkiou1,*
|
Diplôme : |
Doctorat |
En ligne : |
../theses/chimie/AZZ6992.pdf |
Format de la ressource électronique : |
pdf |
Permalink : |
index.php?lvl=notice_display&id=10402 |
|