| Titre : |
Secondary metabolic components and biological effectiveness study on two species Thymelaea microphyllaand Gnidia somalensis |
| Type de document : |
texte imprimé |
| Auteurs : |
Ali Saeed Noman Labib, Auteur ; Amar Zellagui, Directeur de thèse ; Salah Rhouati, Directeur de thèse |
| Editeur : |
جامعة الإخوة منتوري قسنطينة |
| Année de publication : |
2017 |
| Importance : |
234 f. |
| Format : |
30 cm. |
| Note générale : |
2 copies imprimées disponibles
|
| Langues : |
Anglais (eng) |
| Catégories : |
Français - Anglais
Chimie
|
| Tags : |
Thymelaea microphylla antioxidant activity antimicrobialactivity microphybenzimidazol antioxidant activité antimicrobialactivités الفعالية المضادة للأكسد الفعالية المضادة للميكروبات |
| Index. décimale : |
540 Chimie et sciences connexes |
| Résumé : |
The main objective of our work is phytochemical studies and evaluation
of secondary metabolite products extracted from Thymelaeaceae family
which is rich in biological active phenolic compounds. So, we choose
Thymelaea microphylla, which grows in Algeria. In addition to another plant
from the same family; Gnidia somalensis growing in Yemen.
Our study also, devoted to the evaluation and effectiveness of the plant
extracts and pure compounds against cancer cells. We used the ELISA and
xCELLigence assay technics for determining the inhibition of fractions and
pure compounds in vitro, against HeLa cells (human uterus carcinoma) and
C6 cells (rat brain tumor). Which have shown positive results, especially for
fractions that have a high activity compared with the reference compound. In
addition, we have got a significant results by testing the pure compounds
(Prestegane B, daphnoretin and trans-tiliroside) which gave a positive results.
On the other hand, we were able to isolated 10 compounds from T.
microphylla:
• Two lignan lactones.
• One bicoumarin.
• Four flavonoids.
• Two spiro lactones.
• One new alkaloid (microphybenzimidazole).
Also, we have separated 12 phenolic compounds from the plant Gnidia
somalensis: 6 polysubstituted cinnamic acid derivatives and 6 flavonoids.
We used different chromatographic methods for separation and
purification (Column chromatography CC, thin layer chromatography TLC),
and spectroscopic tools for structure identification (HPLC LC-MS, HPLCTOF), ID (Tf -NMR, 13C- NMR and DEPT) and 2D (HMQC, HMBC and
COSY).
|
| Diplôme : |
Doctorat en sciences |
| En ligne : |
../theses/chimie/LAB7152.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=10431 |
Secondary metabolic components and biological effectiveness study on two species Thymelaea microphyllaand Gnidia somalensis [texte imprimé] / Ali Saeed Noman Labib, Auteur ; Amar Zellagui, Directeur de thèse ; Salah Rhouati, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 234 f. ; 30 cm. 2 copies imprimées disponibles
Langues : Anglais ( eng)
| Catégories : |
Français - Anglais
Chimie
|
| Tags : |
Thymelaea microphylla antioxidant activity antimicrobialactivity microphybenzimidazol antioxidant activité antimicrobialactivités الفعالية المضادة للأكسد الفعالية المضادة للميكروبات |
| Index. décimale : |
540 Chimie et sciences connexes |
| Résumé : |
The main objective of our work is phytochemical studies and evaluation
of secondary metabolite products extracted from Thymelaeaceae family
which is rich in biological active phenolic compounds. So, we choose
Thymelaea microphylla, which grows in Algeria. In addition to another plant
from the same family; Gnidia somalensis growing in Yemen.
Our study also, devoted to the evaluation and effectiveness of the plant
extracts and pure compounds against cancer cells. We used the ELISA and
xCELLigence assay technics for determining the inhibition of fractions and
pure compounds in vitro, against HeLa cells (human uterus carcinoma) and
C6 cells (rat brain tumor). Which have shown positive results, especially for
fractions that have a high activity compared with the reference compound. In
addition, we have got a significant results by testing the pure compounds
(Prestegane B, daphnoretin and trans-tiliroside) which gave a positive results.
On the other hand, we were able to isolated 10 compounds from T.
microphylla:
• Two lignan lactones.
• One bicoumarin.
• Four flavonoids.
• Two spiro lactones.
• One new alkaloid (microphybenzimidazole).
Also, we have separated 12 phenolic compounds from the plant Gnidia
somalensis: 6 polysubstituted cinnamic acid derivatives and 6 flavonoids.
We used different chromatographic methods for separation and
purification (Column chromatography CC, thin layer chromatography TLC),
and spectroscopic tools for structure identification (HPLC LC-MS, HPLCTOF), ID (Tf -NMR, 13C- NMR and DEPT) and 2D (HMQC, HMBC and
COSY).
|
| Diplôme : |
Doctorat en sciences |
| En ligne : |
../theses/chimie/LAB7152.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=10431 |
|
Secondary metabolic components and biological effectiveness study on two species Thymelaea microphyllaand Gnidia somalensis
