| Titre : |
Etude de la Laccase produite par des Mycètes isolés à partir de différents ecosystèmes Algériens |
| Type de document : |
texte imprimé |
| Auteurs : |
Sara Benhassine, Auteur ; Noreddine Kacem Chaouche, Directeur de thèse |
| Editeur : |
جامعة الإخوة منتوري قسنطينة |
| Année de publication : |
2017 |
| Importance : |
104 f. |
| Format : |
30 cm. |
| Note générale : |
2 copies imprimées disponibles
|
| Langues : |
Français (fre) |
| Catégories : |
Français - Anglais
Biologie
|
| Tags : |
لكاز بقايا اليقطين أخضر الملكيت أحمر الكونغو
Laccase Trichoderma longibrachiatum Trametes versicolor Chaetomium
globosum pumpkin waste Malachite Green Congo Red déchets de citrouille Vert de Malachite Rouge Congo |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
1 fungal isolates were isolated from forests in eastern Algeria (Kenouaâ, Belezma and
Chettaba), three of them showed positive laccase activity. The morphological and molecular
study carried out allowed identifying them as Trichoderma longibrachiatum, Trametes
versicolor and Chaetomium globosum.
The production kinetics of laccase on PDB medium exposed that Chaetomium globosum
produced a large amount of laccase after 12 days of fermentation, reaching 550 U / L in
Erlens and 987U / L in 20 L fermenter, while Trametes versicolor secretes after 8 days of
fermentation 1400 U / L of laccasic activity in Erlens and 833U / L in fermenter (20L).
Furthermore, the laccase activity secreted by Trametes versicolor has been shown to be at its
maximum at pH 3 and at 55 ° C., whereas the enzymatic activity produced by Chaetomium
globosum reaches its maximum at 35 ° C. and at pH between 5 and 6.
The partial characterization of laccase performed on a Native page gel revealed two different
isozymes produced by Trametes versicolor (Lac1 and Lac2) and a single isozyme produced
by Chaetomium globosum.
The study of laccase production using waste media showed the ability of Trametes versicolor
and Chaetomium globosum to produce laccase on all media tested, however, the optimum of
laccase produced by Trametes versicolor (350U / L) and by Chaetomium globosum (400 U /
L) is obtained on pumpkin waste medium. According to the literature consulted, this work
is the first to use these two fungal strains for the production of laccase using only the
pumpkin waste as the exclusive source of carbon.
The study of synthetic dyes degradation carried out on solid medium and on submerged
medium, demonstrated that selective strains degrade two dyes effectively: Malachite Green
and Congo Red. Degradation was followed by spectrophotometric analysis and HPLC. It was
found that 100% decolorization of Congo Red by Trametes versicolor’s laccase was complete
after 45 minutes of reaction and Malachite green after 60 minutes. On the other hand, the
laccasic extract from Chaetomium globosum allowed a 100% discoloration of Malachite
Green and Congo Red after 55 minutes of reaction. This result can be promising, and
considerable progress in the field of biotechnological applications.
The literature consulted did not mention the ability of Chaetomium species to degrade
Malachite Green or Congo Red. As a result, this work is considered original. |
| Diplôme : |
Doctorat |
| En ligne : |
../theses/biologie/BEN7062.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10539 |
Etude de la Laccase produite par des Mycètes isolés à partir de différents ecosystèmes Algériens [texte imprimé] / Sara Benhassine, Auteur ; Noreddine Kacem Chaouche, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 104 f. ; 30 cm. 2 copies imprimées disponibles
Langues : Français ( fre)
| Catégories : |
Français - Anglais
Biologie
|
| Tags : |
لكاز بقايا اليقطين أخضر الملكيت أحمر الكونغو
Laccase Trichoderma longibrachiatum Trametes versicolor Chaetomium
globosum pumpkin waste Malachite Green Congo Red déchets de citrouille Vert de Malachite Rouge Congo |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
1 fungal isolates were isolated from forests in eastern Algeria (Kenouaâ, Belezma and
Chettaba), three of them showed positive laccase activity. The morphological and molecular
study carried out allowed identifying them as Trichoderma longibrachiatum, Trametes
versicolor and Chaetomium globosum.
The production kinetics of laccase on PDB medium exposed that Chaetomium globosum
produced a large amount of laccase after 12 days of fermentation, reaching 550 U / L in
Erlens and 987U / L in 20 L fermenter, while Trametes versicolor secretes after 8 days of
fermentation 1400 U / L of laccasic activity in Erlens and 833U / L in fermenter (20L).
Furthermore, the laccase activity secreted by Trametes versicolor has been shown to be at its
maximum at pH 3 and at 55 ° C., whereas the enzymatic activity produced by Chaetomium
globosum reaches its maximum at 35 ° C. and at pH between 5 and 6.
The partial characterization of laccase performed on a Native page gel revealed two different
isozymes produced by Trametes versicolor (Lac1 and Lac2) and a single isozyme produced
by Chaetomium globosum.
The study of laccase production using waste media showed the ability of Trametes versicolor
and Chaetomium globosum to produce laccase on all media tested, however, the optimum of
laccase produced by Trametes versicolor (350U / L) and by Chaetomium globosum (400 U /
L) is obtained on pumpkin waste medium. According to the literature consulted, this work
is the first to use these two fungal strains for the production of laccase using only the
pumpkin waste as the exclusive source of carbon.
The study of synthetic dyes degradation carried out on solid medium and on submerged
medium, demonstrated that selective strains degrade two dyes effectively: Malachite Green
and Congo Red. Degradation was followed by spectrophotometric analysis and HPLC. It was
found that 100% decolorization of Congo Red by Trametes versicolor’s laccase was complete
after 45 minutes of reaction and Malachite green after 60 minutes. On the other hand, the
laccasic extract from Chaetomium globosum allowed a 100% discoloration of Malachite
Green and Congo Red after 55 minutes of reaction. This result can be promising, and
considerable progress in the field of biotechnological applications.
The literature consulted did not mention the ability of Chaetomium species to degrade
Malachite Green or Congo Red. As a result, this work is considered original. |
| Diplôme : |
Doctorat |
| En ligne : |
../theses/biologie/BEN7062.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10539 |
|
Etude de la Laccase produite par des Mycètes isolés à partir de différents ecosystèmes Algériens
