Titre : |
Etude de la biodégradation de l’ortho-nitrophénol et du para-nitrophénol par le microbiote des eaux usées et mise en évidence des bactéries dominantes impliquées dans ce processus |
Type de document : |
texte imprimé |
Auteurs : |
Amel Daffri, Auteur ; Ilhem Mihoubi-Djezzare, Directeur de thèse |
Editeur : |
جامعة الإخوة منتوري قسنطينة |
Année de publication : |
2017 |
Importance : |
116 f. |
Format : |
30 cm. |
Note générale : |
2 copies imprimées disponibles.
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Langues : |
Français (fre) |
Catégories : |
Français - Anglais Biologie
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Tags : |
Biodégradation PNP ONP Microbiote Eaux usées Pseudomonas aeruginosa Bacillus cereus Biodegradation Microflora Wastewater نيتورفينول -pنيتروفينول الھدم الحيوي ميكروفلورا المياه المستعملة |
Index. décimale : |
570 Sciences de la vie. Biologie |
Résumé : |
Biodegradation of ortho-Nitrophenol (ONP) and para-Nitrophenol (PNP) by wastewater’s microflora is studied. Isolation and identification of microorganisms implicated is also carried out. The cultures are performed using a minimum medium, where Nitrophenol is the sole source of carbon, energy and nitrogen at 500 mg/L. The kinetic of biodegradation is followed for more than 30 days of incubation. The obtained results show that more than
90% of initial concentration of PNP is decomposed at the end of 30 days of incubation and 77% of ONP in the same time of incubation. HPLC and GC-MS analysis permit to identify products and pathway's biodegradation of the two isomers. The isolation of microorganisms degrading PNP gave four bacterial colonies of different macroscopic aspects. However, three types of these bacterial are pigmented. Sequence analysis of 16S ribosomal RNA indicated
that the ONP and the PNP degrading isolates were closely related to members of the species: Pseudomonas aeruginosa and Bacillus cereus.
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Diplôme : |
Doctorat en sciences |
En ligne : |
../theses/biologie/DAF7170.pdf |
Format de la ressource électronique : |
pdf |
Permalink : |
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