| Titre : |
Le rôle de Thymelaea microphylla dans l’amélioration du stress oxydant et du taux de la métallothionéine induits par le cadmium dans le rein chez le rat. |
| Type de document : |
texte imprimé |
| Auteurs : |
Samira Laib, Auteur ; Korichi Lalaoui, Directeur de thèse |
| Editeur : |
جامعة الإخوة منتوري قسنطينة |
| Année de publication : |
2018 |
| Importance : |
94 f. |
| Format : |
30 cm. |
| Note générale : |
2 copies imprimées disponibles
|
| Langues : |
Français (fre) |
| Catégories : |
Français - Anglais
Biologie
|
| Tags : |
chlorure de cadmium rein stress oxydatif Thymelaea microphylla métallothionéine cadmium chloride kidney oxidative stress metallothionein لوريد الكادميوم الكلية والإجهاد التأكسدي Thymelaea microphyllaميتالوثيونيين |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
The present investigation was performed to evaluate the protective effect and the antioxidant power of Thymelaea microphylla against cadmium chloride induced oxidative stress and renal toxicity in Wistar rats. First, antioxidant and radical scavenging effect of Thymelaea microphylla was evaluated in vitro with 2,2- diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radicals assays. Then, Wistar rats were subjected to oral pretreatment with n-butanolic
extract of Thymelaea microphylla (BETM) (200 mg/kg) against the renal toxicity induced by oral administration of cadmium chloride CdCl2 (5 mg/kg) for 28 days, vitamin E (250 mg/Kg) was used similarly as standard. Efficiency of Thymelaea microphylla against the renal toxicity was evaluated in terms of biochemical estimation of renal function parameters, antioxidant enzymes activities, metallothionein levels and histopathological modifications. Thymelaea
microphylla anti-inflammatory effect was evaluated by carrageenan (1%) induced rat paw edema. Thymelaea microphylla revealed a high scavenging ability of DPPH and OH radicals.
Also, Thymelaea microphylla (200 mg/Kg) pretreatment prevented deteriorative effects caused by cadmium chloride through a protective mechanism that included significant decrease in the levels of Blood Urea Nitrogen, creatinine, gamma-glutamyltranspeptidase (GGT), potassium,sodium, Myeloperoxidase, Nitric Oxide and Thiobarbituric Acid Reactive Species as well as by restoration of antioxidant enzymes in the cytosol, metallothionein levels and
histopathological changes induced by cadmium. Additionally, Thymelaea microphylla revealed an important anti-inflammatory effect reducing carrageenan induced paw edema. This study proved the antioxidant and protective effect of Thymelaea microphylla against cadmium chloride induced oxidative stress and kidney injury.
|
| Diplôme : |
Doctorat |
| En ligne : |
../theses/biologie/LAI7250.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=10842 |
Le rôle de Thymelaea microphylla dans l’amélioration du stress oxydant et du taux de la métallothionéine induits par le cadmium dans le rein chez le rat. [texte imprimé] / Samira Laib, Auteur ; Korichi Lalaoui, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 94 f. ; 30 cm. 2 copies imprimées disponibles
Langues : Français ( fre)
| Catégories : |
Français - Anglais
Biologie
|
| Tags : |
chlorure de cadmium rein stress oxydatif Thymelaea microphylla métallothionéine cadmium chloride kidney oxidative stress metallothionein لوريد الكادميوم الكلية والإجهاد التأكسدي Thymelaea microphyllaميتالوثيونيين |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
The present investigation was performed to evaluate the protective effect and the antioxidant power of Thymelaea microphylla against cadmium chloride induced oxidative stress and renal toxicity in Wistar rats. First, antioxidant and radical scavenging effect of Thymelaea microphylla was evaluated in vitro with 2,2- diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radicals assays. Then, Wistar rats were subjected to oral pretreatment with n-butanolic
extract of Thymelaea microphylla (BETM) (200 mg/kg) against the renal toxicity induced by oral administration of cadmium chloride CdCl2 (5 mg/kg) for 28 days, vitamin E (250 mg/Kg) was used similarly as standard. Efficiency of Thymelaea microphylla against the renal toxicity was evaluated in terms of biochemical estimation of renal function parameters, antioxidant enzymes activities, metallothionein levels and histopathological modifications. Thymelaea
microphylla anti-inflammatory effect was evaluated by carrageenan (1%) induced rat paw edema. Thymelaea microphylla revealed a high scavenging ability of DPPH and OH radicals.
Also, Thymelaea microphylla (200 mg/Kg) pretreatment prevented deteriorative effects caused by cadmium chloride through a protective mechanism that included significant decrease in the levels of Blood Urea Nitrogen, creatinine, gamma-glutamyltranspeptidase (GGT), potassium,sodium, Myeloperoxidase, Nitric Oxide and Thiobarbituric Acid Reactive Species as well as by restoration of antioxidant enzymes in the cytosol, metallothionein levels and
histopathological changes induced by cadmium. Additionally, Thymelaea microphylla revealed an important anti-inflammatory effect reducing carrageenan induced paw edema. This study proved the antioxidant and protective effect of Thymelaea microphylla against cadmium chloride induced oxidative stress and kidney injury.
|
| Diplôme : |
Doctorat |
| En ligne : |
../theses/biologie/LAI7250.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=10842 |
|
Le rôle de Thymelaea microphylla dans l’amélioration du stress oxydant et du taux de la métallothionéine induits par le cadmium dans le rein chez le rat.
