| Titre : |
Apport des techniques moléculaires et bioinformatiques pour l'identification et la caractérisation fonctionnelle de germes causant les mammites subcliniques et les infections respiratoires chez les bovins à Constantine et à Batna. |
| Type de document : |
texte imprimé |
| Auteurs : |
Assia Guendouze, Auteur ; Mohamed Abdelhafid Hamidechi, Directeur de thèse |
| Editeur : |
جامعة الإخوة منتوري قسنطينة |
| Année de publication : |
2018 |
| Importance : |
164 f. |
| Format : |
30 cm. |
| Note générale : |
2 copies imprimées disponibles
|
| Langues : |
Français (fre) |
| Catégories : |
Français - Anglais
Biologie
|
| Tags : |
Mammites subcliniques bovine MLADI-TOF MS quorum quenching lactonase Cattle Subclinical mastitis التهاب ضرع البقر تحت السريري MALDI-TOF MSإدراك الن صاب إنزيم اللاكتوناز |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
The aim of this study is, firstly, to evaluate the method of MALDI-TOF MS for bacteriological identification in the case of subclinical mastitis in dairy cattle and secondly, to test a new antivirulent strategy against a bacterial species P. aeruginosa causing respiratory infections in calves.
The first part consists in identifying 14 bacteria responsible for subclinical mastitis by MALDI-TOF MS confronted with the 16S rDNA and the housekeeping gene rpoB sequencing technique. Our results showed high reliability of identification by MALDI-TOF MS at the species level covering 92.8% of cases (13 isolates) with a score greater than 2. MALDI-TOF MS and bioinformatic analysis are tools that can substitute the identification by simple sequencing using the 16S and rpoB rDNA genes.
The second part consists of evaluating the efficacy of the enzyme SsoPox-W263I, to prevent the establishment of virulence in three strains of Pseudomonas aeruginosa isolated from pneumonic calves, the model strain P. aeruginosa PAO1 and three human strains. For this, we evaluated in vitro the amount of pyocyanin, the proteolytic activity and the biofilm. In the presence of SsoPox W263I, we observed a decrease in proteolytic activity and the production of pyocyanin in 100% of the isolates. Biofilm synthesis decreased in 86% of isolates. The enzyme SsoPox W263I by
inhibition of quorum sensing attenuates the pathogenic power of P. aeruginosa. It can therefore be a promising alternative to conventional treatments.
|
| Diplôme : |
Doctorat |
| En ligne : |
../theses/biologie/GUE7255.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=10847 |
Apport des techniques moléculaires et bioinformatiques pour l'identification et la caractérisation fonctionnelle de germes causant les mammites subcliniques et les infections respiratoires chez les bovins à Constantine et à Batna. [texte imprimé] / Assia Guendouze, Auteur ; Mohamed Abdelhafid Hamidechi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 164 f. ; 30 cm. 2 copies imprimées disponibles
Langues : Français ( fre)
| Catégories : |
Français - Anglais
Biologie
|
| Tags : |
Mammites subcliniques bovine MLADI-TOF MS quorum quenching lactonase Cattle Subclinical mastitis التهاب ضرع البقر تحت السريري MALDI-TOF MSإدراك الن صاب إنزيم اللاكتوناز |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
The aim of this study is, firstly, to evaluate the method of MALDI-TOF MS for bacteriological identification in the case of subclinical mastitis in dairy cattle and secondly, to test a new antivirulent strategy against a bacterial species P. aeruginosa causing respiratory infections in calves.
The first part consists in identifying 14 bacteria responsible for subclinical mastitis by MALDI-TOF MS confronted with the 16S rDNA and the housekeeping gene rpoB sequencing technique. Our results showed high reliability of identification by MALDI-TOF MS at the species level covering 92.8% of cases (13 isolates) with a score greater than 2. MALDI-TOF MS and bioinformatic analysis are tools that can substitute the identification by simple sequencing using the 16S and rpoB rDNA genes.
The second part consists of evaluating the efficacy of the enzyme SsoPox-W263I, to prevent the establishment of virulence in three strains of Pseudomonas aeruginosa isolated from pneumonic calves, the model strain P. aeruginosa PAO1 and three human strains. For this, we evaluated in vitro the amount of pyocyanin, the proteolytic activity and the biofilm. In the presence of SsoPox W263I, we observed a decrease in proteolytic activity and the production of pyocyanin in 100% of the isolates. Biofilm synthesis decreased in 86% of isolates. The enzyme SsoPox W263I by
inhibition of quorum sensing attenuates the pathogenic power of P. aeruginosa. It can therefore be a promising alternative to conventional treatments.
|
| Diplôme : |
Doctorat |
| En ligne : |
../theses/biologie/GUE7255.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=10847 |
|
Apport des techniques moléculaires et bioinformatiques pour l'identification et la caractérisation fonctionnelle de germes causant les mammites subcliniques et les infections respiratoires chez les bovins à Constantine et à Batna.
