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Auteur Dalila Naimi |
Documents disponibles écrits par cet auteur (8)
Affiner la recherche Interroger des sources externesAnalyse de l’ activation de la voie PI3K/AKT dans le lymphome folliculaire / Ouardia Imene Yahiaoui-Bentounsi
Titre : Analyse de l’ activation de la voie PI3K/AKT dans le lymphome folliculaire Type de document : texte imprimé Auteurs : Ouardia Imene Yahiaoui-Bentounsi, Auteur ; Luc Xerri, Directeur de thèse ; Dalila Naimi, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 119 f. Format : 30 cm. Note générale : Doctorat 3éme cycle LMD
2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : lymphome folliculaire mutations PIK3CA phospho-AKT PTEN Follicular Lymphoma PIK3CA mutations pAKT الأورام اللمفاوية طفرات Index. décimale : 570 Sciences de la vie. Biologie Résumé : The phosphoinositide 3- kinase (PI3K) pathway is involved in the growth of various human cancers, including lymphoid malignancies. However its role in the pathogenesis of follicular lymphoma (FL) has not been yet described.
The PhD work focuses on the study of alterations in the PI3K/AKT pathway in follicular lymphoma, in order to provide a new therapeutic target.
To clarify this point, biopsy tissue samples from 38 human FL cases were investigated for PIK3CA somatic mutations in exons 9 and 20 using Sanger sequencing. The same samples were analyzed using western blotting and immunohistochemistry to detect expression of AKT, phosphorylated AKT (pAKT), and PTEN proteins. Two cases of benign lymphadenitis were used as controls.
AKT expression was present in all FL and lymphadenitis cases. 14/38 (37%) FL and 2/2 lymphadenitis cases expressed pAKT. 9/38 (24%) FL samples showed high level of pAKT, whereas 5/38 (13%) FL cases and 2/2 benign lymphadenitis samples expressed pAKT at low level. PTEN expression was observed in 30/38 (79%) FL and 2/2 benign lymphadenitis cases, whereas 8/38 (21%) of FL cases showed loss of PTEN expression. In addition, 3 cases with positive pAKT did not express PTEN. PIK3CA mutations were not detected in any sample.
These data suggest that the PI3K/AKT signaling pathway could be activated in a subset of FL cases, due to either AKT phosphorylation or PTEN downregulation, in the absence of PIK3CA mutations.Diplôme : Doctorat En ligne : ../theses/biologie/YAH6620.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9739 Analyse de l’ activation de la voie PI3K/AKT dans le lymphome folliculaire [texte imprimé] / Ouardia Imene Yahiaoui-Bentounsi, Auteur ; Luc Xerri, Directeur de thèse ; Dalila Naimi, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 119 f. ; 30 cm.
Doctorat 3éme cycle LMD
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : lymphome folliculaire mutations PIK3CA phospho-AKT PTEN Follicular Lymphoma PIK3CA mutations pAKT الأورام اللمفاوية طفرات Index. décimale : 570 Sciences de la vie. Biologie Résumé : The phosphoinositide 3- kinase (PI3K) pathway is involved in the growth of various human cancers, including lymphoid malignancies. However its role in the pathogenesis of follicular lymphoma (FL) has not been yet described.
The PhD work focuses on the study of alterations in the PI3K/AKT pathway in follicular lymphoma, in order to provide a new therapeutic target.
To clarify this point, biopsy tissue samples from 38 human FL cases were investigated for PIK3CA somatic mutations in exons 9 and 20 using Sanger sequencing. The same samples were analyzed using western blotting and immunohistochemistry to detect expression of AKT, phosphorylated AKT (pAKT), and PTEN proteins. Two cases of benign lymphadenitis were used as controls.
AKT expression was present in all FL and lymphadenitis cases. 14/38 (37%) FL and 2/2 lymphadenitis cases expressed pAKT. 9/38 (24%) FL samples showed high level of pAKT, whereas 5/38 (13%) FL cases and 2/2 benign lymphadenitis samples expressed pAKT at low level. PTEN expression was observed in 30/38 (79%) FL and 2/2 benign lymphadenitis cases, whereas 8/38 (21%) of FL cases showed loss of PTEN expression. In addition, 3 cases with positive pAKT did not express PTEN. PIK3CA mutations were not detected in any sample.
These data suggest that the PI3K/AKT signaling pathway could be activated in a subset of FL cases, due to either AKT phosphorylation or PTEN downregulation, in the absence of PIK3CA mutations.Diplôme : Doctorat En ligne : ../theses/biologie/YAH6620.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9739 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité YAH/6621 YAH/6621 Thèse Bibliothèque principale Thèses Disponible Documents numériques
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Titre : Caractérisation et étude des propriétés immunomodulatrices, anti-inflammatoires et anti-tumorales des molécules bioactives extraites d’Helix aspersa : application dans la prévention du cancer colorectal expérimental chimio induit. Type de document : texte imprimé Auteurs : Meriem Kouachi, Auteur ; Dalila Naimi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 125 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles.
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Helix aspersa bave homogénat extrait aqueux immunostimulation inflammation prolifération tumorale slime homogenate aqueous extract tumor proliferation الحلزون هيليكس اسبيرس لعاب، جناسة مستخلص المائي تحفيزمناعي التهاب ورم Index. décimale : 570 Sciences de la vie. Biologie Résumé : A competent immune system is capable of detecting and eliminating not only viruses and bacteria, but also cancer cells. However, these cells can develop an escape strategy allowing the formation of malignant tumor following immunosuppression. It is therefore necessary to look for new anti-tumor drugs with an immunostimulatory potential. Research in this field focuses on the extraction and purification of these molecules from medicinal plants. However, the aim of our work is to seek and demonstrate the immunomodulatory effect of animal extracts: the Helix aspersa snail, a species widely used and widely consumed in the Mediterranean countries. Our work begins with a characterization of the extracts (slime, homogenate and aqueous extract) and an evaluation of the toxic dose in order to choose the appropriate doses to use during our experiments. The extracts showed no toxicity to experimental animals; Therefore, the doses of 20 ml / Kg of slime, 5 mg / Kg of homogenate and 300 μl / mouse of aqueous extract were chosen. The immunomodulatory effect was shown to be immunostimulatory when performing a carbon clearance test, which led us to investigate other therapeutic effects. Our extracts also exert an anti-inflammatory effect against chemo-induced experimental colitis. Untreated chronic colitis is likely to develop as a colorectal tumor. The anti-inflammatory effect of the extracts prompted us to check the antiproliferative effect. The investigation of the antitumor effect was carried out in vitro, on a culture of MCF7 mammary tumor cells and in vivo, on an experimental chemo-induced colorectal cancer. In vitro experiments showed an inhibition of tumor cell proliferation at 56.39%. In vivo, observation of the histological sections of the colonists confirmed that the extracts repaired the architecture of the colonic mucosa after the installation of the chemoinduced tumor. The Helix aspersa snail has several therapeutic effects. It would therefore be interesting to introduce it into the daily diet as a preventive or supplementary food for a curative purpose.
Diplôme : Doctorat En ligne : ../theses/biologie/KOU7168.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10589 Caractérisation et étude des propriétés immunomodulatrices, anti-inflammatoires et anti-tumorales des molécules bioactives extraites d’Helix aspersa : application dans la prévention du cancer colorectal expérimental chimio induit. [texte imprimé] / Meriem Kouachi, Auteur ; Dalila Naimi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 125 f. ; 30 cm.
2 copies imprimées disponibles.
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Helix aspersa bave homogénat extrait aqueux immunostimulation inflammation prolifération tumorale slime homogenate aqueous extract tumor proliferation الحلزون هيليكس اسبيرس لعاب، جناسة مستخلص المائي تحفيزمناعي التهاب ورم Index. décimale : 570 Sciences de la vie. Biologie Résumé : A competent immune system is capable of detecting and eliminating not only viruses and bacteria, but also cancer cells. However, these cells can develop an escape strategy allowing the formation of malignant tumor following immunosuppression. It is therefore necessary to look for new anti-tumor drugs with an immunostimulatory potential. Research in this field focuses on the extraction and purification of these molecules from medicinal plants. However, the aim of our work is to seek and demonstrate the immunomodulatory effect of animal extracts: the Helix aspersa snail, a species widely used and widely consumed in the Mediterranean countries. Our work begins with a characterization of the extracts (slime, homogenate and aqueous extract) and an evaluation of the toxic dose in order to choose the appropriate doses to use during our experiments. The extracts showed no toxicity to experimental animals; Therefore, the doses of 20 ml / Kg of slime, 5 mg / Kg of homogenate and 300 μl / mouse of aqueous extract were chosen. The immunomodulatory effect was shown to be immunostimulatory when performing a carbon clearance test, which led us to investigate other therapeutic effects. Our extracts also exert an anti-inflammatory effect against chemo-induced experimental colitis. Untreated chronic colitis is likely to develop as a colorectal tumor. The anti-inflammatory effect of the extracts prompted us to check the antiproliferative effect. The investigation of the antitumor effect was carried out in vitro, on a culture of MCF7 mammary tumor cells and in vivo, on an experimental chemo-induced colorectal cancer. In vitro experiments showed an inhibition of tumor cell proliferation at 56.39%. In vivo, observation of the histological sections of the colonists confirmed that the extracts repaired the architecture of the colonic mucosa after the installation of the chemoinduced tumor. The Helix aspersa snail has several therapeutic effects. It would therefore be interesting to introduce it into the daily diet as a preventive or supplementary food for a curative purpose.
Diplôme : Doctorat En ligne : ../theses/biologie/KOU7168.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10589 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KOU/7168 KOU/7168 Thèse Bibliothèque principale Thèses Disponible
Titre : comparison des thérapeutiques d'extrait brut d'Helix aspersa sur le remodelage de la muqueuse intestinale de rat : soumis à une inflammation chimio-induite associée ou non à une hyperhomocysteinémie. Type de document : texte imprimé Auteurs : souad Lamda, Auteur ; Dalila Naimi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 245 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieIndex. décimale : 570 Sciences de la vie. Biologie Diplôme : Doctorat en sciences En ligne : ../theses/biologie/LAM7275.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10890 comparison des thérapeutiques d'extrait brut d'Helix aspersa sur le remodelage de la muqueuse intestinale de rat : soumis à une inflammation chimio-induite associée ou non à une hyperhomocysteinémie. [texte imprimé] / souad Lamda, Auteur ; Dalila Naimi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 245 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieIndex. décimale : 570 Sciences de la vie. Biologie Diplôme : Doctorat en sciences En ligne : ../theses/biologie/LAM7275.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10890 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAM/7275 LAM/7275 Thèse Bibliothèque principale Thèses Disponible
Titre : Effets de l’homocystéine sur les processus angiogéniques et l’invasion tumorale du tissu endothélialin vivo et in vitro Type de document : texte imprimé Auteurs : Chérifa Aggoun, Auteur ; Dalila Naimi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2015 Importance : 120 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : تولد الأوعية غزو الأورام عامل النمو الباطني الوعائية مسارات الإشارات الجرذ
Homocystéine Angiogenèse Invasion tumorale VEFG-A Voies de signalisation Rat HUVECs Homocysteine Angiogenesis Tumor invasion Signaling pathways Rat
Physiologie Cellulaire et Moléculaire Signalisation et CancerIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Angiogenesis is the formation of new blood vessels from pre-existing vessels, existing in many pathophysiological conditions, including tumorogenesis. In the activation of the endothelial cell (EC),VEGF-A (vascular endothelial growth factor) is the decisive stage of the process. Since homocysteine (Hcy) has an angiotoxic power, the aim of this work is to assess (in vivo and in vitro studies) the effect of this molecule on angiogenesis and tumor invasion involving VEGF and its two powerful signaling effectors: the HIF(hypoxia –inducible factor )
-1α and the OPN (Osteopontin).
In the in vivo study, the conception of our Hyperhomocysteinemia model (HH) is
confirmed by biochemical study (total Hcy, total protein, CRP and MDA) and histological one (aorta, liver and thymus). We showed that HH potentiated the presence of VEGF-A in thymus, suggesting the installation of a reparative angiogenesis effect, in the same time to an inhibition of VEGF-A and HIF-1α expressions in aortic tissue.
In the in vitro study, we evaluated the antiangiogenic effect of Hcy previously
revealed in vivo, by exposing HUVECs to cytotoxic and pathophysiological concentrations of Hcy. In fact, the first range revealed a strong inhibition of the proliferation, migration and capillary sprouting associated with senescence and apoptosis of EC. In pathophysiological range, inhibition is always dose -dependent and became completely significant in adenosine condition. Hcy seems to act by an hypomethylation mechanism. In addition, the results of qRT-PCR revealed that Hcy inhibited gene expression of VEGF-A and OPN in dosedependent manner.
This study shows a new aspect of antiangiogenic properties of Hcy, by impaired the
expression of genes involved in tumor angiogenesis.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AGG6808.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10061 Effets de l’homocystéine sur les processus angiogéniques et l’invasion tumorale du tissu endothélialin vivo et in vitro [texte imprimé] / Chérifa Aggoun, Auteur ; Dalila Naimi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2015 . - 120 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : تولد الأوعية غزو الأورام عامل النمو الباطني الوعائية مسارات الإشارات الجرذ
Homocystéine Angiogenèse Invasion tumorale VEFG-A Voies de signalisation Rat HUVECs Homocysteine Angiogenesis Tumor invasion Signaling pathways Rat
Physiologie Cellulaire et Moléculaire Signalisation et CancerIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Angiogenesis is the formation of new blood vessels from pre-existing vessels, existing in many pathophysiological conditions, including tumorogenesis. In the activation of the endothelial cell (EC),VEGF-A (vascular endothelial growth factor) is the decisive stage of the process. Since homocysteine (Hcy) has an angiotoxic power, the aim of this work is to assess (in vivo and in vitro studies) the effect of this molecule on angiogenesis and tumor invasion involving VEGF and its two powerful signaling effectors: the HIF(hypoxia –inducible factor )
-1α and the OPN (Osteopontin).
In the in vivo study, the conception of our Hyperhomocysteinemia model (HH) is
confirmed by biochemical study (total Hcy, total protein, CRP and MDA) and histological one (aorta, liver and thymus). We showed that HH potentiated the presence of VEGF-A in thymus, suggesting the installation of a reparative angiogenesis effect, in the same time to an inhibition of VEGF-A and HIF-1α expressions in aortic tissue.
In the in vitro study, we evaluated the antiangiogenic effect of Hcy previously
revealed in vivo, by exposing HUVECs to cytotoxic and pathophysiological concentrations of Hcy. In fact, the first range revealed a strong inhibition of the proliferation, migration and capillary sprouting associated with senescence and apoptosis of EC. In pathophysiological range, inhibition is always dose -dependent and became completely significant in adenosine condition. Hcy seems to act by an hypomethylation mechanism. In addition, the results of qRT-PCR revealed that Hcy inhibited gene expression of VEGF-A and OPN in dosedependent manner.
This study shows a new aspect of antiangiogenic properties of Hcy, by impaired the
expression of genes involved in tumor angiogenesis.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AGG6808.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10061 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AGG/6808 AGG/6808 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude du rôle de Monoxyde d’azote (NO) dans la progression du myélome Multiple (MM) et son implication dans le développement des maladies osseuses associées au MM. Type de document : texte imprimé Auteurs : Khawla Otmani, Auteur ; Dalila Naimi, Directeur de thèse ; Nadia Boudersa, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 164 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie Animale: Immuno-Oncologie inflammation monoxyde d'azote (NO) myélome multiple (MM) le microenvironnement de MM myeloma plasma cells (MPC) Les maladies osseuses associées au MM (MBD) Marqueur pronostique de MM marqueur de remodelage osseux ostéoblaste nitric oxide (NO) multiple myeloma (MM) myeloma microenvironment (MPC)
myeloma plasma cells meyeloma bone deseases MBD Myeloma prognostic marker bone remodling marker INOS inhibitor osteoblast الالتهاب اكسيد النيتريك (NO) الورم النخاعي المتعدد (MM) الخلايا البلازمية لورم النخاعي المتعدد (MPC) امراض عظام الورم النخاعي المتعدد (MBD) مؤشرات التطور مؤشرات إعادة تشكيل العظم الاستوبلاستات ( osteoblastesIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Nitric oxide (NO) is sensitive marker of inflammation and it is widely associated with cancer development. Multiple myeloma (MM) is a hematological cancer of fully differentiated plasma cells in the bone marrow which is a favorable microenvironment for the promotion of communication between malignant plasma cells MPC (Myeloma plasma cells) and other cell types using pro-inflammatory mediators. Myeloma bone diseases (MBD) and monoclonal mmunoglobulin secretion are the most visible aspects in MM.The present work aims to study the role of (NO) as an inflammatory mediator in the progression of MM and its effect on the development of MBD. We evaluated in vivo, the NO concentration in the plasma of MM patients in the Algerian East, and its possible correlation with some prognostic marker evolution, the statue of the immune
system, and some bone remodeling markers in these patients. We have also carried out a study in vitro to evaluate NO production by myeloma microenvironment using MPC (RPMI 8226 cell lines) co-culture withe osteoblast ( MG63 cell line) and to investigate anti-proliferative and cytotoxic effect of iNOS inhibitor on the two cell lines co-culture . The results showed a significant increase of NO concentration in the plasma of MM patients comparing with the plasma of positive and negative controls (p> 0.01). Hence, the profile of NO concentration allowed the revealing of a slight relation with some prognostic and bone remolding markers. Furthermore we found a significant relationship between NO concentration and monocytes (p> 0.01) as well as granulocytes rate (p> 0.01) in the MM patients. In addition, the studies realized in vitro confirmed an important production of NO by myeloma cell line (RPMI 8226), which was also noticed in the case of the co-culture of myeloma cell line (RPMI 8226 ) with the osteoblast (MG63). This work has elucidated the role of NO in MM cancer and its effect on some manifestations of the disease, including MBD.Note de contenu : Annexes. Diplôme : Doctorat En ligne : ../theses/biologie/OTM7501.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11333 Etude du rôle de Monoxyde d’azote (NO) dans la progression du myélome Multiple (MM) et son implication dans le développement des maladies osseuses associées au MM. [texte imprimé] / Khawla Otmani, Auteur ; Dalila Naimi, Directeur de thèse ; Nadia Boudersa, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2019 . - 164 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie Animale: Immuno-Oncologie inflammation monoxyde d'azote (NO) myélome multiple (MM) le microenvironnement de MM myeloma plasma cells (MPC) Les maladies osseuses associées au MM (MBD) Marqueur pronostique de MM marqueur de remodelage osseux ostéoblaste nitric oxide (NO) multiple myeloma (MM) myeloma microenvironment (MPC)
myeloma plasma cells meyeloma bone deseases MBD Myeloma prognostic marker bone remodling marker INOS inhibitor osteoblast الالتهاب اكسيد النيتريك (NO) الورم النخاعي المتعدد (MM) الخلايا البلازمية لورم النخاعي المتعدد (MPC) امراض عظام الورم النخاعي المتعدد (MBD) مؤشرات التطور مؤشرات إعادة تشكيل العظم الاستوبلاستات ( osteoblastesIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Nitric oxide (NO) is sensitive marker of inflammation and it is widely associated with cancer development. Multiple myeloma (MM) is a hematological cancer of fully differentiated plasma cells in the bone marrow which is a favorable microenvironment for the promotion of communication between malignant plasma cells MPC (Myeloma plasma cells) and other cell types using pro-inflammatory mediators. Myeloma bone diseases (MBD) and monoclonal mmunoglobulin secretion are the most visible aspects in MM.The present work aims to study the role of (NO) as an inflammatory mediator in the progression of MM and its effect on the development of MBD. We evaluated in vivo, the NO concentration in the plasma of MM patients in the Algerian East, and its possible correlation with some prognostic marker evolution, the statue of the immune
system, and some bone remodeling markers in these patients. We have also carried out a study in vitro to evaluate NO production by myeloma microenvironment using MPC (RPMI 8226 cell lines) co-culture withe osteoblast ( MG63 cell line) and to investigate anti-proliferative and cytotoxic effect of iNOS inhibitor on the two cell lines co-culture . The results showed a significant increase of NO concentration in the plasma of MM patients comparing with the plasma of positive and negative controls (p> 0.01). Hence, the profile of NO concentration allowed the revealing of a slight relation with some prognostic and bone remolding markers. Furthermore we found a significant relationship between NO concentration and monocytes (p> 0.01) as well as granulocytes rate (p> 0.01) in the MM patients. In addition, the studies realized in vitro confirmed an important production of NO by myeloma cell line (RPMI 8226), which was also noticed in the case of the co-culture of myeloma cell line (RPMI 8226 ) with the osteoblast (MG63). This work has elucidated the role of NO in MM cancer and its effect on some manifestations of the disease, including MBD.Note de contenu : Annexes. Diplôme : Doctorat En ligne : ../theses/biologie/OTM7501.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11333 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité OTM/7501 OTM/7501 Thèse Bibliothèque principale Thèses Disponible
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