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Auteur Abdelouahab Yahia |
Documents disponibles écrits par cet auteur (2)
Affiner la recherche Interroger des sources externesCaractérisation morphologique, biologique et moléculaire de quelques variétés de figuier (Ficus carica L.). / Hakima Belattar
Titre : Caractérisation morphologique, biologique et moléculaire de quelques variétés de figuier (Ficus carica L.). Type de document : texte imprimé Auteurs : Hakima Belattar, Auteur ; Abdelouahab Yahia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 160 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie et Ecologie Végétale:Sciences Biologiques Biologie et Physiologie Végétale:Biodiversité et Production Végétale Ficus carica L. polyphénoles activité antibactérienne variations génétique structure de la popuplation marqueurs iPBS -SSR descripteurs polyphenols antibacterial activity genetic variation popuplation
structure iPBS-SSR markers descriptors المركبات الفینولیة النشاط المضاد للبكتیریا التباین الوراثي بنیة المجموعة المعلمات اب ب س- س س ر واصفاتIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
Ficus carica L. is one of the first fruit species cultivated in the world, this species underwent a morphological and agronomical evolution leading to an extensive diversit and to two distinct morphotypes according to the use of figs (fresh and dry). This aims of this study were to make a morphological, biochemical and molecular characterization of cultivars tested. The results were proved an important phenotypic variability of the fig heritage according to the descriptors of IPGRI (International Plant Genetic Resources Institute) and CIHEAM (Centre International de Hautes Etudes Agronomiques Méditerranéennes) (2003). Morphological study has shown that the fruit characters appear to be more discriminating than those of the leaf. The biochemical study of cultivars tested by characterized them with phytochemical screening and evaluating their antimicrobial activities. The preliminary evaluation of the phytochemical composition of the different treated parts made it possible to highlight the presence of some chemical groups. We have shown that aqueous methanol is the best extractor of secondary metabolites. The in vitro evaluation of the antibacterial activity of the methanolic extract of F.carica leaves showed a high antibacterial activity towards Kleibseilla and E. coli and extracts obtained from fruits of F.carica were inactive towards all tested bacterial strains. Similarly, a tendency was shown indicating that the richest extracts on flavonoids exhibit the higher antibacterial activities. Molecular analysis of the genetic diversity of Algerian and Turkish fig trees based on twenty three pairs of iBPS primers and sixteen pairs of SSR primers allowed us to count a total of 63 amplified DNA fragments for iPBS markers and 25 Alleles for SSR markers were identified with an average of 2.7 and 1.6 per primer, respectively. The average value of the polymorphic information content (PIC) for the iPBS markers (0.73) was higher than that of the SSR markers (0.69). Applying the neighbor-joining method to the combined iPBSretrotransposon and SSR data, the fig genotypes were clustered into two groups. We were able to highlight the diversity within the collection as well as the rapprochement between the Algerian and Turkish pool. Therefore, this study opens great prospects for the integration of these genetic resources and their appropriate use in new breeding programs for fig trees in countries like Algeria for their optimized management. Moreover, the fig tree, with its multiple roles of valorization of the agricultural lands and fight against the erosion, of the fixation of the populations in the arid zones, constitutes the main fruit speculation which can guarantee the sustainable development of these zones and the creation of a productive and sustainable agro-system.Note de contenu : Annexe. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BEL7460.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11291 Caractérisation morphologique, biologique et moléculaire de quelques variétés de figuier (Ficus carica L.). [texte imprimé] / Hakima Belattar, Auteur ; Abdelouahab Yahia, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2019 . - 160 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie et Ecologie Végétale:Sciences Biologiques Biologie et Physiologie Végétale:Biodiversité et Production Végétale Ficus carica L. polyphénoles activité antibactérienne variations génétique structure de la popuplation marqueurs iPBS -SSR descripteurs polyphenols antibacterial activity genetic variation popuplation
structure iPBS-SSR markers descriptors المركبات الفینولیة النشاط المضاد للبكتیریا التباین الوراثي بنیة المجموعة المعلمات اب ب س- س س ر واصفاتIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
Ficus carica L. is one of the first fruit species cultivated in the world, this species underwent a morphological and agronomical evolution leading to an extensive diversit and to two distinct morphotypes according to the use of figs (fresh and dry). This aims of this study were to make a morphological, biochemical and molecular characterization of cultivars tested. The results were proved an important phenotypic variability of the fig heritage according to the descriptors of IPGRI (International Plant Genetic Resources Institute) and CIHEAM (Centre International de Hautes Etudes Agronomiques Méditerranéennes) (2003). Morphological study has shown that the fruit characters appear to be more discriminating than those of the leaf. The biochemical study of cultivars tested by characterized them with phytochemical screening and evaluating their antimicrobial activities. The preliminary evaluation of the phytochemical composition of the different treated parts made it possible to highlight the presence of some chemical groups. We have shown that aqueous methanol is the best extractor of secondary metabolites. The in vitro evaluation of the antibacterial activity of the methanolic extract of F.carica leaves showed a high antibacterial activity towards Kleibseilla and E. coli and extracts obtained from fruits of F.carica were inactive towards all tested bacterial strains. Similarly, a tendency was shown indicating that the richest extracts on flavonoids exhibit the higher antibacterial activities. Molecular analysis of the genetic diversity of Algerian and Turkish fig trees based on twenty three pairs of iBPS primers and sixteen pairs of SSR primers allowed us to count a total of 63 amplified DNA fragments for iPBS markers and 25 Alleles for SSR markers were identified with an average of 2.7 and 1.6 per primer, respectively. The average value of the polymorphic information content (PIC) for the iPBS markers (0.73) was higher than that of the SSR markers (0.69). Applying the neighbor-joining method to the combined iPBSretrotransposon and SSR data, the fig genotypes were clustered into two groups. We were able to highlight the diversity within the collection as well as the rapprochement between the Algerian and Turkish pool. Therefore, this study opens great prospects for the integration of these genetic resources and their appropriate use in new breeding programs for fig trees in countries like Algeria for their optimized management. Moreover, the fig tree, with its multiple roles of valorization of the agricultural lands and fight against the erosion, of the fixation of the populations in the arid zones, constitutes the main fruit speculation which can guarantee the sustainable development of these zones and the creation of a productive and sustainable agro-system.Note de contenu : Annexe. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BEL7460.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11291 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEL/7460 BEL/7460 Thèse Bibliothèque principale Thèses Disponible
Titre : Comportements biologique, physiologique, biochimique et l’activité biologique de quatre variétés d’olivier (Olea europeae L.) dans l’Est Algérien. Type de document : texte imprimé Auteurs : Sara Himour ép Cherrier, Auteur ; Abdelouahab Yahia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 209 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Olea europaea L. morphologie physiologie HPLC Oleuropéine activité biologique antioxydante anticoagulante antibactérienne morphology physiology Oleuropein biological activity antioxidant anticoagulant antibacterial اولیااوروبیا Lمورفولوجیا، فیزیولوجیة HPLCأولیوروبین النشاط البیولوجي مضادات الأكسدة مضاد للتخثر مضاد
للبكتیریاIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The present study focuses on the biological, physiological and biochemical behavior of the cultivated olive tree, Olea europaea L., represented by four varieties sampled at the Maazouzi Lakhdar station, Mila: Sigoise, Rougette, Chemlel and Dathier. The morphological characterizations (quantitative and qualitative), relating to the leaves, fruits and cores, described in the descriptors of the International Olive Council (IOC, 2003), revealed differences between the varieties. The set of morphological traits assessed during the three years of study (2013-2014- 2015) revealed a significant difference between the varieties studied confirmed by coefficients of
variation ranging from 2.81% to 39.14%. Positive correlations between mean precipitation, duration of insolations and morphological characters ranged from r = 0.839 to 0.946, and negative for fruit width and nuclei with temperature.
The results of the analysis of the biochemical characterization data revealed a great difference between the varieties. The most important lipid content was recorded in Sigoise leaf and fruit, Rougette gave the best sugar content in the leaves, as well as in Chemlel fruits. The qualitative analysis performed by phytochemical screening revealed the presence of several families of natural compounds in both organs. Rougette has been the dominant variety for total leaf and fruit phenol and flavonoid content, and Dathier for tannins. Quantitative analyzes of the phenolic compounds identified in the hydromethanol extracts by HPLC identified four
compounds: fumaric acid, gallic acid, oleuropein and caffeic acid, seven compounds in the leaves: fumaric acid, gallic acid, oleuropein, 4-hydroxybenzoic acid acid, caffeic acid, ellagic acid and t-3 hydroxyroamic acid, with a significant dominance of Oleuropein.
The evaluation of the biological potential of leaf and fruit extracts, revealed a significant anticoagulant activity in Chemlel for both organs and an antioxidant power of interest in Rougette leaves and Sigois fruits. All extracts have an important antibacterial power, extracts of leaf at Rougette gave a better zone of inhibition against S. aureus.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/HIM7315.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10931 Comportements biologique, physiologique, biochimique et l’activité biologique de quatre variétés d’olivier (Olea europeae L.) dans l’Est Algérien. [texte imprimé] / Sara Himour ép Cherrier, Auteur ; Abdelouahab Yahia, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 209 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Olea europaea L. morphologie physiologie HPLC Oleuropéine activité biologique antioxydante anticoagulante antibactérienne morphology physiology Oleuropein biological activity antioxidant anticoagulant antibacterial اولیااوروبیا Lمورفولوجیا، فیزیولوجیة HPLCأولیوروبین النشاط البیولوجي مضادات الأكسدة مضاد للتخثر مضاد
للبكتیریاIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The present study focuses on the biological, physiological and biochemical behavior of the cultivated olive tree, Olea europaea L., represented by four varieties sampled at the Maazouzi Lakhdar station, Mila: Sigoise, Rougette, Chemlel and Dathier. The morphological characterizations (quantitative and qualitative), relating to the leaves, fruits and cores, described in the descriptors of the International Olive Council (IOC, 2003), revealed differences between the varieties. The set of morphological traits assessed during the three years of study (2013-2014- 2015) revealed a significant difference between the varieties studied confirmed by coefficients of
variation ranging from 2.81% to 39.14%. Positive correlations between mean precipitation, duration of insolations and morphological characters ranged from r = 0.839 to 0.946, and negative for fruit width and nuclei with temperature.
The results of the analysis of the biochemical characterization data revealed a great difference between the varieties. The most important lipid content was recorded in Sigoise leaf and fruit, Rougette gave the best sugar content in the leaves, as well as in Chemlel fruits. The qualitative analysis performed by phytochemical screening revealed the presence of several families of natural compounds in both organs. Rougette has been the dominant variety for total leaf and fruit phenol and flavonoid content, and Dathier for tannins. Quantitative analyzes of the phenolic compounds identified in the hydromethanol extracts by HPLC identified four
compounds: fumaric acid, gallic acid, oleuropein and caffeic acid, seven compounds in the leaves: fumaric acid, gallic acid, oleuropein, 4-hydroxybenzoic acid acid, caffeic acid, ellagic acid and t-3 hydroxyroamic acid, with a significant dominance of Oleuropein.
The evaluation of the biological potential of leaf and fruit extracts, revealed a significant anticoagulant activity in Chemlel for both organs and an antioxidant power of interest in Rougette leaves and Sigois fruits. All extracts have an important antibacterial power, extracts of leaf at Rougette gave a better zone of inhibition against S. aureus.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/HIM7315.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10931 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité HIM/7315 HIM/7315 Thèse Bibliothèque principale Thèses Disponible
