| Titre : |
Etude de la biodiversité des actinobactéries dans quelques eaux thermales de la région Est de l’Algérie. |
| Type de document : |
texte imprimé |
| Auteurs : |
Maissa Medjemadj, Auteur ; Allaoueddine Boudemagh, Directeur de thèse |
| Mention d'édition : |
16/12/21 |
| Editeur : |
جامعة الإخوة منتوري قسنطينة |
| Année de publication : |
2021 |
| Importance : |
153 f. |
| Format : |
30 cm. |
| Note générale : |
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
|
| Langues : |
Français (fre) |
| Catégories : |
Français - Anglais
Biologie
|
| Tags : |
Microbiologie : Écologie Microbienne Eaux thermales Actinobacteria Streptomyces Activités enzymatiques Activités antimicrobiennes Thermal waters Enzymatic activities Antimicrobial activities الينابيع الساخنة اكتينوبكتيريا ستربتوميساس الانشطة الانزيمية انشطة المضادات الحيوية |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
The presence and biodiversity of Actinobacteria in four Algerian hot springs was explored using water samples from four thermal springs located in northeastern Algeria. Twenty-three morphologically distinct isolates were isolated and identified. Analysis of the 16S rRNA sequences showed that the isolates were assigned to the Streptomycetaceae, Nocardiaceae and Microbacteriaceae families, the majority being affiliated to the genus Streptomyces. The metabolic diversity of the strains was also explored using the api® galleries (BioMérieux). From the batch of isolated actinobacteria, 4 actinobacteria were selected and studied further. This characterization concerns spore morphology by electron microscopic examination, tolerance to different pH and sodium chloride concentrations, growth at different temperatures and G+C content and chemotaxonomy. The in-depth study revealed that these strains are thermotolerant, halotolerant bacteria and prefer an alkaline pH. The G+C content of these strains is quite low and ranges from 41% to 66%. Their cell walls are type I and their phospholipid profiles are type PII. The characteristic cellular fatty acids detected are AnteisoC15: 0, Iso-C15: 0, Iso-C16: 0, Anteiso-C17: 0 and Iso-C17: 0.The menaquinones present mainly are MK-9 (H6) and MK-9 (H8). These strains have several enzymes which are biotechnologicaly very interesting, such as cellulases, xylanases, lipases and proteases. The antimicrobial activity of these 4 strains was carried out against 6 bacteria and 2 filamentous fungi. These facultative thermophiles actinobacteria are capable of producing antagonist antibiotic compounds against at least one test bacteria studied.
|
| Note de contenu : |
Annexes.
|
| Diplôme : |
Doctorat |
| En ligne : |
../theses/biologie/MED7845.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=11748 |
Etude de la biodiversité des actinobactéries dans quelques eaux thermales de la région Est de l’Algérie. [texte imprimé] / Maissa Medjemadj, Auteur ; Allaoueddine Boudemagh, Directeur de thèse . - 16/12/21 . - جامعة الإخوة منتوري قسنطينة, 2021 . - 153 f. ; 30 cm. Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français ( fre)
| Catégories : |
Français - Anglais
Biologie
|
| Tags : |
Microbiologie : Écologie Microbienne Eaux thermales Actinobacteria Streptomyces Activités enzymatiques Activités antimicrobiennes Thermal waters Enzymatic activities Antimicrobial activities الينابيع الساخنة اكتينوبكتيريا ستربتوميساس الانشطة الانزيمية انشطة المضادات الحيوية |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
The presence and biodiversity of Actinobacteria in four Algerian hot springs was explored using water samples from four thermal springs located in northeastern Algeria. Twenty-three morphologically distinct isolates were isolated and identified. Analysis of the 16S rRNA sequences showed that the isolates were assigned to the Streptomycetaceae, Nocardiaceae and Microbacteriaceae families, the majority being affiliated to the genus Streptomyces. The metabolic diversity of the strains was also explored using the api® galleries (BioMérieux). From the batch of isolated actinobacteria, 4 actinobacteria were selected and studied further. This characterization concerns spore morphology by electron microscopic examination, tolerance to different pH and sodium chloride concentrations, growth at different temperatures and G+C content and chemotaxonomy. The in-depth study revealed that these strains are thermotolerant, halotolerant bacteria and prefer an alkaline pH. The G+C content of these strains is quite low and ranges from 41% to 66%. Their cell walls are type I and their phospholipid profiles are type PII. The characteristic cellular fatty acids detected are AnteisoC15: 0, Iso-C15: 0, Iso-C16: 0, Anteiso-C17: 0 and Iso-C17: 0.The menaquinones present mainly are MK-9 (H6) and MK-9 (H8). These strains have several enzymes which are biotechnologicaly very interesting, such as cellulases, xylanases, lipases and proteases. The antimicrobial activity of these 4 strains was carried out against 6 bacteria and 2 filamentous fungi. These facultative thermophiles actinobacteria are capable of producing antagonist antibiotic compounds against at least one test bacteria studied.
|
| Note de contenu : |
Annexes.
|
| Diplôme : |
Doctorat |
| En ligne : |
../theses/biologie/MED7845.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=11748 |
|
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