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Auteur Hanane Aissaoui |
Documents disponibles écrits par cet auteur (2)
Affiner la recherche Interroger des sources externesInvestigation phytochimique de plantes médicinales sahariennes – Activité biologique. / Hanane Aissaoui
Titre : Investigation phytochimique de plantes médicinales sahariennes – Activité biologique. Type de document : texte imprimé Auteurs : Hanane Aissaoui, Auteur ; Ratiba Mekkiou, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 258 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Heliotropium bacciferum Lifago dielsii terpènes nor-isoprenoïdes flavonoïdes polyphénols activité antioxydante effet cytotoxique terpenes nor-isoprenoïds flavonoïds polyphenols antioxidant activity cytotoxic effect تربينات أحاديةافلافونويد بوليفينول نشاط مضاد للكسدة التأثير السمي للخلايا Index. décimale : 540 Chimie et sciences connexes Résumé : This work is devoted to the phytochemical and biological study of two endemic Saharan medicinal plants, Heliotropium bacciferum Forssk. (Boraginaceae) and Lifago dielsii Schwein. & Musch. (Asteraceae).
This phytochemical study led the isolation and identification of twenty products, seventeen of which were monoterpenes megastigans and phenolic type, were obtained from the chloroform and methanol extracts of the aerial parts of Heliotropium bacciferum Forssk.
While three glycosylated flavonoids were obtained from the ethyl acetate extract of the aerial parts of Lifago dielsii Schwein & Musch. The structures of the isolated products were elucidated mainly by the use of NMR techniques (1H, 13C, COSY, HSQC and HMBC), UV spectrophotometry and by comparison with those reported in the literature.
The quantification of the total phenolic content of the MeOH extract of H. bacciferum was carried out using the Folin-Ciocalteu method. This step showed a polyphenolic content of 68.0 mgGAE/g extract. This extract was evaluated for its antioxidant activity towards the free radical DPPH and the radical ABTS. The results showed that this extract exhibited a significant activity and is dependent on the concentration in vitro with respect to the DPPH
radicals (IC50 70.912 μg/ml), and ABTS (IC50 1.466 μg/ml), related to the presence of the phenolic derivatives.
Both extracts and some isolated products of H. bacciferum were tested for their cytotoxic effect in human cancer cell lines (HCT-116, DLD1 and HDFa). Only the chloroform extract showed a significant and concentration-dependent inhibitory effect on the growth of IC50 treated cancer cell lines of 0.095 mg/ml on HCT116 and 0.062 mg/ml on DLD1.
The quantification of total phenols and total flavonoids contents of the CHCl3, EtOAc, nBuOH extracts and of the methanol insoluble part (MeOH) of the aerial parts of L. dielsii was carried out using colometric methods. This study showed a phenolic content more important in the EtOAc extract. However, total flavonoids content was higher in n-BuOH extract (88.81%). The antioxidant activity of L. dielsii extracts was evaluated by the DPPH free radical scavenging and the lipid peroxidation inhibition (LPO) assays. The EtOAc extract has the strongest effect on DPPH with (IC50 = 47.80 ± 2.20 μg/ml) and the inhibition of lipid peroxidation (IC50 = 113.24 ± 0.65 μg/ml).
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/AIS7285.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10900 Investigation phytochimique de plantes médicinales sahariennes – Activité biologique. [texte imprimé] / Hanane Aissaoui, Auteur ; Ratiba Mekkiou, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 258 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Heliotropium bacciferum Lifago dielsii terpènes nor-isoprenoïdes flavonoïdes polyphénols activité antioxydante effet cytotoxique terpenes nor-isoprenoïds flavonoïds polyphenols antioxidant activity cytotoxic effect تربينات أحاديةافلافونويد بوليفينول نشاط مضاد للكسدة التأثير السمي للخلايا Index. décimale : 540 Chimie et sciences connexes Résumé : This work is devoted to the phytochemical and biological study of two endemic Saharan medicinal plants, Heliotropium bacciferum Forssk. (Boraginaceae) and Lifago dielsii Schwein. & Musch. (Asteraceae).
This phytochemical study led the isolation and identification of twenty products, seventeen of which were monoterpenes megastigans and phenolic type, were obtained from the chloroform and methanol extracts of the aerial parts of Heliotropium bacciferum Forssk.
While three glycosylated flavonoids were obtained from the ethyl acetate extract of the aerial parts of Lifago dielsii Schwein & Musch. The structures of the isolated products were elucidated mainly by the use of NMR techniques (1H, 13C, COSY, HSQC and HMBC), UV spectrophotometry and by comparison with those reported in the literature.
The quantification of the total phenolic content of the MeOH extract of H. bacciferum was carried out using the Folin-Ciocalteu method. This step showed a polyphenolic content of 68.0 mgGAE/g extract. This extract was evaluated for its antioxidant activity towards the free radical DPPH and the radical ABTS. The results showed that this extract exhibited a significant activity and is dependent on the concentration in vitro with respect to the DPPH
radicals (IC50 70.912 μg/ml), and ABTS (IC50 1.466 μg/ml), related to the presence of the phenolic derivatives.
Both extracts and some isolated products of H. bacciferum were tested for their cytotoxic effect in human cancer cell lines (HCT-116, DLD1 and HDFa). Only the chloroform extract showed a significant and concentration-dependent inhibitory effect on the growth of IC50 treated cancer cell lines of 0.095 mg/ml on HCT116 and 0.062 mg/ml on DLD1.
The quantification of total phenols and total flavonoids contents of the CHCl3, EtOAc, nBuOH extracts and of the methanol insoluble part (MeOH) of the aerial parts of L. dielsii was carried out using colometric methods. This study showed a phenolic content more important in the EtOAc extract. However, total flavonoids content was higher in n-BuOH extract (88.81%). The antioxidant activity of L. dielsii extracts was evaluated by the DPPH free radical scavenging and the lipid peroxidation inhibition (LPO) assays. The EtOAc extract has the strongest effect on DPPH with (IC50 = 47.80 ± 2.20 μg/ml) and the inhibition of lipid peroxidation (IC50 = 113.24 ± 0.65 μg/ml).
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/AIS7285.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10900 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AIS/7285 AIS/7285 Thèse Bibliothèque principale Thèses Disponible
Titre : Recherche et détermination structurale des métabolites secondaires de type flavonique d'une espèce de la famille des verbenacées Type de document : texte imprimé Auteurs : Hanane Aissaoui ; Univ. de Constantine, Éditeur scientifique ; R. Mekkiou, Directeur de thèse Année de publication : 2010 Importance : 96 f. Note générale : 01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Flavonoides Vitex agnus-castus Verbenacée Index. décimale : 540 Chimie et sciences connexes Résumé : This work is a modest contribution to knowledge the verbenaceae family through the Vitex genus in particularly Vitex agnus-castus species which is recognized by its pharmalogical properties, it is also used in traditional medicine as to calm the rheumatism pains in south and west south region of the country.
This study relates to the phytochemical investigation of the semi p olar phase of the aqueous-alcoholic extracts of the leaves of Vitex agnus- castus species. This contribution led to the isolation of seven products in the pure state, from which, we have established the structure of
three in still now: two flavones and one flavonol.
9 5,3'-dihydroxy 3, 6, 7,4'-tetramethoxyflavone (Casticin)
9 5,3’,4’ -trihydroxy 7-methoxyflavone (luteolin7-methyl)
9 5,7,3',4’-tetrahydroxyflavone-7-O- β-D-glucopyranoside (luteolin 7-Oglucoside )
The elucidation of different structures was carried out by using spectroscopic methods,
especially UV spectrophotometry, MS spectrometry as well as 1H NMR, 13C NMR experiments.Diplôme : Magistère En ligne : ../theses/chimie/AIS5762.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=3712 Recherche et détermination structurale des métabolites secondaires de type flavonique d'une espèce de la famille des verbenacées [texte imprimé] / Hanane Aissaoui ; Univ. de Constantine, Éditeur scientifique ; R. Mekkiou, Directeur de thèse . - 2010 . - 96 f.
01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Flavonoides Vitex agnus-castus Verbenacée Index. décimale : 540 Chimie et sciences connexes Résumé : This work is a modest contribution to knowledge the verbenaceae family through the Vitex genus in particularly Vitex agnus-castus species which is recognized by its pharmalogical properties, it is also used in traditional medicine as to calm the rheumatism pains in south and west south region of the country.
This study relates to the phytochemical investigation of the semi p olar phase of the aqueous-alcoholic extracts of the leaves of Vitex agnus- castus species. This contribution led to the isolation of seven products in the pure state, from which, we have established the structure of
three in still now: two flavones and one flavonol.
9 5,3'-dihydroxy 3, 6, 7,4'-tetramethoxyflavone (Casticin)
9 5,3’,4’ -trihydroxy 7-methoxyflavone (luteolin7-methyl)
9 5,7,3',4’-tetrahydroxyflavone-7-O- β-D-glucopyranoside (luteolin 7-Oglucoside )
The elucidation of different structures was carried out by using spectroscopic methods,
especially UV spectrophotometry, MS spectrometry as well as 1H NMR, 13C NMR experiments.Diplôme : Magistère En ligne : ../theses/chimie/AIS5762.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=3712 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AIS/5762 AIS/5762 Thèse Bibliothèque principale Thèses Disponible
