| Titre : |
Caractérisation cytogénétique classique et moléculaire de trois espèces endémiques du genre Hedysarum L. |
| Type de document : |
texte imprimé |
| Auteurs : |
Hayet Goudjil-Benhizia, Auteur ; Nadra Khalfallah, Directeur de thèse |
| Editeur : |
constantine [Algérie] : Université Constantine 1 |
| Année de publication : |
2014 |
| Importance : |
82 f. |
| Format : |
30 cm. |
| Note générale : |
2 copies imprimées disponibles |
| Langues : |
Français (fre) |
| Catégories : |
Français - Anglais
Biologie
|
| Tags : |
caryotype chromosomes B organisation de l'hétérochromatine gènes
ribosomiques 5S et 35S méiose taille du génome karyotype B chromosomes heterochromatin organization ribosomal genes 5S
and 35S meiosis genome size النمط النووي كروموسات "B" التنظيم الهيتيروكروماتين الجينات الريبوزومية 35Sو5S المنصف الأنقسام كمية المجموع المورثي |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
The first cytogenetic data are presented for two diploid species (2n = 2x = 16): H. pallidum Desf.
and H. naudinianum Coss. and a tetraploid species (2n = 4x = 32): H. perrauderianum Coss. and
Durieu. These endemic species of Algeria and North Africa are fodder. The karyogram consists of 8 chromosome pairs; one pair is satellite in the diploid and 16 pairs among which two are satellites in the tetraploid. The constitutive heterochromatin, as thin and wide, intercalary and telomeric bands is revealed by C-banding. One to four heterochromatic B chromosomes were observed. Staining of metaphase chromosomes by chromomycin A3 shows bands of DNA rich in GC base, located in the short arm telomere pairs with satellites, which correspond to the nucleolar organizer regions. FISH
revealed the existence of a locus in diploid and two loci in the tetraploid of each family of ribosomal genes (5S and 35S). 5S signals are observed in H. pallidum (2x) in centromeric region, and in H.
naudinianum (2x) and H. perrauderianum (4x) in telomeric region. In all three species studied, the loci 35S are located on satellifère pairs in secondary constrictions and NORs. The majority of bands CMA+ are also DAPI+.The similarity of certain chromosome pairs and presence of univalents, trivalents and tetravalents suggest an autotetraploid origin of H. perrauderianum species.The genome size of 2C = 1.5 pg in H. pallidum, 3.36 pg in H. naudinianum and 6.64 pg in H.
perrauderianum. The karyotype analysis, organization of heterochromatin, ribosomal genes and
genome size, it can be concluded that H. perrauderianum is nearest of H. naudinianum than H.
pallidum.
|
| Diplôme : |
Doctorat en sciences |
| En ligne : |
../theses/biologie/GOU6471.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=9514 |
Caractérisation cytogénétique classique et moléculaire de trois espèces endémiques du genre Hedysarum L. [texte imprimé] / Hayet Goudjil-Benhizia, Auteur ; Nadra Khalfallah, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 82 f. ; 30 cm. 2 copies imprimées disponibles Langues : Français ( fre)
| Catégories : |
Français - Anglais
Biologie
|
| Tags : |
caryotype chromosomes B organisation de l'hétérochromatine gènes
ribosomiques 5S et 35S méiose taille du génome karyotype B chromosomes heterochromatin organization ribosomal genes 5S
and 35S meiosis genome size النمط النووي كروموسات "B" التنظيم الهيتيروكروماتين الجينات الريبوزومية 35Sو5S المنصف الأنقسام كمية المجموع المورثي |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
The first cytogenetic data are presented for two diploid species (2n = 2x = 16): H. pallidum Desf.
and H. naudinianum Coss. and a tetraploid species (2n = 4x = 32): H. perrauderianum Coss. and
Durieu. These endemic species of Algeria and North Africa are fodder. The karyogram consists of 8 chromosome pairs; one pair is satellite in the diploid and 16 pairs among which two are satellites in the tetraploid. The constitutive heterochromatin, as thin and wide, intercalary and telomeric bands is revealed by C-banding. One to four heterochromatic B chromosomes were observed. Staining of metaphase chromosomes by chromomycin A3 shows bands of DNA rich in GC base, located in the short arm telomere pairs with satellites, which correspond to the nucleolar organizer regions. FISH
revealed the existence of a locus in diploid and two loci in the tetraploid of each family of ribosomal genes (5S and 35S). 5S signals are observed in H. pallidum (2x) in centromeric region, and in H.
naudinianum (2x) and H. perrauderianum (4x) in telomeric region. In all three species studied, the loci 35S are located on satellifère pairs in secondary constrictions and NORs. The majority of bands CMA+ are also DAPI+.The similarity of certain chromosome pairs and presence of univalents, trivalents and tetravalents suggest an autotetraploid origin of H. perrauderianum species.The genome size of 2C = 1.5 pg in H. pallidum, 3.36 pg in H. naudinianum and 6.64 pg in H.
perrauderianum. The karyotype analysis, organization of heterochromatin, ribosomal genes and
genome size, it can be concluded that H. perrauderianum is nearest of H. naudinianum than H.
pallidum.
|
| Diplôme : |
Doctorat en sciences |
| En ligne : |
../theses/biologie/GOU6471.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=9514 |
|
Caractérisation cytogénétique classique et moléculaire de trois espèces endémiques du genre Hedysarum L.
