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L’accumulation de la proline sous déficit hydrique, synthèse et devenir après retour de l’arrosage chez les végétaux / Sarra Merabta
Titre : L’accumulation de la proline sous déficit hydrique, synthèse et devenir après retour de l’arrosage chez les végétaux : Exemple des Triticum et Hordeum. Type de document : texte imprimé Auteurs : Sarra Merabta, Auteur ; Mostefa Benlaribi, Directeur de thèse Mention d'édition : 18/12/2019 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 164 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie et Ecologie Végétale:Biotechnologies, Biologie et Environnement Biotechnologies, Biologie et Environnement:Les bases Biologiques de la Production et Biodiversité Végétale Blé (Triticum) orge(Hordeum) stress hydrique Proline chlorophylle Stress oxydatif enzymes Wheat (Triticum) Barley (Hordeum) hydrous stress Chlorophyll Oxidative stress القمح ( )Triticumالشعير ( )Hordeumالاجهاد المائي البرولين الكلوروفيل الاجهاد
التأكسد الانزيماتIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
The objective of this work consists in evaluating the effect of a hydrous deficit created using a restriction of irrigation for a period of twenty (20) days on two durum wheat genotypes (Triticum durum Desf.): Gamgoum Rekham ( GGR) and Haurani; two genotypes of common wheat (Triticum aestivum L.): Florence Aurore 8193 (FA) and Mexipak and two genotypes of barley (Hordeum vulgare L.): Manel and Saida 183 and to follow the impact of this controlled stress after 24 hours (APR1) and one week (APR2), following the return of watering. A first experiment is carried out in order to compare the relation between Proline and chlorophyll, the application of prolonged hydric stress causes an accumulation of the content of proline in the leaves of the two kinds tested compared to that of their witnesses. However, this accumulation is in favor of the Triticum kind beside the Hordeum kind with a remarkable
diversity within the different varieties. As for the total chlorophyll composition of the organs studied, it evolves in a sawtooth pattern that does not lead to any indicative trend during the various treatments. After rehydration, there is a progressive decrease in proline content (APR1 and APR2), particularly at the two barley genotypes, and any change concerning the chlorophyll content. One second experimentation is carried out on the same genotypes with the same hydric treatments but this time to determine the influence of the oxydative stress produced by the dryness on the antioxydant metabolism. We were particularly interested in the activity of the superoxyde dismutase (SOD), malonedialdéhydes (MDA) content, catalase (CAT), peroxidases content (POD), as well as the ascorbate peroxidases (APX), and the content of proteins, aiming to characterize varieties according to their defense mechanisms against the effects of free radicals induced by water stress more precisely during the most critical stage and more affected by this Lack (heading stage) and to evaluate the impact of the oxydative
defensive system (Indicators of oxidation and antioxidant enzymes activities) on the synthesis and the degradation of the proline in order to identify the elements of the signaling pathways involved, and to evaluate the effect of this stress on the synthesis of this compound since the precursor molecules and its fate after the return of watering.Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/biologie/MER7611.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11447 L’accumulation de la proline sous déficit hydrique, synthèse et devenir après retour de l’arrosage chez les végétaux : Exemple des Triticum et Hordeum. [texte imprimé] / Sarra Merabta, Auteur ; Mostefa Benlaribi, Directeur de thèse . - 18/12/2019 . - جامعة الإخوة منتوري قسنطينة, 2019 . - 164 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie et Ecologie Végétale:Biotechnologies, Biologie et Environnement Biotechnologies, Biologie et Environnement:Les bases Biologiques de la Production et Biodiversité Végétale Blé (Triticum) orge(Hordeum) stress hydrique Proline chlorophylle Stress oxydatif enzymes Wheat (Triticum) Barley (Hordeum) hydrous stress Chlorophyll Oxidative stress القمح ( )Triticumالشعير ( )Hordeumالاجهاد المائي البرولين الكلوروفيل الاجهاد
التأكسد الانزيماتIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
The objective of this work consists in evaluating the effect of a hydrous deficit created using a restriction of irrigation for a period of twenty (20) days on two durum wheat genotypes (Triticum durum Desf.): Gamgoum Rekham ( GGR) and Haurani; two genotypes of common wheat (Triticum aestivum L.): Florence Aurore 8193 (FA) and Mexipak and two genotypes of barley (Hordeum vulgare L.): Manel and Saida 183 and to follow the impact of this controlled stress after 24 hours (APR1) and one week (APR2), following the return of watering. A first experiment is carried out in order to compare the relation between Proline and chlorophyll, the application of prolonged hydric stress causes an accumulation of the content of proline in the leaves of the two kinds tested compared to that of their witnesses. However, this accumulation is in favor of the Triticum kind beside the Hordeum kind with a remarkable
diversity within the different varieties. As for the total chlorophyll composition of the organs studied, it evolves in a sawtooth pattern that does not lead to any indicative trend during the various treatments. After rehydration, there is a progressive decrease in proline content (APR1 and APR2), particularly at the two barley genotypes, and any change concerning the chlorophyll content. One second experimentation is carried out on the same genotypes with the same hydric treatments but this time to determine the influence of the oxydative stress produced by the dryness on the antioxydant metabolism. We were particularly interested in the activity of the superoxyde dismutase (SOD), malonedialdéhydes (MDA) content, catalase (CAT), peroxidases content (POD), as well as the ascorbate peroxidases (APX), and the content of proteins, aiming to characterize varieties according to their defense mechanisms against the effects of free radicals induced by water stress more precisely during the most critical stage and more affected by this Lack (heading stage) and to evaluate the impact of the oxydative
defensive system (Indicators of oxidation and antioxidant enzymes activities) on the synthesis and the degradation of the proline in order to identify the elements of the signaling pathways involved, and to evaluate the effect of this stress on the synthesis of this compound since the precursor molecules and its fate after the return of watering.Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/biologie/MER7611.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11447 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MER/7611 MER/7611 Thèse Bibliothèque principale Thèses Disponible
Titre : L'Accumulation de la proline en tant que test précoce d'adaptation au déficit hydrique et indicateur moléculaire de diversité chez Triticum aestivum L. (Blé tendre) Type de document : texte imprimé Auteurs : Chafia Zerafa, Auteur Editeur : Constantine : Université Mentouri Constantine Année de publication : 2006 Importance : 93 f. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Déficit hydrique Accumulation Proline Diversité Triticum aestivum L. Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Magistère Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1528 L'Accumulation de la proline en tant que test précoce d'adaptation au déficit hydrique et indicateur moléculaire de diversité chez Triticum aestivum L. (Blé tendre) [texte imprimé] / Chafia Zerafa, Auteur . - Constantine : Université Mentouri Constantine, 2006 . - 93 f.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Déficit hydrique Accumulation Proline Diversité Triticum aestivum L. Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Magistère Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1528 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ZER/4404 ZER/4404 Thèse Bibliothèque principale Thèses Disponible
Titre : Activité biochimique des extraits flavonoiques de la plante Ranunculus repens L. : effet sur le diabLte expérimental et l'hépatotoxicité induite par l'Epirubicine. Type de document : texte imprimé Auteurs : Mohamed Kebieche, Auteur ; Zahia Meraihi, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2009 Importance : 129 f. Format : 30 cm. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biochimie - Microbiologie: Biochimie diabète flavonoïdes Stress oxydant Alloxane Ranunculus repens L. Effet antidiabétique Epirubicine Hépatotoxicité Quercétine Chémoprotecteur stress oxydant flavonoids extract Ranunculus repens L alloxane antidiabetic effect épirubicin Hepatotoxicity Oxidative stress Quercetin Hepatoprotective effect. Index. décimale : 570 Sciences de la vie. Biologie Résumé : In the present study, the antihyperglycemic, anti-diabetic and antioxidant effects of the n-Butanol extract (BE) obtained from air part of Ranunculus repens L plant and quercetin were investigated in normal glucose-fed hyperglycemia, in alloxan induced diabetes and in epirubicin (EPI) toxicity of the rats. The results of this study show antihyperglycemic and antidiabetic activity of the phenolic compounds. And to elucidate a possible mechanism of action of this plant extract, the glycogen liver rate, insulin secretion and the in vitro chelating effect of glucose by plant compounds were also investigated. The flavonoids reduced significantly blood glucose in a dose-dependant manner (200,400,600 mg/kg) in both hyperglycemic and diabetic rats after oral administration as oral hypoglycemic drug, glibenclamide (2.5 mg/kg). We show that flavonoids extracts was able significantly to complex the glucose in vitro, induce insulin secretion of beta cells and to active glycogenesis in liver as shown by the abundance of glycogen granulation in liver histochemical preparation after administration of flavonoids extract. In addition these flavonoids extract and quercetin possess a chemoprotector power against malondialdehyde formation (MDA, lipid peroxidation product) and reduction of antioxidant systems such SOD, CAT and GSH in alloxan-induced diabetic rats. The equilibrium disorder of redox status after alloxan administration is reestablished by flavonoids pretreatment of alloxanised rats. The flavonoids markedly inhibited lipid peroxidation process in pancreatic cells and the proportioning of cytosolic SOD, CAT and GSH showed a significant elevation to establish equilibrium of redox status and consequently protect beta cells and prevent diabetes. The anticancer therapy with EPI produces acute hepatotoxicityby generation of free radicals. However, the oxidative status of the liver cells and mitochondria has been investigated in EPI toxicity of the rats. In this present study, we investigated in first the prooxidant effect of EPI and its implication on both hepatic cells and mitochondria function. EPI injection in rats at dose of 9 mg/kg induced hepatic dysfunction revealed by a significant increase in serum of glutamate oxaloacetate transaminases and glutamate pyruvate transaminases. Oxidative stress in liver cells and mitochondria was provoked by EPI since a statistically significant reduction of CAT, SOD and GSH levels, and significant increase of MDA level, indicator of lipid peroxidation which could perforate the biologic
membranes, were observed. In second, the protective effect of quercetin (33 mg/kg) and flavonoids extract of RRL (100 mg/kg) against oxidative stress induced by EPI was also investigated. Indeed, the pretreatment of rats with flavonoids protected liver cells and mitochondria from oxidative stress permitting, on one hand, the prevention of hepatic dysfunction on maintaining the normal level of serum transaminases following inhibition of their hepatic leakage by preventing lipid peroxidation Thus, the prevention of the cellular membranes perforation, and in the other hand maintaining also antioxidant defense system in mitochondria and cytosol of hepatocytes. Taken together, this data reveal that flavonoids extract of Ranunculus repens L extract and quercetin possess an interesting virtue to be antihyperglycemic and antidiabetic with positive action on liver glycogenesis and insulin secretion. These phenolic compounds have also antiradical and antioxidant power that confers them chemoprotection role against oxidative stress generated in pancreas cells and liver respectively by alloxan and epirubicin.
Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/biologie/KEB5525.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1794 Activité biochimique des extraits flavonoiques de la plante Ranunculus repens L. : effet sur le diabLte expérimental et l'hépatotoxicité induite par l'Epirubicine. [texte imprimé] / Mohamed Kebieche, Auteur ; Zahia Meraihi, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2009 . - 129 f. ; 30 cm.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biochimie - Microbiologie: Biochimie diabète flavonoïdes Stress oxydant Alloxane Ranunculus repens L. Effet antidiabétique Epirubicine Hépatotoxicité Quercétine Chémoprotecteur stress oxydant flavonoids extract Ranunculus repens L alloxane antidiabetic effect épirubicin Hepatotoxicity Oxidative stress Quercetin Hepatoprotective effect. Index. décimale : 570 Sciences de la vie. Biologie Résumé : In the present study, the antihyperglycemic, anti-diabetic and antioxidant effects of the n-Butanol extract (BE) obtained from air part of Ranunculus repens L plant and quercetin were investigated in normal glucose-fed hyperglycemia, in alloxan induced diabetes and in epirubicin (EPI) toxicity of the rats. The results of this study show antihyperglycemic and antidiabetic activity of the phenolic compounds. And to elucidate a possible mechanism of action of this plant extract, the glycogen liver rate, insulin secretion and the in vitro chelating effect of glucose by plant compounds were also investigated. The flavonoids reduced significantly blood glucose in a dose-dependant manner (200,400,600 mg/kg) in both hyperglycemic and diabetic rats after oral administration as oral hypoglycemic drug, glibenclamide (2.5 mg/kg). We show that flavonoids extracts was able significantly to complex the glucose in vitro, induce insulin secretion of beta cells and to active glycogenesis in liver as shown by the abundance of glycogen granulation in liver histochemical preparation after administration of flavonoids extract. In addition these flavonoids extract and quercetin possess a chemoprotector power against malondialdehyde formation (MDA, lipid peroxidation product) and reduction of antioxidant systems such SOD, CAT and GSH in alloxan-induced diabetic rats. The equilibrium disorder of redox status after alloxan administration is reestablished by flavonoids pretreatment of alloxanised rats. The flavonoids markedly inhibited lipid peroxidation process in pancreatic cells and the proportioning of cytosolic SOD, CAT and GSH showed a significant elevation to establish equilibrium of redox status and consequently protect beta cells and prevent diabetes. The anticancer therapy with EPI produces acute hepatotoxicityby generation of free radicals. However, the oxidative status of the liver cells and mitochondria has been investigated in EPI toxicity of the rats. In this present study, we investigated in first the prooxidant effect of EPI and its implication on both hepatic cells and mitochondria function. EPI injection in rats at dose of 9 mg/kg induced hepatic dysfunction revealed by a significant increase in serum of glutamate oxaloacetate transaminases and glutamate pyruvate transaminases. Oxidative stress in liver cells and mitochondria was provoked by EPI since a statistically significant reduction of CAT, SOD and GSH levels, and significant increase of MDA level, indicator of lipid peroxidation which could perforate the biologic
membranes, were observed. In second, the protective effect of quercetin (33 mg/kg) and flavonoids extract of RRL (100 mg/kg) against oxidative stress induced by EPI was also investigated. Indeed, the pretreatment of rats with flavonoids protected liver cells and mitochondria from oxidative stress permitting, on one hand, the prevention of hepatic dysfunction on maintaining the normal level of serum transaminases following inhibition of their hepatic leakage by preventing lipid peroxidation Thus, the prevention of the cellular membranes perforation, and in the other hand maintaining also antioxidant defense system in mitochondria and cytosol of hepatocytes. Taken together, this data reveal that flavonoids extract of Ranunculus repens L extract and quercetin possess an interesting virtue to be antihyperglycemic and antidiabetic with positive action on liver glycogenesis and insulin secretion. These phenolic compounds have also antiradical and antioxidant power that confers them chemoprotection role against oxidative stress generated in pancreas cells and liver respectively by alloxan and epirubicin.
Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/biologie/KEB5525.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1794 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KEB/5525 KEB/5525 Thèse Bibliothèque principale Thèses Disponible
Titre : Activité comparée de deux produits anabolisants : hormone de croissance et norethandrolone administrés seul ou en association sur la consommation d'oxygène du foie de rat Type de document : texte imprimé Auteurs : Jeanne Naceur, Auteur Editeur : université d'Alger Année de publication : 1972 Importance : 64 f. Note générale : 01 Disponible au magasin de la bibliothèque centrale Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Hormone de croissance oxygène Foie de rat Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Doctorat Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1582 Activité comparée de deux produits anabolisants : hormone de croissance et norethandrolone administrés seul ou en association sur la consommation d'oxygène du foie de rat [texte imprimé] / Jeanne Naceur, Auteur . - université d'Alger, 1972 . - 64 f.
01 Disponible au magasin de la bibliothèque centrale
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Hormone de croissance oxygène Foie de rat Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Doctorat Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1582 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité NAC/021 NAC/021 Thèse Bibliothèque principale Thèses Disponible
Titre : Activité Killer chez des levures isolées des sols du Nord-Est Algérien : Purification, caractérisation et effet sur les souches de levures indésirables. Type de document : texte imprimé Auteurs : Fatima-Zohra-Kenza Labbani, Auteur ; Zahia Meraihi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2015 Importance : 103 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : levures isolement Pichia kluyveri toxine killer biocontrôle levures indésirables Yeast isolation killer toxin biocontrol undesirable yeasts خمائر عزل سم قاتل مكافحة حيوية خمائر غير مرغوب فيها Index. décimale : 570 Sciences de la vie. Biologie Résumé : The general purpose of this thesis is to search for killer activity in yeast strains isolated from agricultural and forest soil in the region of Constantine for biotechnological applications.The isolation of yeasts allows to list 15 strains. The molecular taxonomy based on the sequences of D1/D2 domain of the 26S ribosomal ARN gene groups these isolates into 6 different species: Cryptococcus aerius, Hanseniaspora opuntiae, Hanseniaspora uvarum, Pichia kluyveri, Meyerozyma guilliermondii and Saccharomyces cerevisiae. Morphological, physiological and biochemical characteristics of the strains are in agreement with those cited in
literature. Preliminary testing for killer activity reveals that only the strain L5 is able to produce killer activity. The killer strain is belonging to the species P. kluyveri. The crude killer protein produced by the strain P. kluyveri reveals that the toxin is active against food and beverage spoilage yeast strains belonging to the genera: Dekkera, Kluyveromyces, Pichia, Saccharomyces, Torulaspora, Wickerhamomyces and Zygosaccharomyces. The highest killer activity is obtained against the strain Dekkera bruxellensis DBVPG 6706. Both the heat shock (30 min at 100°C) and the enzymatic treatment with pronase demonstrate the complete loss of the initial killer activity of the toxin, and hence its proteinaceous nature is apparently confirmed. The active protein migrates as a single band in SDS-PAGE and has a molecular mass of 54 kDa. The purified killer toxin has an optimal pH between 4.0-4.5, while the optimal of temperature is 25°C.The determination of minimum inhibitory concentrations (MICs) shows that the purified killer protein has a high in vitro activity against D.bruxellensis (MICs from 64 000- to 256 000-fold lower than that exhibited by potassium metabisulphite) and Saccharomyces cerevisiae (MICs from 32 000- to 64 000-fold lower than potassium sorbate).
No in vitro synergistic interactions (calculated by FIC index -Σ FIC) are observed when killer protein is used in combination with the active compounds : potassium metabisulphite, potassium sorbate, or ethanol. The killer protein exhibited a dose–response effect against D.
bruxellensis and S. cerevisiae in a soft drink « Campari MIXX » (Milan, Italy) and pear juice « Santal » (Parma, Italy), respectively. The results of the present study suggest that the killer protein could be proposed as a novel food-grade compound useful for the control of food and beverage spoilage yeasts. Additionally, the purified killer protein maintains its killing action at
least for 3 days against S. cerevisiae DBVPG 6500 inoculated in pear juice and for 10 days against D. bruxellensis DBVPG 6706 in soft drink. The results of the present study suggest that the killer protein produced by the strain P. kluyveri (L5) could be proposed as a novel natural agent for the biocontrole of beverage spoilage yeasts.
Diplôme : Doctorat En ligne : ../theses/biologie/LAB6794.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10026 Activité Killer chez des levures isolées des sols du Nord-Est Algérien : Purification, caractérisation et effet sur les souches de levures indésirables. [texte imprimé] / Fatima-Zohra-Kenza Labbani, Auteur ; Zahia Meraihi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2015 . - 103 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : levures isolement Pichia kluyveri toxine killer biocontrôle levures indésirables Yeast isolation killer toxin biocontrol undesirable yeasts خمائر عزل سم قاتل مكافحة حيوية خمائر غير مرغوب فيها Index. décimale : 570 Sciences de la vie. Biologie Résumé : The general purpose of this thesis is to search for killer activity in yeast strains isolated from agricultural and forest soil in the region of Constantine for biotechnological applications.The isolation of yeasts allows to list 15 strains. The molecular taxonomy based on the sequences of D1/D2 domain of the 26S ribosomal ARN gene groups these isolates into 6 different species: Cryptococcus aerius, Hanseniaspora opuntiae, Hanseniaspora uvarum, Pichia kluyveri, Meyerozyma guilliermondii and Saccharomyces cerevisiae. Morphological, physiological and biochemical characteristics of the strains are in agreement with those cited in
literature. Preliminary testing for killer activity reveals that only the strain L5 is able to produce killer activity. The killer strain is belonging to the species P. kluyveri. The crude killer protein produced by the strain P. kluyveri reveals that the toxin is active against food and beverage spoilage yeast strains belonging to the genera: Dekkera, Kluyveromyces, Pichia, Saccharomyces, Torulaspora, Wickerhamomyces and Zygosaccharomyces. The highest killer activity is obtained against the strain Dekkera bruxellensis DBVPG 6706. Both the heat shock (30 min at 100°C) and the enzymatic treatment with pronase demonstrate the complete loss of the initial killer activity of the toxin, and hence its proteinaceous nature is apparently confirmed. The active protein migrates as a single band in SDS-PAGE and has a molecular mass of 54 kDa. The purified killer toxin has an optimal pH between 4.0-4.5, while the optimal of temperature is 25°C.The determination of minimum inhibitory concentrations (MICs) shows that the purified killer protein has a high in vitro activity against D.bruxellensis (MICs from 64 000- to 256 000-fold lower than that exhibited by potassium metabisulphite) and Saccharomyces cerevisiae (MICs from 32 000- to 64 000-fold lower than potassium sorbate).
No in vitro synergistic interactions (calculated by FIC index -Σ FIC) are observed when killer protein is used in combination with the active compounds : potassium metabisulphite, potassium sorbate, or ethanol. The killer protein exhibited a dose–response effect against D.
bruxellensis and S. cerevisiae in a soft drink « Campari MIXX » (Milan, Italy) and pear juice « Santal » (Parma, Italy), respectively. The results of the present study suggest that the killer protein could be proposed as a novel food-grade compound useful for the control of food and beverage spoilage yeasts. Additionally, the purified killer protein maintains its killing action at
least for 3 days against S. cerevisiae DBVPG 6500 inoculated in pear juice and for 10 days against D. bruxellensis DBVPG 6706 in soft drink. The results of the present study suggest that the killer protein produced by the strain P. kluyveri (L5) could be proposed as a novel natural agent for the biocontrole of beverage spoilage yeasts.
Diplôme : Doctorat En ligne : ../theses/biologie/LAB6794.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10026 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAB/6794 LAB/6794 Thèse Bibliothèque principale Thèses Disponible
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