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Auteur Z. Meraihi |
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Affiner la recherche Interroger des sources externesCaractérisation d’outils pharmacologiques pour l’étude des récepteurs centraux de la vasopressine / Rafik Marir
Titre : Caractérisation d’outils pharmacologiques pour l’étude des récepteurs centraux de la vasopressine Type de document : texte imprimé Auteurs : Rafik Marir, Auteur ; Z. Meraihi, Directeur de thèse ; Gilles Guillon, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2013 Autre Editeur : Montpellier [France] : Universite Montpellier 1 Importance : 151 f. Format : 30 cm. Note générale : Doctorat 3 ème cycle LMD
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Vasopressin V1A et V1B receptors fluorescent ligands Stress pharmacology vasopressine récepteurs V1a et V1b ligands fluorescents pharmacologie Index. décimale : 570 Sciences de la vie. Biologie Résumé : Neuropeptides(such(as(vasopressin(and(oxytocin,(besides(their(well8known(peripheral(
actions,( regulate( many( cognitive( and( behavioral( functions( via( a( central( action.( Recently,( it(
has( been( shown( that( vasopressin( mediate,( through( V1A,( V1B( and( OT( receptor,( mood,(
learning(and(emotional(disorders(occurring(in(the(limbic(system.(However,(the(V1A(and(V1B(
receptor(sub8types(remain(the(least(studied(because(of(the(lack(of(selective(pharmacological(
tools.(
In(the(rat,(one(of(the(most(used(animals(in(laboratory,(no(high(affinity(agonist(selective(
for(the(V1A(receptor(has(been(characterized(yet.(Similarly(for(V1B,(we(lack(radio8labbel(ou(
fluorescent(tools(to(localize(selectively(this(receptor(in(the(central(nervous(system.((
This(led(us,(in(this(work,(to(focus(on(two(targets(:(
(
V1A$receptor$
This( part( concerns( the( pharmacological( characterization( of( a( new( compound,( the( FE(
201874.( This( compound,( derived( from( the( F8180( was(first(described(as( a( short8acting(
selective(agonist(of(the(human(V1A(receptor.(
Our(results(confirmed(that(FE(201874(is(a(good(ligand(and(V1B/V1A,(V2/V1A(selective(
concerning(binding( experiments( and( OT/V1A( unselective.(FE( 201874( binds( to( the( oxytocin(
receptor(but(with(a(moderate(affinity(and(behaves(as(an(oxytocin(antagonist(in(vitro,(but(not(
in(vivo.(Our(study(has(allowed(the(characterization(of(the(first(selective(V1A(receptor(agonist(
in(rats.(
This( selective( peptide( would( be( particularly( useful( in( developing( new( therapeutic(
strategies(or(pharmacological(studies(aimed(at(better(understanding(of(the(central(roles(of(
these(receptors(in(behavior(and(pathologies.(
(
V1B$receptor$
This( section(concerns( the( characterization( of( the( labeling( and( pharmacological(
properties(of(fluorescent(ligands(derived(from(d([Leu4,(Lys8](VP,(the(first(selective(agonist(of(
the(rat(V1B(receptor,(to(study(the(distribution(of(central(V1B(receptors(in(rats.(
Our(results(showed(that(the(3(fluorescent(ligands(tested(are(good(agonists,(V1A/V1B(
and(V2/V1B(selective(and(OT/V1B(unselective.(Their(ability(to(label(the(V1B(receptors(in(a(
heterologous(cell(culture(system(and(primary(cell(culture(was(also(evaluated,(demonstrating(
that( these( fluorescent( ligands( are( excellent( tools(to( label( and( study( the(V1B( receptors(
localization.( These( ligands( were( then( used( on( fresh( rat( brain( slices( to( study( the( V1B(
receptors(distribution(in(central(regions(involved(in(different(behavioral(processes.(Several(
sites( have( been( identified( including( the( hippocampus,(the(cortex,(the( amygdala,( the(
hypothalamus,(the(olfactory(bulb.(In(addition,(taking(advantage(of(the(agonist(nature(of(the(
fluorescent( ligands,( the( activation( of( the( Map( kinase( pathway( in( living( brain( slices( by( the(
fluorescent( agonists(could(also(be(evidenced( using( a( specific( anti8pERK( antibody.( Thus,(
mapping(the(in#situ(expression(of(V1b(receptors(in(connection(with(their(function(in(normal(
and(stressed(animals(will(become(possible(using(these(tools.(Diplôme : Doctorat En ligne : ../theses/biologie/MAR6392.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9407 Caractérisation d’outils pharmacologiques pour l’étude des récepteurs centraux de la vasopressine [texte imprimé] / Rafik Marir, Auteur ; Z. Meraihi, Directeur de thèse ; Gilles Guillon, Directeur de thèse . - Constantine : Université Mentouri Constantine : Montpellier [France] : Universite Montpellier 1, 2013 . - 151 f. ; 30 cm.
Doctorat 3 ème cycle LMD
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Vasopressin V1A et V1B receptors fluorescent ligands Stress pharmacology vasopressine récepteurs V1a et V1b ligands fluorescents pharmacologie Index. décimale : 570 Sciences de la vie. Biologie Résumé : Neuropeptides(such(as(vasopressin(and(oxytocin,(besides(their(well8known(peripheral(
actions,( regulate( many( cognitive( and( behavioral( functions( via( a( central( action.( Recently,( it(
has( been( shown( that( vasopressin( mediate,( through( V1A,( V1B( and( OT( receptor,( mood,(
learning(and(emotional(disorders(occurring(in(the(limbic(system.(However,(the(V1A(and(V1B(
receptor(sub8types(remain(the(least(studied(because(of(the(lack(of(selective(pharmacological(
tools.(
In(the(rat,(one(of(the(most(used(animals(in(laboratory,(no(high(affinity(agonist(selective(
for(the(V1A(receptor(has(been(characterized(yet.(Similarly(for(V1B,(we(lack(radio8labbel(ou(
fluorescent(tools(to(localize(selectively(this(receptor(in(the(central(nervous(system.((
This(led(us,(in(this(work,(to(focus(on(two(targets(:(
(
V1A$receptor$
This( part( concerns( the( pharmacological( characterization( of( a( new( compound,( the( FE(
201874.( This( compound,( derived( from( the( F8180( was(first(described(as( a( short8acting(
selective(agonist(of(the(human(V1A(receptor.(
Our(results(confirmed(that(FE(201874(is(a(good(ligand(and(V1B/V1A,(V2/V1A(selective(
concerning(binding( experiments( and( OT/V1A( unselective.(FE( 201874( binds( to( the( oxytocin(
receptor(but(with(a(moderate(affinity(and(behaves(as(an(oxytocin(antagonist(in(vitro,(but(not(
in(vivo.(Our(study(has(allowed(the(characterization(of(the(first(selective(V1A(receptor(agonist(
in(rats.(
This( selective( peptide( would( be( particularly( useful( in( developing( new( therapeutic(
strategies(or(pharmacological(studies(aimed(at(better(understanding(of(the(central(roles(of(
these(receptors(in(behavior(and(pathologies.(
(
V1B$receptor$
This( section(concerns( the( characterization( of( the( labeling( and( pharmacological(
properties(of(fluorescent(ligands(derived(from(d([Leu4,(Lys8](VP,(the(first(selective(agonist(of(
the(rat(V1B(receptor,(to(study(the(distribution(of(central(V1B(receptors(in(rats.(
Our(results(showed(that(the(3(fluorescent(ligands(tested(are(good(agonists,(V1A/V1B(
and(V2/V1B(selective(and(OT/V1B(unselective.(Their(ability(to(label(the(V1B(receptors(in(a(
heterologous(cell(culture(system(and(primary(cell(culture(was(also(evaluated,(demonstrating(
that( these( fluorescent( ligands( are( excellent( tools(to( label( and( study( the(V1B( receptors(
localization.( These( ligands( were( then( used( on( fresh( rat( brain( slices( to( study( the( V1B(
receptors(distribution(in(central(regions(involved(in(different(behavioral(processes.(Several(
sites( have( been( identified( including( the( hippocampus,(the(cortex,(the( amygdala,( the(
hypothalamus,(the(olfactory(bulb.(In(addition,(taking(advantage(of(the(agonist(nature(of(the(
fluorescent( ligands,( the( activation( of( the( Map( kinase( pathway( in( living( brain( slices( by( the(
fluorescent( agonists(could(also(be(evidenced( using( a( specific( anti8pERK( antibody.( Thus,(
mapping(the(in#situ(expression(of(V1b(receptors(in(connection(with(their(function(in(normal(
and(stressed(animals(will(become(possible(using(these(tools.(Diplôme : Doctorat En ligne : ../theses/biologie/MAR6392.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9407 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MAR/6392 MAR/6392 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intégraleAdobe Acrobat PDF
Titre : Développement de méthodes bioanalytiques à base d'aptamères pour la determination de l'ochratoxine A Type de document : texte imprimé Auteurs : Amina Rhouati, Auteur ; Z. Meraihi, Directeur de thèse ; Jean-Louis Marty, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2013 Autre Editeur : Université Perpignan Importance : 159 f. Format : 30 cm. Note générale : Doctorat 3ème Cycle LMD
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Mycotoxines OTA aptamères SELEX oligoadsorbant aptacapteur aptamers oligosorbent aptasensors السموم الفطرية إوك ا رتوكسين أبتمير سيميكس عمود امتياز أبتميري مستشع ا رت حيوية أبتيميرية Index. décimale : 570 Sciences de la vie. Biologie Résumé : Ochratoxin A (OTA), an isoucoumarinic mycotoxin produced by the genra Aspergillus and Penicillium, is a common contaminant of several foodstuffs. Even in trace amounts, OTA has adverse affects on human and animal health: nephrotoxic, teratogenic, neurotoxic, immunosuppressive and it was considered by the international Agency of Research on Cancer (IARC) as a potential carcinogenic agent (group 2B). In order to meet food safety concerns and official legislated regulations, analytical methods, mainly based on chromatographic techniques, have been reported for OTA detection. Despite their effectiveness, these
techniques remain expensive and require qualified skilled personnel, alternatives strategies are emerging and novel technologies based on biochemical methods. These techniques are based on the interaction target-recognition element. We use in this work, a new class of biomolecules called aptamers. They are single or double stranded DNA or RNA, selected by their ability to recognise a target, by a combinatorial method of selection in vitro called SELEX (Systematic Evolution of Ligands by EXponantial enrichment).The aim of this work is the development of new methods based on aptamers for OTA determination in foodstuffs.
We will develop two methods, one method for extraction and another one for detection. The first one consists in an aptamer-based affinity column or oligosorbent. This device of extraction was evaluated in term of retention, specificity, selectivity and regeneration. While the second is based on an aptasensor developed in a fully automated system. All the optimizations and manipulations were performed using automated sequences. After optimizations, these two methods are applied for OTA determination in real beer samples.
The obtained limits of detection are much lower than the maximum tolerated levels of OTA in set by the European commission. Also, the high recovery yields obtained show the good applicability of the developed oligosorbent and aptasensor in real sample without matrices effect.Diplôme : Doctorat En ligne : ../theses/biologie/RHO6344.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=6312 Développement de méthodes bioanalytiques à base d'aptamères pour la determination de l'ochratoxine A [texte imprimé] / Amina Rhouati, Auteur ; Z. Meraihi, Directeur de thèse ; Jean-Louis Marty, Directeur de thèse . - constantine [Algérie] : Université Constantine 1 : Université Perpignan, 2013 . - 159 f. ; 30 cm.
Doctorat 3ème Cycle LMD
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Mycotoxines OTA aptamères SELEX oligoadsorbant aptacapteur aptamers oligosorbent aptasensors السموم الفطرية إوك ا رتوكسين أبتمير سيميكس عمود امتياز أبتميري مستشع ا رت حيوية أبتيميرية Index. décimale : 570 Sciences de la vie. Biologie Résumé : Ochratoxin A (OTA), an isoucoumarinic mycotoxin produced by the genra Aspergillus and Penicillium, is a common contaminant of several foodstuffs. Even in trace amounts, OTA has adverse affects on human and animal health: nephrotoxic, teratogenic, neurotoxic, immunosuppressive and it was considered by the international Agency of Research on Cancer (IARC) as a potential carcinogenic agent (group 2B). In order to meet food safety concerns and official legislated regulations, analytical methods, mainly based on chromatographic techniques, have been reported for OTA detection. Despite their effectiveness, these
techniques remain expensive and require qualified skilled personnel, alternatives strategies are emerging and novel technologies based on biochemical methods. These techniques are based on the interaction target-recognition element. We use in this work, a new class of biomolecules called aptamers. They are single or double stranded DNA or RNA, selected by their ability to recognise a target, by a combinatorial method of selection in vitro called SELEX (Systematic Evolution of Ligands by EXponantial enrichment).The aim of this work is the development of new methods based on aptamers for OTA determination in foodstuffs.
We will develop two methods, one method for extraction and another one for detection. The first one consists in an aptamer-based affinity column or oligosorbent. This device of extraction was evaluated in term of retention, specificity, selectivity and regeneration. While the second is based on an aptasensor developed in a fully automated system. All the optimizations and manipulations were performed using automated sequences. After optimizations, these two methods are applied for OTA determination in real beer samples.
The obtained limits of detection are much lower than the maximum tolerated levels of OTA in set by the European commission. Also, the high recovery yields obtained show the good applicability of the developed oligosorbent and aptasensor in real sample without matrices effect.Diplôme : Doctorat En ligne : ../theses/biologie/RHO6344.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=6312 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité RHO/6344 RHO/6344 Thèse Bibliothèque principale Thèses Disponible
Titre : Isolement et caractérisatioin de souches levuriennes amylolytiques à partir de sol saharien algerien Type de document : texte imprimé Auteurs : Ryma Merabti, Auteur ; Z. Meraihi, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2006 Importance : 66 f. Format : 31 cm Note générale : Magister
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Isolement Sol saharien Levures amylolytiques Caractérisation Alpha-amylase Déchets de dattes Thermostabilité Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/MER4504.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1438 Isolement et caractérisatioin de souches levuriennes amylolytiques à partir de sol saharien algerien [texte imprimé] / Ryma Merabti, Auteur ; Z. Meraihi, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2006 . - 66 f. ; 31 cm.
Magister
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Isolement Sol saharien Levures amylolytiques Caractérisation Alpha-amylase Déchets de dattes Thermostabilité Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/MER4504.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1438 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MER/4504 MER/4504 Thèse Bibliothèque principale Thèses Disponible
