| Titre : |
فصل وتحديد بنى نواتج الأيض الثانوي لنبتتين صحراويتين Warionia saharae وPeriploca laevigata. |
| Type de document : |
texte imprimé |
| Auteurs : |
سامية مزهود, Auteur ; رتيبة مكيو, Directeur de thèse |
| Editeur : |
جامعة الإخوة منتوري قسنطينة |
| Année de publication : |
2017 |
| Importance : |
187 ورقة. |
| Format : |
30 سم. |
| Note générale : |
2نسخ موجودة مكتبة المركزية
|
| Langues : |
Arabe (ara) |
| Catégories : |
Arabe
الكيمياء و العلوم ذات العلاقة
|
| Tags : |
الكومارينات الفلافونويدات أحماض الفينولية الفعالية المضادة للأكسدة الزيوت الأساسية (.
Warionia scharae Coss. & Dur. Periploca laevigata Aiton coumarins flavonoïds, phenolic acids antioxidant activity GC-MS essential oil coumarines flavonoïdes acides phénoliques activité antioxydante GC/MS huiles essentielles |
| Index. décimale : |
540 الكيمياء و العلوم ذات العلاقة |
| Résumé : |
This work is devoted to the phytochemical and biological study of two Saharan species used in traditional medicine, the first Warionia saharae (Asteraceae), the second Periploca laevigata belonging to Asclepiadaceae family.
The phytochemical investigation of chloroform, ethyl acetate and n-butanol extracts from the aerial parts of W. saharae allowed the isolation and identification of 13 products belonging to several classes of secondary metabolites (sterols, coumarins, flavonoids and dicaffeoylquinic acid) isolated for the first time from this species excepted one flavonoid (hispidulin). The nbutanol extract of P. laevigata allowed the isolation and identification of 4 flavonoids glycosides. The structural elucidation of the isolated compounds was carried out by combining the various spectroscopic methods (UV, 1D & 2D NMR, ESIMS), acid hydrolysis as well as by comparing the results with the literature data.
The antioxidant activity of the EtOAc extract of W. saharae was evaluated by the free radical
scavenging method (DPPH) and the inhibition of lipid peroxidation (LPO). The EtOAc extract exhibited a strong effect with the IC50 value )6,90 ± 8,0 µg/ml( compared to the standard ) 3, 5 µ 8,21 μg/ml) and the inhibition of lipid peroxidation ) 2 ,9 µ8,6 ± g/ml(. The quantification of the total polyphenols and total flavonoids of the EtOAc and n-BuOH extracts of P. laevigata was performed by colorimetric methods. This step showed a content of polyphenols and flavonoids more important in the n-butanol extract. The antioxidant activity was evaluated by the free radical scavenging method (DPPH). The n-BuOH extract exhibited the strongest effect with the IC50 value 5,9 μg/ml comparatively with ascorbic acid used as standard IC50 (IC50 = 4,65 μg/ml) utilisé comme.
The analysis of essential oil of leaves and flowers of W. saharae by the GC/MS technique, showed a different chemical composition when compared with author works. are described for the first time. De même, the analysis of the essential oil of the aerial parts of P. laevigata by GC/MS, showed a different chemical composition.
|
| Diplôme : |
Doctorat en sciences |
| En ligne : |
../theses/chimie/AMEZ4318.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=10876 |
فصل وتحديد بنى نواتج الأيض الثانوي لنبتتين صحراويتين Warionia saharae وPeriploca laevigata. [texte imprimé] / سامية مزهود, Auteur ; رتيبة مكيو, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 187 ورقة. ; 30 سم. 2نسخ موجودة مكتبة المركزية
Langues : Arabe ( ara)
| Catégories : |
Arabe
الكيمياء و العلوم ذات العلاقة
|
| Tags : |
الكومارينات الفلافونويدات أحماض الفينولية الفعالية المضادة للأكسدة الزيوت الأساسية (.
Warionia scharae Coss. & Dur. Periploca laevigata Aiton coumarins flavonoïds, phenolic acids antioxidant activity GC-MS essential oil coumarines flavonoïdes acides phénoliques activité antioxydante GC/MS huiles essentielles |
| Index. décimale : |
540 الكيمياء و العلوم ذات العلاقة |
| Résumé : |
This work is devoted to the phytochemical and biological study of two Saharan species used in traditional medicine, the first Warionia saharae (Asteraceae), the second Periploca laevigata belonging to Asclepiadaceae family.
The phytochemical investigation of chloroform, ethyl acetate and n-butanol extracts from the aerial parts of W. saharae allowed the isolation and identification of 13 products belonging to several classes of secondary metabolites (sterols, coumarins, flavonoids and dicaffeoylquinic acid) isolated for the first time from this species excepted one flavonoid (hispidulin). The nbutanol extract of P. laevigata allowed the isolation and identification of 4 flavonoids glycosides. The structural elucidation of the isolated compounds was carried out by combining the various spectroscopic methods (UV, 1D & 2D NMR, ESIMS), acid hydrolysis as well as by comparing the results with the literature data.
The antioxidant activity of the EtOAc extract of W. saharae was evaluated by the free radical
scavenging method (DPPH) and the inhibition of lipid peroxidation (LPO). The EtOAc extract exhibited a strong effect with the IC50 value )6,90 ± 8,0 µg/ml( compared to the standard ) 3, 5 µ 8,21 μg/ml) and the inhibition of lipid peroxidation ) 2 ,9 µ8,6 ± g/ml(. The quantification of the total polyphenols and total flavonoids of the EtOAc and n-BuOH extracts of P. laevigata was performed by colorimetric methods. This step showed a content of polyphenols and flavonoids more important in the n-butanol extract. The antioxidant activity was evaluated by the free radical scavenging method (DPPH). The n-BuOH extract exhibited the strongest effect with the IC50 value 5,9 μg/ml comparatively with ascorbic acid used as standard IC50 (IC50 = 4,65 μg/ml) utilisé comme.
The analysis of essential oil of leaves and flowers of W. saharae by the GC/MS technique, showed a different chemical composition when compared with author works. are described for the first time. De même, the analysis of the essential oil of the aerial parts of P. laevigata by GC/MS, showed a different chemical composition.
|
| Diplôme : |
Doctorat en sciences |
| En ligne : |
../theses/chimie/AMEZ4318.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=10876 |
|
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