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Auteur Mesbah Lahouel |
Documents disponibles écrits par cet auteur (3)
Affiner la recherche Interroger des sources externesCaractérisation des souches de Staphylococcus aureus résistantes à la méticilline (SARM) et évaluation de leur sensibilité à la propolis. / Mounira Adoui
Titre : Caractérisation des souches de Staphylococcus aureus résistantes à la méticilline (SARM) et évaluation de leur sensibilité à la propolis. Type de document : texte imprimé Auteurs : Mounira Adoui, Auteur ; Mesbah Lahouel, Directeur de thèse Mention d'édition : 08/04/2019 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 123 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Science biologique :Microbiologie Résistance aux antibiotiques SARM mecA EEP synergie antibiotique propolis Antibiotic resistance MRSA antibiotic-propolis synergy مقاومة المضادات الحيوية SARMالتاثير التآزري للمضادات
الحيويةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Methicillin-resistant Staphylococcus aureus (MRSA) has come to be one of the primary causes of nosocomial and community infections worldwide. The main objective of this thesis was to evaluate the antibacterial activity of propolis ethanol extract EEP with respect to multidrug-resistant bacteria (MRSA). In order to achieve so, clinically isolated MRSA strains had been selected and then studied their resistance profile to different antibiotics families. Methicillin resistance detection has been completed by looking for mecA gene via PCR. The synergy among EEP and 12 anti-staphylococcal antibiotics has also been studied. Antibiotic sensibility study allowed us to detect 34 strains of S. aureus resistant to methicillin MRSA (21.11%) from diverse samples: pus, blood cultures, urine, vaginal samples and
equipment (catheters). The resistance profile analysis of antibiotic MRSA approved the multi-resistant nature of these bacteria to different antibiotics families: aminoglycosides, macrolides and fluoroquinolones. DNA extracts Analysis of 24 MRSA strains by amplification indicated the presence of the mecA gene in 21 strains, showing that this gene was responsible for methicillin resistance in these strains. Antibacterial activity assessment of propolis revealed that EEP exhibited worthy antibacterial activity with respect to all MSSA and MRSA strains. The average MIC and CMB values against the MSSA strains were respectively 0.55 ± 0.45 mg/ml and 5.5 ± 4.5 mg/ml. In the case of MRSA strains, the MIC and CMB were correspondingly: 0.67 ± 0.41 mg/ ml and 6.72 ± 4.16 mg/ml. The addition of EEP at a concentration of 0.25 mg/ml in the medium improved the MRSA strains sensibility to all antibiotics. This analysis showed a synergy between EPP and all tested antibiotics. On the other hand, no case of antagonism between PEF and tested antibiotics was recorded. This reported synergy was relevant, and propolis can be an alternative for these pathogens treatment.Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ADO7565.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11403 Caractérisation des souches de Staphylococcus aureus résistantes à la méticilline (SARM) et évaluation de leur sensibilité à la propolis. [texte imprimé] / Mounira Adoui, Auteur ; Mesbah Lahouel, Directeur de thèse . - 08/04/2019 . - جامعة الإخوة منتوري قسنطينة, 2019 . - 123 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Science biologique :Microbiologie Résistance aux antibiotiques SARM mecA EEP synergie antibiotique propolis Antibiotic resistance MRSA antibiotic-propolis synergy مقاومة المضادات الحيوية SARMالتاثير التآزري للمضادات
الحيويةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Methicillin-resistant Staphylococcus aureus (MRSA) has come to be one of the primary causes of nosocomial and community infections worldwide. The main objective of this thesis was to evaluate the antibacterial activity of propolis ethanol extract EEP with respect to multidrug-resistant bacteria (MRSA). In order to achieve so, clinically isolated MRSA strains had been selected and then studied their resistance profile to different antibiotics families. Methicillin resistance detection has been completed by looking for mecA gene via PCR. The synergy among EEP and 12 anti-staphylococcal antibiotics has also been studied. Antibiotic sensibility study allowed us to detect 34 strains of S. aureus resistant to methicillin MRSA (21.11%) from diverse samples: pus, blood cultures, urine, vaginal samples and
equipment (catheters). The resistance profile analysis of antibiotic MRSA approved the multi-resistant nature of these bacteria to different antibiotics families: aminoglycosides, macrolides and fluoroquinolones. DNA extracts Analysis of 24 MRSA strains by amplification indicated the presence of the mecA gene in 21 strains, showing that this gene was responsible for methicillin resistance in these strains. Antibacterial activity assessment of propolis revealed that EEP exhibited worthy antibacterial activity with respect to all MSSA and MRSA strains. The average MIC and CMB values against the MSSA strains were respectively 0.55 ± 0.45 mg/ml and 5.5 ± 4.5 mg/ml. In the case of MRSA strains, the MIC and CMB were correspondingly: 0.67 ± 0.41 mg/ ml and 6.72 ± 4.16 mg/ml. The addition of EEP at a concentration of 0.25 mg/ml in the medium improved the MRSA strains sensibility to all antibiotics. This analysis showed a synergy between EPP and all tested antibiotics. On the other hand, no case of antagonism between PEF and tested antibiotics was recorded. This reported synergy was relevant, and propolis can be an alternative for these pathogens treatment.Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ADO7565.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11403 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ADO/7565 ADO/7565 Thèse Bibliothèque principale Thèses Disponible
Titre : Effet de la propolis sur les bactéries résistantes aux antibiotiques par inhibition des pompes à efflux. Type de document : texte imprimé Auteurs : Sagia Moussaoui, Auteur ; Mesbah Lahouel, Directeur de thèse Mention d'édition : 02-mai-19 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 64 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Sciences biologiques : Microbiologie Propolis Bactéries Pompes d’efflux inhibition CMIs Infection in vivo Bacteria efflux pumps MICs in vivo infection العكبر بكتيريا مضخات التدفق تثبيط التركيز الأدنى المثبط عدوى بكتيرية Index. décimale : 570 Sciences de la vie. Biologie Résumé : The resistance of bacteria to various anti-infectious chemotherapies has become a real public health problem. Bacteria ovoid antibiotics through the development of antimicrobiol resistance by draining grugs out the cells. Drug efflux is a key mechanism of resistance mechanism using pump systems located on the surface of bacterial membranes. For this, genetically modified bacteria carrying different types of efflux pumps were chosen as the study model on which Algerian propolis was tested. The tests in vitro performed on different bacterial strains have shown results comparable to those reported in the literature and confirm that Gram-positive bacteria are more sensitive to the effect of propolis than Gram-negative bacteria. In addition, the effectiveness of propolis also extends to resistant strains by efflux pump mechanisms The satisfactory results obtained encouraged us to carry out in vivo studies on wistar rats. The effects on a model of induced infection in rats were studied and evaluated by different laboratory parameters. Restoring the parameters tested in vivo after treatment of the infection with propolis, could confirm the interest of using it as a natural agent capable of countering the effects of oxidative stress linked to infections such as sepsis. As few information, on the clinical efficacy of Algerian propolis, is available, for this, a new step would be interesting to complete the research and thus develop the clinical investigations obtained which would make it possible to evaluate the beneficial effect of propolis in patients. Also the results obtained in this modest work could be used to justify the place that Algerian propolis may have for alternative therapies in the search. Note de contenu : Annexe. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MOU7694.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11528 Effet de la propolis sur les bactéries résistantes aux antibiotiques par inhibition des pompes à efflux. [texte imprimé] / Sagia Moussaoui, Auteur ; Mesbah Lahouel, Directeur de thèse . - 02-mai-19 . - جامعة الإخوة منتوري قسنطينة, 2019 . - 64 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Sciences biologiques : Microbiologie Propolis Bactéries Pompes d’efflux inhibition CMIs Infection in vivo Bacteria efflux pumps MICs in vivo infection العكبر بكتيريا مضخات التدفق تثبيط التركيز الأدنى المثبط عدوى بكتيرية Index. décimale : 570 Sciences de la vie. Biologie Résumé : The resistance of bacteria to various anti-infectious chemotherapies has become a real public health problem. Bacteria ovoid antibiotics through the development of antimicrobiol resistance by draining grugs out the cells. Drug efflux is a key mechanism of resistance mechanism using pump systems located on the surface of bacterial membranes. For this, genetically modified bacteria carrying different types of efflux pumps were chosen as the study model on which Algerian propolis was tested. The tests in vitro performed on different bacterial strains have shown results comparable to those reported in the literature and confirm that Gram-positive bacteria are more sensitive to the effect of propolis than Gram-negative bacteria. In addition, the effectiveness of propolis also extends to resistant strains by efflux pump mechanisms The satisfactory results obtained encouraged us to carry out in vivo studies on wistar rats. The effects on a model of induced infection in rats were studied and evaluated by different laboratory parameters. Restoring the parameters tested in vivo after treatment of the infection with propolis, could confirm the interest of using it as a natural agent capable of countering the effects of oxidative stress linked to infections such as sepsis. As few information, on the clinical efficacy of Algerian propolis, is available, for this, a new step would be interesting to complete the research and thus develop the clinical investigations obtained which would make it possible to evaluate the beneficial effect of propolis in patients. Also the results obtained in this modest work could be used to justify the place that Algerian propolis may have for alternative therapies in the search. Note de contenu : Annexe. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MOU7694.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11528 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MOU/7694 MOU/7694 Thèse Bibliothèque principale Thèses Disponible
Titre : Modulation of the apoptotic effects of terpenes by acting on the mitochondrial Enzymatic system in hepatic normal and Cancer cells. Type de document : texte imprimé Auteurs : Malika Boulmeltout, Auteur ; Mesbah Lahouel, Directeur de thèse Mention d'édition : 02/05/2019 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 164 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biochimie et Biologie Cellulaire et Moléculaire: Toxicology Bl6 F1melanoma FAO hepatocytes benzol (a) pyrène cancer du stress oxydatif ferulenol coladine lapiférine Ferulavesceritensis FAO Bl6F1 melanoma benzo(a)pyrane liver cancer oxidative stress ferulenolcoladin lapiferin خلایا melanoma1FAOhepatocytes, B16Fالإجھاد التأكسدي بنزوبیران سرطان الكبد فیریلینول ین
Ferulavesceritensis. كولاد لا,بیرفیرینIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Ferula vesceritensis roots are potential sources of natural antioxidant and prooxidant esquiterpenecoumarinsthat could potentially have antiproliferative, cytotoxic effects against many types of cancers including liver cancer which is the principle cause of death worldwide. The aim of this work is to investigate the biological activity of sesquiterpenecoumarins, ferulenol,coladin and lepiferin obtained from the Ferula roots crude methanol extract. These bioactive compounds whose structures determined by extension NMR analysis are explored in vivo and in vitro studies by following the prooxidant effects, tested on
experimental liver cancer induced by Benzo(a)pyrane,and cytotoxic effects on B16F1 melanoma and FAO cell lines. The impact of these bioactive compounds over the mitochondrial function has also been evaluated in liver mitochondria. Ferulenol, coladin and lapiferin have increased the oxidative stress by variably decreasing the enzymatic and non enzymatic antioxidants (SOD CAT, GST) and increasing the lipid peroxidation as well as diminishing the mitochondrial dehydrogenases activities tested on Bl6F1 melanoma and FAO cells with MTT and WST-1 assay after 24 and 48 hrs throughout an apoptotic pathway.At the level of mitochondria, those bioactive substances have caused mitochondrial swelling and a variable diminution in the mitochondrial superoxide anion O-2productionin isolated mitochondrial liver in a dose dependant manner. The prooxidant, cytotoxic and/or chemopreventiveeffects of Ferulavesceritensis roots sesquiterpenecoumarins have permitted us to suggest the possible potential exploration of those bioactive compounds for combating and/or preventing many diseases such as liver cancer.
Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BOU7583.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11420 Modulation of the apoptotic effects of terpenes by acting on the mitochondrial Enzymatic system in hepatic normal and Cancer cells. [texte imprimé] / Malika Boulmeltout, Auteur ; Mesbah Lahouel, Directeur de thèse . - 02/05/2019 . - جامعة الإخوة منتوري قسنطينة, 2019 . - 164 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biochimie et Biologie Cellulaire et Moléculaire: Toxicology Bl6 F1melanoma FAO hepatocytes benzol (a) pyrène cancer du stress oxydatif ferulenol coladine lapiférine Ferulavesceritensis FAO Bl6F1 melanoma benzo(a)pyrane liver cancer oxidative stress ferulenolcoladin lapiferin خلایا melanoma1FAOhepatocytes, B16Fالإجھاد التأكسدي بنزوبیران سرطان الكبد فیریلینول ین
Ferulavesceritensis. كولاد لا,بیرفیرینIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Ferula vesceritensis roots are potential sources of natural antioxidant and prooxidant esquiterpenecoumarinsthat could potentially have antiproliferative, cytotoxic effects against many types of cancers including liver cancer which is the principle cause of death worldwide. The aim of this work is to investigate the biological activity of sesquiterpenecoumarins, ferulenol,coladin and lepiferin obtained from the Ferula roots crude methanol extract. These bioactive compounds whose structures determined by extension NMR analysis are explored in vivo and in vitro studies by following the prooxidant effects, tested on
experimental liver cancer induced by Benzo(a)pyrane,and cytotoxic effects on B16F1 melanoma and FAO cell lines. The impact of these bioactive compounds over the mitochondrial function has also been evaluated in liver mitochondria. Ferulenol, coladin and lapiferin have increased the oxidative stress by variably decreasing the enzymatic and non enzymatic antioxidants (SOD CAT, GST) and increasing the lipid peroxidation as well as diminishing the mitochondrial dehydrogenases activities tested on Bl6F1 melanoma and FAO cells with MTT and WST-1 assay after 24 and 48 hrs throughout an apoptotic pathway.At the level of mitochondria, those bioactive substances have caused mitochondrial swelling and a variable diminution in the mitochondrial superoxide anion O-2productionin isolated mitochondrial liver in a dose dependant manner. The prooxidant, cytotoxic and/or chemopreventiveeffects of Ferulavesceritensis roots sesquiterpenecoumarins have permitted us to suggest the possible potential exploration of those bioactive compounds for combating and/or preventing many diseases such as liver cancer.
Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BOU7583.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11420 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/7583 BOU/7583 Thèse Bibliothèque principale Thèses Disponible
