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Auteur Ilhem Mihoubi-Djezzare |
Documents disponibles écrits par cet auteur (8)
Affiner la recherche Interroger des sources externesCaractérisation moléculaire et par MALDI-TOF MS des espèces fongiques phytopathogènes inféodées aux céréales et mise en évidence de l’impact de l’utilisation des antifongiques azolés sur la résistance des souches. / Ahlem Zaamouchi
Titre : Caractérisation moléculaire et par MALDI-TOF MS des espèces fongiques phytopathogènes inféodées aux céréales et mise en évidence de l’impact de l’utilisation des antifongiques azolés sur la résistance des souches. Type de document : texte imprimé Auteurs : Ahlem Zaamouchi, Auteur ; Ilhem Mihoubi-Djezzare, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 139 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Microbiologie: Bioprocédés et Biotechnologie, Applications Mycologiques Blé dur Fongicides triazolés MALDI-TOF MS Résistance CYP 51A Durum wheat Triazole fungicides Resistance cyp51A الجين cyp51Aالقمح الصلب مبيدات فطرية التريازولات المقاومة مصفوفة المساعدة بالليزر و الامتزاز التايني في وقت الرحلة الكتلية الطيفية Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Winter cereals including wheat remain the staple food of the Algerian diet and show a strategic importance in both human and animal nutrition. Many fungal diseases may attack wheat farmings, These attacks may cause great loss when the used varieties are sensitive. The mildews are phytopathogenic But, they are eventually responsible of human pathologies. The treatment protects the plant from every external contamination. The best phytosanitary protection of wheat farmingsis assured via two types of treatments; fungicidal and insecticide. The fungicides if used to destroy pathogenic fungi which attach the farmings, seeds and harvested products. Currently, the mildews are becoming resistant in the environment, because of the exercised pressure through the use of fungicides. The most common mildew’s resistance mechanism is the mutation of the cyp51 gene coding 14alpha-Demethylase sterol. The azolated antifungals inhibit this enzyme, leading to blocking the biosynthesis of the ergosterol which leads to deplete the ergosterol And to an accumulation of toxic metabolites. In this context, we have conducted a comparative study both seed samples, leaves and wheat spikes: one of them is treated by the azolated fungicide of type: Raxil® 060 FS, l’Artea330EC and Amistar® Xtra while the other is kept untreated in order to evaluate the
efficiency of triazoles and the degree of protection of the wheat farming after a fungicidal treatment in the province of Constantine (Algeria). According to the mycological analysis of the samples, 114 strains have been isolated; the species of Aspergillus and Penicillium genus are the most dominant. 6 species resistant against the treatments were selected for the study of fungicidal effects including (A. flavus, A. tubingensis, P. glabrum, P.griseofulvum, T. harzianium and A. alternate). The study of the sensitivity of these isolates covers firstly the evaluation of the in vitro activity of the pre-described fungicides via a diffusion technique. In addition, the obtained results validated with a statistical analysis revealed that the tested fungicides caused a decrease in the pycnidial Coating compared to control samples. Secondly, the detection of resistance mutations in the isolate’s cyp51 gene was accomplished through the analysis of this latter. The obtained results were treated using a bioinformatics approach in order to exploit them.
Note de contenu :
Annexes.
Diplôme : Doctorat En ligne : ../theses/biologie/ZAA7453.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11238 Caractérisation moléculaire et par MALDI-TOF MS des espèces fongiques phytopathogènes inféodées aux céréales et mise en évidence de l’impact de l’utilisation des antifongiques azolés sur la résistance des souches. [texte imprimé] / Ahlem Zaamouchi, Auteur ; Ilhem Mihoubi-Djezzare, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2019 . - 139 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Microbiologie: Bioprocédés et Biotechnologie, Applications Mycologiques Blé dur Fongicides triazolés MALDI-TOF MS Résistance CYP 51A Durum wheat Triazole fungicides Resistance cyp51A الجين cyp51Aالقمح الصلب مبيدات فطرية التريازولات المقاومة مصفوفة المساعدة بالليزر و الامتزاز التايني في وقت الرحلة الكتلية الطيفية Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Winter cereals including wheat remain the staple food of the Algerian diet and show a strategic importance in both human and animal nutrition. Many fungal diseases may attack wheat farmings, These attacks may cause great loss when the used varieties are sensitive. The mildews are phytopathogenic But, they are eventually responsible of human pathologies. The treatment protects the plant from every external contamination. The best phytosanitary protection of wheat farmingsis assured via two types of treatments; fungicidal and insecticide. The fungicides if used to destroy pathogenic fungi which attach the farmings, seeds and harvested products. Currently, the mildews are becoming resistant in the environment, because of the exercised pressure through the use of fungicides. The most common mildew’s resistance mechanism is the mutation of the cyp51 gene coding 14alpha-Demethylase sterol. The azolated antifungals inhibit this enzyme, leading to blocking the biosynthesis of the ergosterol which leads to deplete the ergosterol And to an accumulation of toxic metabolites. In this context, we have conducted a comparative study both seed samples, leaves and wheat spikes: one of them is treated by the azolated fungicide of type: Raxil® 060 FS, l’Artea330EC and Amistar® Xtra while the other is kept untreated in order to evaluate the
efficiency of triazoles and the degree of protection of the wheat farming after a fungicidal treatment in the province of Constantine (Algeria). According to the mycological analysis of the samples, 114 strains have been isolated; the species of Aspergillus and Penicillium genus are the most dominant. 6 species resistant against the treatments were selected for the study of fungicidal effects including (A. flavus, A. tubingensis, P. glabrum, P.griseofulvum, T. harzianium and A. alternate). The study of the sensitivity of these isolates covers firstly the evaluation of the in vitro activity of the pre-described fungicides via a diffusion technique. In addition, the obtained results validated with a statistical analysis revealed that the tested fungicides caused a decrease in the pycnidial Coating compared to control samples. Secondly, the detection of resistance mutations in the isolate’s cyp51 gene was accomplished through the analysis of this latter. The obtained results were treated using a bioinformatics approach in order to exploit them.
Note de contenu :
Annexes.
Diplôme : Doctorat En ligne : ../theses/biologie/ZAA7453.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11238 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ZAA/7453 ZAA/7453 Thèse Bibliothèque principale Thèses Disponible
Titre : Detection Moleculaire Des Leishmanies A Partir Du Genre Phlebotomus (Diptera : Psychodidae) : tendance vers la regression de la leishmaniose a Constantine ? Type de document : texte imprimé Auteurs : Khalida Frahtia-Benotmane, Auteur ; Ilhem Mihoubi-Djezzare, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2015 Importance : 130 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Phlebotomus Détection moléculaire PCR en temps réel Constantine Molecular diagnostic Real time PCR - Constantine.
لشمانيا فميبوطوم التشخيص الجزيئي PCR في الوقت الحقيقي قسنطينةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Leishmaniasis is a group of parasitic disease with a varied clinical expression caused by flagellate protozoa of the Leishmania genus. These diseases are transmitted to humans and animals by the sting of a vector insect, the female sandfly. Among the groups of dipteral disease vectors, Phlebotominae occupy a prime position and play a significant role in human pathology, such as leishmaniasis that affects nearly 350 million people worldwide. The vector control operation launched by health services throughout the country proves to be effective since despite the prevalence of the disease remains high especially in rural areas, leishmaniasis appears to be declining in Algeria. In this context, this study mainly concerns molecular detection of Leishmania from the vector. Furthermore, a molecular diagnosis has also been made on skin samples taken from patients in the region of Constantine. Concerning the vector, 5858 sandflies were captured, including 4360 males and 1498 females. Male
specimens were identified based on their morphological. The morphological identification highlighted the presence of the Phlebotomus genus with a prevalence of 93% against 7% represented by the Sergentomyia genus. About the identified species, P.perniciosus is the most abundant with 59.4% of the male identified population followed by P.longicuspis with 24.7% of the workforce. P.perfiliewi is poorly represented by 6.7% of specimens followed by
P.papatasi with 2.2% and 1.5% S.dreyfussi. Concerning skin samples, 45/79 (56.96%) collected samples were found positive by real-time PCR. This rate appears to be in sharp decline compared to previous years (alert peak of 30,227 cases in 2005). Concerning the detection of Leishmania from sandflies by RT-PCR, the results show that 3/60 PCR performed genus are positive with melting temperatures corresponding to that of the reference strain (84.1 +/- 0.4 ° C for L. infantum). This proves that the vectors were parasitized. On the other side, identification by RT-PCR species didn't give any results. This could be explained by the presence of an insufficient amount of leishmanian DNA in the vector, and therefore support the hypothesis of the regression of leishmaniasis in Constantine.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/FRA6762.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9851 Detection Moleculaire Des Leishmanies A Partir Du Genre Phlebotomus (Diptera : Psychodidae) : tendance vers la regression de la leishmaniose a Constantine ? [texte imprimé] / Khalida Frahtia-Benotmane, Auteur ; Ilhem Mihoubi-Djezzare, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2015 . - 130 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Phlebotomus Détection moléculaire PCR en temps réel Constantine Molecular diagnostic Real time PCR - Constantine.
لشمانيا فميبوطوم التشخيص الجزيئي PCR في الوقت الحقيقي قسنطينةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Leishmaniasis is a group of parasitic disease with a varied clinical expression caused by flagellate protozoa of the Leishmania genus. These diseases are transmitted to humans and animals by the sting of a vector insect, the female sandfly. Among the groups of dipteral disease vectors, Phlebotominae occupy a prime position and play a significant role in human pathology, such as leishmaniasis that affects nearly 350 million people worldwide. The vector control operation launched by health services throughout the country proves to be effective since despite the prevalence of the disease remains high especially in rural areas, leishmaniasis appears to be declining in Algeria. In this context, this study mainly concerns molecular detection of Leishmania from the vector. Furthermore, a molecular diagnosis has also been made on skin samples taken from patients in the region of Constantine. Concerning the vector, 5858 sandflies were captured, including 4360 males and 1498 females. Male
specimens were identified based on their morphological. The morphological identification highlighted the presence of the Phlebotomus genus with a prevalence of 93% against 7% represented by the Sergentomyia genus. About the identified species, P.perniciosus is the most abundant with 59.4% of the male identified population followed by P.longicuspis with 24.7% of the workforce. P.perfiliewi is poorly represented by 6.7% of specimens followed by
P.papatasi with 2.2% and 1.5% S.dreyfussi. Concerning skin samples, 45/79 (56.96%) collected samples were found positive by real-time PCR. This rate appears to be in sharp decline compared to previous years (alert peak of 30,227 cases in 2005). Concerning the detection of Leishmania from sandflies by RT-PCR, the results show that 3/60 PCR performed genus are positive with melting temperatures corresponding to that of the reference strain (84.1 +/- 0.4 ° C for L. infantum). This proves that the vectors were parasitized. On the other side, identification by RT-PCR species didn't give any results. This could be explained by the presence of an insufficient amount of leishmanian DNA in the vector, and therefore support the hypothesis of the regression of leishmaniasis in Constantine.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/FRA6762.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9851 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité FRA/6762 FRA/6762 Thèse Bibliothèque principale Thèses Disponible
Titre : Elément d'analyse de différents composants de la microflore digestive et fecale de dromadaires (camelus dromedarius) élevés en milieu naturel Type de document : texte imprimé Auteurs : Ilhem Mihoubi-Djezzare ; H. Bousseboua, Directeur de thèse ; Univ. de Constantine, Éditeur scientifique Année de publication : 1994 Importance : 42 f. Note générale : 1 Disponible à la salle de recherche
2 Disponibles au magasin de la bibliothèque centraleLangues : Français (fre) Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Dromadaire Microflore digestive Index. décimale : 640 Industrie Alimentaire Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=4478 Elément d'analyse de différents composants de la microflore digestive et fecale de dromadaires (camelus dromedarius) élevés en milieu naturel [texte imprimé] / Ilhem Mihoubi-Djezzare ; H. Bousseboua, Directeur de thèse ; Univ. de Constantine, Éditeur scientifique . - 1994 . - 42 f.
1 Disponible à la salle de recherche
2 Disponibles au magasin de la bibliothèque centrale
Langues : Français (fre)
Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Dromadaire Microflore digestive Index. décimale : 640 Industrie Alimentaire Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=4478 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MIH/2687 MIH/2687 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude de la biodégradation de l’ortho-nitrophénol et du para-nitrophénol par le microbiote des eaux usées et mise en évidence des bactéries dominantes impliquées dans ce processus Type de document : texte imprimé Auteurs : Amel Daffri, Auteur ; Ilhem Mihoubi-Djezzare, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 116 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles.
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biodégradation PNP ONP Microbiote Eaux usées Pseudomonas aeruginosa Bacillus cereus Biodegradation Microflora Wastewater نيتورفينول -pنيتروفينول الھدم الحيوي ميكروفلورا المياه المستعملة Index. décimale : 570 Sciences de la vie. Biologie Résumé : Biodegradation of ortho-Nitrophenol (ONP) and para-Nitrophenol (PNP) by wastewater’s microflora is studied. Isolation and identification of microorganisms implicated is also carried out. The cultures are performed using a minimum medium, where Nitrophenol is the sole source of carbon, energy and nitrogen at 500 mg/L. The kinetic of biodegradation is followed for more than 30 days of incubation. The obtained results show that more than
90% of initial concentration of PNP is decomposed at the end of 30 days of incubation and 77% of ONP in the same time of incubation. HPLC and GC-MS analysis permit to identify products and pathway's biodegradation of the two isomers. The isolation of microorganisms degrading PNP gave four bacterial colonies of different macroscopic aspects. However, three types of these bacterial are pigmented. Sequence analysis of 16S ribosomal RNA indicated
that the ONP and the PNP degrading isolates were closely related to members of the species: Pseudomonas aeruginosa and Bacillus cereus.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/DAF7170.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10587 Etude de la biodégradation de l’ortho-nitrophénol et du para-nitrophénol par le microbiote des eaux usées et mise en évidence des bactéries dominantes impliquées dans ce processus [texte imprimé] / Amel Daffri, Auteur ; Ilhem Mihoubi-Djezzare, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 116 f. ; 30 cm.
2 copies imprimées disponibles.
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biodégradation PNP ONP Microbiote Eaux usées Pseudomonas aeruginosa Bacillus cereus Biodegradation Microflora Wastewater نيتورفينول -pنيتروفينول الھدم الحيوي ميكروفلورا المياه المستعملة Index. décimale : 570 Sciences de la vie. Biologie Résumé : Biodegradation of ortho-Nitrophenol (ONP) and para-Nitrophenol (PNP) by wastewater’s microflora is studied. Isolation and identification of microorganisms implicated is also carried out. The cultures are performed using a minimum medium, where Nitrophenol is the sole source of carbon, energy and nitrogen at 500 mg/L. The kinetic of biodegradation is followed for more than 30 days of incubation. The obtained results show that more than
90% of initial concentration of PNP is decomposed at the end of 30 days of incubation and 77% of ONP in the same time of incubation. HPLC and GC-MS analysis permit to identify products and pathway's biodegradation of the two isomers. The isolation of microorganisms degrading PNP gave four bacterial colonies of different macroscopic aspects. However, three types of these bacterial are pigmented. Sequence analysis of 16S ribosomal RNA indicated
that the ONP and the PNP degrading isolates were closely related to members of the species: Pseudomonas aeruginosa and Bacillus cereus.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/DAF7170.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10587 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité DAF/7170 DAF/7170 Thèse Bibliothèque principale Thèses Disponible 
Titre : Etude, in vitro, de l’activite anti leishmanienne de certaines plantes medicinales locales : cas de la famille des lamiacees Type de document : texte imprimé Auteurs : Imene Ramli, Auteur ; Ilhem Mihoubi-Djezzare, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2013 Importance : 106 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Leishmaniose Activité anti-leishmanienne Salvia aurasiaca Stachys guyoniana Lamiaceae Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Magistère En ligne : ../theses/biologie/RAM6382.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9383 Etude, in vitro, de l’activite anti leishmanienne de certaines plantes medicinales locales : cas de la famille des lamiacees [texte imprimé] / Imene Ramli, Auteur ; Ilhem Mihoubi-Djezzare, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2013 . - 106 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Leishmaniose Activité anti-leishmanienne Salvia aurasiaca Stachys guyoniana Lamiaceae Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Magistère En ligne : ../theses/biologie/RAM6382.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9383 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité RAM/6382 RAM/6382 Thèse Bibliothèque principale Thèses Disponible Documents numériques
RAM6382.pdfAdobe Acrobat PDF
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