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Partager le résultat de cette recherche Interroger des sources externesExtraction, purification, caractérisation et effet immuno-modulateur Des lectines fongiques de Terfèzia boudiéri (Truffe Blanche du Sahara) / Abdelbaki Zitouni
Titre : Extraction, purification, caractérisation et effet immuno-modulateur Des lectines fongiques de Terfèzia boudiéri (Truffe Blanche du Sahara) Type de document : texte imprimé Auteurs : Abdelbaki Zitouni, Auteur ; Youcef Necib, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2015 Importance : 140 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Lectines Extraction Purification Agglutination Activité phagocytaire Lectins Activity Phagocytic Cells ??????? ????? ????????? ???? ???? ??????? ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Lectins are ubiquitous, polyvalent and non immune origin which bind specifically and reversibly to carbohydrates. They are often multivalent and have the well-known ability to agglutinate erythrocytes.
Fungi, including microscopic fungi and yeasts, have been the subject of considerable interest in recent years because of their potential biomedical and biotechnological applications. The information on fungal lectins, especially the structural point of view, are minimal compared to those of their plant and animal counterparts. It is in this context that lies our purpose was realized by the biochemical and structural characterization of fungal lectin Terfezia boudieri ( Algerian Sahara Truffle) on which we previously had no such data.
Two lectins: TrFBL1 and TrFBL2 were extracted, purified, characterized and sequened. The structures of 3D TrFBL1 native protein and its complex with a ligand, fucoside sélénométhyl were obtained and are being refined .The structures of TrFBL2 complexes with N-acetylglucosamine and two other glycans were obtained at high resolution.
The Genome of Terfezia boudieri was obtained and is available from the month of December 2013 and will become part of the database collected Lectin 3D (http://lectin3d.cermav.cnrs.fr).
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ZIT6699.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9924 Extraction, purification, caractérisation et effet immuno-modulateur Des lectines fongiques de Terfèzia boudiéri (Truffe Blanche du Sahara) [texte imprimé] / Abdelbaki Zitouni, Auteur ; Youcef Necib, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2015 . - 140 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Lectines Extraction Purification Agglutination Activité phagocytaire Lectins Activity Phagocytic Cells ??????? ????? ????????? ???? ???? ??????? ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Lectins are ubiquitous, polyvalent and non immune origin which bind specifically and reversibly to carbohydrates. They are often multivalent and have the well-known ability to agglutinate erythrocytes.
Fungi, including microscopic fungi and yeasts, have been the subject of considerable interest in recent years because of their potential biomedical and biotechnological applications. The information on fungal lectins, especially the structural point of view, are minimal compared to those of their plant and animal counterparts. It is in this context that lies our purpose was realized by the biochemical and structural characterization of fungal lectin Terfezia boudieri ( Algerian Sahara Truffle) on which we previously had no such data.
Two lectins: TrFBL1 and TrFBL2 were extracted, purified, characterized and sequened. The structures of 3D TrFBL1 native protein and its complex with a ligand, fucoside sélénométhyl were obtained and are being refined .The structures of TrFBL2 complexes with N-acetylglucosamine and two other glycans were obtained at high resolution.
The Genome of Terfezia boudieri was obtained and is available from the month of December 2013 and will become part of the database collected Lectin 3D (http://lectin3d.cermav.cnrs.fr).
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ZIT6699.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9924 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ZIT/6699 ZIT/6699 Thèse Bibliothèque principale Thèses Disponible
Titre : Extraction, purification et caractérisation des lectines produites par la souche Micromonospora aurantiaca GF44c, et tests biologiques Type de document : texte imprimé Auteurs : Fateh Merouane, Auteur ; Youcef Necib, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 155 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Microbiologie Biotechnologies Microbiennes, Génomes et Environnement Actinomycètes Micromonospora aurantiaca Lectines Vésicules membranaires Protéomique Lectins Membrane vesicles Proteomic ??????????? ????????? ????????? ???????? ??????? ?????????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Lectins are ubiquitous proteins, non-immune origin, presenting several biological
properties, due to their unique ability to read the biological information codified in the threedimensional structure of sugars and glycoproteins. In this work, 6 strains of actinomycetes, which 5 belonging to rare genus were tested for their production of lectins. The hemagglutination test was used to select the strain Micromonospora aurantiaca GF 44c as best producing strain of these molecules. The study of the lectins contained in the intracellular fraction of strain Micromonospora aurantiaca GF44c led to the discovery of membrane vesicles of nanometric size similar to those found in other Gram-positive bacteria (30 to
100nm) through the use of different technical (FPLC Purification on Superose 6, transmission electron microscopy and dynamic light scattering). These vesicles have the ability to induce, in addition to hemagglutination, immunomodulatory and proteolytic activities. The SDSPAGE shows that these vesicles are very rich in diverse proteins. The proteomic analysis by mass spectrometry of these membrane vesicles identified 81 different proteins, mainly adhesins and membrane transporters (Substrate binding proteins and ABC transporters), enzymes (metalloprotease, peptidase, succinate dehydrogenase, Prolipoprotein diacylglyceryl transferase, DCSA oxidoreductase, Glutamine synthetase, N-acetylmuramoyl-L-alanine amidase), and lipoproteins. Functional analysis of these vesicular protein, suggest that these
vesicles play an important role in the survival of the bacterium in a stressful particular biological environment, by the nutrient acquisition (amino acids carbohydrates and inorganic ions), the resistance to stress factors through the action of several enzymes, and redirect the immune system of the host by a strong immunostimulation. This study describes for the first time the production of membrane vesicles in the genus Micromonospora, and the possible roles of these vesicles.Diplôme : Doctorat En ligne : ../theses/biologie/MER6884.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10205 Extraction, purification et caractérisation des lectines produites par la souche Micromonospora aurantiaca GF44c, et tests biologiques [texte imprimé] / Fateh Merouane, Auteur ; Youcef Necib, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2016 . - 155 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Microbiologie Biotechnologies Microbiennes, Génomes et Environnement Actinomycètes Micromonospora aurantiaca Lectines Vésicules membranaires Protéomique Lectins Membrane vesicles Proteomic ??????????? ????????? ????????? ???????? ??????? ?????????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Lectins are ubiquitous proteins, non-immune origin, presenting several biological
properties, due to their unique ability to read the biological information codified in the threedimensional structure of sugars and glycoproteins. In this work, 6 strains of actinomycetes, which 5 belonging to rare genus were tested for their production of lectins. The hemagglutination test was used to select the strain Micromonospora aurantiaca GF 44c as best producing strain of these molecules. The study of the lectins contained in the intracellular fraction of strain Micromonospora aurantiaca GF44c led to the discovery of membrane vesicles of nanometric size similar to those found in other Gram-positive bacteria (30 to
100nm) through the use of different technical (FPLC Purification on Superose 6, transmission electron microscopy and dynamic light scattering). These vesicles have the ability to induce, in addition to hemagglutination, immunomodulatory and proteolytic activities. The SDSPAGE shows that these vesicles are very rich in diverse proteins. The proteomic analysis by mass spectrometry of these membrane vesicles identified 81 different proteins, mainly adhesins and membrane transporters (Substrate binding proteins and ABC transporters), enzymes (metalloprotease, peptidase, succinate dehydrogenase, Prolipoprotein diacylglyceryl transferase, DCSA oxidoreductase, Glutamine synthetase, N-acetylmuramoyl-L-alanine amidase), and lipoproteins. Functional analysis of these vesicular protein, suggest that these
vesicles play an important role in the survival of the bacterium in a stressful particular biological environment, by the nutrient acquisition (amino acids carbohydrates and inorganic ions), the resistance to stress factors through the action of several enzymes, and redirect the immune system of the host by a strong immunostimulation. This study describes for the first time the production of membrane vesicles in the genus Micromonospora, and the possible roles of these vesicles.Diplôme : Doctorat En ligne : ../theses/biologie/MER6884.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10205 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MER/6884 MER/6884 Thèse Bibliothèque principale Thèses Disponible
Titre : Potentiel antioxydant des algues des côtes de l’est Algérien. Type de document : texte imprimé Auteurs : Hichem Mezdour, Auteur ; Ahmed Menad, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 119 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : U. lactuca polysaccharides lectins anti-inflammatoire immune-modulatoire antioxydant anti-inflammatory immuno-modulatory antioxidant ????? ??????? ???? ???????? ????????? ???? ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The number of studies of marine algae extracts from the Algerian species Ulva lactuca is so little. This study aims to examine the biological potential of polyphenoles, polysaccharide and lectins fractions of the species U. lactuca collected from eastern Algeria (Jijel). The polyphenoles fractions was evaluated by testing the antioxidant activity in vitro, the carrageenin-induced anti-inflammatory activity in the Albino wistar rat, and the immunomodulatory effect in vivo by assessing the index of phagocytosis. U. lactuca metabolites (polysaccharide and lectins) are also investigated for their ability to inhibit TNF-α synthesis and NO release in LPS-stimulated RAW 264.7 macrophages. The gastroprotective effect of the polysaccharide phase on inflammatory and ulcerative lesions of the gastric mucosa was estimated by the animal model of gastric alterations induced by intra- gastric
administration of the gastric mucosa of absolute ethanol. All of these studies was supported by minute
microscopic and macroscopic examinations. The results show that the polyphenol phase has proved an antioxidant potential in vitro. The polysaccharide and lectin phases showed a significant antiedema effect. Decreases in MPO activity and MDA levels in inflammatory rat foot tissues were significant in the case polysaccharide phase compared to lectins. Both phases increased the phagocytic activity of macrophages in vivo, although this increase was more pronounced in groups treated with the lectin phase. Both phases exerted a protective effect against LPS-induced inflammation in RAW264 cells and revealed a significant dependent dose down regulating the production of NO by macrophages, TNF-α release; these effects were more obvious in the case of the polysaccharide phase. The results
of this study showed that Algerian U. lactuca has an important immunomodulatory activity due specifically to the lectin fraction and an anti- inflammatory effect due specifically to the polysaccharide fraction. The pre-treatment of mice with polysaccharide extract preserves biomarkers of altered gastric mucosa and compensate the deficiency of antioxidant defense mechanisms, GSH, CAT, SOD and GST, and eliminates lipid peroxidation. The histological study similarly confirmed an important restoration of the architecture of the gastric mucosa. Thus, the Algerian U. lactuca is endowed with an antioxidant, anti-inflammatory and gastroprotective potential, these properties could
be due to the synergistic effects of its constituents including specifically lectins and polysaccharides
Diplôme : Doctorat En ligne : ../theses/biologie/MEZ7286.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10901 Potentiel antioxydant des algues des côtes de l’est Algérien. [texte imprimé] / Hichem Mezdour, Auteur ; Ahmed Menad, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2018 . - 119 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : U. lactuca polysaccharides lectins anti-inflammatoire immune-modulatoire antioxydant anti-inflammatory immuno-modulatory antioxidant ????? ??????? ???? ???????? ????????? ???? ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The number of studies of marine algae extracts from the Algerian species Ulva lactuca is so little. This study aims to examine the biological potential of polyphenoles, polysaccharide and lectins fractions of the species U. lactuca collected from eastern Algeria (Jijel). The polyphenoles fractions was evaluated by testing the antioxidant activity in vitro, the carrageenin-induced anti-inflammatory activity in the Albino wistar rat, and the immunomodulatory effect in vivo by assessing the index of phagocytosis. U. lactuca metabolites (polysaccharide and lectins) are also investigated for their ability to inhibit TNF-α synthesis and NO release in LPS-stimulated RAW 264.7 macrophages. The gastroprotective effect of the polysaccharide phase on inflammatory and ulcerative lesions of the gastric mucosa was estimated by the animal model of gastric alterations induced by intra- gastric
administration of the gastric mucosa of absolute ethanol. All of these studies was supported by minute
microscopic and macroscopic examinations. The results show that the polyphenol phase has proved an antioxidant potential in vitro. The polysaccharide and lectin phases showed a significant antiedema effect. Decreases in MPO activity and MDA levels in inflammatory rat foot tissues were significant in the case polysaccharide phase compared to lectins. Both phases increased the phagocytic activity of macrophages in vivo, although this increase was more pronounced in groups treated with the lectin phase. Both phases exerted a protective effect against LPS-induced inflammation in RAW264 cells and revealed a significant dependent dose down regulating the production of NO by macrophages, TNF-α release; these effects were more obvious in the case of the polysaccharide phase. The results
of this study showed that Algerian U. lactuca has an important immunomodulatory activity due specifically to the lectin fraction and an anti- inflammatory effect due specifically to the polysaccharide fraction. The pre-treatment of mice with polysaccharide extract preserves biomarkers of altered gastric mucosa and compensate the deficiency of antioxidant defense mechanisms, GSH, CAT, SOD and GST, and eliminates lipid peroxidation. The histological study similarly confirmed an important restoration of the architecture of the gastric mucosa. Thus, the Algerian U. lactuca is endowed with an antioxidant, anti-inflammatory and gastroprotective potential, these properties could
be due to the synergistic effects of its constituents including specifically lectins and polysaccharides
Diplôme : Doctorat En ligne : ../theses/biologie/MEZ7286.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10901 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MEZ/7286 MEZ/7286 Thèse Bibliothèque principale Thèses Disponible
