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Titre : Etude phytochimique et pharmacologique de deux plantes sahariennes : Helianthemum Lippii et Cymbopogon schoenanthus. Type de document : texte imprimé Auteurs : Nihed Djemam, Auteur ; Samir Benayache, Auteur Mention d'édition : 18-nov-20 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2020 Importance : 197 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Chimie: Chimie organique Chimie organique : Analyses physicochimiques, contrôle de la qualité et synthèse de substances bioactives Helianthemum lippii Cymbopogon schoenanthus séparations chromatographiques méthodes spectroscopiques huiles essentielles analyse HPLC-TOF/MS activité antioxydante activité anticancéreuse colon distal du rat effet relaxant chromatographic separations spectroscopic methods essential oils HPLC-TOF / MS analysis antioxidant activity anticancer activity rat distal colon relaxing effect التحليـل الكرمـاتوغرافي طرق التحليل الطيفـي الزيوت الأساسية النشاط المضادة للأكسدة النشـاط المضاد للسرطان القولون النازل تـحليل HPLC-TOF/MS تأثير الاسترخاء Index. décimale : 540 Chimie et sciences connexes Résumé :
This work is devoted to the phytochemical, biological and pharmacological study of two endemic Saharan species; Helianthemum lippii (Cistaceae) and Cymbopogon schoenanthus (Poaceae). The extraction of the two species followed by the chromatographic separations (CC, CCM and HPLC semi-preparative), led to isolate and identify using spectroscopic methods (1D and 2D NMR) 6 compounds among which two sterols, as well as four sesquiterpenes: Stigmasterol, β- Sitosterol, (1S,4aa)-4Methylene-6-(1-hydroxy-&-methyethyl)-8a-b-methyl-decahydroxydronaphtalene-Ab-ol 1S, 7S, 10S, eudesm-4(14)-en-1α-11α-diol(hydroxy-β-eudesmol), 4α, 11-dihydroxy-7-epimereudesmandiol (7-epi-cryptomeridiol), 4β, 7β, 11-enantioeudesmantriol (Pterodontriol), 4β,6β,10α-trihydroxy guaiane sesquiterpène (Teuclatriol). Chromatographic analysis of the essential oils of Cymbopogon schoenanthus showed that the hydrocarbons represented 26.7% while the sesquiterpene fraction reached 40%. The HPLC-TOF / MS analysis of the AcOEt and n-BuOH extracts of the two species suggested the presence of 32 phenolic acids and flavonoids which were present in different proportions The efficacy of inhibiting DNA damage and the evaluation of the anticancer activity carried out on the EtOAc and n-BuOH extracts of the two species demonstrated a particularity observed in Helianthemum lippii on the antioxidant effect and thus the antiproliferative effect. The pharmacological study was carried out on the effect of the extracts on the distal colon of Wistar rats; which have shown a marked relaxing effect on the isolated distal colon of the rat which could represent an underlying mechanism for the traditional use of these plants for gastrointestinal conditions. Further studies will help us understand the metabolites responsible for the effect and mechanism involved.
Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/chimie/DJE7702.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11536 Etude phytochimique et pharmacologique de deux plantes sahariennes : Helianthemum Lippii et Cymbopogon schoenanthus. [texte imprimé] / Nihed Djemam, Auteur ; Samir Benayache, Auteur . - 18-nov-20 . - جامعة الإخوة منتوري قسنطينة, 2020 . - 197 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Chimie: Chimie organique Chimie organique : Analyses physicochimiques, contrôle de la qualité et synthèse de substances bioactives Helianthemum lippii Cymbopogon schoenanthus séparations chromatographiques méthodes spectroscopiques huiles essentielles analyse HPLC-TOF/MS activité antioxydante activité anticancéreuse colon distal du rat effet relaxant chromatographic separations spectroscopic methods essential oils HPLC-TOF / MS analysis antioxidant activity anticancer activity rat distal colon relaxing effect التحليـل الكرمـاتوغرافي طرق التحليل الطيفـي الزيوت الأساسية النشاط المضادة للأكسدة النشـاط المضاد للسرطان القولون النازل تـحليل HPLC-TOF/MS تأثير الاسترخاء Index. décimale : 540 Chimie et sciences connexes Résumé :
This work is devoted to the phytochemical, biological and pharmacological study of two endemic Saharan species; Helianthemum lippii (Cistaceae) and Cymbopogon schoenanthus (Poaceae). The extraction of the two species followed by the chromatographic separations (CC, CCM and HPLC semi-preparative), led to isolate and identify using spectroscopic methods (1D and 2D NMR) 6 compounds among which two sterols, as well as four sesquiterpenes: Stigmasterol, β- Sitosterol, (1S,4aa)-4Methylene-6-(1-hydroxy-&-methyethyl)-8a-b-methyl-decahydroxydronaphtalene-Ab-ol 1S, 7S, 10S, eudesm-4(14)-en-1α-11α-diol(hydroxy-β-eudesmol), 4α, 11-dihydroxy-7-epimereudesmandiol (7-epi-cryptomeridiol), 4β, 7β, 11-enantioeudesmantriol (Pterodontriol), 4β,6β,10α-trihydroxy guaiane sesquiterpène (Teuclatriol). Chromatographic analysis of the essential oils of Cymbopogon schoenanthus showed that the hydrocarbons represented 26.7% while the sesquiterpene fraction reached 40%. The HPLC-TOF / MS analysis of the AcOEt and n-BuOH extracts of the two species suggested the presence of 32 phenolic acids and flavonoids which were present in different proportions The efficacy of inhibiting DNA damage and the evaluation of the anticancer activity carried out on the EtOAc and n-BuOH extracts of the two species demonstrated a particularity observed in Helianthemum lippii on the antioxidant effect and thus the antiproliferative effect. The pharmacological study was carried out on the effect of the extracts on the distal colon of Wistar rats; which have shown a marked relaxing effect on the isolated distal colon of the rat which could represent an underlying mechanism for the traditional use of these plants for gastrointestinal conditions. Further studies will help us understand the metabolites responsible for the effect and mechanism involved.
Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/chimie/DJE7702.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11536 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité DJE/7702 DJE/7702 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude chimique et biologique de plantes médicinales Algériennes : cas des Espèces Zizyphus lotus et Fagonia longispina. Type de document : texte imprimé Auteurs : Widad Ourzeddine, Auteur ; Samir Benayache, Directeur de thèse ; Fadila Benayache, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 232 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Fagonia longispina Zizyphus lotus huiles essentielles séparations chromatographiques méthodes spectroscopiques analyse GC-FID et GC-MS activité antioxydante activité antibactérienne essential oil different chromatographic
methods GC-FID and GC-MS analysis spectrophotometers methods antioxidant activity الفصل الكروماتوغرافي طرق التحليل الطيفي الزيوت الأساسية التحليل GC-FIDوGC-MS- النشاط المضاد للأكسدة النشاط المضاد للبكتيرياIndex. décimale : 540 Chimie et sciences connexes Résumé : The present work is devoted to the phytochemical and biological study of an endemic species of the septentrional Sahara Fagonia longispina Batt. (Zygophyllaceae) and the chemical composition and antioxidant activity study of fruit essential oil of Zizyphus lotus (L.) Desf. (Rhamnaceae).
In a first part, the phytochemical investigation of the species F. longispina using different chromatographic methods (CC, TLC and HPLC) led to isolation and identification by different spectroscopic methods (1D and 2D, MS and UV) 18 compounds. Among these isolated compounds, a new monoterpene ∆-2-carene-6-ol-6-O-(2',6'-diacetyl)-β-D-glucopyranoside, together with four other monoterpenes, seven flavonoids, four phenolic derivatives and two
sterol.
Moreover, the in vitro free radical-scavenging activity of the all extracts has been investigated by DPPH test, which have demonstrated that ethyl acetate extract are good sources of antioxidant compounds. However, all extracts of F. longispina showed no substantial result compared to the antioxidant activity of ascorbic acid. In addition, the total phenolic and the flavonoids contents were measured using spectrophotometers methods, the results showed that the acetate extract has a high content compared to the other extracts. As for antibacterial activity study, showed that a high antibacterial effect of the ethyl acetate extract.
In a second part, the chemical composition of fruit essential oil Z. lotus has been investigated by the GC-FID and GC-MS analysis, which led to identified 38 compounds. Fatty acids represented the major fraction (saturated and unsaturated compounds 67.8 and 11%, respectively), followed by hydrocarbons (10.8%) and sesquiterpenes (1.1%).These results were quite different from those obtained previously on the fixed oil of the fruit of this species from two samples from Algeria.
The antioxidant property of this oil was evaluated using β-carotene bleaching method. The result indicated the potent antioxidant activity of essential oils from Z. lotus.Diplôme : Doctorat En ligne : ../theses/chimie/OUR7236.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10831 Etude chimique et biologique de plantes médicinales Algériennes : cas des Espèces Zizyphus lotus et Fagonia longispina. [texte imprimé] / Widad Ourzeddine, Auteur ; Samir Benayache, Directeur de thèse ; Fadila Benayache, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 232 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Fagonia longispina Zizyphus lotus huiles essentielles séparations chromatographiques méthodes spectroscopiques analyse GC-FID et GC-MS activité antioxydante activité antibactérienne essential oil different chromatographic
methods GC-FID and GC-MS analysis spectrophotometers methods antioxidant activity الفصل الكروماتوغرافي طرق التحليل الطيفي الزيوت الأساسية التحليل GC-FIDوGC-MS- النشاط المضاد للأكسدة النشاط المضاد للبكتيرياIndex. décimale : 540 Chimie et sciences connexes Résumé : The present work is devoted to the phytochemical and biological study of an endemic species of the septentrional Sahara Fagonia longispina Batt. (Zygophyllaceae) and the chemical composition and antioxidant activity study of fruit essential oil of Zizyphus lotus (L.) Desf. (Rhamnaceae).
In a first part, the phytochemical investigation of the species F. longispina using different chromatographic methods (CC, TLC and HPLC) led to isolation and identification by different spectroscopic methods (1D and 2D, MS and UV) 18 compounds. Among these isolated compounds, a new monoterpene ∆-2-carene-6-ol-6-O-(2',6'-diacetyl)-β-D-glucopyranoside, together with four other monoterpenes, seven flavonoids, four phenolic derivatives and two
sterol.
Moreover, the in vitro free radical-scavenging activity of the all extracts has been investigated by DPPH test, which have demonstrated that ethyl acetate extract are good sources of antioxidant compounds. However, all extracts of F. longispina showed no substantial result compared to the antioxidant activity of ascorbic acid. In addition, the total phenolic and the flavonoids contents were measured using spectrophotometers methods, the results showed that the acetate extract has a high content compared to the other extracts. As for antibacterial activity study, showed that a high antibacterial effect of the ethyl acetate extract.
In a second part, the chemical composition of fruit essential oil Z. lotus has been investigated by the GC-FID and GC-MS analysis, which led to identified 38 compounds. Fatty acids represented the major fraction (saturated and unsaturated compounds 67.8 and 11%, respectively), followed by hydrocarbons (10.8%) and sesquiterpenes (1.1%).These results were quite different from those obtained previously on the fixed oil of the fruit of this species from two samples from Algeria.
The antioxidant property of this oil was evaluated using β-carotene bleaching method. The result indicated the potent antioxidant activity of essential oils from Z. lotus.Diplôme : Doctorat En ligne : ../theses/chimie/OUR7236.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10831 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité OUR/7236 OUR/7236 Thèse Bibliothèque principale Thèses Disponible
