| Titre : |
Extraction, Isolation and Characterization of Lectins from Moringa oleifera seeds |
| Type de document : |
texte imprimé |
| Auteurs : |
Chaima Benseghir, Auteur ; Phiona Namoulodi, Auteur ; Y Necib, Directeur de thèse |
| Editeur : |
CONSTANTINE [ALGERIE] : Université Frères Mentouri Constantine |
| Année de publication : |
2018 |
| Importance : |
77 f. |
| Format : |
30 cm. |
| Note générale : |
une copie electronique pdf disponible au buc. |
| Langues : |
Français (fre) |
| Catégories : |
Biologie:Biochimie et Biologie Cellulaire et Moléculaire
|
| Tags : |
: Lectins, Extraction, Agglutination, Characterization, Moringa oleifera, Inhibition test |
| Index. décimale : |
730 Biochimie et biologie cellulaire |
| Résumé : |
The purpose of the research was to study the isolation, purification and
characterization of lectins from mature Moringa oleifera seeds. The lectins were extracted
from the seeds with phosphate-buffered saline (PBS) at pH 7.4, and partial purification
was accomplished by ammonium sulfate precipitation and gel filtration using Sephadex G-
50.
Agglutination using glutaraldehyde-treated rabbit erythrocytes and human blood types A,
B, O was carried to determine the presence and specificity of the lectin. Subsequently, the
Moringa oleifera seed lectins were classified as a complete non-specific lectin since it
agglutinated all human blood types and glutaldehyde-treated rabbit erythrocytes.
The results of carbohydrate specificity showed that the lectins had complex sugar
specificity to fetuin. The lectins were found to be thermally stable, and stable within the
pH range of 3-6. It was also considered as a non metallolectin since its activity was not
significantly affected by EDTA |
| Diplome : |
Master 2 |
| Permalink : |
https://bu.umc.edu.dz/master/index.php?lvl=notice_display&id=10586 |
Extraction, Isolation and Characterization of Lectins from Moringa oleifera seeds [texte imprimé] / Chaima Benseghir, Auteur ; Phiona Namoulodi, Auteur ; Y Necib, Directeur de thèse . - CONSTANTINE [ALGERIE] : Université Frères Mentouri Constantine, 2018 . - 77 f. ; 30 cm. une copie electronique pdf disponible au buc. Langues : Français ( fre)
| Catégories : |
Biologie:Biochimie et Biologie Cellulaire et Moléculaire
|
| Tags : |
: Lectins, Extraction, Agglutination, Characterization, Moringa oleifera, Inhibition test |
| Index. décimale : |
730 Biochimie et biologie cellulaire |
| Résumé : |
The purpose of the research was to study the isolation, purification and
characterization of lectins from mature Moringa oleifera seeds. The lectins were extracted
from the seeds with phosphate-buffered saline (PBS) at pH 7.4, and partial purification
was accomplished by ammonium sulfate precipitation and gel filtration using Sephadex G-
50.
Agglutination using glutaraldehyde-treated rabbit erythrocytes and human blood types A,
B, O was carried to determine the presence and specificity of the lectin. Subsequently, the
Moringa oleifera seed lectins were classified as a complete non-specific lectin since it
agglutinated all human blood types and glutaldehyde-treated rabbit erythrocytes.
The results of carbohydrate specificity showed that the lectins had complex sugar
specificity to fetuin. The lectins were found to be thermally stable, and stable within the
pH range of 3-6. It was also considered as a non metallolectin since its activity was not
significantly affected by EDTA |
| Diplome : |
Master 2 |
| Permalink : |
https://bu.umc.edu.dz/master/index.php?lvl=notice_display&id=10586 |
|
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