| Titre : |
Accumulation de triglycerides par la levure oleagineuse Yarrowia Lipolytica : maitrise et suivi des processus biologiques |
| Type de document : |
texte imprimé |
| Auteurs : |
Doria Naila Bouchedja (Née Benzeghda), Auteur ; Abdelghani Boudjellal, Directeur de thèse |
| Editeur : |
جامعة الإخوة منتوري قسنطينة |
| Année de publication : |
2017 |
| Importance : |
136 f. |
| Format : |
30 cm. |
| Note générale : |
2 copies imprimées disponibles
|
| Langues : |
Français (fre) |
| Catégories : |
Français - Anglais
Nutrition, Alimentation et Technologies Agro-Alimentaires
|
| Tags : |
Yarrowia lipolytica Accumulation lipidique Assimilation lipidique Croissance microbienne Dimorphisme cellulaire Cytométrie en flux en ligne Métabolisme lipidique levure oléagineuse Lipid accumulation Lipid assimilation Microbial growth Cell dimorphism On-line flow cytometry Lipid metabolism Oleaginous yeasts تخزين الدهون استيعاب الدهون والنمو الميكروبي إزدواج
المرفلوجيا الخلوية السيتومترية استقلاب الدهون الخميرة الزيتية |
| Index. décimale : |
636 Sciences Vétérinaires |
| Résumé : |
The objective of the present thesis deals mainly with the study of the incorporation
and the accumulation of lipids by the fatty yeast Yarrowia lipolytica seen in different aspects.
The work was emphasized through the following aspects: growth and lipid accumulation of
cultured yeast in four different media types, effect of pH on the foaming phenomenon of the
culture medium in the bioreactor, effect of culture parameters on the different phenomena
occurring during a culture of the studied microorganism, through the development of an original
technique consisting of an in-line coupling between bioreactors and a flow cytometer. The latter
allowed the on-line and continuous monitoring of the effect of crop parameters and finally the
investigation of cell growth and lipid incorporation in three genetically modified strains of Y.
lipolytica. The study revealed that yeast had the ability to grow and accumulate lipids from very
different culture media, including those consisting of industrial by-products. However, the rates
of growth, incorporation and lipid accumulation differed according to the strain involved. The
technique of in-line cytometry has shed light on the effect of aeration and pH on cellular growth
phenomena of lipid accumulation and dimorphism in Y.lipolytica. High oxygenation had an
accelerating effect on growth and metabolization of accumulated lipids, while a critical pH level
had a downright inhibitory effect. The transition from mycelial to non-mycelial round was also
influenced by oxygen and pH. The modulation of the latter has proven to be effective in
controlling the foaming phenomenon in bioreactors. Therefore, by modifying the pH values, it
was possible to neutralize the foam without affecting the microorganism. |
| Diplôme : |
Doctorat en sciences |
| En ligne : |
../theses/agronomie/BOU7149.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10454 |
Accumulation de triglycerides par la levure oleagineuse Yarrowia Lipolytica : maitrise et suivi des processus biologiques [texte imprimé] / Doria Naila Bouchedja (Née Benzeghda), Auteur ; Abdelghani Boudjellal, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 136 f. ; 30 cm. 2 copies imprimées disponibles
Langues : Français ( fre)
| Catégories : |
Français - Anglais
Nutrition, Alimentation et Technologies Agro-Alimentaires
|
| Tags : |
Yarrowia lipolytica Accumulation lipidique Assimilation lipidique Croissance microbienne Dimorphisme cellulaire Cytométrie en flux en ligne Métabolisme lipidique levure oléagineuse Lipid accumulation Lipid assimilation Microbial growth Cell dimorphism On-line flow cytometry Lipid metabolism Oleaginous yeasts تخزين الدهون استيعاب الدهون والنمو الميكروبي إزدواج
المرفلوجيا الخلوية السيتومترية استقلاب الدهون الخميرة الزيتية |
| Index. décimale : |
636 Sciences Vétérinaires |
| Résumé : |
The objective of the present thesis deals mainly with the study of the incorporation
and the accumulation of lipids by the fatty yeast Yarrowia lipolytica seen in different aspects.
The work was emphasized through the following aspects: growth and lipid accumulation of
cultured yeast in four different media types, effect of pH on the foaming phenomenon of the
culture medium in the bioreactor, effect of culture parameters on the different phenomena
occurring during a culture of the studied microorganism, through the development of an original
technique consisting of an in-line coupling between bioreactors and a flow cytometer. The latter
allowed the on-line and continuous monitoring of the effect of crop parameters and finally the
investigation of cell growth and lipid incorporation in three genetically modified strains of Y.
lipolytica. The study revealed that yeast had the ability to grow and accumulate lipids from very
different culture media, including those consisting of industrial by-products. However, the rates
of growth, incorporation and lipid accumulation differed according to the strain involved. The
technique of in-line cytometry has shed light on the effect of aeration and pH on cellular growth
phenomena of lipid accumulation and dimorphism in Y.lipolytica. High oxygenation had an
accelerating effect on growth and metabolization of accumulated lipids, while a critical pH level
had a downright inhibitory effect. The transition from mycelial to non-mycelial round was also
influenced by oxygen and pH. The modulation of the latter has proven to be effective in
controlling the foaming phenomenon in bioreactors. Therefore, by modifying the pH values, it
was possible to neutralize the foam without affecting the microorganism. |
| Diplôme : |
Doctorat en sciences |
| En ligne : |
../theses/agronomie/BOU7149.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10454 |
|
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