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Auteur Meriem Meziani |
Documents disponibles écrits par cet auteur (3)
Affiner la recherche Interroger des sources externesAnalyse moléculaire et étude de la résistance aux antibiotiques des souches d’Escherichia coli productrices de β-lactamases à spectre étendu (BLSE) isolées au CHU de Constantine. / Meriem Meziani
Titre : Analyse moléculaire et étude de la résistance aux antibiotiques des souches d’Escherichia coli productrices de β-lactamases à spectre étendu (BLSE) isolées au CHU de Constantine. Type de document : texte imprimé Auteurs : Meriem Meziani, Auteur ; Kaddour Benlabed, Directeur de thèse Mention d'édition : 08/04/2021 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2021 Importance : 106 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Microbiologie: Biochimie microbienne E. coli résistance aux antibiotiques BLSE 16S ARNr méthylases (armA) CMY-16 OXA-48 CHU de Constantine antibiotic resistance 16S rRNA methylases (armA) CHU of Constantine مقاومة المضادات الحيوية Index. décimale : 570 Sciences de la vie. Biologie Résumé :
The species Escherichia coli is among the bacteria responsible for nosocomial infections and epidemics. The resistance of E. coli to antibiotics is undergoing a worrying worldwide development due to the production of extended spectrum β-lactamases (ESBLs). These microorganisms, responsible for nosocomial infections, are found to be resistant to several families of antibiotics such as β-lactams and aminoglycosides. The increase and spread of antibiotic resistance in this species represents a major global public health problem. The aim of this study was to investigate the antibiotic resistance of strains of E. coli isolated at the University Hospital of Constantine between 2013-2015, and the characterization of AmpC genes as well as the study of the coexistence of 16S rRNA methylase genes and carbapenemases in ESBL-producing strains of Escherichia coli.
235 strains of E. coli were collected and identification was performed using standard biochemical galleries and confirmed by mass spectrometry (MALDI-TOF-MS). Then, the antibiotic sensitivity study (method of diffusion of discs on agar) was carried out according to the recommendations of CLSI. Phenotypic research of the production of extended spectrum β- lactamases (ESBL) and carbapenemases was also performed. In addition, the antibiotic resistance genes have been characterized by Molecular Biology techniques (PCR and sequencing). Next, the determination of the ST131 clone and the phylogenetic groups of the strains of ESBL E. coli was carried out using respectively the specific PCR targeting the papB gene according to the method of Clermont et al., 2009 for the detection of the ST131 clone and the PCR of phylogroups according to the revised method of Clermont et al., 2013. In this study, we report for the first time the description of E. coli strains co-expressing both the ArmA, OXA-48, CMY-16 and TEM-1 genes. These strains isolated at the University Hospital of Constantine were resistant to β-lactams and aminoglycosides by production of ESBLs, cephalosporinases, carbapenemases and 16S rRNA methylases. Which shows the increased frequency of multidrug-resistant strains of E. coli, and which requires establishing control measures in Algerian hospitals to prevent their spread.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MEZ7772.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11606 Analyse moléculaire et étude de la résistance aux antibiotiques des souches d’Escherichia coli productrices de β-lactamases à spectre étendu (BLSE) isolées au CHU de Constantine. [texte imprimé] / Meriem Meziani, Auteur ; Kaddour Benlabed, Directeur de thèse . - 08/04/2021 . - جامعة الإخوة منتوري قسنطينة, 2021 . - 106 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Microbiologie: Biochimie microbienne E. coli résistance aux antibiotiques BLSE 16S ARNr méthylases (armA) CMY-16 OXA-48 CHU de Constantine antibiotic resistance 16S rRNA methylases (armA) CHU of Constantine مقاومة المضادات الحيوية Index. décimale : 570 Sciences de la vie. Biologie Résumé :
The species Escherichia coli is among the bacteria responsible for nosocomial infections and epidemics. The resistance of E. coli to antibiotics is undergoing a worrying worldwide development due to the production of extended spectrum β-lactamases (ESBLs). These microorganisms, responsible for nosocomial infections, are found to be resistant to several families of antibiotics such as β-lactams and aminoglycosides. The increase and spread of antibiotic resistance in this species represents a major global public health problem. The aim of this study was to investigate the antibiotic resistance of strains of E. coli isolated at the University Hospital of Constantine between 2013-2015, and the characterization of AmpC genes as well as the study of the coexistence of 16S rRNA methylase genes and carbapenemases in ESBL-producing strains of Escherichia coli.
235 strains of E. coli were collected and identification was performed using standard biochemical galleries and confirmed by mass spectrometry (MALDI-TOF-MS). Then, the antibiotic sensitivity study (method of diffusion of discs on agar) was carried out according to the recommendations of CLSI. Phenotypic research of the production of extended spectrum β- lactamases (ESBL) and carbapenemases was also performed. In addition, the antibiotic resistance genes have been characterized by Molecular Biology techniques (PCR and sequencing). Next, the determination of the ST131 clone and the phylogenetic groups of the strains of ESBL E. coli was carried out using respectively the specific PCR targeting the papB gene according to the method of Clermont et al., 2009 for the detection of the ST131 clone and the PCR of phylogroups according to the revised method of Clermont et al., 2013. In this study, we report for the first time the description of E. coli strains co-expressing both the ArmA, OXA-48, CMY-16 and TEM-1 genes. These strains isolated at the University Hospital of Constantine were resistant to β-lactams and aminoglycosides by production of ESBLs, cephalosporinases, carbapenemases and 16S rRNA methylases. Which shows the increased frequency of multidrug-resistant strains of E. coli, and which requires establishing control measures in Algerian hospitals to prevent their spread.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MEZ7772.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11606 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MEZ/7772 MEZ/7772 Thèse Bibliothèque principale Thèses Disponible
Titre : Contribution du diagnostic biochimique bactérien dans l’établissement des parentés phylogénétiques : Cas des Entérobactéries et Pseudomonas. Type de document : texte imprimé Auteurs : Meriem Meziani, Auteur ; M. A Hamidechi, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2012 Importance : 79 f. Format : 31 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biochimie:Technologie des explorations biochimiques Entérobactéries phénotype biochimique phylogénie Enterobacteria Biochemical phenotype Phylogeny الامعائیات النمط الظاھري البیوكیمیائي نسالة Index. décimale : 570 Sciences de la vie. Biologie Résumé : Ten bacterial strains were isolated from clinical specimens provided by the bacteriology laboratory (DAKSI). They were identified by standard microbiological methods (biochemical gallery). Among the ten strains studied, eight strains belonging to the Enterobacteriaceae and two strains are among the Gram-negative bacilli non-fermentative (Pseudomonas). These strains were identified presumptively by their biochemical characteristics, susceptibility or resistance to antibiotics, and total nitrogen measured by the Kjeldahl method.
A phylogenetic profile was produced by phenetic distance methods, using NJ algorithm and sequence analysis of 16S DNA. The analysis of topologies obtained was used to determine the place of the biochemical phenotype in establishing phylogenetic relationships.Note de contenu : Annexes. Diplôme : Magistère En ligne : ../theses/biologie/MEZ6113.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=6028 Contribution du diagnostic biochimique bactérien dans l’établissement des parentés phylogénétiques : Cas des Entérobactéries et Pseudomonas. [texte imprimé] / Meriem Meziani, Auteur ; M. A Hamidechi, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2012 . - 79 f. ; 31 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biochimie:Technologie des explorations biochimiques Entérobactéries phénotype biochimique phylogénie Enterobacteria Biochemical phenotype Phylogeny الامعائیات النمط الظاھري البیوكیمیائي نسالة Index. décimale : 570 Sciences de la vie. Biologie Résumé : Ten bacterial strains were isolated from clinical specimens provided by the bacteriology laboratory (DAKSI). They were identified by standard microbiological methods (biochemical gallery). Among the ten strains studied, eight strains belonging to the Enterobacteriaceae and two strains are among the Gram-negative bacilli non-fermentative (Pseudomonas). These strains were identified presumptively by their biochemical characteristics, susceptibility or resistance to antibiotics, and total nitrogen measured by the Kjeldahl method.
A phylogenetic profile was produced by phenetic distance methods, using NJ algorithm and sequence analysis of 16S DNA. The analysis of topologies obtained was used to determine the place of the biochemical phenotype in establishing phylogenetic relationships.Note de contenu : Annexes. Diplôme : Magistère En ligne : ../theses/biologie/MEZ6113.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=6028 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MEZ/6113 MEZ/6113 Thèse Bibliothèque principale Thèses Disponible
Titre : RACISM in Joseph Conrad’s heart of darkness Type de document : texte imprimé Auteurs : Meriem Meziani, Auteur ; Mohammed Yachir, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2010 Importance : 50 f. Format : 31 cm Note générale : 2 copies imprimées disponibles Langues : Anglais (eng) Catégories : Français - Anglais
Langue AnglaiseTags : Langue Anglaise Index. décimale : 420 Anglais et vieil anglais (anglo-saxon) : indice de base 42 Résumé : This paper deals with the concept of racism, which is considered as a dark
chapter in the history of the world .Throughout history, racist ideology widespread
throughout the world especially between blacks and whites. In addition, many
European countries started to expand their empire and to get more territories in other
countries .
Joseph Conrad’s Heart of Darkness which is his experience in the Congo River during
the 19th century dealt with the concept of racism, which was clear in this novel because
of the conflicts that were between black and whites and it explained the real aims o
colonialism and expansionism in Africa, which were for wealth and power.Diplôme : Master 2 En ligne : ../theses/anglais/MEZ1234.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=5751 RACISM in Joseph Conrad’s heart of darkness [texte imprimé] / Meriem Meziani, Auteur ; Mohammed Yachir, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2010 . - 50 f. ; 31 cm.
2 copies imprimées disponibles
Langues : Anglais (eng)
Catégories : Français - Anglais
Langue AnglaiseTags : Langue Anglaise Index. décimale : 420 Anglais et vieil anglais (anglo-saxon) : indice de base 42 Résumé : This paper deals with the concept of racism, which is considered as a dark
chapter in the history of the world .Throughout history, racist ideology widespread
throughout the world especially between blacks and whites. In addition, many
European countries started to expand their empire and to get more territories in other
countries .
Joseph Conrad’s Heart of Darkness which is his experience in the Congo River during
the 19th century dealt with the concept of racism, which was clear in this novel because
of the conflicts that were between black and whites and it explained the real aims o
colonialism and expansionism in Africa, which were for wealth and power.Diplôme : Master 2 En ligne : ../theses/anglais/MEZ1234.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=5751 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MEZ/1234 MEZ/1234 Thèse Bibliothèque principale Thèses Disponible
