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Partager le résultat de cette recherche Interroger des sources externesCaractérisation des souches de Staphylococcus aureus résistantes à la méticilline (SARM) et évaluation de leur sensibilité à la propolis. / Mounira Adoui
Titre : Caractérisation des souches de Staphylococcus aureus résistantes à la méticilline (SARM) et évaluation de leur sensibilité à la propolis. Type de document : texte imprimé Auteurs : Mounira Adoui, Auteur ; Mesbah Lahouel, Directeur de thèse Mention d'édition : 08/04/2019 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 123 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Science biologique :Microbiologie Résistance aux antibiotiques SARM mecA EEP synergie antibiotique propolis Antibiotic resistance MRSA antibiotic-propolis synergy مقاومة المضادات الحيوية SARMالتاثير التآزري للمضادات
الحيويةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Methicillin-resistant Staphylococcus aureus (MRSA) has come to be one of the primary causes of nosocomial and community infections worldwide. The main objective of this thesis was to evaluate the antibacterial activity of propolis ethanol extract EEP with respect to multidrug-resistant bacteria (MRSA). In order to achieve so, clinically isolated MRSA strains had been selected and then studied their resistance profile to different antibiotics families. Methicillin resistance detection has been completed by looking for mecA gene via PCR. The synergy among EEP and 12 anti-staphylococcal antibiotics has also been studied. Antibiotic sensibility study allowed us to detect 34 strains of S. aureus resistant to methicillin MRSA (21.11%) from diverse samples: pus, blood cultures, urine, vaginal samples and
equipment (catheters). The resistance profile analysis of antibiotic MRSA approved the multi-resistant nature of these bacteria to different antibiotics families: aminoglycosides, macrolides and fluoroquinolones. DNA extracts Analysis of 24 MRSA strains by amplification indicated the presence of the mecA gene in 21 strains, showing that this gene was responsible for methicillin resistance in these strains. Antibacterial activity assessment of propolis revealed that EEP exhibited worthy antibacterial activity with respect to all MSSA and MRSA strains. The average MIC and CMB values against the MSSA strains were respectively 0.55 ± 0.45 mg/ml and 5.5 ± 4.5 mg/ml. In the case of MRSA strains, the MIC and CMB were correspondingly: 0.67 ± 0.41 mg/ ml and 6.72 ± 4.16 mg/ml. The addition of EEP at a concentration of 0.25 mg/ml in the medium improved the MRSA strains sensibility to all antibiotics. This analysis showed a synergy between EPP and all tested antibiotics. On the other hand, no case of antagonism between PEF and tested antibiotics was recorded. This reported synergy was relevant, and propolis can be an alternative for these pathogens treatment.Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ADO7565.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11403 Caractérisation des souches de Staphylococcus aureus résistantes à la méticilline (SARM) et évaluation de leur sensibilité à la propolis. [texte imprimé] / Mounira Adoui, Auteur ; Mesbah Lahouel, Directeur de thèse . - 08/04/2019 . - جامعة الإخوة منتوري قسنطينة, 2019 . - 123 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Science biologique :Microbiologie Résistance aux antibiotiques SARM mecA EEP synergie antibiotique propolis Antibiotic resistance MRSA antibiotic-propolis synergy مقاومة المضادات الحيوية SARMالتاثير التآزري للمضادات
الحيويةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Methicillin-resistant Staphylococcus aureus (MRSA) has come to be one of the primary causes of nosocomial and community infections worldwide. The main objective of this thesis was to evaluate the antibacterial activity of propolis ethanol extract EEP with respect to multidrug-resistant bacteria (MRSA). In order to achieve so, clinically isolated MRSA strains had been selected and then studied their resistance profile to different antibiotics families. Methicillin resistance detection has been completed by looking for mecA gene via PCR. The synergy among EEP and 12 anti-staphylococcal antibiotics has also been studied. Antibiotic sensibility study allowed us to detect 34 strains of S. aureus resistant to methicillin MRSA (21.11%) from diverse samples: pus, blood cultures, urine, vaginal samples and
equipment (catheters). The resistance profile analysis of antibiotic MRSA approved the multi-resistant nature of these bacteria to different antibiotics families: aminoglycosides, macrolides and fluoroquinolones. DNA extracts Analysis of 24 MRSA strains by amplification indicated the presence of the mecA gene in 21 strains, showing that this gene was responsible for methicillin resistance in these strains. Antibacterial activity assessment of propolis revealed that EEP exhibited worthy antibacterial activity with respect to all MSSA and MRSA strains. The average MIC and CMB values against the MSSA strains were respectively 0.55 ± 0.45 mg/ml and 5.5 ± 4.5 mg/ml. In the case of MRSA strains, the MIC and CMB were correspondingly: 0.67 ± 0.41 mg/ ml and 6.72 ± 4.16 mg/ml. The addition of EEP at a concentration of 0.25 mg/ml in the medium improved the MRSA strains sensibility to all antibiotics. This analysis showed a synergy between EPP and all tested antibiotics. On the other hand, no case of antagonism between PEF and tested antibiotics was recorded. This reported synergy was relevant, and propolis can be an alternative for these pathogens treatment.Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ADO7565.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11403 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ADO/7565 ADO/7565 Thèse Bibliothèque principale Thèses Disponible
Titre : Analyse moléculaire et étude de la résistance aux antibiotiques des souches d’Escherichia coli productrices de β-lactamases à spectre étendu (BLSE) isolées au CHU de Constantine. Type de document : texte imprimé Auteurs : Meriem Meziani, Auteur ; Kaddour Benlabed, Directeur de thèse Mention d'édition : 08/04/2021 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2021 Importance : 106 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Microbiologie: Biochimie microbienne E. coli résistance aux antibiotiques BLSE 16S ARNr méthylases (armA) CMY-16 OXA-48 CHU de Constantine antibiotic resistance 16S rRNA methylases (armA) CHU of Constantine مقاومة المضادات الحيوية Index. décimale : 570 Sciences de la vie. Biologie Résumé :
The species Escherichia coli is among the bacteria responsible for nosocomial infections and epidemics. The resistance of E. coli to antibiotics is undergoing a worrying worldwide development due to the production of extended spectrum β-lactamases (ESBLs). These microorganisms, responsible for nosocomial infections, are found to be resistant to several families of antibiotics such as β-lactams and aminoglycosides. The increase and spread of antibiotic resistance in this species represents a major global public health problem. The aim of this study was to investigate the antibiotic resistance of strains of E. coli isolated at the University Hospital of Constantine between 2013-2015, and the characterization of AmpC genes as well as the study of the coexistence of 16S rRNA methylase genes and carbapenemases in ESBL-producing strains of Escherichia coli.
235 strains of E. coli were collected and identification was performed using standard biochemical galleries and confirmed by mass spectrometry (MALDI-TOF-MS). Then, the antibiotic sensitivity study (method of diffusion of discs on agar) was carried out according to the recommendations of CLSI. Phenotypic research of the production of extended spectrum β- lactamases (ESBL) and carbapenemases was also performed. In addition, the antibiotic resistance genes have been characterized by Molecular Biology techniques (PCR and sequencing). Next, the determination of the ST131 clone and the phylogenetic groups of the strains of ESBL E. coli was carried out using respectively the specific PCR targeting the papB gene according to the method of Clermont et al., 2009 for the detection of the ST131 clone and the PCR of phylogroups according to the revised method of Clermont et al., 2013. In this study, we report for the first time the description of E. coli strains co-expressing both the ArmA, OXA-48, CMY-16 and TEM-1 genes. These strains isolated at the University Hospital of Constantine were resistant to β-lactams and aminoglycosides by production of ESBLs, cephalosporinases, carbapenemases and 16S rRNA methylases. Which shows the increased frequency of multidrug-resistant strains of E. coli, and which requires establishing control measures in Algerian hospitals to prevent their spread.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MEZ7772.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11606 Analyse moléculaire et étude de la résistance aux antibiotiques des souches d’Escherichia coli productrices de β-lactamases à spectre étendu (BLSE) isolées au CHU de Constantine. [texte imprimé] / Meriem Meziani, Auteur ; Kaddour Benlabed, Directeur de thèse . - 08/04/2021 . - جامعة الإخوة منتوري قسنطينة, 2021 . - 106 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Microbiologie: Biochimie microbienne E. coli résistance aux antibiotiques BLSE 16S ARNr méthylases (armA) CMY-16 OXA-48 CHU de Constantine antibiotic resistance 16S rRNA methylases (armA) CHU of Constantine مقاومة المضادات الحيوية Index. décimale : 570 Sciences de la vie. Biologie Résumé :
The species Escherichia coli is among the bacteria responsible for nosocomial infections and epidemics. The resistance of E. coli to antibiotics is undergoing a worrying worldwide development due to the production of extended spectrum β-lactamases (ESBLs). These microorganisms, responsible for nosocomial infections, are found to be resistant to several families of antibiotics such as β-lactams and aminoglycosides. The increase and spread of antibiotic resistance in this species represents a major global public health problem. The aim of this study was to investigate the antibiotic resistance of strains of E. coli isolated at the University Hospital of Constantine between 2013-2015, and the characterization of AmpC genes as well as the study of the coexistence of 16S rRNA methylase genes and carbapenemases in ESBL-producing strains of Escherichia coli.
235 strains of E. coli were collected and identification was performed using standard biochemical galleries and confirmed by mass spectrometry (MALDI-TOF-MS). Then, the antibiotic sensitivity study (method of diffusion of discs on agar) was carried out according to the recommendations of CLSI. Phenotypic research of the production of extended spectrum β- lactamases (ESBL) and carbapenemases was also performed. In addition, the antibiotic resistance genes have been characterized by Molecular Biology techniques (PCR and sequencing). Next, the determination of the ST131 clone and the phylogenetic groups of the strains of ESBL E. coli was carried out using respectively the specific PCR targeting the papB gene according to the method of Clermont et al., 2009 for the detection of the ST131 clone and the PCR of phylogroups according to the revised method of Clermont et al., 2013. In this study, we report for the first time the description of E. coli strains co-expressing both the ArmA, OXA-48, CMY-16 and TEM-1 genes. These strains isolated at the University Hospital of Constantine were resistant to β-lactams and aminoglycosides by production of ESBLs, cephalosporinases, carbapenemases and 16S rRNA methylases. Which shows the increased frequency of multidrug-resistant strains of E. coli, and which requires establishing control measures in Algerian hospitals to prevent their spread.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MEZ7772.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11606 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MEZ/7772 MEZ/7772 Thèse Bibliothèque principale Thèses Disponible
Titre : Contribution à l'étude des infections intramammaires de la vache laitiere dans l'Est algeriens Type de document : texte imprimé Auteurs : Omar Bouaziz, Auteur ; F. Smati, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2005 Importance : 235 f. Format : 30 cm. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la bibliothèque centrale
01 CDLangues : Français (fre) Catégories : Français - Anglais
Sciences VétérinairesTags : Bactériologie Vache laitière Antibiorésistance Mammite clinique Mammite subclinique CMT Résidus d'antibiotiques Traitement au tarissement dairy cattle clinical mastitis subclinical mastitis bacteriolgy Antibiotic residues antibiotic resistance dry cow therapy Index. décimale : 636 Sciences Vétérinaires Résumé : The control of the infections intramammaires represents a paramount stake for the dairy stockbreeders. To fight against this pathology passes by a knowledge of the bacteria in causes and their epidemiology.
The present study related on the one hand, on the evaluation of the prévalence of the germs responsible for the clinical mastitis and the study of some aspects of their epidemiology in 35 dairy herds and on the other hand, to evaluate the prévalence and the etiology of the subclinic mastitis in the cows at the end of the lactation in four dairy herds. The performance of the CMT to identify subclincal mastitis at the end of the lactation was evaluated. The in vitro antimicrobial susceptibility of bacteria isolated from cow milk samples was tested. Treatment of dairy cows at drying off has been practiced.
Results obtained showed :
A strong prévalence (32,6%) of the clinical cows with clinical mastitis. The bacteriological analysis of 252 milk samples milk showed : 28,4% of Staphylococcus aureus, 21,6% of Eschérichia coli, 13,7% of Streptococcus agalactiae. The coagulase negative staphylococci with a frequency of 10,8% seem increasingly accused in the clinical mastitis.
The prévalence of the cows with subclnical mastitis at the end of the lactation is 73,6% and the quarters 40,3%. The frequency of the germs responsible for subclnical mastitis was estimated on 121 cows at the end of the lactation. Out of he 464 milk samples, 59,7% were bacteriological negative. The germs observed most often are :
Staphylococcus aureus (30,9%), coagulase negative staphylococci (25,9%), Streptococcus agalactiae (23,2%) and Eschérichia coli (15,9%).
Staphylococcus aureus is the predominant bacterial pathogen in subclinical and clinical mastitis. Environmental pathogens are not to neglect in the cause of the clinical mastits.
Moreover, this study revealed the presence of antibiotic residues in 17,1% of the 35 milk samples analyzed.
The in vitro antimicrobial susceptibility of bacteria isolated from cow milk samples showed high resistance to penicillin, ampicillin, oxacillin, tetracyclin, streptomycin and erythromycin.
During the dry period, the treatment eliminates 91% of pathogenic organism. The rate of new infections prevented is 88%.Diplôme : Doctorat En ligne : ../theses/veterinaire/BOU4260.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1887 Contribution à l'étude des infections intramammaires de la vache laitiere dans l'Est algeriens [texte imprimé] / Omar Bouaziz, Auteur ; F. Smati, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2005 . - 235 f. ; 30 cm.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la bibliothèque centrale
01 CD
Langues : Français (fre)
Catégories : Français - Anglais
Sciences VétérinairesTags : Bactériologie Vache laitière Antibiorésistance Mammite clinique Mammite subclinique CMT Résidus d'antibiotiques Traitement au tarissement dairy cattle clinical mastitis subclinical mastitis bacteriolgy Antibiotic residues antibiotic resistance dry cow therapy Index. décimale : 636 Sciences Vétérinaires Résumé : The control of the infections intramammaires represents a paramount stake for the dairy stockbreeders. To fight against this pathology passes by a knowledge of the bacteria in causes and their epidemiology.
The present study related on the one hand, on the evaluation of the prévalence of the germs responsible for the clinical mastitis and the study of some aspects of their epidemiology in 35 dairy herds and on the other hand, to evaluate the prévalence and the etiology of the subclinic mastitis in the cows at the end of the lactation in four dairy herds. The performance of the CMT to identify subclincal mastitis at the end of the lactation was evaluated. The in vitro antimicrobial susceptibility of bacteria isolated from cow milk samples was tested. Treatment of dairy cows at drying off has been practiced.
Results obtained showed :
A strong prévalence (32,6%) of the clinical cows with clinical mastitis. The bacteriological analysis of 252 milk samples milk showed : 28,4% of Staphylococcus aureus, 21,6% of Eschérichia coli, 13,7% of Streptococcus agalactiae. The coagulase negative staphylococci with a frequency of 10,8% seem increasingly accused in the clinical mastitis.
The prévalence of the cows with subclnical mastitis at the end of the lactation is 73,6% and the quarters 40,3%. The frequency of the germs responsible for subclnical mastitis was estimated on 121 cows at the end of the lactation. Out of he 464 milk samples, 59,7% were bacteriological negative. The germs observed most often are :
Staphylococcus aureus (30,9%), coagulase negative staphylococci (25,9%), Streptococcus agalactiae (23,2%) and Eschérichia coli (15,9%).
Staphylococcus aureus is the predominant bacterial pathogen in subclinical and clinical mastitis. Environmental pathogens are not to neglect in the cause of the clinical mastits.
Moreover, this study revealed the presence of antibiotic residues in 17,1% of the 35 milk samples analyzed.
The in vitro antimicrobial susceptibility of bacteria isolated from cow milk samples showed high resistance to penicillin, ampicillin, oxacillin, tetracyclin, streptomycin and erythromycin.
During the dry period, the treatment eliminates 91% of pathogenic organism. The rate of new infections prevented is 88%.Diplôme : Doctorat En ligne : ../theses/veterinaire/BOU4260.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1887 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/4260 BOU/4260 Thèse Bibliothèque principale Thèses Disponible
