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Auteur N Kacem Chaouche |
Documents disponibles écrits par cet auteur (8)
Affiner la recherche Interroger des sources externesContribution à l’étude des maladies bactériennes de la tomate (Lycopersicon esculentum Mill) cultivée en serres dans l’Est Algérien. / Zabida Hadjer Toufouti
Titre : Contribution à l’étude des maladies bactériennes de la tomate (Lycopersicon esculentum Mill) cultivée en serres dans l’Est Algérien. Type de document : texte imprimé Auteurs : Zabida Hadjer Toufouti, Auteur ; N Kacem Chaouche, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2013 Importance : 89 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Pseudomonas syringae Tomate Moucheture bactérienne Antagonisme Lutte biologique Pseudomonas syringae Tomato Bacterial Speck Antagonism Biological Control الطماطم؛اختبارات التضاد؛المحاربة البيولوجية Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Magistère En ligne : ../theses/biologie/TOU6386.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9398 Contribution à l’étude des maladies bactériennes de la tomate (Lycopersicon esculentum Mill) cultivée en serres dans l’Est Algérien. [texte imprimé] / Zabida Hadjer Toufouti, Auteur ; N Kacem Chaouche, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2013 . - 89 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Pseudomonas syringae Tomate Moucheture bactérienne Antagonisme Lutte biologique Pseudomonas syringae Tomato Bacterial Speck Antagonism Biological Control الطماطم؛اختبارات التضاد؛المحاربة البيولوجية Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Magistère En ligne : ../theses/biologie/TOU6386.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9398 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité TOU/6386 TOU/6386 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intégraleAdobe Acrobat PDF
Titre : Etude de l’activité anti-Candida albicans des microorganismes isolés à partir du sol des zones arides Type de document : texte imprimé Auteurs : Mounia Youcef-Ali, Auteur ; N Kacem Chaouche, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 209 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : "Milieux arides, Sol, Bacillus, Bacillus niabensis, Eurotium repens, Candida albicans, Activité
antifongique, Lipopeptides, Substances actives."
"Arid regions, Soil, Bacillus, Bacillus niabensis, Eurotium repens, Candida albicans,
Antifungal activity, Lipopeptides, Bioactive substances."
" ,Candida albicans , Eurotium repens ,Bacillus niabensis, Bacillus, مناطق قاحلة , تربة
مواد بيولوجية فعالة. , , lipopeptides نشاط مثبط لنمو الفطريات"Index. décimale : 570 Sciences de la vie. Biologie Résumé : "The exploration of soil samples from a palmary in the region of TOLGA, a salt lake in the
region of EL MRIERE, a salt lake of AIN M’LILA and the rhizosphere of an adjacent plant
led the obtaining of 92 bacterial and 16 fungal isolates. Three Bacillus isolates and one fungus
belonging to the genus Eurotium are screened, in vitro, for their strong antagonist effect
against Candida albicans.
Molecular identification of isolates by 16S rDNA and 18S rDNA sequences analysis for
bacteria and fungus, respectively, showed that they were closely related to Bacillus subtilis,
Bacillus mojavensis, Bacillus niabensis and Eurotium repens.
Screened bacteria develop in flasks conditions high sporulation yields varying between 8.33
% and 20 %. The halophyte character of E. repens was investigated by their capacity to grow
on salt media (80g/l for B. niabensis and 200g/l for E. repens).To our knowledge, these
obtaining data are shown for the first time in this study. The screened bacteria are able to
produce cell wall degrading enzymes (protease and cellulase), furthermore, they produce
lipopeptides on optimized medium in flasks and fermenter conditions. Interestingly, the
present work is the first to reveal the possibility of isolating B. niabensis from soil and its
ability to produce lipopeptides antifungal molecules, also. In another side, Eurotium
repens was subjected to a comparative study characterizing its growth and capacity to produce
metabolites in diverse culture conditions. In fact, E. repens develops better in salt rich
medium (3.2g/L), comparing to salt poor medium (0.36g/L). In addition, this work revealed
that metabolites produced in stress conditions differ from those produced in favorable
conditions. Isolation and identification of these metabolites by CCM, HPLC-UV, HPLC/MS
and HPLC/MS2 techniques showed the presence of tryptophan, cinnamic acid (in salt rich
medium) and oleic acid (in poor salt medium). These results are shown for the first time in
the present study."
Diplôme : Doctorat En ligne : ../theses/biologie/YOU6552.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9666 Etude de l’activité anti-Candida albicans des microorganismes isolés à partir du sol des zones arides [texte imprimé] / Mounia Youcef-Ali, Auteur ; N Kacem Chaouche, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 209 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : "Milieux arides, Sol, Bacillus, Bacillus niabensis, Eurotium repens, Candida albicans, Activité
antifongique, Lipopeptides, Substances actives."
"Arid regions, Soil, Bacillus, Bacillus niabensis, Eurotium repens, Candida albicans,
Antifungal activity, Lipopeptides, Bioactive substances."
" ,Candida albicans , Eurotium repens ,Bacillus niabensis, Bacillus, مناطق قاحلة , تربة
مواد بيولوجية فعالة. , , lipopeptides نشاط مثبط لنمو الفطريات"Index. décimale : 570 Sciences de la vie. Biologie Résumé : "The exploration of soil samples from a palmary in the region of TOLGA, a salt lake in the
region of EL MRIERE, a salt lake of AIN M’LILA and the rhizosphere of an adjacent plant
led the obtaining of 92 bacterial and 16 fungal isolates. Three Bacillus isolates and one fungus
belonging to the genus Eurotium are screened, in vitro, for their strong antagonist effect
against Candida albicans.
Molecular identification of isolates by 16S rDNA and 18S rDNA sequences analysis for
bacteria and fungus, respectively, showed that they were closely related to Bacillus subtilis,
Bacillus mojavensis, Bacillus niabensis and Eurotium repens.
Screened bacteria develop in flasks conditions high sporulation yields varying between 8.33
% and 20 %. The halophyte character of E. repens was investigated by their capacity to grow
on salt media (80g/l for B. niabensis and 200g/l for E. repens).To our knowledge, these
obtaining data are shown for the first time in this study. The screened bacteria are able to
produce cell wall degrading enzymes (protease and cellulase), furthermore, they produce
lipopeptides on optimized medium in flasks and fermenter conditions. Interestingly, the
present work is the first to reveal the possibility of isolating B. niabensis from soil and its
ability to produce lipopeptides antifungal molecules, also. In another side, Eurotium
repens was subjected to a comparative study characterizing its growth and capacity to produce
metabolites in diverse culture conditions. In fact, E. repens develops better in salt rich
medium (3.2g/L), comparing to salt poor medium (0.36g/L). In addition, this work revealed
that metabolites produced in stress conditions differ from those produced in favorable
conditions. Isolation and identification of these metabolites by CCM, HPLC-UV, HPLC/MS
and HPLC/MS2 techniques showed the presence of tryptophan, cinnamic acid (in salt rich
medium) and oleic acid (in poor salt medium). These results are shown for the first time in
the present study."
Diplôme : Doctorat En ligne : ../theses/biologie/YOU6552.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9666 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité YOU/6552 YOU/6552 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude de l’effet antimycotoxinogène et antihépatotoxicose des extraits d’une plante utilisée dans la thérapie traditionnelle. Type de document : texte imprimé Auteurs : Camelia Mosbah, Auteur ; N Kacem Chaouche, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 178 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles. Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Lycium halimifolium Mill Aspergillus flavus Aflatoxine B1 Antimycotoxinogénèse Antihépatotoxicose Aflatoxin B1 Antimycotoxinogenesis Antihepatotoxicosis Lycium halimifolium Millأفلاتوكسین B1النشاط المضاد لإنتاج
السموم النشاط المضاد للتسمم الكبديIndex. décimale : 570 Sciences de la vie. Biologie Résumé : This work deals with the antimycotoxinogenesis and antihepatotoxicosis study of Lycium halimifolium Mill.
Two fungal isolates were obtained from the peanut kernels, including a single isolate with similar characteristics to the genus Aspergillus sp. has been selected. Molecular identification confirmed that the selected isolate was an Aspergillus flavus registered under accession code (gi | 146746162 | EF409803.1) with a similarity of 99%.
The preliminary evaluation of the composition of Lycium halimifoliumMill leaves revealed the presence of several chemical groups, the alkaloids, the terpenes and the saponosides. This latter was confirmed by a quantitative analysis. The obtained results showed that ACT and BUT fractions of Lycium halimifoliumMill leaves showed considerable polyphenol contents estimated at 61.66 ± 0.042 and 60.21 ± 0.102 μg EAG / mg PS, respectively. Whereas, those of the flavonoids also showed interesting values estimated at 25.18 ± 0.014 for ACT and
17.79 ± 0.015 μg EQ / mg PS for BUT. In contrast, low tannin levels were obtained and estimated at 5.872 ± 0.053 (ACT) and 7.058 ± 0.013 μg EC / mg PS (BUT).
Chromatographic identification of the ACT fraction allowed to characterize four phenolic acids: protocatechic acid (19.29%), gallic acid (0.499%), ρ coumaric acid (0.386%), syringic acid (0.142 %) and a flavonoid (rutin) (12.58%). While the BUT fraction contains three phenolic acids: protocatechic acid (20.32%), syringic acid (0.236%), and coumaric acid (0.124%), and a flavonoid (rutin) (2.098%). It has been reported that protocatechic acid, gallic acid and syringic acid are identified for the first time in the present work on Lycium
halimifolium Mill leaves.
The antifungal activity allowed to estimate the MIC and CFS of the BUT fraction at 312 μg / ml and 625 μg / ml for the ACT fraction. The antimycotoxinogenic activity of Lycium halimifolium Mill showed an inhibition of aflatoxin B1 secreted by the BUT fraction estimated at 96.83%, and a percentage of inhibition equal to 94.99% was found by the ACT fraction. This activity was correlated with the percentage of reduction in mycelial biomass
with a biomass inhibition rate of 56.53 ± 2.74% in the presence of ACT fraction and 65.22 ± 0.10% in the presence of BUT fraction. The Evaluation of antioxidant activity of the two fractions showed that the BUT fraction was more active than ACT fraction. The Antibacterial activity revealed that the BUT fraction had a positive effect on E. coli and S. aureus.
Finally, the study of the antihepatotoxicity effect of the two fractions of Lycium halimifolium Mill leaves, showed that the mice treated with ACT and BUT experienced a normalization of the rates of TGO, TGP, PAL, as well as the standardization of the parameters of the oxidative stress. The histological study showed that both fractions preserved the hepatocytes against the oxidative lesions and thus played a protective role against oxidative stress against the histopathological changes produced by aflatoxin B1. This work is the first to have exhibited the antimycotoxinogenic activity and antihepatotoxicosis of Lycium halimifoliumMill leaves.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MOS7201.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10762 Etude de l’effet antimycotoxinogène et antihépatotoxicose des extraits d’une plante utilisée dans la thérapie traditionnelle. [texte imprimé] / Camelia Mosbah, Auteur ; N Kacem Chaouche, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 178 f. ; 30 cm.
2 copies imprimées disponibles.
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Lycium halimifolium Mill Aspergillus flavus Aflatoxine B1 Antimycotoxinogénèse Antihépatotoxicose Aflatoxin B1 Antimycotoxinogenesis Antihepatotoxicosis Lycium halimifolium Millأفلاتوكسین B1النشاط المضاد لإنتاج
السموم النشاط المضاد للتسمم الكبديIndex. décimale : 570 Sciences de la vie. Biologie Résumé : This work deals with the antimycotoxinogenesis and antihepatotoxicosis study of Lycium halimifolium Mill.
Two fungal isolates were obtained from the peanut kernels, including a single isolate with similar characteristics to the genus Aspergillus sp. has been selected. Molecular identification confirmed that the selected isolate was an Aspergillus flavus registered under accession code (gi | 146746162 | EF409803.1) with a similarity of 99%.
The preliminary evaluation of the composition of Lycium halimifoliumMill leaves revealed the presence of several chemical groups, the alkaloids, the terpenes and the saponosides. This latter was confirmed by a quantitative analysis. The obtained results showed that ACT and BUT fractions of Lycium halimifoliumMill leaves showed considerable polyphenol contents estimated at 61.66 ± 0.042 and 60.21 ± 0.102 μg EAG / mg PS, respectively. Whereas, those of the flavonoids also showed interesting values estimated at 25.18 ± 0.014 for ACT and
17.79 ± 0.015 μg EQ / mg PS for BUT. In contrast, low tannin levels were obtained and estimated at 5.872 ± 0.053 (ACT) and 7.058 ± 0.013 μg EC / mg PS (BUT).
Chromatographic identification of the ACT fraction allowed to characterize four phenolic acids: protocatechic acid (19.29%), gallic acid (0.499%), ρ coumaric acid (0.386%), syringic acid (0.142 %) and a flavonoid (rutin) (12.58%). While the BUT fraction contains three phenolic acids: protocatechic acid (20.32%), syringic acid (0.236%), and coumaric acid (0.124%), and a flavonoid (rutin) (2.098%). It has been reported that protocatechic acid, gallic acid and syringic acid are identified for the first time in the present work on Lycium
halimifolium Mill leaves.
The antifungal activity allowed to estimate the MIC and CFS of the BUT fraction at 312 μg / ml and 625 μg / ml for the ACT fraction. The antimycotoxinogenic activity of Lycium halimifolium Mill showed an inhibition of aflatoxin B1 secreted by the BUT fraction estimated at 96.83%, and a percentage of inhibition equal to 94.99% was found by the ACT fraction. This activity was correlated with the percentage of reduction in mycelial biomass
with a biomass inhibition rate of 56.53 ± 2.74% in the presence of ACT fraction and 65.22 ± 0.10% in the presence of BUT fraction. The Evaluation of antioxidant activity of the two fractions showed that the BUT fraction was more active than ACT fraction. The Antibacterial activity revealed that the BUT fraction had a positive effect on E. coli and S. aureus.
Finally, the study of the antihepatotoxicity effect of the two fractions of Lycium halimifolium Mill leaves, showed that the mice treated with ACT and BUT experienced a normalization of the rates of TGO, TGP, PAL, as well as the standardization of the parameters of the oxidative stress. The histological study showed that both fractions preserved the hepatocytes against the oxidative lesions and thus played a protective role against oxidative stress against the histopathological changes produced by aflatoxin B1. This work is the first to have exhibited the antimycotoxinogenic activity and antihepatotoxicosis of Lycium halimifoliumMill leaves.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MOS7201.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10762 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MOS/7201 MOS/7201 Thèse Bibliothèque principale Thèses Disponible
Titre : Isolement et caractérisation de souches levuriennes des milieux arides productrices de l’éthanol sur différents substrats Type de document : texte imprimé Auteurs : Mounira KaraAli, Auteur ; N Kacem Chaouche, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : : Ethanol, Pichia caribbica, Inuline, Inulinase, Fructose, Sol des régions arides
Ethanol, Pichia caribbica, Inuline, Inulinase, Fructose, arid region soils
الإيثانول ، Pichia Caribbica ، الإينيلين ، الإينيليناز ،الفريكتوز ، تربة أراضي قاحلة.Index. décimale : 570 Sciences de la vie. Biologie Résumé : The exploration of soil samples taken from the palmary of Tolga region (North West province of Biskra, Algeria) allowed the obtaining of nine yeast strains. One of the isolates (L5) was selected for its ability to produce ethanol from inulin as a sole carbon source. Preliminary identification of the isolate (L5) based on macroscopic, microscopic and biochemical study, using the API gallery API AUX 20, revealed its membership to Pichia genus. The molecular identification using 18S- DNA and ITS analysis showed that L5 is closely related to Pichia caribbica, registered under accession number; KC977491. The degradation of inulin to produce ethanol by P. caribbica is performed in two steps, namely: the saccharification of inulin to fructose and fermentation this later to ethanol. Under optimal conditions of ethanol production, i.e., 40 g /L of inulin, pH 5 and 37 ° C, P. caribbica produced in flasks conditions, after 72 h of culture, 12.6 g / L ethanol, recording a yield of 0.31 g ethanol / g of inulin. However, this strain has the ability to produce 14 g / L of ethanol in a 20 liter fermentor, reaching a yield of 0.35 g ethanol / g inulin. The results of inulinase secretion kinetics (the enzyme that catalyzes the degradation of inulin) by P. caribbica revealed that this strain is able of producing important quantities of inulinase, reaching 54.27 IU / mL in flasks and 55.47 IU / mL in 20 L fermentor, this after 96 h of culture. The partial characterization of this enzyme revealed that its maximum activity of (108.72 IU / mL) is stored at 55 ° C and pH 3.4 and greater thermostability at the same temperature (55 ° C) after one hour of reaction. The use of artichoke as a sole source of carbon and nitrogen for the production of ethanol by P. caribbica allowed the obtaining of a considerable quantity of ethanol exceeding 14g / L, which opens now, the way to the industrial exploitation of this agricultural product. Mathematical models corresponding to biotechnological fermentation process have been implemented to simulate the fermentation phenomenon. Simulation results allow the understanding of substrate degradation, the growth and ethanol production. For an appropriate choice of model parameters, a good qualitative correspondence is noted for the model profiles obtained by our simulation compared to experimental results.
Diplôme : Doctorat En ligne : ../theses/biologie/KAR6538.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9653 Isolement et caractérisation de souches levuriennes des milieux arides productrices de l’éthanol sur différents substrats [texte imprimé] / Mounira KaraAli, Auteur ; N Kacem Chaouche, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : : Ethanol, Pichia caribbica, Inuline, Inulinase, Fructose, Sol des régions arides
Ethanol, Pichia caribbica, Inuline, Inulinase, Fructose, arid region soils
الإيثانول ، Pichia Caribbica ، الإينيلين ، الإينيليناز ،الفريكتوز ، تربة أراضي قاحلة.Index. décimale : 570 Sciences de la vie. Biologie Résumé : The exploration of soil samples taken from the palmary of Tolga region (North West province of Biskra, Algeria) allowed the obtaining of nine yeast strains. One of the isolates (L5) was selected for its ability to produce ethanol from inulin as a sole carbon source. Preliminary identification of the isolate (L5) based on macroscopic, microscopic and biochemical study, using the API gallery API AUX 20, revealed its membership to Pichia genus. The molecular identification using 18S- DNA and ITS analysis showed that L5 is closely related to Pichia caribbica, registered under accession number; KC977491. The degradation of inulin to produce ethanol by P. caribbica is performed in two steps, namely: the saccharification of inulin to fructose and fermentation this later to ethanol. Under optimal conditions of ethanol production, i.e., 40 g /L of inulin, pH 5 and 37 ° C, P. caribbica produced in flasks conditions, after 72 h of culture, 12.6 g / L ethanol, recording a yield of 0.31 g ethanol / g of inulin. However, this strain has the ability to produce 14 g / L of ethanol in a 20 liter fermentor, reaching a yield of 0.35 g ethanol / g inulin. The results of inulinase secretion kinetics (the enzyme that catalyzes the degradation of inulin) by P. caribbica revealed that this strain is able of producing important quantities of inulinase, reaching 54.27 IU / mL in flasks and 55.47 IU / mL in 20 L fermentor, this after 96 h of culture. The partial characterization of this enzyme revealed that its maximum activity of (108.72 IU / mL) is stored at 55 ° C and pH 3.4 and greater thermostability at the same temperature (55 ° C) after one hour of reaction. The use of artichoke as a sole source of carbon and nitrogen for the production of ethanol by P. caribbica allowed the obtaining of a considerable quantity of ethanol exceeding 14g / L, which opens now, the way to the industrial exploitation of this agricultural product. Mathematical models corresponding to biotechnological fermentation process have been implemented to simulate the fermentation phenomenon. Simulation results allow the understanding of substrate degradation, the growth and ethanol production. For an appropriate choice of model parameters, a good qualitative correspondence is noted for the model profiles obtained by our simulation compared to experimental results.
Diplôme : Doctorat En ligne : ../theses/biologie/KAR6538.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9653 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KAR/6538 KAR/6538 Thèse Bibliothèque principale Thèses Disponible
Titre : Isolement de microorganismes à partir du sol des régions arides et sélection d’isolats à effet antagoniste sur l’agent de l’Alternariose Type de document : texte imprimé Auteurs : Asma Milet, Auteur ; N Kacem Chaouche, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 122 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Tomate Alternariose Alternaria alternata Bacillus Providencia vermicola lipopeptides Cymomètre à flux biocontrôle Tomato early blight Flow cytometer biocontrol لطماطم اللفحة المبكرة تقنية تدفق الكريات والمكافحة
البيولوجيةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Early blight is one of the most important diseases of tomato in eastern Algeria. Tomato
samples exploration (leaves, stems and fruits) with symptoms of early blight, grown under
glass in Hamma Bouziane locality (Constantine), allowed obtaining a filamentous fungal
isolate (Alt 1). The morphological and molecular identification (DNA-ITS analysis) of this
isolate revealed that it is Alternaria alternata (HQ846574.1). Pathogenicity testing of
seedlings by isolate resulted in the challenge of the typical early blight disease with a severity
of ≥ 76%. Samples exploration from various environments in the Algerian Sahara (Biskra and
Tougourt) resulted in the obtaining of 45 microbial isolates capable of developing an effect on
early blight agent. The selection test showed that; E1B3, S5 (isolated from Biskra) and E2X1,
S1.1 (isolated from Tougourt) possess a considerable antifungal effect against A. alternata
(inhibition rates vary between 60% and 75%), however, the E1B3 isolate developed the best
effect. The morphological and molecular identification of the selected isolates by analysis of
the 16S DNA and the "gyrase-A" gene showed that the bacterial isolates; E1B3, S5, and S1.1,
are identified as Bacillus mojavensis (KC977492), Bacillus amyloliquefaciens (KC977493),
and Bacillus Subtilis (KP699114.1) respectively, whereas the bacterial isolate E2X1 is
identified as Providencia vermicola (KP059130.1). To the best of our knowledge, the
isolation of P. vermicola from the palmerais rhizospheric soil and the demonstration of its
antifungal potential are established for the first time in this work.
The in vivo tests that carried out in pots and field conditions on tomato showed different
effects according to the bacterial species, and B. mojavensis developed the best effect
considering the most studied parameters (disease severity, Seedling size, leaves number and
flowers number per seedling). The P. vermicola strain showed the greatest result on the
severity reduction of early blight disease, indicating that the present work is the first one
which revealed this effect. In contrast, B. mojavensis developed the best protective effect,
considering the majority of the parameters studied in vitro and in vivo. This impact is due to
the ability of this strain to produce antifungal substances in this case; the enzymes (lipase and
protease) and the three families of lipopeptides (iturin, surfactins, and fengycins). The
evaluation of the antagonistic effect developed by B. mojavenssvis versus A. alternata through
flow cytometer (FCM) showed a considerable decrease in the rate of A. Alternata living cells,
confirming the inhibitory effect of B. mojavensis against the pathogenic agent of early blight.Diplôme : Doctorat En ligne : ../theses/biologie/MIL7125.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10477 Isolement de microorganismes à partir du sol des régions arides et sélection d’isolats à effet antagoniste sur l’agent de l’Alternariose [texte imprimé] / Asma Milet, Auteur ; N Kacem Chaouche, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 122 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Tomate Alternariose Alternaria alternata Bacillus Providencia vermicola lipopeptides Cymomètre à flux biocontrôle Tomato early blight Flow cytometer biocontrol لطماطم اللفحة المبكرة تقنية تدفق الكريات والمكافحة
البيولوجيةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Early blight is one of the most important diseases of tomato in eastern Algeria. Tomato
samples exploration (leaves, stems and fruits) with symptoms of early blight, grown under
glass in Hamma Bouziane locality (Constantine), allowed obtaining a filamentous fungal
isolate (Alt 1). The morphological and molecular identification (DNA-ITS analysis) of this
isolate revealed that it is Alternaria alternata (HQ846574.1). Pathogenicity testing of
seedlings by isolate resulted in the challenge of the typical early blight disease with a severity
of ≥ 76%. Samples exploration from various environments in the Algerian Sahara (Biskra and
Tougourt) resulted in the obtaining of 45 microbial isolates capable of developing an effect on
early blight agent. The selection test showed that; E1B3, S5 (isolated from Biskra) and E2X1,
S1.1 (isolated from Tougourt) possess a considerable antifungal effect against A. alternata
(inhibition rates vary between 60% and 75%), however, the E1B3 isolate developed the best
effect. The morphological and molecular identification of the selected isolates by analysis of
the 16S DNA and the "gyrase-A" gene showed that the bacterial isolates; E1B3, S5, and S1.1,
are identified as Bacillus mojavensis (KC977492), Bacillus amyloliquefaciens (KC977493),
and Bacillus Subtilis (KP699114.1) respectively, whereas the bacterial isolate E2X1 is
identified as Providencia vermicola (KP059130.1). To the best of our knowledge, the
isolation of P. vermicola from the palmerais rhizospheric soil and the demonstration of its
antifungal potential are established for the first time in this work.
The in vivo tests that carried out in pots and field conditions on tomato showed different
effects according to the bacterial species, and B. mojavensis developed the best effect
considering the most studied parameters (disease severity, Seedling size, leaves number and
flowers number per seedling). The P. vermicola strain showed the greatest result on the
severity reduction of early blight disease, indicating that the present work is the first one
which revealed this effect. In contrast, B. mojavensis developed the best protective effect,
considering the majority of the parameters studied in vitro and in vivo. This impact is due to
the ability of this strain to produce antifungal substances in this case; the enzymes (lipase and
protease) and the three families of lipopeptides (iturin, surfactins, and fengycins). The
evaluation of the antagonistic effect developed by B. mojavenssvis versus A. alternata through
flow cytometer (FCM) showed a considerable decrease in the rate of A. Alternata living cells,
confirming the inhibitory effect of B. mojavensis against the pathogenic agent of early blight.Diplôme : Doctorat En ligne : ../theses/biologie/MIL7125.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10477 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MIL/7125 MIL/7125 Thèse Bibliothèque principale Thèses Disponible
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