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Titre : Criblage des souches d’actinobactéries pour leurs pouvoirs PGPR : Essais in vitro et in planta sur Solanum lycopersicum L., Triticum durum Desf., et Daucus carota L. Type de document : texte imprimé Auteurs : Rihab Djebaili, Auteur ; Mahmoud Kitouni, Directeur de thèse ; Maddalena Del Gallo, Directeur de thèse Mention d'édition : 11-11-2021 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2021 Importance : 244 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Microbiologie: Microbiologie générale et appliquée PGPB actinomycètes solubilisation du phosphate acide indole-3-acétique acide cyanhydrique biofertilisant ACC désaminase blé dur stress salin halotolérance la lutte biologique pathogènes SEM filtrats de culture tomate carotte actinomycetes phosphatesolubilization indole-3-acetic acid hydrocyanic acid biofertilizer ACC deaminase durum wheat salt stress halotolerance biological control pathogens culture filtrates tomato carrot Index. décimale : 570 Sciences de la vie. Biologie Résumé : Abiotic stress due to climate change and phytopathogens attacks lead to several damages in agricultural crops. Excessive use of chemicals in agriculture causes environmental pollution and loss of agricultural lands with fertile soils. Plant growth-promoting bacteria (PGPB) are an alternative strategy for sustainable agriculture. Among PGPBs, actinomycetes have recently gained increasing attention for their ability to alleviate plant stress and improve agricultural productivity. This study aimed to select actinomycetes strains with plant growth promoting traits, their plantroots association capacities, and their biostimulating effects without and with salt stress as well as the biocontrol power against several fungal and bacterial plant pathogens. The strains were studied for their phosphate solubilization capacity, indole-3-acetic acid (IAA), hydrocyanic acid (HCN),
and ammonia (NH3) production, and several enzymatic activities. Bacteria-root associations were studied by scanning electron microscopy (SEM), and a greenhouse experiment was performed to assess the inoculation effects on Solanum lycopersiumL.. Likewise, these strains were tested for in vitro halotolerant ability by several PGP tests with different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl) and by a greenhouse experiment on Triticum durum. The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also studied. Moreover, the strains were screened for their in vitro biocontrol activity by soluble and volatile compounds production against the fungal strains Fusarium oxysporum f. sp. radicis-lycopersici (FORL) and Rhizoctonia solani (RHS). The post-interaction events fungus-PGPB were analyzed by scanning electron microscopy. The antibacterial activity was evaluated against Pseudomonassyringae, Pseudomonas corrugata, Pseudomonas syringaepv. actinidiae, and Pectobacteriumcarotovorum subsp. carotovorum. Strains showing good in vitro biological control activities were studied for their antifungal and antibacterial activity using the cell culture filtrate (CFS). The consortium of potent strain was applied on Solanum lycopersicum L. and Daucus carota L. for in planta biocontrol activity. Among sixty actinomycetes, fourteen strains exhibited plant growth promoting characteristics. These strains were able to solubilize the phosphate, produce ammonia, and showed several enzymatic activities at different rates. The scanning electron microscopy revealed a good in vitro plant root association and colonization abilities. The actinomycetes inoculation influenced positively the tomato growth parameters. These strains exhibited important in vitro PGP traits under different salt concentrations. Most strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity with significant amounts of ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strainsimproved the durum wheat morpho-biochemical parameters, which recorded a significantly higher content of chlorophyll and proline than those of uninoculated controls both under normal and stressed conditions. Strains H12 (Streptomyces albidoflavus) and H14 (Nocardiopsisaegyptica) showed good in vitro antifungal activity with diffusible and volatile compounds (inhibition up to 85%) and antibacterial activity (diameter of inhibition> 10 mm). SEM micrographs showed morphological deterioration of the fungal filaments and changes in their structures. The CFS of potent strains was also active in fungal and bacterial pathogens inhibition (minimum inhibitory concentration up to 0.2%). The consortium was also able to alleviate the infection symptoms and allow a normal growth of the infected plants compared to the control. The obtained results demonstrate the efficacy of halotolerant actinomycetes strains as a biofertilizer and biocontrol agent for plant diseases management and for the alleviation of negative effects of salt stress in plants.
Note de contenu : Annexes.
Diplôme : Doctorat En ligne : ../theses/biologie/DJE7836.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11668 Criblage des souches d’actinobactéries pour leurs pouvoirs PGPR : Essais in vitro et in planta sur Solanum lycopersicum L., Triticum durum Desf., et Daucus carota L. [texte imprimé] / Rihab Djebaili, Auteur ; Mahmoud Kitouni, Directeur de thèse ; Maddalena Del Gallo, Directeur de thèse . - 11-11-2021 . - جامعة الإخوة منتوري قسنطينة, 2021 . - 244 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Microbiologie: Microbiologie générale et appliquée PGPB actinomycètes solubilisation du phosphate acide indole-3-acétique acide cyanhydrique biofertilisant ACC désaminase blé dur stress salin halotolérance la lutte biologique pathogènes SEM filtrats de culture tomate carotte actinomycetes phosphatesolubilization indole-3-acetic acid hydrocyanic acid biofertilizer ACC deaminase durum wheat salt stress halotolerance biological control pathogens culture filtrates tomato carrot Index. décimale : 570 Sciences de la vie. Biologie Résumé : Abiotic stress due to climate change and phytopathogens attacks lead to several damages in agricultural crops. Excessive use of chemicals in agriculture causes environmental pollution and loss of agricultural lands with fertile soils. Plant growth-promoting bacteria (PGPB) are an alternative strategy for sustainable agriculture. Among PGPBs, actinomycetes have recently gained increasing attention for their ability to alleviate plant stress and improve agricultural productivity. This study aimed to select actinomycetes strains with plant growth promoting traits, their plantroots association capacities, and their biostimulating effects without and with salt stress as well as the biocontrol power against several fungal and bacterial plant pathogens. The strains were studied for their phosphate solubilization capacity, indole-3-acetic acid (IAA), hydrocyanic acid (HCN),
and ammonia (NH3) production, and several enzymatic activities. Bacteria-root associations were studied by scanning electron microscopy (SEM), and a greenhouse experiment was performed to assess the inoculation effects on Solanum lycopersiumL.. Likewise, these strains were tested for in vitro halotolerant ability by several PGP tests with different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl) and by a greenhouse experiment on Triticum durum. The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also studied. Moreover, the strains were screened for their in vitro biocontrol activity by soluble and volatile compounds production against the fungal strains Fusarium oxysporum f. sp. radicis-lycopersici (FORL) and Rhizoctonia solani (RHS). The post-interaction events fungus-PGPB were analyzed by scanning electron microscopy. The antibacterial activity was evaluated against Pseudomonassyringae, Pseudomonas corrugata, Pseudomonas syringaepv. actinidiae, and Pectobacteriumcarotovorum subsp. carotovorum. Strains showing good in vitro biological control activities were studied for their antifungal and antibacterial activity using the cell culture filtrate (CFS). The consortium of potent strain was applied on Solanum lycopersicum L. and Daucus carota L. for in planta biocontrol activity. Among sixty actinomycetes, fourteen strains exhibited plant growth promoting characteristics. These strains were able to solubilize the phosphate, produce ammonia, and showed several enzymatic activities at different rates. The scanning electron microscopy revealed a good in vitro plant root association and colonization abilities. The actinomycetes inoculation influenced positively the tomato growth parameters. These strains exhibited important in vitro PGP traits under different salt concentrations. Most strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity with significant amounts of ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strainsimproved the durum wheat morpho-biochemical parameters, which recorded a significantly higher content of chlorophyll and proline than those of uninoculated controls both under normal and stressed conditions. Strains H12 (Streptomyces albidoflavus) and H14 (Nocardiopsisaegyptica) showed good in vitro antifungal activity with diffusible and volatile compounds (inhibition up to 85%) and antibacterial activity (diameter of inhibition> 10 mm). SEM micrographs showed morphological deterioration of the fungal filaments and changes in their structures. The CFS of potent strains was also active in fungal and bacterial pathogens inhibition (minimum inhibitory concentration up to 0.2%). The consortium was also able to alleviate the infection symptoms and allow a normal growth of the infected plants compared to the control. The obtained results demonstrate the efficacy of halotolerant actinomycetes strains as a biofertilizer and biocontrol agent for plant diseases management and for the alleviation of negative effects of salt stress in plants.
Note de contenu : Annexes.
Diplôme : Doctorat En ligne : ../theses/biologie/DJE7836.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11668 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité DJE/7836 DJE/7836 Thèse Bibliothèque principale Thèses Disponible
Titre : Isolement des microorganismes possédant une activité anti- Fusarium Type de document : texte imprimé Auteurs : Sana Ghorri, Auteur ; L. Dehimat, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2015 Importance : 140 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Fusarium Fusariose lutte biologique Trichoderma BIOLOG extraits fongiques biological control fungal extracts المكافحة البيولوجيةة المستخلصات الفطرية Index. décimale : 570 Sciences de la vie. Biologie Résumé : This study was carried out in order to fight against the phytopathogenic fungus ""Fusarium"".
eight species from the genus Fusarium have been isolated from different organs of infected
plants. genus identification was conducted using morphological characters, while the
identification of species was performed by molecular pathway within the LMBA Laboratory
(Laboratory of Microbiology and Biomolecules Assets).
Indeed, according to affiliation results, the eight pathogenic strains belong to three Fusarium
species in the circumstances; Fusarium verticillioides, Fusarium solani and Fusarium
oxysporum.
Biological control against the phytopathogens is highlighted using seven strains from the
genus Trichoderma isolated from three Algerian agricultural soils.
The molecular identification of isolats conducted in the laboratory of LMBA has divided
the seven strains into five main species: Trichoderma album, Trichoderma harzianum,
Trichoderma virens, Trichoderma longibrachiatum, Trichoderma viride. Indeed, This study
showed a significant diversity of Trichoderma species in Algeria. However, to our
knowledge, there are no previous published studies on the isolation of Trichoderma album,
Trichoderma virens and Trichoderma longibrachiatum, from the Algerian soil.
The effect of the antagonist activity of Trichoderma strains against the eight isolated
Fusarium species was studied using two methods, direct and indirect confrontation. Direct
confrontation results show a percentage of inhibition ranging from 55% to 72% depending on
the tested pathogenic and antagonists species, while the results of the confrontation from
distance reveals low percentage of inhibition varied from 2% to 43%.
The seven tested Trichoderma strains show considerable capacity of resistance on the media
with a different pH from 2 to 9. Enzymatic activity test of antagonistic strains shows that the
seven tested strains represent very important sources of lytic enzymes namely chitinase,
cellulase, protease, lipase and laccase.
Both test for anti-Fusarium activity, and culture filtrates antagonistic strains show a low
inhibition of mycelial growth of pathogenic strains. Furthermore, this study showed a
remarkable change of color and appearance of the Fusarium strains due to antagonistic
metabolits.
chloroformic extract effect of the seven antagonistic strains, was tested by the BIOLOG
technic and by the technical soaked disks, both techniques show a good inhibitory activity of
the extracts, no matter what the concentration used is (50 mg / ml , 25 mg / ml and 1mg / ml)
Diplôme : Doctorat En ligne : ../theses/biologie/GHO6792.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10015 Isolement des microorganismes possédant une activité anti- Fusarium [texte imprimé] / Sana Ghorri, Auteur ; L. Dehimat, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2015 . - 140 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Fusarium Fusariose lutte biologique Trichoderma BIOLOG extraits fongiques biological control fungal extracts المكافحة البيولوجيةة المستخلصات الفطرية Index. décimale : 570 Sciences de la vie. Biologie Résumé : This study was carried out in order to fight against the phytopathogenic fungus ""Fusarium"".
eight species from the genus Fusarium have been isolated from different organs of infected
plants. genus identification was conducted using morphological characters, while the
identification of species was performed by molecular pathway within the LMBA Laboratory
(Laboratory of Microbiology and Biomolecules Assets).
Indeed, according to affiliation results, the eight pathogenic strains belong to three Fusarium
species in the circumstances; Fusarium verticillioides, Fusarium solani and Fusarium
oxysporum.
Biological control against the phytopathogens is highlighted using seven strains from the
genus Trichoderma isolated from three Algerian agricultural soils.
The molecular identification of isolats conducted in the laboratory of LMBA has divided
the seven strains into five main species: Trichoderma album, Trichoderma harzianum,
Trichoderma virens, Trichoderma longibrachiatum, Trichoderma viride. Indeed, This study
showed a significant diversity of Trichoderma species in Algeria. However, to our
knowledge, there are no previous published studies on the isolation of Trichoderma album,
Trichoderma virens and Trichoderma longibrachiatum, from the Algerian soil.
The effect of the antagonist activity of Trichoderma strains against the eight isolated
Fusarium species was studied using two methods, direct and indirect confrontation. Direct
confrontation results show a percentage of inhibition ranging from 55% to 72% depending on
the tested pathogenic and antagonists species, while the results of the confrontation from
distance reveals low percentage of inhibition varied from 2% to 43%.
The seven tested Trichoderma strains show considerable capacity of resistance on the media
with a different pH from 2 to 9. Enzymatic activity test of antagonistic strains shows that the
seven tested strains represent very important sources of lytic enzymes namely chitinase,
cellulase, protease, lipase and laccase.
Both test for anti-Fusarium activity, and culture filtrates antagonistic strains show a low
inhibition of mycelial growth of pathogenic strains. Furthermore, this study showed a
remarkable change of color and appearance of the Fusarium strains due to antagonistic
metabolits.
chloroformic extract effect of the seven antagonistic strains, was tested by the BIOLOG
technic and by the technical soaked disks, both techniques show a good inhibitory activity of
the extracts, no matter what the concentration used is (50 mg / ml , 25 mg / ml and 1mg / ml)
Diplôme : Doctorat En ligne : ../theses/biologie/GHO6792.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10015 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité GHO/6792 GHO/6792 Thèse Bibliothèque principale Thèses Disponible Contribution à l’étude des maladies bactériennes de la tomate (Lycopersicon esculentum Mill) cultivée en serres dans l’Est Algérien. / Zabida Hadjer Toufouti
Titre : Contribution à l’étude des maladies bactériennes de la tomate (Lycopersicon esculentum Mill) cultivée en serres dans l’Est Algérien. Type de document : texte imprimé Auteurs : Zabida Hadjer Toufouti, Auteur ; N Kacem Chaouche, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2013 Importance : 89 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Pseudomonas syringae Tomate Moucheture bactérienne Antagonisme Lutte biologique Pseudomonas syringae Tomato Bacterial Speck Antagonism Biological Control الطماطم؛اختبارات التضاد؛المحاربة البيولوجية Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Magistère En ligne : ../theses/biologie/TOU6386.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9398 Contribution à l’étude des maladies bactériennes de la tomate (Lycopersicon esculentum Mill) cultivée en serres dans l’Est Algérien. [texte imprimé] / Zabida Hadjer Toufouti, Auteur ; N Kacem Chaouche, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2013 . - 89 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Pseudomonas syringae Tomate Moucheture bactérienne Antagonisme Lutte biologique Pseudomonas syringae Tomato Bacterial Speck Antagonism Biological Control الطماطم؛اختبارات التضاد؛المحاربة البيولوجية Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Magistère En ligne : ../theses/biologie/TOU6386.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9398 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité TOU/6386 TOU/6386 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intégraleAdobe Acrobat PDF
Titre : دراسة تأثیر المكافحة البیولوجیة بفطر Trichoderma harzianum على بعض الفطریات المصاحبة داخلیا لبذور بعضأصناف القمح لصلب Triticumdurum Desf Type de document : texte imprimé Auteurs : مختار حمیتو, Auteur ; العيد دھيمات, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 101 ورقة. Format : 30 سم. Note générale : 2نسخ موجودة في المكتبة المركزي Langues : Arabe (ara) Catégories : Arabe
5 البيولوجياTags : Trichoderma harzianum Triticum durum potentiel antagoniste lutte biologique antagonism capability biological control قدرة التضادیة المكافحة البیولوجیة Index. décimale : 570 بيولوجيا Résumé : The present investigation aimed to isolate and to identify the mycetes accompanying interior in durum wheat seed (Triticum durum Desf.) and to evaluate the in vitro antagonism capability of Trichoderma harzianum against the isolated fungi. Seed samples of durum wheat cultivars
Vitron, Waha, GTA Dur and Cirta were made available by the cereal and dry legumes cooperative of Oum-El-Bouaghi (Algeria). First year results allowed the identification of 93 isolates from GTA Dur, Waha and Vitron genotypes. Most frequents genus were, in that order,
Alternaria, Cladosporium and Fusarium. 83 isolates were identified in second year from Waha, Vitron and Cirta from which Alternaria were the most frequent genera and Fusarium acuminatum. 129 isolates were found the third year from GTA Dur Waha, Vitron and Cirta, with
Alternaria, as the most frequent genera, Fusarium, Stemphylium botryosum, Cladosporium sp , and Botrytis cinerea. The results of the in vitro study of the antagonistic ability of T.harzianum,against the isolated fungi, indicated the inhibition of mycelium growth to variable degrees.
Microscopic observations showed that T.harzianum induced cell lyse, destroyed mycelia and spores of the tested isolates, excepted those belonging to Fusarium genus. T.harzianum produced haustoria on mycelia of tested isolates through mycoparasitism. The results of the in vitro study of remote confrontation of T.harzianum with the tested isolates, on PDA medium, suggested that volatile metabolic substances of T.harzianum reduced the growth of the various pathogenic mycetes. Microscopic observations of mycelia showed that, the volatile metabolic substances of T.harzianum induced degradation and inhibited sporulation of certain isolates compared to the non-treated checks. Seed treatment of the studied durum varieties with T.harzianum filtrate enhanced seed germination ability and stimulated seedling growth compared to untreated seed which exhibited weak growth and presented malformations. Treated seed were less infected by
the mycetesDiplôme : Doctorat en sciences En ligne : ../theses/biologie/AHAM3966.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9546 دراسة تأثیر المكافحة البیولوجیة بفطر Trichoderma harzianum على بعض الفطریات المصاحبة داخلیا لبذور بعضأصناف القمح لصلب Triticumdurum Desf [texte imprimé] / مختار حمیتو, Auteur ; العيد دھيمات, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 101 ورقة. ; 30 سم.
2نسخ موجودة في المكتبة المركزي
Langues : Arabe (ara)
Catégories : Arabe
5 البيولوجياTags : Trichoderma harzianum Triticum durum potentiel antagoniste lutte biologique antagonism capability biological control قدرة التضادیة المكافحة البیولوجیة Index. décimale : 570 بيولوجيا Résumé : The present investigation aimed to isolate and to identify the mycetes accompanying interior in durum wheat seed (Triticum durum Desf.) and to evaluate the in vitro antagonism capability of Trichoderma harzianum against the isolated fungi. Seed samples of durum wheat cultivars
Vitron, Waha, GTA Dur and Cirta were made available by the cereal and dry legumes cooperative of Oum-El-Bouaghi (Algeria). First year results allowed the identification of 93 isolates from GTA Dur, Waha and Vitron genotypes. Most frequents genus were, in that order,
Alternaria, Cladosporium and Fusarium. 83 isolates were identified in second year from Waha, Vitron and Cirta from which Alternaria were the most frequent genera and Fusarium acuminatum. 129 isolates were found the third year from GTA Dur Waha, Vitron and Cirta, with
Alternaria, as the most frequent genera, Fusarium, Stemphylium botryosum, Cladosporium sp , and Botrytis cinerea. The results of the in vitro study of the antagonistic ability of T.harzianum,against the isolated fungi, indicated the inhibition of mycelium growth to variable degrees.
Microscopic observations showed that T.harzianum induced cell lyse, destroyed mycelia and spores of the tested isolates, excepted those belonging to Fusarium genus. T.harzianum produced haustoria on mycelia of tested isolates through mycoparasitism. The results of the in vitro study of remote confrontation of T.harzianum with the tested isolates, on PDA medium, suggested that volatile metabolic substances of T.harzianum reduced the growth of the various pathogenic mycetes. Microscopic observations of mycelia showed that, the volatile metabolic substances of T.harzianum induced degradation and inhibited sporulation of certain isolates compared to the non-treated checks. Seed treatment of the studied durum varieties with T.harzianum filtrate enhanced seed germination ability and stimulated seedling growth compared to untreated seed which exhibited weak growth and presented malformations. Treated seed were less infected by
the mycetesDiplôme : Doctorat en sciences En ligne : ../theses/biologie/AHAM3966.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9546 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité حمي/3966 حمي/3966 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intégraleAdobe Acrobat PDF
