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Selection In vitro pour la tolerance aux stress salin et thermique chez le ble tendre (Triticum aestivum l.) / Laid Benderradji
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Titre : Selection In vitro pour la tolerance aux stress salin et thermique chez le ble tendre (Triticum aestivum l.) Type de document : texte imprimé Auteurs : Laid Benderradji, Auteur ; Hamenna Bouzerzour, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2013 Importance : 129 f. Format : 30 cm Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Triticum aestivum L. stress salin stress thermique germination surface foliaire embryons matures régénération sélection in vitro transporteurs d’ions expression génique القمح اللين الإجھاد الملحي الإجھاد الحراري، الانتاش المساحة الورقية أجنة ناضجة التشكل النباتي الانتخاب ألمخبري نواقل الايونات الترجمة الجينية salt stress heat stress leaf area mature
embryos regeneration in vitro selection ion transport gene expressionIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Study results of response for seedlings of two bread wheat varieties ( Triticum aestivum L.) , Mahon-Demias (MD) and Hidhab (HD) to salt stress, indicate the highlight negative impact of salinity on the rate of germination and show the sensitivity of MD compared to HD.
Salt stress significantly reduced the length of roots and epicotyls of both varieties. MD showed a greater reduction in length of two characters than HD. Under salt stress of 200mM NaCl, the leaf area is reduced by 50.2 % for MD against 24.8 % for HD. Changes in water potential values and relative water content indicates that MD is more sensitive to salinity than HD. Monitoring the ionic content indicates that the accumulation of Na + is higher in the sheath of HD and the blade of MD, with little difference between varieties for the content of the roots. HD uses an additional mechanism to counter salinity, sequestering preferentially over sodium in the sheath unlike in the blade of MD variety. HD has a greater ability to accumulate K + ion in the limb and in the roots compared to MD. The K +/Na + ratio of leaf blade is higher in HD compared to MD. This indicates that it is mainly their leaves HD restores the balance of the osmotic potential via K+ and thus minimizes the effect of osmotic stress caused by NaCl. HD capacity to pump K + in the nutrient solution, and then to transfer to the limb, therefore a strategy for adaptation to salt stress via osmotic regulation. Results of study for the embryogenic capacity and power regeneration in vitro thermal and salt stress in MD and HD using mature embryos as explants show that MD responds better than HD in vitro selection. MD has a higher rate of induction of callus with a better callus induced
proliferation, but with fewer embryos induced by cal. The rate of regeneration of plantlets from proliferating callus was null for HD and very low for MD. The index sensitivity (IS)confirmed the tolerance of MD compared to HD for salt and thermal stress in vitro to a given parameter is the length of the roots. The results suggest the possibility of using the in vitro technique in selection for no biotic stress. Results of study for the expression analysis of five candidate genes for tolerance to salinity indicate that HKT1,5 and HKT2,2 are expressed in the roots, but not in sheaths and limbs, with better HKT1,5 gene expression in the roots of HD. This suggests a more active role HKT1, 5 genes in HD. The expression of anti-porter vacuolar Na +/H+, TNHX-1 roots, sheaths and limbs is higher in HD than in MD. Roots and sheath of the two genotypes accumulate more transcripts of the vacuolar pyrophosphatase
TVP1 than blades. Type similarity of gene expression TNHX1 and TVP1, observed in MD and HD, suggesting that vacuolar compartmentalization is equal efficiency in both genotypes.
More gene transcripts TaSOS-1, accumulate in the roots and sheath of MD compared to HD, suggesting that in addition to more effective retention of Na + in sheaths, HD avoids accumulation of Na + in the blade by activating its sodium efflux via high expression of SOS1 gene in this compartment. Results indicate that salinity tolerance in the bread wheat seems to be related to the ability to prevent the accumulation of toxic levels of Na +, associated with a high osmo-regulation and/or ability to maintain an acceptable level of K +, especially in the leaf blade. This information is useful for selection of plant material in cereal breeding programs.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BEN6415.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9435 Selection In vitro pour la tolerance aux stress salin et thermique chez le ble tendre (Triticum aestivum l.) [texte imprimé] / Laid Benderradji, Auteur ; Hamenna Bouzerzour, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2013 . - 129 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Triticum aestivum L. stress salin stress thermique germination surface foliaire embryons matures régénération sélection in vitro transporteurs d’ions expression génique القمح اللين الإجھاد الملحي الإجھاد الحراري، الانتاش المساحة الورقية أجنة ناضجة التشكل النباتي الانتخاب ألمخبري نواقل الايونات الترجمة الجينية salt stress heat stress leaf area mature
embryos regeneration in vitro selection ion transport gene expressionIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Study results of response for seedlings of two bread wheat varieties ( Triticum aestivum L.) , Mahon-Demias (MD) and Hidhab (HD) to salt stress, indicate the highlight negative impact of salinity on the rate of germination and show the sensitivity of MD compared to HD.
Salt stress significantly reduced the length of roots and epicotyls of both varieties. MD showed a greater reduction in length of two characters than HD. Under salt stress of 200mM NaCl, the leaf area is reduced by 50.2 % for MD against 24.8 % for HD. Changes in water potential values and relative water content indicates that MD is more sensitive to salinity than HD. Monitoring the ionic content indicates that the accumulation of Na + is higher in the sheath of HD and the blade of MD, with little difference between varieties for the content of the roots. HD uses an additional mechanism to counter salinity, sequestering preferentially over sodium in the sheath unlike in the blade of MD variety. HD has a greater ability to accumulate K + ion in the limb and in the roots compared to MD. The K +/Na + ratio of leaf blade is higher in HD compared to MD. This indicates that it is mainly their leaves HD restores the balance of the osmotic potential via K+ and thus minimizes the effect of osmotic stress caused by NaCl. HD capacity to pump K + in the nutrient solution, and then to transfer to the limb, therefore a strategy for adaptation to salt stress via osmotic regulation. Results of study for the embryogenic capacity and power regeneration in vitro thermal and salt stress in MD and HD using mature embryos as explants show that MD responds better than HD in vitro selection. MD has a higher rate of induction of callus with a better callus induced
proliferation, but with fewer embryos induced by cal. The rate of regeneration of plantlets from proliferating callus was null for HD and very low for MD. The index sensitivity (IS)confirmed the tolerance of MD compared to HD for salt and thermal stress in vitro to a given parameter is the length of the roots. The results suggest the possibility of using the in vitro technique in selection for no biotic stress. Results of study for the expression analysis of five candidate genes for tolerance to salinity indicate that HKT1,5 and HKT2,2 are expressed in the roots, but not in sheaths and limbs, with better HKT1,5 gene expression in the roots of HD. This suggests a more active role HKT1, 5 genes in HD. The expression of anti-porter vacuolar Na +/H+, TNHX-1 roots, sheaths and limbs is higher in HD than in MD. Roots and sheath of the two genotypes accumulate more transcripts of the vacuolar pyrophosphatase
TVP1 than blades. Type similarity of gene expression TNHX1 and TVP1, observed in MD and HD, suggesting that vacuolar compartmentalization is equal efficiency in both genotypes.
More gene transcripts TaSOS-1, accumulate in the roots and sheath of MD compared to HD, suggesting that in addition to more effective retention of Na + in sheaths, HD avoids accumulation of Na + in the blade by activating its sodium efflux via high expression of SOS1 gene in this compartment. Results indicate that salinity tolerance in the bread wheat seems to be related to the ability to prevent the accumulation of toxic levels of Na +, associated with a high osmo-regulation and/or ability to maintain an acceptable level of K +, especially in the leaf blade. This information is useful for selection of plant material in cereal breeding programs.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BEN6415.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9435 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEN/6415 BEN/6415 Thèse Bibliothèque principale Thèses Disponible Documents numériques
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Titre : Criblage des souches d’actinobactéries pour leurs pouvoirs PGPR : Essais in vitro et in planta sur Solanum lycopersicum L., Triticum durum Desf., et Daucus carota L. Type de document : texte imprimé Auteurs : Rihab Djebaili, Auteur ; Mahmoud Kitouni, Directeur de thèse ; Maddalena Del Gallo, Directeur de thèse Mention d'édition : 11-11-2021 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2021 Importance : 244 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Microbiologie: Microbiologie générale et appliquée PGPB actinomycètes solubilisation du phosphate acide indole-3-acétique acide cyanhydrique biofertilisant ACC désaminase blé dur stress salin halotolérance la lutte biologique pathogènes SEM filtrats de culture tomate carotte actinomycetes phosphatesolubilization indole-3-acetic acid hydrocyanic acid biofertilizer ACC deaminase durum wheat salt stress halotolerance biological control pathogens culture filtrates tomato carrot Index. décimale : 570 Sciences de la vie. Biologie Résumé : Abiotic stress due to climate change and phytopathogens attacks lead to several damages in agricultural crops. Excessive use of chemicals in agriculture causes environmental pollution and loss of agricultural lands with fertile soils. Plant growth-promoting bacteria (PGPB) are an alternative strategy for sustainable agriculture. Among PGPBs, actinomycetes have recently gained increasing attention for their ability to alleviate plant stress and improve agricultural productivity. This study aimed to select actinomycetes strains with plant growth promoting traits, their plantroots association capacities, and their biostimulating effects without and with salt stress as well as the biocontrol power against several fungal and bacterial plant pathogens. The strains were studied for their phosphate solubilization capacity, indole-3-acetic acid (IAA), hydrocyanic acid (HCN),
and ammonia (NH3) production, and several enzymatic activities. Bacteria-root associations were studied by scanning electron microscopy (SEM), and a greenhouse experiment was performed to assess the inoculation effects on Solanum lycopersiumL.. Likewise, these strains were tested for in vitro halotolerant ability by several PGP tests with different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl) and by a greenhouse experiment on Triticum durum. The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also studied. Moreover, the strains were screened for their in vitro biocontrol activity by soluble and volatile compounds production against the fungal strains Fusarium oxysporum f. sp. radicis-lycopersici (FORL) and Rhizoctonia solani (RHS). The post-interaction events fungus-PGPB were analyzed by scanning electron microscopy. The antibacterial activity was evaluated against Pseudomonassyringae, Pseudomonas corrugata, Pseudomonas syringaepv. actinidiae, and Pectobacteriumcarotovorum subsp. carotovorum. Strains showing good in vitro biological control activities were studied for their antifungal and antibacterial activity using the cell culture filtrate (CFS). The consortium of potent strain was applied on Solanum lycopersicum L. and Daucus carota L. for in planta biocontrol activity. Among sixty actinomycetes, fourteen strains exhibited plant growth promoting characteristics. These strains were able to solubilize the phosphate, produce ammonia, and showed several enzymatic activities at different rates. The scanning electron microscopy revealed a good in vitro plant root association and colonization abilities. The actinomycetes inoculation influenced positively the tomato growth parameters. These strains exhibited important in vitro PGP traits under different salt concentrations. Most strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity with significant amounts of ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strainsimproved the durum wheat morpho-biochemical parameters, which recorded a significantly higher content of chlorophyll and proline than those of uninoculated controls both under normal and stressed conditions. Strains H12 (Streptomyces albidoflavus) and H14 (Nocardiopsisaegyptica) showed good in vitro antifungal activity with diffusible and volatile compounds (inhibition up to 85%) and antibacterial activity (diameter of inhibition> 10 mm). SEM micrographs showed morphological deterioration of the fungal filaments and changes in their structures. The CFS of potent strains was also active in fungal and bacterial pathogens inhibition (minimum inhibitory concentration up to 0.2%). The consortium was also able to alleviate the infection symptoms and allow a normal growth of the infected plants compared to the control. The obtained results demonstrate the efficacy of halotolerant actinomycetes strains as a biofertilizer and biocontrol agent for plant diseases management and for the alleviation of negative effects of salt stress in plants.
Note de contenu : Annexes.
Diplôme : Doctorat En ligne : ../theses/biologie/DJE7836.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11668 Criblage des souches d’actinobactéries pour leurs pouvoirs PGPR : Essais in vitro et in planta sur Solanum lycopersicum L., Triticum durum Desf., et Daucus carota L. [texte imprimé] / Rihab Djebaili, Auteur ; Mahmoud Kitouni, Directeur de thèse ; Maddalena Del Gallo, Directeur de thèse . - 11-11-2021 . - جامعة الإخوة منتوري قسنطينة, 2021 . - 244 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Microbiologie: Microbiologie générale et appliquée PGPB actinomycètes solubilisation du phosphate acide indole-3-acétique acide cyanhydrique biofertilisant ACC désaminase blé dur stress salin halotolérance la lutte biologique pathogènes SEM filtrats de culture tomate carotte actinomycetes phosphatesolubilization indole-3-acetic acid hydrocyanic acid biofertilizer ACC deaminase durum wheat salt stress halotolerance biological control pathogens culture filtrates tomato carrot Index. décimale : 570 Sciences de la vie. Biologie Résumé : Abiotic stress due to climate change and phytopathogens attacks lead to several damages in agricultural crops. Excessive use of chemicals in agriculture causes environmental pollution and loss of agricultural lands with fertile soils. Plant growth-promoting bacteria (PGPB) are an alternative strategy for sustainable agriculture. Among PGPBs, actinomycetes have recently gained increasing attention for their ability to alleviate plant stress and improve agricultural productivity. This study aimed to select actinomycetes strains with plant growth promoting traits, their plantroots association capacities, and their biostimulating effects without and with salt stress as well as the biocontrol power against several fungal and bacterial plant pathogens. The strains were studied for their phosphate solubilization capacity, indole-3-acetic acid (IAA), hydrocyanic acid (HCN),
and ammonia (NH3) production, and several enzymatic activities. Bacteria-root associations were studied by scanning electron microscopy (SEM), and a greenhouse experiment was performed to assess the inoculation effects on Solanum lycopersiumL.. Likewise, these strains were tested for in vitro halotolerant ability by several PGP tests with different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl) and by a greenhouse experiment on Triticum durum. The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also studied. Moreover, the strains were screened for their in vitro biocontrol activity by soluble and volatile compounds production against the fungal strains Fusarium oxysporum f. sp. radicis-lycopersici (FORL) and Rhizoctonia solani (RHS). The post-interaction events fungus-PGPB were analyzed by scanning electron microscopy. The antibacterial activity was evaluated against Pseudomonassyringae, Pseudomonas corrugata, Pseudomonas syringaepv. actinidiae, and Pectobacteriumcarotovorum subsp. carotovorum. Strains showing good in vitro biological control activities were studied for their antifungal and antibacterial activity using the cell culture filtrate (CFS). The consortium of potent strain was applied on Solanum lycopersicum L. and Daucus carota L. for in planta biocontrol activity. Among sixty actinomycetes, fourteen strains exhibited plant growth promoting characteristics. These strains were able to solubilize the phosphate, produce ammonia, and showed several enzymatic activities at different rates. The scanning electron microscopy revealed a good in vitro plant root association and colonization abilities. The actinomycetes inoculation influenced positively the tomato growth parameters. These strains exhibited important in vitro PGP traits under different salt concentrations. Most strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity with significant amounts of ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strainsimproved the durum wheat morpho-biochemical parameters, which recorded a significantly higher content of chlorophyll and proline than those of uninoculated controls both under normal and stressed conditions. Strains H12 (Streptomyces albidoflavus) and H14 (Nocardiopsisaegyptica) showed good in vitro antifungal activity with diffusible and volatile compounds (inhibition up to 85%) and antibacterial activity (diameter of inhibition> 10 mm). SEM micrographs showed morphological deterioration of the fungal filaments and changes in their structures. The CFS of potent strains was also active in fungal and bacterial pathogens inhibition (minimum inhibitory concentration up to 0.2%). The consortium was also able to alleviate the infection symptoms and allow a normal growth of the infected plants compared to the control. The obtained results demonstrate the efficacy of halotolerant actinomycetes strains as a biofertilizer and biocontrol agent for plant diseases management and for the alleviation of negative effects of salt stress in plants.
Note de contenu : Annexes.
Diplôme : Doctorat En ligne : ../theses/biologie/DJE7836.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11668 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité DJE/7836 DJE/7836 Thèse Bibliothèque principale Thèses Disponible Embryogénèse somatique, variations somaclonales et tolérance à la salinité chez le blé dur (Triticum durumDesf.). / Zoheira Benabdelhafid
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Titre : Embryogénèse somatique, variations somaclonales et tolérance à la salinité chez le blé dur (Triticum durumDesf.). : (Analyse génotypique et moléculaire) Type de document : texte imprimé Auteurs : Zoheira Benabdelhafid, Auteur ; Abdelhamid Djekoun, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 144 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Blé dur stress salin embryon mature embryogénèse somatique régénération in vitro variations somaclonales RAPD polymorphisme Durum wheat salt stress mature embryo somatic embryogenesis in vitro
regeneration somaclonal variation polymorphism القمح القاسي الإجهاد الملحي الجنين ناضجة مرحلة التطور الجنيني الجسدية RAPDالتشكل المرفولوجي للزراعة في الزجاج تعدد الاشكالIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The main objective of this work is to overcome the effect of salt stress on wheat
through different techniques and biotechnology approaches, which are used to improve the behavior of different varieties of durum wheat and to select the best performing. Somatic embryogenesis is currently integrated into many selection schemes since it can significantly reduce the length of improvement cycles. Development of somatic embryos was obtained after cultivation of six varieties of durum wheat (Waha, GuemGoum Rkhem, Beliouni Adnan- 2, Beni and Adnan mestina-1) on a nutrient medium supplemented with 3.5mg / l 2.4-D. The selection of tolerant varieties to the salt stress was achieved by the addition of a selective agent: NaCl. This study is based on the addition of different concentrations of NaCl (0, 4, 8,
12, 16 g/l) to the medium to study the salt stress effect on the development of somatic embryogenesis. NaCl introduced to the regeneration medium was carried out by a sequential addition of different graded concentrations to determine the callus growth rate, the water content, the content of K +, Na + contents and the K + / Na + ratio in the presence orabsence of stress. Moreover, the application of salt stress through direct addition of different NaCl concentrations during the induction phase of callogenesis was exploited to determine the influence of stress on the rate of callogenesis, the germination rate, the rate of embryogenic calli, the regeneration rate and the average number of regenerated plants per callus. in vitro regeneration has proved susceptibelty to the development of somaclonal variation, because
when callogenesis, somatic cells must be adapted to the culture medium and generate new changes in the genome. The detection of these variations is generally performed through revelation techniques by molecular methods. In this study, DNA extracted from the regenerated plants was subjected to polymerase chain reaction (PCR) that allowed, from this template DNA in the presence of arbitrary 9 RAPD primers to obtain a degree of polymorphism indicating the presence of somaclonal variation. Analysis of RAPD profiles revealed that the presence of somaclonal variation may be the result of many crop cycles, application of a severe stress weans and different responses of durum wheat genotypes for cultivation in vitro regeneration.
Diplôme : Doctorat En ligne : ../theses/biologie/BEN6928.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10280 Embryogénèse somatique, variations somaclonales et tolérance à la salinité chez le blé dur (Triticum durumDesf.). : (Analyse génotypique et moléculaire) [texte imprimé] / Zoheira Benabdelhafid, Auteur ; Abdelhamid Djekoun, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 144 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Blé dur stress salin embryon mature embryogénèse somatique régénération in vitro variations somaclonales RAPD polymorphisme Durum wheat salt stress mature embryo somatic embryogenesis in vitro
regeneration somaclonal variation polymorphism القمح القاسي الإجهاد الملحي الجنين ناضجة مرحلة التطور الجنيني الجسدية RAPDالتشكل المرفولوجي للزراعة في الزجاج تعدد الاشكالIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The main objective of this work is to overcome the effect of salt stress on wheat
through different techniques and biotechnology approaches, which are used to improve the behavior of different varieties of durum wheat and to select the best performing. Somatic embryogenesis is currently integrated into many selection schemes since it can significantly reduce the length of improvement cycles. Development of somatic embryos was obtained after cultivation of six varieties of durum wheat (Waha, GuemGoum Rkhem, Beliouni Adnan- 2, Beni and Adnan mestina-1) on a nutrient medium supplemented with 3.5mg / l 2.4-D. The selection of tolerant varieties to the salt stress was achieved by the addition of a selective agent: NaCl. This study is based on the addition of different concentrations of NaCl (0, 4, 8,
12, 16 g/l) to the medium to study the salt stress effect on the development of somatic embryogenesis. NaCl introduced to the regeneration medium was carried out by a sequential addition of different graded concentrations to determine the callus growth rate, the water content, the content of K +, Na + contents and the K + / Na + ratio in the presence orabsence of stress. Moreover, the application of salt stress through direct addition of different NaCl concentrations during the induction phase of callogenesis was exploited to determine the influence of stress on the rate of callogenesis, the germination rate, the rate of embryogenic calli, the regeneration rate and the average number of regenerated plants per callus. in vitro regeneration has proved susceptibelty to the development of somaclonal variation, because
when callogenesis, somatic cells must be adapted to the culture medium and generate new changes in the genome. The detection of these variations is generally performed through revelation techniques by molecular methods. In this study, DNA extracted from the regenerated plants was subjected to polymerase chain reaction (PCR) that allowed, from this template DNA in the presence of arbitrary 9 RAPD primers to obtain a degree of polymorphism indicating the presence of somaclonal variation. Analysis of RAPD profiles revealed that the presence of somaclonal variation may be the result of many crop cycles, application of a severe stress weans and different responses of durum wheat genotypes for cultivation in vitro regeneration.
Diplôme : Doctorat En ligne : ../theses/biologie/BEN6928.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10280 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEN/6928 BEN/6928 Thèse Bibliothèque principale Thèses Disponible Etude de l’effet de l’interaction du molybdene avec l’azote chez les fabacees cultivees en milieu salin / Salha Bouzid
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Titre : Etude de l’effet de l’interaction du molybdene avec l’azote chez les fabacees cultivees en milieu salin Type de document : texte imprimé Auteurs : Salha Bouzid, Auteur ; Chaabane Rahmoune, Directeur de thèse Année de publication : 2017 Importance : 223 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : molybdène azote stress salin Phaseolus vulgaris L. Cicer arietinum L. Lens culinaris M. molybdoenzymes RT-PCR molybdenum nitrogen salt stress المولیبدنوم النتروجین الإجھاد الملحي الفاصولیاء الحمص العدس الأنزیمات المولیبدینیة Index. décimale : 570 Sciences de la vie. Biologie Résumé : In Algeria grain legumes are considered as an important nourishing food, they are very rich in proteins. Molybdenum has an important role in several biological processes in plants, that is why our research focuses on three legumes species (bean, chickpea and lens) submitted to salt stress and in presence of molybdenum and nitrogen.
Combination of molybdenum and nitrogen in salt stress (3g/l of NaCl) condition, leads to an increase in fresh shoot biomass and chlorophyll (a+b).
Molybdenum and nitrogen stimulate synthesis of total proteins in bean but in roots of chickpea and lens, salt stress causes their increase.
In bean and chickpea shoots, xanthine dehydrogenase activity is stimulated by both molybdenum and nitrogen.
Aldehyde oxidase activity is stimulated in bean shoots by adding molybdenum and nitrogen, and in lens roots in salt stress condition.
Sulfite oxidase has a decreasing activity which decreases with time and is affected by salt stress. Nitrate reductase activity increases in time and is affected by salt stress.
The activity of the molybdenum cofactor decreases because of salt stress except for lens which increases. For bean, sulphite oxidase and xanthine dehydrogenase genes have a role in adaptation to salt stress condition.
The expression of mARC chickpea gene and mARC2 bean gene is stimulated in condition of salt stress which gives them a role to salt stress adaptation.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BOU7017.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10410 Etude de l’effet de l’interaction du molybdene avec l’azote chez les fabacees cultivees en milieu salin [texte imprimé] / Salha Bouzid, Auteur ; Chaabane Rahmoune, Directeur de thèse . - 2017 . - 223 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : molybdène azote stress salin Phaseolus vulgaris L. Cicer arietinum L. Lens culinaris M. molybdoenzymes RT-PCR molybdenum nitrogen salt stress المولیبدنوم النتروجین الإجھاد الملحي الفاصولیاء الحمص العدس الأنزیمات المولیبدینیة Index. décimale : 570 Sciences de la vie. Biologie Résumé : In Algeria grain legumes are considered as an important nourishing food, they are very rich in proteins. Molybdenum has an important role in several biological processes in plants, that is why our research focuses on three legumes species (bean, chickpea and lens) submitted to salt stress and in presence of molybdenum and nitrogen.
Combination of molybdenum and nitrogen in salt stress (3g/l of NaCl) condition, leads to an increase in fresh shoot biomass and chlorophyll (a+b).
Molybdenum and nitrogen stimulate synthesis of total proteins in bean but in roots of chickpea and lens, salt stress causes their increase.
In bean and chickpea shoots, xanthine dehydrogenase activity is stimulated by both molybdenum and nitrogen.
Aldehyde oxidase activity is stimulated in bean shoots by adding molybdenum and nitrogen, and in lens roots in salt stress condition.
Sulfite oxidase has a decreasing activity which decreases with time and is affected by salt stress. Nitrate reductase activity increases in time and is affected by salt stress.
The activity of the molybdenum cofactor decreases because of salt stress except for lens which increases. For bean, sulphite oxidase and xanthine dehydrogenase genes have a role in adaptation to salt stress condition.
The expression of mARC chickpea gene and mARC2 bean gene is stimulated in condition of salt stress which gives them a role to salt stress adaptation.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BOU7017.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10410 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/7017 BOU/7017 Thèse Bibliothèque principale Thèses Disponible