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Partager le résultat de cette recherche Interroger des sources externesContribution a l’étude des caractères morphologiques, physiologiques et des marqueurs moléculaires pour l'évaluation du polymorphisme phénotypique et génétique des espèces du genre Tamarix dans différents écotopes de la zone steppique de KHENCHELA (EST ALGERIEN) / Abdelhamid Khabtane
Titre : Contribution a l’étude des caractères morphologiques, physiologiques et des marqueurs moléculaires pour l'évaluation du polymorphisme phénotypique et génétique des espèces du genre Tamarix dans différents écotopes de la zone steppique de KHENCHELA (EST ALGERIEN) Type de document : texte imprimé Auteurs : Abdelhamid Khabtane, Auteur ; Chaabane Rahmoune, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2015 Importance : 196 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : Ecophysiologie et biotechnologie végétale Tamarix polymorphisme marqueur PCR-SSR métabolites primaires métabolites secondaires zone steppique Khenchela polymorphism PCR-SSR marquer primary and secondary metabolites steppe zone, KHENCHELA"
????? ?????? ?????? ?????? SSR-PCR ?????? ????? ??????? ? ???????? ??????? ??????? ?????Index. décimale : 570 Sciences de la vie. Biologie Résumé : In this work we have tried to put the accent on morphological, physiological and molecular markers for the evaluation of phenotypic and genetic polymorphism in the genus of Tamarix in different ecotopes of the steppe zone in KHENCHELA (Eastern Algeria) where such genus had an important interest in the rehabilitation of degraded ecosystems.
The study's sites are selected according to a north-south transect across the region where soil, orographic and climatic characteristics are very different Geological, and soil study indicate that the species of the genus of Tamarix holds in different areas, generally alkaline and saline substrates, in a semi-arid and desert climate.
The literature review has shown that the genus of Tamarix have a very important reproductive potential, with a root system very suitable for water deficit conditions with a large capacity and it is used in traditional medicine and in modern medicine in Europe.
The flora and morphometric analysis allows us to consider the floristic richness of Tamarix group and advance certain assumptions in relation to the environment.
In terms of resistance to salinity The results are significant; where the cuttings of Tamarix sp. continued to grow even at a dose of 64g / l of NaCl.
Regarding the results of statistical analysis by ANOVA, shows that there is no significance between the effect of salt dose and the accumulation of proline.
The results of the study of primary metabolites, secondary metabolites and the antimicrobial activity revealed a highly significant effect of the environment on their quantity and quality.
The study of genetic polymorphism in species of the genus of Tamarix, using molecular marker PCR-SSR type showed that the accessions studied are genetically very distant because in most cases the dissimilarity coefficients are greater than 50% which explains the large genetic polymorphism between and within species in the genus of Tamarix.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KHA6734.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9889 Contribution a l’étude des caractères morphologiques, physiologiques et des marqueurs moléculaires pour l'évaluation du polymorphisme phénotypique et génétique des espèces du genre Tamarix dans différents écotopes de la zone steppique de KHENCHELA (EST ALGERIEN) [texte imprimé] / Abdelhamid Khabtane, Auteur ; Chaabane Rahmoune, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2015 . - 196 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Ecophysiologie et biotechnologie végétale Tamarix polymorphisme marqueur PCR-SSR métabolites primaires métabolites secondaires zone steppique Khenchela polymorphism PCR-SSR marquer primary and secondary metabolites steppe zone, KHENCHELA"
????? ?????? ?????? ?????? SSR-PCR ?????? ????? ??????? ? ???????? ??????? ??????? ?????Index. décimale : 570 Sciences de la vie. Biologie Résumé : In this work we have tried to put the accent on morphological, physiological and molecular markers for the evaluation of phenotypic and genetic polymorphism in the genus of Tamarix in different ecotopes of the steppe zone in KHENCHELA (Eastern Algeria) where such genus had an important interest in the rehabilitation of degraded ecosystems.
The study's sites are selected according to a north-south transect across the region where soil, orographic and climatic characteristics are very different Geological, and soil study indicate that the species of the genus of Tamarix holds in different areas, generally alkaline and saline substrates, in a semi-arid and desert climate.
The literature review has shown that the genus of Tamarix have a very important reproductive potential, with a root system very suitable for water deficit conditions with a large capacity and it is used in traditional medicine and in modern medicine in Europe.
The flora and morphometric analysis allows us to consider the floristic richness of Tamarix group and advance certain assumptions in relation to the environment.
In terms of resistance to salinity The results are significant; where the cuttings of Tamarix sp. continued to grow even at a dose of 64g / l of NaCl.
Regarding the results of statistical analysis by ANOVA, shows that there is no significance between the effect of salt dose and the accumulation of proline.
The results of the study of primary metabolites, secondary metabolites and the antimicrobial activity revealed a highly significant effect of the environment on their quantity and quality.
The study of genetic polymorphism in species of the genus of Tamarix, using molecular marker PCR-SSR type showed that the accessions studied are genetically very distant because in most cases the dissimilarity coefficients are greater than 50% which explains the large genetic polymorphism between and within species in the genus of Tamarix.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KHA6734.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9889 Exemplaires
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Titre : Embryogénèse somatique, variations somaclonales et tolérance à la salinité chez le blé dur (Triticum durumDesf.). : (Analyse génotypique et moléculaire) Type de document : texte imprimé Auteurs : Zoheira Benabdelhafid, Auteur ; Abdelhamid Djekoun, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 144 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : Blé dur stress salin embryon mature embryogénèse somatique régénération in vitro variations somaclonales RAPD polymorphisme Durum wheat salt stress mature embryo somatic embryogenesis in vitro
regeneration somaclonal variation polymorphism ????? ?????? ??????? ?????? ?????? ????? ????? ?????? ??????? ??????? RAPD?????? ?????????? ??????? ?? ?????? ???? ???????Index. décimale : 570 Sciences de la vie. Biologie Résumé : The main objective of this work is to overcome the effect of salt stress on wheat
through different techniques and biotechnology approaches, which are used to improve the behavior of different varieties of durum wheat and to select the best performing. Somatic embryogenesis is currently integrated into many selection schemes since it can significantly reduce the length of improvement cycles. Development of somatic embryos was obtained after cultivation of six varieties of durum wheat (Waha, GuemGoum Rkhem, Beliouni Adnan- 2, Beni and Adnan mestina-1) on a nutrient medium supplemented with 3.5mg / l 2.4-D. The selection of tolerant varieties to the salt stress was achieved by the addition of a selective agent: NaCl. This study is based on the addition of different concentrations of NaCl (0, 4, 8,
12, 16 g/l) to the medium to study the salt stress effect on the development of somatic embryogenesis. NaCl introduced to the regeneration medium was carried out by a sequential addition of different graded concentrations to determine the callus growth rate, the water content, the content of K +, Na + contents and the K + / Na + ratio in the presence orabsence of stress. Moreover, the application of salt stress through direct addition of different NaCl concentrations during the induction phase of callogenesis was exploited to determine the influence of stress on the rate of callogenesis, the germination rate, the rate of embryogenic calli, the regeneration rate and the average number of regenerated plants per callus. in vitro regeneration has proved susceptibelty to the development of somaclonal variation, because
when callogenesis, somatic cells must be adapted to the culture medium and generate new changes in the genome. The detection of these variations is generally performed through revelation techniques by molecular methods. In this study, DNA extracted from the regenerated plants was subjected to polymerase chain reaction (PCR) that allowed, from this template DNA in the presence of arbitrary 9 RAPD primers to obtain a degree of polymorphism indicating the presence of somaclonal variation. Analysis of RAPD profiles revealed that the presence of somaclonal variation may be the result of many crop cycles, application of a severe stress weans and different responses of durum wheat genotypes for cultivation in vitro regeneration.
Diplôme : Doctorat En ligne : ../theses/biologie/BEN6928.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10280 Embryogénèse somatique, variations somaclonales et tolérance à la salinité chez le blé dur (Triticum durumDesf.). : (Analyse génotypique et moléculaire) [texte imprimé] / Zoheira Benabdelhafid, Auteur ; Abdelhamid Djekoun, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2016 . - 144 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Blé dur stress salin embryon mature embryogénèse somatique régénération in vitro variations somaclonales RAPD polymorphisme Durum wheat salt stress mature embryo somatic embryogenesis in vitro
regeneration somaclonal variation polymorphism ????? ?????? ??????? ?????? ?????? ????? ????? ?????? ??????? ??????? RAPD?????? ?????????? ??????? ?? ?????? ???? ???????Index. décimale : 570 Sciences de la vie. Biologie Résumé : The main objective of this work is to overcome the effect of salt stress on wheat
through different techniques and biotechnology approaches, which are used to improve the behavior of different varieties of durum wheat and to select the best performing. Somatic embryogenesis is currently integrated into many selection schemes since it can significantly reduce the length of improvement cycles. Development of somatic embryos was obtained after cultivation of six varieties of durum wheat (Waha, GuemGoum Rkhem, Beliouni Adnan- 2, Beni and Adnan mestina-1) on a nutrient medium supplemented with 3.5mg / l 2.4-D. The selection of tolerant varieties to the salt stress was achieved by the addition of a selective agent: NaCl. This study is based on the addition of different concentrations of NaCl (0, 4, 8,
12, 16 g/l) to the medium to study the salt stress effect on the development of somatic embryogenesis. NaCl introduced to the regeneration medium was carried out by a sequential addition of different graded concentrations to determine the callus growth rate, the water content, the content of K +, Na + contents and the K + / Na + ratio in the presence orabsence of stress. Moreover, the application of salt stress through direct addition of different NaCl concentrations during the induction phase of callogenesis was exploited to determine the influence of stress on the rate of callogenesis, the germination rate, the rate of embryogenic calli, the regeneration rate and the average number of regenerated plants per callus. in vitro regeneration has proved susceptibelty to the development of somaclonal variation, because
when callogenesis, somatic cells must be adapted to the culture medium and generate new changes in the genome. The detection of these variations is generally performed through revelation techniques by molecular methods. In this study, DNA extracted from the regenerated plants was subjected to polymerase chain reaction (PCR) that allowed, from this template DNA in the presence of arbitrary 9 RAPD primers to obtain a degree of polymorphism indicating the presence of somaclonal variation. Analysis of RAPD profiles revealed that the presence of somaclonal variation may be the result of many crop cycles, application of a severe stress weans and different responses of durum wheat genotypes for cultivation in vitro regeneration.
Diplôme : Doctorat En ligne : ../theses/biologie/BEN6928.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10280 Exemplaires
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Titre : Génétique mitochondriale des infertilités masculines Type de document : texte imprimé Auteurs : Mohamed Larbi Rezgoune, Auteur ; Dalila Satta, Directeur de thèse ; Abdelhamid Slama, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 187 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : mitochondrie polymorphisme infertilité masculine biologie moléculaire mitochondria polymorphism male infertility molecular biology ???????????? ???? ??????? ?????? ???????? ??????? ??? ?????? ?????????? ???????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Mitochondria play a central role in energy metabolism, homeostasis and apoptosis.
These functions are in part under the control of a mitochondrial genome, support of cytoplasmic heredity, whose transmission is only maternal. The involvement of these organelles in human reproduction is a relatively new concept that arouses scientific and medical interest. Abnormalities both qualitative and quantitative of mitochondrial DNA (mtDNA) have been associated with male fertility disorders.
Infertility concerns 10 to 15 % of couples wishing to have a child, a male component is found
in almost half of cases. In a significant proportion of these cases, a known genetic basis,
chromosomal or gene, sometimes transmitted from the parents, is involved.
In this work, in a first part, different genetic polymorphisms in humans: (CAG)n POLG1,T3801C CYP1A1 and MTHFR A1298C, and their role as potential risk factors for male infertility in an Algerian population cohort were studied by sequencing or RFLP.
Our results show that the variants described at the MTHFR and CYP1A1 genes are not associated with male infertility unlike that of POLG1 which appears to be involved in this dysfunction.
In the second part of our work, we have prospected qualitative alterations of mitochondrial
genome by analysis of macro-deletions by long PCR and quantitative by quantification of leukocyte mtDNA rate by real-time quantitative PCR (mitochondrial DNA vs nuclear) in idiopathic male infertility. The mtDNA deletion does not appear to be associated with this disorder but the assessment of the level of leukocyte mtDNA likely suggests the involvement of this parameter in male infertility especially in azoospermic and asthenospermic men.
Molecular biology has led to the discovery of unexplored etiologies until now without denigrating the clinical and biological explanatory value of many recognized etiologies.
This mitochondrial molecular appearance misunderstood opens up new perspectives and may in the future introduce profound changes in the genetic approach to male infertility.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/REZ7000.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10389 Génétique mitochondriale des infertilités masculines [texte imprimé] / Mohamed Larbi Rezgoune, Auteur ; Dalila Satta, Directeur de thèse ; Abdelhamid Slama, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2016 . - 187 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : mitochondrie polymorphisme infertilité masculine biologie moléculaire mitochondria polymorphism male infertility molecular biology ???????????? ???? ??????? ?????? ???????? ??????? ??? ?????? ?????????? ???????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Mitochondria play a central role in energy metabolism, homeostasis and apoptosis.
These functions are in part under the control of a mitochondrial genome, support of cytoplasmic heredity, whose transmission is only maternal. The involvement of these organelles in human reproduction is a relatively new concept that arouses scientific and medical interest. Abnormalities both qualitative and quantitative of mitochondrial DNA (mtDNA) have been associated with male fertility disorders.
Infertility concerns 10 to 15 % of couples wishing to have a child, a male component is found
in almost half of cases. In a significant proportion of these cases, a known genetic basis,
chromosomal or gene, sometimes transmitted from the parents, is involved.
In this work, in a first part, different genetic polymorphisms in humans: (CAG)n POLG1,T3801C CYP1A1 and MTHFR A1298C, and their role as potential risk factors for male infertility in an Algerian population cohort were studied by sequencing or RFLP.
Our results show that the variants described at the MTHFR and CYP1A1 genes are not associated with male infertility unlike that of POLG1 which appears to be involved in this dysfunction.
In the second part of our work, we have prospected qualitative alterations of mitochondrial
genome by analysis of macro-deletions by long PCR and quantitative by quantification of leukocyte mtDNA rate by real-time quantitative PCR (mitochondrial DNA vs nuclear) in idiopathic male infertility. The mtDNA deletion does not appear to be associated with this disorder but the assessment of the level of leukocyte mtDNA likely suggests the involvement of this parameter in male infertility especially in azoospermic and asthenospermic men.
Molecular biology has led to the discovery of unexplored etiologies until now without denigrating the clinical and biological explanatory value of many recognized etiologies.
This mitochondrial molecular appearance misunderstood opens up new perspectives and may in the future introduce profound changes in the genetic approach to male infertility.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/REZ7000.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10389 Exemplaires
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Titre : Caracterisation et identification de quelques cultivars d’olivier autochtones (Olea europaea l.) apport des marqueurs moleculaires Type de document : texte imprimé Auteurs : Messaoud Laib, Auteur ; Mohamed El Moncef. Bentchikou, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 134 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : Olea europaea genetic diversity IOC descriptors polymorphism molecular
markers diversité génétique descripteurs COI polymorphisme marqueurs
moléculaires ??????? ?????? ??????? ??????? ( )COI???? ??????? ????????
????????Index. décimale : 570 Sciences de la vie. Biologie Résumé : Our objective was to characterize and identify 15 olive cultivars (Olea europaea L.) grown in
the Skikda region in order to solve taxonomic confusion problems in order to better
understand the local olive-growing heritage.
Morphological characterization was performed using IOC descriptors and genetic
characterization using microsatellites and SNPs.
The results obtained showed that the characters of the endocarp and the fruit are the most
discriminating morphologically. Microsatellite markers allowed complete discrimination of
the different cultivars, whereas the selected SNPs proved ineffective.
This characterization work constitutes a database essential to any program of conservation,
enhancement and genetic improvement of our national olive-growing heritage.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/LAI7058.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10542 Caracterisation et identification de quelques cultivars d’olivier autochtones (Olea europaea l.) apport des marqueurs moleculaires [texte imprimé] / Messaoud Laib, Auteur ; Mohamed El Moncef. Bentchikou, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2017 . - 134 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Olea europaea genetic diversity IOC descriptors polymorphism molecular
markers diversité génétique descripteurs COI polymorphisme marqueurs
moléculaires ??????? ?????? ??????? ??????? ( )COI???? ??????? ????????
????????Index. décimale : 570 Sciences de la vie. Biologie Résumé : Our objective was to characterize and identify 15 olive cultivars (Olea europaea L.) grown in
the Skikda region in order to solve taxonomic confusion problems in order to better
understand the local olive-growing heritage.
Morphological characterization was performed using IOC descriptors and genetic
characterization using microsatellites and SNPs.
The results obtained showed that the characters of the endocarp and the fruit are the most
discriminating morphologically. Microsatellite markers allowed complete discrimination of
the different cultivars, whereas the selected SNPs proved ineffective.
This characterization work constitutes a database essential to any program of conservation,
enhancement and genetic improvement of our national olive-growing heritage.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/LAI7058.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10542 Exemplaires
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