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Partager le résultat de cette recherche Interroger des sources externesInfertilité liée à la qualité du génome spermatique et impact en Procréation Médicalement Assistée. / Maroua Hachemi
Titre : Infertilité liée à la qualité du génome spermatique et impact en Procréation Médicalement Assistée. Type de document : texte imprimé Auteurs : Maroua Hachemi, Auteur ; Abdlkader Rouabah, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 202 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biochimie et Biologie Cellulaire et Moléculaire: Biologie et Santé Infertilité masculine Qualité du génome spermatique Fragmentation de l’ADN spermatique Décondensation de la chromatine spermatique Male infertility Spermatic genome quality Sperm DNA fragmentation Sperm chromatin decondensation العقم عند الرجال نوعية الجينوم المنوي تجزئة الحمض النووي للحيوانات المنوية تفكيك بنية كروماتين الحيوانات المنوية Index. décimale : 570 Sciences de la vie. Biologie Résumé : Male infertility is a relatively common pathology; the genetic etiology may be a cause of this abnormality for these males presumed sterile. Thanks to new techniques of Medically Assisted Procreation that infertile men could become biological fathers. However, some questions about the genetic and epigenetic information transmitted to the offspring as well as the modalities of this transmission are raised. A genetic study was carried out over 101 infertile male patients candidates for the Medically Assisted Procreation, these subjects are registered with their spouses in a schedule of intra-cytoplasmic microinjection of the spermatozoon. This study was performed in a prospective setting using cytogenetic tools. Two approaches have been tried in order to assess the quality of the sperm genome. One by analyzing the quality of sperm DNA by measuring the rate of degradation and fragmentation of the latter, another by exploring the quality of spermatic chromatin by measuring its decondensation rate. The study of the association between the quality of the spermatic genome and the various classical spermiological factors shows that the alteration of spermatogenesis is related to a deterioration of the spermatic genome. Regarding the behavioral, toxic and environmental factors, our study showed that these factors did not act on the quality of the spermatic genome. Nevertheless, smoking intoxication seems to alter the chromatin structure of a specific category of patients (p = 0.03). The evaluation of the various parameters was related to the status of Medically Assisted Reproduction especially those of microinjection into cytoplasmic sperm. Our results showed that alterations in the quality of the spermatic genome do not influence either the fertilization process or preimplantation embryonic development (p > 0.05). Our findings indicate also that the quality of the spermatic genome is not related to pregnancy. Nonetheless, it may have an impact on abortions. Regarding births, the integrity of the spermatic nucleus may influence the birth rate (p = 0.064). Moreover, a differentiated sperm cell with an unaltered DNA can give birth even if its chromatin is poorly structured or even decondensed.
Note de contenu : Annexes.
Diplôme : Doctorat En ligne : ../theses/biologie/HAC7520.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11359 Infertilité liée à la qualité du génome spermatique et impact en Procréation Médicalement Assistée. [texte imprimé] / Maroua Hachemi, Auteur ; Abdlkader Rouabah, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2019 . - 202 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biochimie et Biologie Cellulaire et Moléculaire: Biologie et Santé Infertilité masculine Qualité du génome spermatique Fragmentation de l’ADN spermatique Décondensation de la chromatine spermatique Male infertility Spermatic genome quality Sperm DNA fragmentation Sperm chromatin decondensation العقم عند الرجال نوعية الجينوم المنوي تجزئة الحمض النووي للحيوانات المنوية تفكيك بنية كروماتين الحيوانات المنوية Index. décimale : 570 Sciences de la vie. Biologie Résumé : Male infertility is a relatively common pathology; the genetic etiology may be a cause of this abnormality for these males presumed sterile. Thanks to new techniques of Medically Assisted Procreation that infertile men could become biological fathers. However, some questions about the genetic and epigenetic information transmitted to the offspring as well as the modalities of this transmission are raised. A genetic study was carried out over 101 infertile male patients candidates for the Medically Assisted Procreation, these subjects are registered with their spouses in a schedule of intra-cytoplasmic microinjection of the spermatozoon. This study was performed in a prospective setting using cytogenetic tools. Two approaches have been tried in order to assess the quality of the sperm genome. One by analyzing the quality of sperm DNA by measuring the rate of degradation and fragmentation of the latter, another by exploring the quality of spermatic chromatin by measuring its decondensation rate. The study of the association between the quality of the spermatic genome and the various classical spermiological factors shows that the alteration of spermatogenesis is related to a deterioration of the spermatic genome. Regarding the behavioral, toxic and environmental factors, our study showed that these factors did not act on the quality of the spermatic genome. Nevertheless, smoking intoxication seems to alter the chromatin structure of a specific category of patients (p = 0.03). The evaluation of the various parameters was related to the status of Medically Assisted Reproduction especially those of microinjection into cytoplasmic sperm. Our results showed that alterations in the quality of the spermatic genome do not influence either the fertilization process or preimplantation embryonic development (p > 0.05). Our findings indicate also that the quality of the spermatic genome is not related to pregnancy. Nonetheless, it may have an impact on abortions. Regarding births, the integrity of the spermatic nucleus may influence the birth rate (p = 0.064). Moreover, a differentiated sperm cell with an unaltered DNA can give birth even if its chromatin is poorly structured or even decondensed.
Note de contenu : Annexes.
Diplôme : Doctorat En ligne : ../theses/biologie/HAC7520.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11359 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité HAC/7520 HAC/7520 Thèse Bibliothèque principale Thèses Disponible
Titre : Les anomalies morphologiques responsables des infertilités masculines dans l’Est Algérien : Aspect épidémiologique et génétique Type de document : texte imprimé Auteurs : Leyla Ounis, Auteur ; L Rouabah, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 171 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Infertilité masculine infertilité primaire infertilité secondaire spermogramme spermocytogramme AURKC macrocéphalie DPY19L2 Male infertility primary infertility secondary infertility semen analysis spermocytogram macrocephaly العقم عند الرجال العقم الابتدائي العقم الثانوي Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Male infertility is a relatively common condition in our country. Multiple male etiological factors are what guides us to an epidemiological study of the various factors behavioral and environmental risk decreasing sperm parameters. the second part of this study aims is to achieve the molecular exploration of rare forms of male infertility related to monomorphic tératozoospermia (macrozoospermia and globozoospermia), determining the newly described mutations in genes AURORA KINASE C and DPY19L2 respectively and determining for the first time the frequency of these unusual anomalies in a population of Algerian origin.
This is a retrospective descriptive study of 404 patients intended by their doctor for infertility diagnosis and intended for an AMP. Epidemiological, clinical and laboratory parameters were recorded for each patient. 19 patients with OAT with rare severe morphological abnormalities (globozoospermia, macrozoospermia) were selected for DNA analysis. 7 exons of the AURKC gene were amplified looking for a mutation in one of
these exons and the MLPA technique was performed in search of a deletion of DPY19L2.
The frequency of patients with semen analysis and / or medical spermocytogramme was 83.3% with a predominance of sperm abnormality oligo-astheno-teratospermia (OAT).
This study shows a moderate effect of environmental factors and behavioral risk on sperm parameters. The exposure to heat and toxins appear to be the most detrimental factor for spermatogenesis. There was a significant decrease in the count exposed to high heat and /or toxic inhalations. Fourteen men and seven respectively showed a typical profile of macrozoospermia and globozoospermia. Molecular diagnosis of AURKC and DPY19L2 was performed for these patients. Eleven men with macrozoospermia had a homozygous
mutation AURKC (79%) indicating that mutations AURKC were identified in 2.7% of patients with abnormal semen analysis. All men with globozoospermia analyzed (n = 5) showed a homozygous deletion DPY19L2 or 1.2% of infertile men. This study confirmed the recurrence of two mutations AURKC and DPY19L2 deletion in Algerian infertile men.
We observed that macrozoospermia is 2-3 times more common than globozoospermia. Our results confirm that ICSI can not be effective for patients with AURKC homozygous mutation. This highlights the importance of molecular diagnostics of AURKC gene to limit unnecessary ICSI attempts.Diplôme : Doctorat En ligne : ../theses/biologie/OUN6639.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9773 Les anomalies morphologiques responsables des infertilités masculines dans l’Est Algérien : Aspect épidémiologique et génétique [texte imprimé] / Leyla Ounis, Auteur ; L Rouabah, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 171 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Infertilité masculine infertilité primaire infertilité secondaire spermogramme spermocytogramme AURKC macrocéphalie DPY19L2 Male infertility primary infertility secondary infertility semen analysis spermocytogram macrocephaly العقم عند الرجال العقم الابتدائي العقم الثانوي Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Male infertility is a relatively common condition in our country. Multiple male etiological factors are what guides us to an epidemiological study of the various factors behavioral and environmental risk decreasing sperm parameters. the second part of this study aims is to achieve the molecular exploration of rare forms of male infertility related to monomorphic tératozoospermia (macrozoospermia and globozoospermia), determining the newly described mutations in genes AURORA KINASE C and DPY19L2 respectively and determining for the first time the frequency of these unusual anomalies in a population of Algerian origin.
This is a retrospective descriptive study of 404 patients intended by their doctor for infertility diagnosis and intended for an AMP. Epidemiological, clinical and laboratory parameters were recorded for each patient. 19 patients with OAT with rare severe morphological abnormalities (globozoospermia, macrozoospermia) were selected for DNA analysis. 7 exons of the AURKC gene were amplified looking for a mutation in one of
these exons and the MLPA technique was performed in search of a deletion of DPY19L2.
The frequency of patients with semen analysis and / or medical spermocytogramme was 83.3% with a predominance of sperm abnormality oligo-astheno-teratospermia (OAT).
This study shows a moderate effect of environmental factors and behavioral risk on sperm parameters. The exposure to heat and toxins appear to be the most detrimental factor for spermatogenesis. There was a significant decrease in the count exposed to high heat and /or toxic inhalations. Fourteen men and seven respectively showed a typical profile of macrozoospermia and globozoospermia. Molecular diagnosis of AURKC and DPY19L2 was performed for these patients. Eleven men with macrozoospermia had a homozygous
mutation AURKC (79%) indicating that mutations AURKC were identified in 2.7% of patients with abnormal semen analysis. All men with globozoospermia analyzed (n = 5) showed a homozygous deletion DPY19L2 or 1.2% of infertile men. This study confirmed the recurrence of two mutations AURKC and DPY19L2 deletion in Algerian infertile men.
We observed that macrozoospermia is 2-3 times more common than globozoospermia. Our results confirm that ICSI can not be effective for patients with AURKC homozygous mutation. This highlights the importance of molecular diagnostics of AURKC gene to limit unnecessary ICSI attempts.Diplôme : Doctorat En ligne : ../theses/biologie/OUN6639.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9773 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité OUN/6639 OUN/6639 Thèse Bibliothèque principale Thèses Disponible
Titre : Génétique mitochondriale des infertilités masculines Type de document : texte imprimé Auteurs : Mohamed Larbi Rezgoune, Auteur ; Dalila Satta, Directeur de thèse ; Abdelhamid Slama, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 187 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : mitochondrie polymorphisme infertilité masculine biologie moléculaire mitochondria polymorphism male infertility molecular biology الميتوكوندري تعدد الاشكال الجيني اصطرابات الخصوبة عند الرجال البيولوجيا الجزيئية Index. décimale : 570 Sciences de la vie. Biologie Résumé : Mitochondria play a central role in energy metabolism, homeostasis and apoptosis.
These functions are in part under the control of a mitochondrial genome, support of cytoplasmic heredity, whose transmission is only maternal. The involvement of these organelles in human reproduction is a relatively new concept that arouses scientific and medical interest. Abnormalities both qualitative and quantitative of mitochondrial DNA (mtDNA) have been associated with male fertility disorders.
Infertility concerns 10 to 15 % of couples wishing to have a child, a male component is found
in almost half of cases. In a significant proportion of these cases, a known genetic basis,
chromosomal or gene, sometimes transmitted from the parents, is involved.
In this work, in a first part, different genetic polymorphisms in humans: (CAG)n POLG1,T3801C CYP1A1 and MTHFR A1298C, and their role as potential risk factors for male infertility in an Algerian population cohort were studied by sequencing or RFLP.
Our results show that the variants described at the MTHFR and CYP1A1 genes are not associated with male infertility unlike that of POLG1 which appears to be involved in this dysfunction.
In the second part of our work, we have prospected qualitative alterations of mitochondrial
genome by analysis of macro-deletions by long PCR and quantitative by quantification of leukocyte mtDNA rate by real-time quantitative PCR (mitochondrial DNA vs nuclear) in idiopathic male infertility. The mtDNA deletion does not appear to be associated with this disorder but the assessment of the level of leukocyte mtDNA likely suggests the involvement of this parameter in male infertility especially in azoospermic and asthenospermic men.
Molecular biology has led to the discovery of unexplored etiologies until now without denigrating the clinical and biological explanatory value of many recognized etiologies.
This mitochondrial molecular appearance misunderstood opens up new perspectives and may in the future introduce profound changes in the genetic approach to male infertility.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/REZ7000.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10389 Génétique mitochondriale des infertilités masculines [texte imprimé] / Mohamed Larbi Rezgoune, Auteur ; Dalila Satta, Directeur de thèse ; Abdelhamid Slama, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 187 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : mitochondrie polymorphisme infertilité masculine biologie moléculaire mitochondria polymorphism male infertility molecular biology الميتوكوندري تعدد الاشكال الجيني اصطرابات الخصوبة عند الرجال البيولوجيا الجزيئية Index. décimale : 570 Sciences de la vie. Biologie Résumé : Mitochondria play a central role in energy metabolism, homeostasis and apoptosis.
These functions are in part under the control of a mitochondrial genome, support of cytoplasmic heredity, whose transmission is only maternal. The involvement of these organelles in human reproduction is a relatively new concept that arouses scientific and medical interest. Abnormalities both qualitative and quantitative of mitochondrial DNA (mtDNA) have been associated with male fertility disorders.
Infertility concerns 10 to 15 % of couples wishing to have a child, a male component is found
in almost half of cases. In a significant proportion of these cases, a known genetic basis,
chromosomal or gene, sometimes transmitted from the parents, is involved.
In this work, in a first part, different genetic polymorphisms in humans: (CAG)n POLG1,T3801C CYP1A1 and MTHFR A1298C, and their role as potential risk factors for male infertility in an Algerian population cohort were studied by sequencing or RFLP.
Our results show that the variants described at the MTHFR and CYP1A1 genes are not associated with male infertility unlike that of POLG1 which appears to be involved in this dysfunction.
In the second part of our work, we have prospected qualitative alterations of mitochondrial
genome by analysis of macro-deletions by long PCR and quantitative by quantification of leukocyte mtDNA rate by real-time quantitative PCR (mitochondrial DNA vs nuclear) in idiopathic male infertility. The mtDNA deletion does not appear to be associated with this disorder but the assessment of the level of leukocyte mtDNA likely suggests the involvement of this parameter in male infertility especially in azoospermic and asthenospermic men.
Molecular biology has led to the discovery of unexplored etiologies until now without denigrating the clinical and biological explanatory value of many recognized etiologies.
This mitochondrial molecular appearance misunderstood opens up new perspectives and may in the future introduce profound changes in the genetic approach to male infertility.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/REZ7000.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10389 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité REZ/7000 REZ/7000 Thèse Bibliothèque principale Thèses Disponible
