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Partager le résultat de cette recherche Interroger des sources externesEvaluation, in vitro, du potentiel du chitosane pour la lutte contre les champignons responsables des Onychomycoses à Constantine / Anissa Zohra Hafirassou
Titre : Evaluation, in vitro, du potentiel du chitosane pour la lutte contre les champignons responsables des Onychomycoses à Constantine Type de document : texte imprimé Auteurs : Anissa Zohra Hafirassou, Auteur ; I Mihoubi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 127 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : Onychomycoses Chitosane PCR Dermatophytes Activité antifongique Onychomycosis Chitosan Antifungal Activity ??????????? ????????? ???????? ??????? ?????? ?????? Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Onychomycosis are frequent, contagious, benign and chronicnail fungal infections. Dermatophytes are considered as the most implicated pathogens but molds and yeasts can also be colonizersor contaminantsagents. Onychomycosis are often observed at an advanced age and an evolved stage, making their treatment long and difficult because of the lack of less or non-toxic molecules used at therapeutic doses.
In this context, the main objective of the study was to produce, from Rhizopusoryzae strain, an FDA-approved biopolymer: the chitosan. And evaluate its antifungal activity on the species responsible for onychomycosis in Constantine.
298 samples of infected nails were collected at the laboratory of Mycology and Parasitology of CHUC and analyzed using conventional diagnosis (direct observation and culture) and molecular RT-PCR methods (panfungique-PCR, pandermatophyte- PCR).
The results obtained showed that T.rubrum was the predominant species. However, a varietyTrichophytonrubrum var. raubitschekii was identified for the first time in Algeria. As for the synthesized chitosan, its characterization by FTIR and titration revealed a good quality with a slightly higher degree of deacetylation than that of commercial chitosan. Furthermore, demonstration of antifungal activity on the most incriminated species in onychomycoses
revealed a significant effect with MICs of 2.5, 1.25 and 0.62mg / ml on T. rubrum, T.rubrum var. raubitschekii and T.interdigitale,respectively. These results could open the way to new therapeutic perspectives, not only against dermatophytes but probably against other fungal agents.
Diplôme : Doctorat En ligne : ../theses/biologie/HAF7018.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10418 Evaluation, in vitro, du potentiel du chitosane pour la lutte contre les champignons responsables des Onychomycoses à Constantine [texte imprimé] / Anissa Zohra Hafirassou, Auteur ; I Mihoubi, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2017 . - 127 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Onychomycoses Chitosane PCR Dermatophytes Activité antifongique Onychomycosis Chitosan Antifungal Activity ??????????? ????????? ???????? ??????? ?????? ?????? Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Onychomycosis are frequent, contagious, benign and chronicnail fungal infections. Dermatophytes are considered as the most implicated pathogens but molds and yeasts can also be colonizersor contaminantsagents. Onychomycosis are often observed at an advanced age and an evolved stage, making their treatment long and difficult because of the lack of less or non-toxic molecules used at therapeutic doses.
In this context, the main objective of the study was to produce, from Rhizopusoryzae strain, an FDA-approved biopolymer: the chitosan. And evaluate its antifungal activity on the species responsible for onychomycosis in Constantine.
298 samples of infected nails were collected at the laboratory of Mycology and Parasitology of CHUC and analyzed using conventional diagnosis (direct observation and culture) and molecular RT-PCR methods (panfungique-PCR, pandermatophyte- PCR).
The results obtained showed that T.rubrum was the predominant species. However, a varietyTrichophytonrubrum var. raubitschekii was identified for the first time in Algeria. As for the synthesized chitosan, its characterization by FTIR and titration revealed a good quality with a slightly higher degree of deacetylation than that of commercial chitosan. Furthermore, demonstration of antifungal activity on the most incriminated species in onychomycoses
revealed a significant effect with MICs of 2.5, 1.25 and 0.62mg / ml on T. rubrum, T.rubrum var. raubitschekii and T.interdigitale,respectively. These results could open the way to new therapeutic perspectives, not only against dermatophytes but probably against other fungal agents.
Diplôme : Doctorat En ligne : ../theses/biologie/HAF7018.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10418 Exemplaires
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Titre : Etude des Leishmanioses diagnostiquées au centre hospitalo-universitaire Ben Badis de Constantine Type de document : texte imprimé Auteurs : Université Mentouri, Editeur scientifique Année de publication : 2006 Importance : 127 f. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Tags : Diagnostic Constantine PCR Leishmaniose CUTAN2E . Leishmaniose viscérale Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/DJE4726.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1349 Etude des Leishmanioses diagnostiquées au centre hospitalo-universitaire Ben Badis de Constantine [texte imprimé] / Université Mentouri, Editeur scientifique . - 2006 . - 127 f.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Tags : Diagnostic Constantine PCR Leishmaniose CUTAN2E . Leishmaniose viscérale Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/DJE4726.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1349 Exemplaires
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Titre : Recherche de la mutation DELTA F508 du gène CFTR dans la population de Constantine Type de document : texte imprimé Auteurs : Khadidja Sedrati, Auteur ; D. Naimi, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2008 Importance : 81 f. Format : 31 cm Note générale : Magister
2 copies imprimées disponiblesLangues : Français (fre) Tags : Algérie Mutation Mucoviscidose GLne CFTR . Proteine cftr PCR Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/SED5097.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1377 Recherche de la mutation DELTA F508 du gène CFTR dans la population de Constantine [texte imprimé] / Khadidja Sedrati, Auteur ; D. Naimi, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2008 . - 81 f. ; 31 cm.
Magister
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Algérie Mutation Mucoviscidose GLne CFTR . Proteine cftr PCR Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/SED5097.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1377 Exemplaires
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Titre : Diagnostic moléculaire des amyotrophies spinales : recherche de la délétion de l'exon 8 du gène SMN Type de document : texte imprimé Auteurs : Université Mentouri, Editeur scientifique Année de publication : 2008 Importance : 116 f. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Tags : PCR SMA GLne SMA Exon 8 Exon 7 Délétion Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/HAM5158.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1799 Diagnostic moléculaire des amyotrophies spinales : recherche de la délétion de l'exon 8 du gène SMN [texte imprimé] / Université Mentouri, Editeur scientifique . - 2008 . - 116 f.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Tags : PCR SMA GLne SMA Exon 8 Exon 7 Délétion Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/HAM5158.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1799 Exemplaires
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Titre : Contribution a l'étude des procèdes électrochimiques d'adoucissement des eaux dures Type de document : texte imprimé Auteurs : Yasmina Boulahèib (Née Bendaoud), Auteur ; Samira Ghizellaoui, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2015 Importance : 187 f. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : Entartrage traitement chimique PCR chronoampérométrie impédancemétrie MEB KH2PO4 Polyphosphates HEDP Scaling chemical treatment chronoamperometry impedance SEM ???? ?????? ???????? ?????? ??????? ?????? ?????????????????? ?????? ????????? ???????? ????? Index. décimale : 540 Chimie et sciences connexes Résumé : Treating hard water has been interest from several works in order to reduce the adverse consequences of tartar. Hard water deposits form compact and adherent to the walls of pipes and industrial or domestic installations (GHIZELLAOUI, 2006).
Our study focuses on softening hard water Hamma having a hardness of 59°F and Negrine with a hardness of 56°F in the presence of KH2PO4, polyphosphates and HEDP.
The evaluation and the inhibition of scaling of hard waters of Hamma and Negrine were carried out by using four methods:
- The chemical method for evaluating the effective concentration of inhibitor to reduce the hardness of waters of Hamma and Negrine at temperature sources 30°C while maintaining a residual calcium concentration of 1 00 mg/L.
- The method of rapid precipitation controlled to track the phenomenon of germination growth during the formation of the deposit of calcium carbonate.
- The electrochemical method of accelerated scaling to assess the scaling power of raw waters of Hamma and Negrine by electrochemical way and in the presence of chemical inhibitors (KH2PO4, polyphosphates and HEDP );
- electrochemical method for measuring impedances of precipitated deposits metal deposit calcium carbonate
- electrolyte interface enables an overall assessment by means of the highfrequency resistance , adhesion and the importance of filing carbonate calcium ( Khalil et al.1993).
-The effect of inhibitors on the morphology and adhesion of the deposit of calcium carbonate was observed by the scanning electron microscope SEM and RX.
The application of chemical treatment of the waters of Hamma and Negrine at temperature of 30°C showed that partial inhibition of hardness requires addition of 2 mg/L of KH2PO4 for Hamma and 3 mg/L for Negrine. By against, the use of the polyphosphates inhibits scaling at a concentration of 10 mg/L for Hamma water and 5 mg/L for Negrine water.
The HEDP effect is at a concentration of 1 00 mg/L for Hamma and 50 mg/L for Negrine.
These values are reduced using the method of rapid controlled precipitation for each of the three inhibitors (0.1 mg/L KH2PO4, 0.01 mg/L Polyphosphates or 0.01 mg/L HEDP) for water of Hamma, and (0.5 mg/L KH2PO4, 1 mg/L Polyphosphates or 0.01 mg/L HEDP ) for water of Negrine.
The chronoamperometric study shows that the scaling time increases with the addition of (0.1 mg/L KH2PO4, 0.04 mg/L Polyphosphates or 0.005 mg/L HEDP ) for Hamma water and (0.5 mg/L KH2PO4, 0.25 mg/L Polyphosphtes or 10-5 mg/L HEDP) for Negrine water.
The impedance measurement obtained from the raw waters of Hamma and Negrine compared to treated waters shows a decrease in the amount of calcium carbonate scale as well as its adhesion by measuring high frequency resistors deposits from addition of (0.01 mg/L KH2PO4, 0.01 mg/L Polyphosphates or 0.005 mg/L HEDP) for Hamma also (0.5 mg/L KH2PO4, 0.05 mg/L Polyphosphates or 0.0075 mg/L HEDP) for Negrine.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/BOU6778.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9831 Contribution a l'étude des procèdes électrochimiques d'adoucissement des eaux dures [texte imprimé] / Yasmina Boulahèib (Née Bendaoud), Auteur ; Samira Ghizellaoui, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2015 . - 187 f.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Entartrage traitement chimique PCR chronoampérométrie impédancemétrie MEB KH2PO4 Polyphosphates HEDP Scaling chemical treatment chronoamperometry impedance SEM ???? ?????? ???????? ?????? ??????? ?????? ?????????????????? ?????? ????????? ???????? ????? Index. décimale : 540 Chimie et sciences connexes Résumé : Treating hard water has been interest from several works in order to reduce the adverse consequences of tartar. Hard water deposits form compact and adherent to the walls of pipes and industrial or domestic installations (GHIZELLAOUI, 2006).
Our study focuses on softening hard water Hamma having a hardness of 59°F and Negrine with a hardness of 56°F in the presence of KH2PO4, polyphosphates and HEDP.
The evaluation and the inhibition of scaling of hard waters of Hamma and Negrine were carried out by using four methods:
- The chemical method for evaluating the effective concentration of inhibitor to reduce the hardness of waters of Hamma and Negrine at temperature sources 30°C while maintaining a residual calcium concentration of 1 00 mg/L.
- The method of rapid precipitation controlled to track the phenomenon of germination growth during the formation of the deposit of calcium carbonate.
- The electrochemical method of accelerated scaling to assess the scaling power of raw waters of Hamma and Negrine by electrochemical way and in the presence of chemical inhibitors (KH2PO4, polyphosphates and HEDP );
- electrochemical method for measuring impedances of precipitated deposits metal deposit calcium carbonate
- electrolyte interface enables an overall assessment by means of the highfrequency resistance , adhesion and the importance of filing carbonate calcium ( Khalil et al.1993).
-The effect of inhibitors on the morphology and adhesion of the deposit of calcium carbonate was observed by the scanning electron microscope SEM and RX.
The application of chemical treatment of the waters of Hamma and Negrine at temperature of 30°C showed that partial inhibition of hardness requires addition of 2 mg/L of KH2PO4 for Hamma and 3 mg/L for Negrine. By against, the use of the polyphosphates inhibits scaling at a concentration of 10 mg/L for Hamma water and 5 mg/L for Negrine water.
The HEDP effect is at a concentration of 1 00 mg/L for Hamma and 50 mg/L for Negrine.
These values are reduced using the method of rapid controlled precipitation for each of the three inhibitors (0.1 mg/L KH2PO4, 0.01 mg/L Polyphosphates or 0.01 mg/L HEDP) for water of Hamma, and (0.5 mg/L KH2PO4, 1 mg/L Polyphosphates or 0.01 mg/L HEDP ) for water of Negrine.
The chronoamperometric study shows that the scaling time increases with the addition of (0.1 mg/L KH2PO4, 0.04 mg/L Polyphosphates or 0.005 mg/L HEDP ) for Hamma water and (0.5 mg/L KH2PO4, 0.25 mg/L Polyphosphtes or 10-5 mg/L HEDP) for Negrine water.
The impedance measurement obtained from the raw waters of Hamma and Negrine compared to treated waters shows a decrease in the amount of calcium carbonate scale as well as its adhesion by measuring high frequency resistors deposits from addition of (0.01 mg/L KH2PO4, 0.01 mg/L Polyphosphates or 0.005 mg/L HEDP) for Hamma also (0.5 mg/L KH2PO4, 0.05 mg/L Polyphosphates or 0.0075 mg/L HEDP) for Negrine.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/BOU6778.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9831 Exemplaires
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