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Partager le résultat de cette recherche Interroger des sources externesEvaluation, in vitro, du potentiel du chitosane pour la lutte contre les champignons responsables des Onychomycoses à Constantine / Anissa Zohra Hafirassou
Titre : Evaluation, in vitro, du potentiel du chitosane pour la lutte contre les champignons responsables des Onychomycoses à Constantine Type de document : texte imprimé Auteurs : Anissa Zohra Hafirassou, Auteur ; I Mihoubi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 127 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Onychomycoses Chitosane PCR Dermatophytes Activité antifongique Onychomycosis Chitosan Antifungal Activity ??????????? ????????? ???????? ??????? ?????? ?????? Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Onychomycosis are frequent, contagious, benign and chronicnail fungal infections. Dermatophytes are considered as the most implicated pathogens but molds and yeasts can also be colonizersor contaminantsagents. Onychomycosis are often observed at an advanced age and an evolved stage, making their treatment long and difficult because of the lack of less or non-toxic molecules used at therapeutic doses.
In this context, the main objective of the study was to produce, from Rhizopusoryzae strain, an FDA-approved biopolymer: the chitosan. And evaluate its antifungal activity on the species responsible for onychomycosis in Constantine.
298 samples of infected nails were collected at the laboratory of Mycology and Parasitology of CHUC and analyzed using conventional diagnosis (direct observation and culture) and molecular RT-PCR methods (panfungique-PCR, pandermatophyte- PCR).
The results obtained showed that T.rubrum was the predominant species. However, a varietyTrichophytonrubrum var. raubitschekii was identified for the first time in Algeria. As for the synthesized chitosan, its characterization by FTIR and titration revealed a good quality with a slightly higher degree of deacetylation than that of commercial chitosan. Furthermore, demonstration of antifungal activity on the most incriminated species in onychomycoses
revealed a significant effect with MICs of 2.5, 1.25 and 0.62mg / ml on T. rubrum, T.rubrum var. raubitschekii and T.interdigitale,respectively. These results could open the way to new therapeutic perspectives, not only against dermatophytes but probably against other fungal agents.
Diplôme : Doctorat En ligne : ../theses/biologie/HAF7018.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10418 Evaluation, in vitro, du potentiel du chitosane pour la lutte contre les champignons responsables des Onychomycoses à Constantine [texte imprimé] / Anissa Zohra Hafirassou, Auteur ; I Mihoubi, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2017 . - 127 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Onychomycoses Chitosane PCR Dermatophytes Activité antifongique Onychomycosis Chitosan Antifungal Activity ??????????? ????????? ???????? ??????? ?????? ?????? Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Onychomycosis are frequent, contagious, benign and chronicnail fungal infections. Dermatophytes are considered as the most implicated pathogens but molds and yeasts can also be colonizersor contaminantsagents. Onychomycosis are often observed at an advanced age and an evolved stage, making their treatment long and difficult because of the lack of less or non-toxic molecules used at therapeutic doses.
In this context, the main objective of the study was to produce, from Rhizopusoryzae strain, an FDA-approved biopolymer: the chitosan. And evaluate its antifungal activity on the species responsible for onychomycosis in Constantine.
298 samples of infected nails were collected at the laboratory of Mycology and Parasitology of CHUC and analyzed using conventional diagnosis (direct observation and culture) and molecular RT-PCR methods (panfungique-PCR, pandermatophyte- PCR).
The results obtained showed that T.rubrum was the predominant species. However, a varietyTrichophytonrubrum var. raubitschekii was identified for the first time in Algeria. As for the synthesized chitosan, its characterization by FTIR and titration revealed a good quality with a slightly higher degree of deacetylation than that of commercial chitosan. Furthermore, demonstration of antifungal activity on the most incriminated species in onychomycoses
revealed a significant effect with MICs of 2.5, 1.25 and 0.62mg / ml on T. rubrum, T.rubrum var. raubitschekii and T.interdigitale,respectively. These results could open the way to new therapeutic perspectives, not only against dermatophytes but probably against other fungal agents.
Diplôme : Doctorat En ligne : ../theses/biologie/HAF7018.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10418 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité HAF/7018 HAF/7018 Thèse Bibliothèque principale Thèses Disponible
Titre : Contribution au diagnostic de la theilériose chez les bovins dans l’Est algérien Type de document : texte imprimé Auteurs : Ouarda Ayadi, Auteur ; Mohamed Cherif Benchikh Elfgoun, Auteur Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 152 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
Sciences VétérinairesTags : Theilériose tropicale Bovins PCR étalement sanguin biochimie hématologie lait Algérie Tropical theileriosis cattle blood smear biochemistry haematology milk Algeria ????? ???????? ?????????? ??????? ??????? ??????????? ???????? ?????? ?????? ????????
??????? ???????? ??????? ??????Index. décimale : 636 Sciences Vétérinaires Résumé :
The present study carried out in the region of El Eulma (North-eastern Algeria) showed a highest prevalence (81.9%) of theileriosis (Theileria. annulata infection) among the piroplasmosis, the coinfection between theileriosis and babesiosis (B. bovis) are frequent (14.9%). The number of the highest cases (45) has parasitemia between 0.1 - 4.99%. A total of 180 and 134 blood samples from 21 farms were collected in April and November 2015 (before and after the tropical theileriosis season; respectively) in the region of El Eulma (Sétif). Molecular prevalence (38.9 and 50% in April and November; respectively) was higher than those by Giemsa stained blood smear (17 and 16.4%, before and after tropical theileriosis season; respectively) (p<0.001 ). Exotic breeds were more infected than local breeds in the first visit (p=0.003), while during the second visit no significant difference was detected (p=0.226). This prevalence is lower in cattle less than one year (p=0,117; 0,270 in April and November; respectively). Molecular prevalence was significantly higher in females (44.7%) than in males (22.9%) (p<0.008) during the first visit.
No influence of type of livestock or the walls statements was detected. The sequences of genes Algerian Tams1 (96.1 to 98.9%, identity values) have a relationship with the Mauritanian, Tunisian and Egyptian sequences.
The biochemical and haematological study conducted on 40 healthy cattle and 40 clinically infected showed that the number of leukocytes, erythrocytes, haemoglobin, haematocrit, glucose, albumin, and total protein decrease significantly (p=0.005; 0.008, 0.03,0.048, 0.002, 0.027 and 0.018; respectively) in clinically infected Montbeliard breed with Theileria annulata. All infected animals have a total and direct bilirubin concentration increase significantly (p<0.001). The erythrocytes, haemoglobin, and haematocrit crossbreed cattle concentration decrease significantly (p=0.027; 0.003 and <0.001; respectively). Two types of anaemia were detected: hypochromic microcytic anaemia in Montbeliard clinically infected cattle and normochromic normocytic anaemia in infected local and cross breed cattle.
The tropical theileriosis impact study in Skikda region (northern Algeria) showed a decrease in milk production in 10 local breed cattle infected with Theileria annulata in summer 2015. The decrease in weekly milk production estimated for two months was 2.76 L /day / cow corresponding to 31.92% of the total milk production. This decrease corresponds to 110.5 AD (US $ 1.02) / day / sick cow. The relative variation of milk production showed a dramatic decrease from 82.72 to 0.76% on day 21.
Diplôme : Doctorat en sciences En ligne : ../theses/veterinaire/AYA6981.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10414 Contribution au diagnostic de la theilériose chez les bovins dans l’Est algérien [texte imprimé] / Ouarda Ayadi, Auteur ; Mohamed Cherif Benchikh Elfgoun, Auteur . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2016 . - 152 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
Sciences VétérinairesTags : Theilériose tropicale Bovins PCR étalement sanguin biochimie hématologie lait Algérie Tropical theileriosis cattle blood smear biochemistry haematology milk Algeria ????? ???????? ?????????? ??????? ??????? ??????????? ???????? ?????? ?????? ????????
??????? ???????? ??????? ??????Index. décimale : 636 Sciences Vétérinaires Résumé :
The present study carried out in the region of El Eulma (North-eastern Algeria) showed a highest prevalence (81.9%) of theileriosis (Theileria. annulata infection) among the piroplasmosis, the coinfection between theileriosis and babesiosis (B. bovis) are frequent (14.9%). The number of the highest cases (45) has parasitemia between 0.1 - 4.99%. A total of 180 and 134 blood samples from 21 farms were collected in April and November 2015 (before and after the tropical theileriosis season; respectively) in the region of El Eulma (Sétif). Molecular prevalence (38.9 and 50% in April and November; respectively) was higher than those by Giemsa stained blood smear (17 and 16.4%, before and after tropical theileriosis season; respectively) (p<0.001 ). Exotic breeds were more infected than local breeds in the first visit (p=0.003), while during the second visit no significant difference was detected (p=0.226). This prevalence is lower in cattle less than one year (p=0,117; 0,270 in April and November; respectively). Molecular prevalence was significantly higher in females (44.7%) than in males (22.9%) (p<0.008) during the first visit.
No influence of type of livestock or the walls statements was detected. The sequences of genes Algerian Tams1 (96.1 to 98.9%, identity values) have a relationship with the Mauritanian, Tunisian and Egyptian sequences.
The biochemical and haematological study conducted on 40 healthy cattle and 40 clinically infected showed that the number of leukocytes, erythrocytes, haemoglobin, haematocrit, glucose, albumin, and total protein decrease significantly (p=0.005; 0.008, 0.03,0.048, 0.002, 0.027 and 0.018; respectively) in clinically infected Montbeliard breed with Theileria annulata. All infected animals have a total and direct bilirubin concentration increase significantly (p<0.001). The erythrocytes, haemoglobin, and haematocrit crossbreed cattle concentration decrease significantly (p=0.027; 0.003 and <0.001; respectively). Two types of anaemia were detected: hypochromic microcytic anaemia in Montbeliard clinically infected cattle and normochromic normocytic anaemia in infected local and cross breed cattle.
The tropical theileriosis impact study in Skikda region (northern Algeria) showed a decrease in milk production in 10 local breed cattle infected with Theileria annulata in summer 2015. The decrease in weekly milk production estimated for two months was 2.76 L /day / cow corresponding to 31.92% of the total milk production. This decrease corresponds to 110.5 AD (US $ 1.02) / day / sick cow. The relative variation of milk production showed a dramatic decrease from 82.72 to 0.76% on day 21.
Diplôme : Doctorat en sciences En ligne : ../theses/veterinaire/AYA6981.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10414 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AYA/6981 AYA/6981 Thèse Bibliothèque principale Thèses Disponible
Titre : Contribution a l'étude des procèdes électrochimiques d'adoucissement des eaux dures Type de document : texte imprimé Auteurs : Yasmina Boulahèib (Née Bendaoud), Auteur ; Samira Ghizellaoui, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2015 Importance : 187 f. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Entartrage traitement chimique PCR chronoampérométrie impédancemétrie MEB KH2PO4 Polyphosphates HEDP Scaling chemical treatment chronoamperometry impedance SEM ???? ?????? ???????? ?????? ??????? ?????? ?????????????????? ?????? ????????? ???????? ????? Index. décimale : 540 Chimie et sciences connexes Résumé : Treating hard water has been interest from several works in order to reduce the adverse consequences of tartar. Hard water deposits form compact and adherent to the walls of pipes and industrial or domestic installations (GHIZELLAOUI, 2006).
Our study focuses on softening hard water Hamma having a hardness of 59°F and Negrine with a hardness of 56°F in the presence of KH2PO4, polyphosphates and HEDP.
The evaluation and the inhibition of scaling of hard waters of Hamma and Negrine were carried out by using four methods:
- The chemical method for evaluating the effective concentration of inhibitor to reduce the hardness of waters of Hamma and Negrine at temperature sources 30°C while maintaining a residual calcium concentration of 1 00 mg/L.
- The method of rapid precipitation controlled to track the phenomenon of germination growth during the formation of the deposit of calcium carbonate.
- The electrochemical method of accelerated scaling to assess the scaling power of raw waters of Hamma and Negrine by electrochemical way and in the presence of chemical inhibitors (KH2PO4, polyphosphates and HEDP );
- electrochemical method for measuring impedances of precipitated deposits metal deposit calcium carbonate
- electrolyte interface enables an overall assessment by means of the highfrequency resistance , adhesion and the importance of filing carbonate calcium ( Khalil et al.1993).
-The effect of inhibitors on the morphology and adhesion of the deposit of calcium carbonate was observed by the scanning electron microscope SEM and RX.
The application of chemical treatment of the waters of Hamma and Negrine at temperature of 30°C showed that partial inhibition of hardness requires addition of 2 mg/L of KH2PO4 for Hamma and 3 mg/L for Negrine. By against, the use of the polyphosphates inhibits scaling at a concentration of 10 mg/L for Hamma water and 5 mg/L for Negrine water.
The HEDP effect is at a concentration of 1 00 mg/L for Hamma and 50 mg/L for Negrine.
These values are reduced using the method of rapid controlled precipitation for each of the three inhibitors (0.1 mg/L KH2PO4, 0.01 mg/L Polyphosphates or 0.01 mg/L HEDP) for water of Hamma, and (0.5 mg/L KH2PO4, 1 mg/L Polyphosphates or 0.01 mg/L HEDP ) for water of Negrine.
The chronoamperometric study shows that the scaling time increases with the addition of (0.1 mg/L KH2PO4, 0.04 mg/L Polyphosphates or 0.005 mg/L HEDP ) for Hamma water and (0.5 mg/L KH2PO4, 0.25 mg/L Polyphosphtes or 10-5 mg/L HEDP) for Negrine water.
The impedance measurement obtained from the raw waters of Hamma and Negrine compared to treated waters shows a decrease in the amount of calcium carbonate scale as well as its adhesion by measuring high frequency resistors deposits from addition of (0.01 mg/L KH2PO4, 0.01 mg/L Polyphosphates or 0.005 mg/L HEDP) for Hamma also (0.5 mg/L KH2PO4, 0.05 mg/L Polyphosphates or 0.0075 mg/L HEDP) for Negrine.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/BOU6778.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9831 Contribution a l'étude des procèdes électrochimiques d'adoucissement des eaux dures [texte imprimé] / Yasmina Boulahèib (Née Bendaoud), Auteur ; Samira Ghizellaoui, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2015 . - 187 f.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Entartrage traitement chimique PCR chronoampérométrie impédancemétrie MEB KH2PO4 Polyphosphates HEDP Scaling chemical treatment chronoamperometry impedance SEM ???? ?????? ???????? ?????? ??????? ?????? ?????????????????? ?????? ????????? ???????? ????? Index. décimale : 540 Chimie et sciences connexes Résumé : Treating hard water has been interest from several works in order to reduce the adverse consequences of tartar. Hard water deposits form compact and adherent to the walls of pipes and industrial or domestic installations (GHIZELLAOUI, 2006).
Our study focuses on softening hard water Hamma having a hardness of 59°F and Negrine with a hardness of 56°F in the presence of KH2PO4, polyphosphates and HEDP.
The evaluation and the inhibition of scaling of hard waters of Hamma and Negrine were carried out by using four methods:
- The chemical method for evaluating the effective concentration of inhibitor to reduce the hardness of waters of Hamma and Negrine at temperature sources 30°C while maintaining a residual calcium concentration of 1 00 mg/L.
- The method of rapid precipitation controlled to track the phenomenon of germination growth during the formation of the deposit of calcium carbonate.
- The electrochemical method of accelerated scaling to assess the scaling power of raw waters of Hamma and Negrine by electrochemical way and in the presence of chemical inhibitors (KH2PO4, polyphosphates and HEDP );
- electrochemical method for measuring impedances of precipitated deposits metal deposit calcium carbonate
- electrolyte interface enables an overall assessment by means of the highfrequency resistance , adhesion and the importance of filing carbonate calcium ( Khalil et al.1993).
-The effect of inhibitors on the morphology and adhesion of the deposit of calcium carbonate was observed by the scanning electron microscope SEM and RX.
The application of chemical treatment of the waters of Hamma and Negrine at temperature of 30°C showed that partial inhibition of hardness requires addition of 2 mg/L of KH2PO4 for Hamma and 3 mg/L for Negrine. By against, the use of the polyphosphates inhibits scaling at a concentration of 10 mg/L for Hamma water and 5 mg/L for Negrine water.
The HEDP effect is at a concentration of 1 00 mg/L for Hamma and 50 mg/L for Negrine.
These values are reduced using the method of rapid controlled precipitation for each of the three inhibitors (0.1 mg/L KH2PO4, 0.01 mg/L Polyphosphates or 0.01 mg/L HEDP) for water of Hamma, and (0.5 mg/L KH2PO4, 1 mg/L Polyphosphates or 0.01 mg/L HEDP ) for water of Negrine.
The chronoamperometric study shows that the scaling time increases with the addition of (0.1 mg/L KH2PO4, 0.04 mg/L Polyphosphates or 0.005 mg/L HEDP ) for Hamma water and (0.5 mg/L KH2PO4, 0.25 mg/L Polyphosphtes or 10-5 mg/L HEDP) for Negrine water.
The impedance measurement obtained from the raw waters of Hamma and Negrine compared to treated waters shows a decrease in the amount of calcium carbonate scale as well as its adhesion by measuring high frequency resistors deposits from addition of (0.01 mg/L KH2PO4, 0.01 mg/L Polyphosphates or 0.005 mg/L HEDP) for Hamma also (0.5 mg/L KH2PO4, 0.05 mg/L Polyphosphates or 0.0075 mg/L HEDP) for Negrine.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/BOU6778.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9831 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/6778 BOU/6778 Thèse Bibliothèque principale Thèses Disponible
Titre : Diagnostic moléculaire des amyotrophies spinales : recherche de la délétion de l'exon 8 du gène SMN Type de document : texte imprimé Auteurs : Université Mentouri, Editeur scientifique Année de publication : 2008 Importance : 116 f. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : PCR SMA GLne SMA Exon 8 Exon 7 Délétion Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/HAM5158.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1799 Diagnostic moléculaire des amyotrophies spinales : recherche de la délétion de l'exon 8 du gène SMN [texte imprimé] / Université Mentouri, Editeur scientifique . - 2008 . - 116 f.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : PCR SMA GLne SMA Exon 8 Exon 7 Délétion Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/HAM5158.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1799 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité HAM/5158 HAM/5158 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude des Leishmanioses diagnostiquées au centre hospitalo-universitaire Ben Badis de Constantine Type de document : texte imprimé Auteurs : Université Mentouri, Editeur scientifique Année de publication : 2006 Importance : 127 f. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Diagnostic Constantine PCR Leishmaniose CUTAN2E . Leishmaniose viscérale Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/DJE4726.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1349 Etude des Leishmanioses diagnostiquées au centre hospitalo-universitaire Ben Badis de Constantine [texte imprimé] / Université Mentouri, Editeur scientifique . - 2006 . - 127 f.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Diagnostic Constantine PCR Leishmaniose CUTAN2E . Leishmaniose viscérale Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/DJE4726.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1349 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité DJE/4726 DJE/4726 Thèse Bibliothèque principale Thèses Disponible
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