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Auteur Dalila Satta |
Documents disponibles écrits par cet auteur (6)
Affiner la recherche Interroger des sources externesAspects biologiques et génétiques des leucémies aigues Implication des gènes FLT3 et NPM1 dans la réponse au traitement. / Ouarda Sariyah Ayachi
Titre : Aspects biologiques et génétiques des leucémies aigues Implication des gènes FLT3 et NPM1 dans la réponse au traitement. Type de document : texte imprimé Auteurs : Ouarda Sariyah Ayachi, Auteur ; Dalila Satta, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 138 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie Animale:Génétique Moléculaire leucémies aigues mutations FLT3 NPM1 survie globale acute leukemia FLT3 mutations NPM1 mutations survival الوكيميا الحادة الطفرات الوراثية FLT3-ITD TKD Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Leukemia is a very heterogeneous group of malignant homeopathies and this heterogeneousness expresses itself more particularly in the acute leukemia where sometimes it turns out to be difficult to make the distinction between the various entities. The physiopathology of this disease is starting to be understood at the cellular and molecular levels, and cytogenetic markers have become the most important for the stratification of the risk and the treatment of the patients. However, with the advent of the new technologies, the identification of other molecular markers, like gene mutations and the characterization of the profiles of the epigenetic modifications, began to play an important role in the understanding of the disease. The identification of these biomarkers contributes to a better understanding and a better
treatment strategies of the patients. At first of our work, we proceeded to a transverse study on the acute leukaemia, and for that we made a hematological, cytological and cytogenetic analysis for acute leukemia patients who were received at hematology center in our hospital. Secondly, molecular variations of the gene FLT3 (FLT3-ITD and FLT3-TKD) and of the exon12 of the gene NPM1 were analysed for the AML patients. Our results reveal that FLT3 and NPM1 mutations are less frequent in our population than reported in literature. we were also able to describe a new unreported complex mutation in the exon 12 of the NPM1 gene. Finally, we analyzed the effect of the presence of the previously detected mutations on the answer to the treatment, the survival and the future of the AML patients. Our results showed that the mutations of the FLT3 have a pejorative effect on the future of the patients, they indeed increase, the possibility relapse and death rate contrary to the mutations of the NPM1 gene which appear to have a positive effect on the AML patients when they are not associated with the FLT3-ITDs. The molecular and cytogenetic analysis for leukemia patients’ turned out to be an important diagnosis tool for prognosis of the acute leukemia. The specific association between acute leukemia subtypes and certain anomalies was clearly established. These analyses are important for confirming the diagnosis, to establish a prognosis, to make a choice and a therapeutic follow-up and consequently, the management of patients at the hematological centers can be improved.Note de contenu :
Annexes.
Diplôme : Doctorat En ligne : ../theses/biologie/AYA7454.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11239 Aspects biologiques et génétiques des leucémies aigues Implication des gènes FLT3 et NPM1 dans la réponse au traitement. [texte imprimé] / Ouarda Sariyah Ayachi, Auteur ; Dalila Satta, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2019 . - 138 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie Animale:Génétique Moléculaire leucémies aigues mutations FLT3 NPM1 survie globale acute leukemia FLT3 mutations NPM1 mutations survival الوكيميا الحادة الطفرات الوراثية FLT3-ITD TKD Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Leukemia is a very heterogeneous group of malignant homeopathies and this heterogeneousness expresses itself more particularly in the acute leukemia where sometimes it turns out to be difficult to make the distinction between the various entities. The physiopathology of this disease is starting to be understood at the cellular and molecular levels, and cytogenetic markers have become the most important for the stratification of the risk and the treatment of the patients. However, with the advent of the new technologies, the identification of other molecular markers, like gene mutations and the characterization of the profiles of the epigenetic modifications, began to play an important role in the understanding of the disease. The identification of these biomarkers contributes to a better understanding and a better
treatment strategies of the patients. At first of our work, we proceeded to a transverse study on the acute leukaemia, and for that we made a hematological, cytological and cytogenetic analysis for acute leukemia patients who were received at hematology center in our hospital. Secondly, molecular variations of the gene FLT3 (FLT3-ITD and FLT3-TKD) and of the exon12 of the gene NPM1 were analysed for the AML patients. Our results reveal that FLT3 and NPM1 mutations are less frequent in our population than reported in literature. we were also able to describe a new unreported complex mutation in the exon 12 of the NPM1 gene. Finally, we analyzed the effect of the presence of the previously detected mutations on the answer to the treatment, the survival and the future of the AML patients. Our results showed that the mutations of the FLT3 have a pejorative effect on the future of the patients, they indeed increase, the possibility relapse and death rate contrary to the mutations of the NPM1 gene which appear to have a positive effect on the AML patients when they are not associated with the FLT3-ITDs. The molecular and cytogenetic analysis for leukemia patients’ turned out to be an important diagnosis tool for prognosis of the acute leukemia. The specific association between acute leukemia subtypes and certain anomalies was clearly established. These analyses are important for confirming the diagnosis, to establish a prognosis, to make a choice and a therapeutic follow-up and consequently, the management of patients at the hematological centers can be improved.Note de contenu :
Annexes.
Diplôme : Doctorat En ligne : ../theses/biologie/AYA7454.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11239 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AYA/7454 AYA/7454 Thèse Bibliothèque principale Thèses Disponible
Titre : Étude des gènes impliqués dans l’apparition du cancer du poumon. Type de document : texte imprimé Auteurs : Asma Mimouni, Auteur ; Dalila Satta, Directeur de thèse Mention d'édition : 05/10/2021 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2021 Importance : 123 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie Animale:Génétique Moléculaire Cancer bronchique CHRNA5 Est algérien, facteurs de risque OGG1 prédisposition TERT Lung cancer predisposition Est Algerian Risk Factor سرطان الرئة القابلية للمرض الشرق الجزائري، CHRNA5عامل الخطر Index. décimale : 570 Sciences de la vie. Biologie Résumé : Due to its late discovery, bronchopulmonary cancer is a frequent cancer and poorly prognosticated, it may become a major health problem. Improving our knowledge of this cancer has become a major obligation in order to improve patient care with the implementation of appropriate targeted therapy. In this context, we carried out an epidemiological study of the case-control type in order to study the association of certain risk factors and CB in the EastAlgerian population. Then in a second part of our work, we carried out the molecular analysis of three polymorphisms of the TERT, OGG1 and CHRNA5 genes, with the aim of elucidating whether these polymorphisms predispose the population of eastern Algeria to lung cancer not to small cells (NSCLC). To date, no study has considered this association in our population. Materials and methods: The study included 211 healthy people and 169 cases with BC. Genotyping was performed using TaqMan probes and Sanger sequencing. Data were analyzed by multivariate logistic regression analysis adjusted for covariates. Results: Of the cases studied, 84% were men and 16% women. The majority of patients (94.4%) were diagnosed at advanced stages of the disease, with the predominant symptoms being chest pain, cough, dyspnea, and hemoptysis. Smokers represented 77.3% of cases with a rate of 43.36 packs / year. The mean age at diagnosis was 59.9 years (26 and 80 years) with 55% of our patients having ADK, 28.4% squamous cell carcinoma and 1.8% small cell lung cancer. 8.2% of our patients underwent surgery. The minor allele frequencies (MAFs) of TERT rs2853669, CHRNA5 rs16969968 and OGG1 polymorphisms in controls were C: 20%, A: 31% and G: 29%, respectively. Of the three polymorphisms, none shows a significant association, but the stratified rs1052133analysis rs16969968 showed that people carrying the AA genotype are significantly associated with the risk of adenocarcinoma (pAdj = 0.03, ORAdj = 2.55). Smokers with an AA allele have a greater risk of lung cancer than those with genotype GG or GA (pAdj = 0.03 ORAdj = 3.91), which is not the case for non-smokers. Conclusion: Our study suggests that the CHRNA5 rs16969968 polymorphism is associated with a significant increased risk of pulmonary adenocarcinoma and nicotine dependence.
Note de contenu : Annexes. Diplôme : Doctorat En ligne : ../theses/biologie/MIM7822.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11654 Étude des gènes impliqués dans l’apparition du cancer du poumon. [texte imprimé] / Asma Mimouni, Auteur ; Dalila Satta, Directeur de thèse . - 05/10/2021 . - جامعة الإخوة منتوري قسنطينة, 2021 . - 123 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie Animale:Génétique Moléculaire Cancer bronchique CHRNA5 Est algérien, facteurs de risque OGG1 prédisposition TERT Lung cancer predisposition Est Algerian Risk Factor سرطان الرئة القابلية للمرض الشرق الجزائري، CHRNA5عامل الخطر Index. décimale : 570 Sciences de la vie. Biologie Résumé : Due to its late discovery, bronchopulmonary cancer is a frequent cancer and poorly prognosticated, it may become a major health problem. Improving our knowledge of this cancer has become a major obligation in order to improve patient care with the implementation of appropriate targeted therapy. In this context, we carried out an epidemiological study of the case-control type in order to study the association of certain risk factors and CB in the EastAlgerian population. Then in a second part of our work, we carried out the molecular analysis of three polymorphisms of the TERT, OGG1 and CHRNA5 genes, with the aim of elucidating whether these polymorphisms predispose the population of eastern Algeria to lung cancer not to small cells (NSCLC). To date, no study has considered this association in our population. Materials and methods: The study included 211 healthy people and 169 cases with BC. Genotyping was performed using TaqMan probes and Sanger sequencing. Data were analyzed by multivariate logistic regression analysis adjusted for covariates. Results: Of the cases studied, 84% were men and 16% women. The majority of patients (94.4%) were diagnosed at advanced stages of the disease, with the predominant symptoms being chest pain, cough, dyspnea, and hemoptysis. Smokers represented 77.3% of cases with a rate of 43.36 packs / year. The mean age at diagnosis was 59.9 years (26 and 80 years) with 55% of our patients having ADK, 28.4% squamous cell carcinoma and 1.8% small cell lung cancer. 8.2% of our patients underwent surgery. The minor allele frequencies (MAFs) of TERT rs2853669, CHRNA5 rs16969968 and OGG1 polymorphisms in controls were C: 20%, A: 31% and G: 29%, respectively. Of the three polymorphisms, none shows a significant association, but the stratified rs1052133analysis rs16969968 showed that people carrying the AA genotype are significantly associated with the risk of adenocarcinoma (pAdj = 0.03, ORAdj = 2.55). Smokers with an AA allele have a greater risk of lung cancer than those with genotype GG or GA (pAdj = 0.03 ORAdj = 3.91), which is not the case for non-smokers. Conclusion: Our study suggests that the CHRNA5 rs16969968 polymorphism is associated with a significant increased risk of pulmonary adenocarcinoma and nicotine dependence.
Note de contenu : Annexes. Diplôme : Doctorat En ligne : ../theses/biologie/MIM7822.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11654 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MIM/7822 MIM/7822 Thèse Bibliothèque principale Thèses Disponible
Titre : Étude génétique des gliomes : étude des polymorphismes C677T de la MTHFR et I/D de l’ACE chez une population de l’Est algérien atteinte de gliome. Type de document : texte imprimé Auteurs : Ikram Hana Benenemissi, Auteur ; Dalila Satta, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 133 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie Animale: Génétique Moléculaire gliomes polymorphisme C677T de la MTHFR polymorphisme I/D de l’ACE C677T polymorphism of MTHFR I/D ACE polymorphism الأورام الدبقية التعدد الشكلي MTHFE C677Tالتعدد الشكلي Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Gliomas are the most common primary intraparenchymal tumors of the central nervous system with a very poor prognosis. Glioblastoma accounts for the majority of gliomas (56,6%). Relative survival estimates for glioblastoma are quite low; 5.6% of patients survived five years post diagnosis. Several evidences demonstrate the importance role of genetic factors in glioma development. The evolution of cytogenetic and molecular biology researches, has largely elucidated the molecular mechanisms of gliomagenesis, which have a lot of impact in diagnosis, prognosis, and therapeutic to predict responses to different therapies proposed. Our case-control study will focus on two genetic polymorphisms: The C677T polymorphism of MTHFR and the I / D polymorphism of ACE on blood sample. The results obtained was:
the genotype TT of C677T MTHFR polymorphim is highly associated with high genesis of glioma in East-Algerian population. The presence of T allele may contribute to development of glioma in this population. the DD genotype of I/D ACE polymorphism in associated with high risk to develop glioma in our population. These results allow us to propose the study of these genes for therapeutic (use of ACE inhibitors and folate substitution) and deserve confirmation by the realization of other studies involving a larger sample of glioma patients.Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/biologie/BEN7525.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11365 Étude génétique des gliomes : étude des polymorphismes C677T de la MTHFR et I/D de l’ACE chez une population de l’Est algérien atteinte de gliome. [texte imprimé] / Ikram Hana Benenemissi, Auteur ; Dalila Satta, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2019 . - 133 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie Animale: Génétique Moléculaire gliomes polymorphisme C677T de la MTHFR polymorphisme I/D de l’ACE C677T polymorphism of MTHFR I/D ACE polymorphism الأورام الدبقية التعدد الشكلي MTHFE C677Tالتعدد الشكلي Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Gliomas are the most common primary intraparenchymal tumors of the central nervous system with a very poor prognosis. Glioblastoma accounts for the majority of gliomas (56,6%). Relative survival estimates for glioblastoma are quite low; 5.6% of patients survived five years post diagnosis. Several evidences demonstrate the importance role of genetic factors in glioma development. The evolution of cytogenetic and molecular biology researches, has largely elucidated the molecular mechanisms of gliomagenesis, which have a lot of impact in diagnosis, prognosis, and therapeutic to predict responses to different therapies proposed. Our case-control study will focus on two genetic polymorphisms: The C677T polymorphism of MTHFR and the I / D polymorphism of ACE on blood sample. The results obtained was:
the genotype TT of C677T MTHFR polymorphim is highly associated with high genesis of glioma in East-Algerian population. The presence of T allele may contribute to development of glioma in this population. the DD genotype of I/D ACE polymorphism in associated with high risk to develop glioma in our population. These results allow us to propose the study of these genes for therapeutic (use of ACE inhibitors and folate substitution) and deserve confirmation by the realization of other studies involving a larger sample of glioma patients.Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/biologie/BEN7525.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11365 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEN/7525 BEN/7525 Thèse Bibliothèque principale Thèses Disponible
Titre : Etudes comparatives des souches d'escherichia coli aviares et humaines Type de document : texte imprimé Auteurs : Nawel Lezzar, Auteur ; Dalila Satta, Directeur de thèse ; Charaf Chebbah, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 311 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
Sciences VétérinairesTags : Escherichia coli Souches d’E. coli Aviaires Souches d’E. coli Humaines Antibiorésistance Prévention "ميكروب الآي كولاي القولونية سلالات القولونية من الطيور سلالات القولونية من الإنسان مقاومة المضادات الحيوية الوقاية Index. décimale : 636 Sciences Vétérinaires Résumé : Escherichia coli infections or colibacillosis continue to be always a major cause of economic losses for
poultry farms. Apart from the pathogens of Escherichia coli that are responsible for it, it is important
to do shed light on the strains Escherichia coli saprophytes antibiotic-resistant wich are responsible
for therapeutic failures and wich are representing the main source of the spread of this antibiotic
resistance, through resistant genes, thus ensuring their transmission to other saprophytic agents and
pathogenic in humans as well as in poultry (in particular) and animals (in general).
In this study, we show the emergence of a multiresistance through the resistance profile of two
collections of E. coli poultry Experimental (ELEX1 and ELEX2) to the 6 Quinolones tested
(Flumequine (UB), Pefloxacin (PEF), Norfloxacin (NOR), Enrofloxacin (EN), Ciprofloxacin (CIP),
and experimental avian influenza vaccines (ELEX1 and ELEX2), as well as a multiresistance through
the resistance profile of two collections of E. coli Poultry and Humain to the 40 antibiotics tested
(Ampicillin (AMX), Piperacillin (PRL), Ticarcillin (TC), Amoxicillin / Clavulanic Acid (AMC),
Cefalexin (CN30), Cefazoline (CZ), Cefalotine (CF) Cefepaxime (CTX), Cefotaxime (CRT),
Cefitaxone (FOX), Ceftazidime (CAZ), Cefixime (CFM), Aztreonam ), Gentamycin (CN10),
Tobramycin (TOB), Amikacin (AK), Kanamycin (K), Netilmicin (NET), Streptomycin (S),
Tetracycline (TE), Doxyciline (DXT), Nalidixic Acid NA), Flumequine (UB), Pefloxacin (PEF),
Norfloxacin (NOR), Ofloxacin (OFX), Ciprofloxacin (CIP), Sulfonamides (SSS), Trimethoprim
(TMP), Trimethoprim / Sulfamethoxazole Chloramphenicol (C), Fosfomycin (FF), Nitofurantoines
(F)).
Out of a total of 2635 samples, of which 264 were taken from the two experimental poultry farms (108
for ELEX1 and 156 for ELEX2), 600 collected from the 12 broiler farms (from the 06 communes of
the wilaya of Constantine) and 1771 collected from patients of CHU of Constantine (hospital and
ambulatory), we obtained 291 of antibiotic-resistant E. Coli strains, including 46 antibiotic-resistant E.
Coli strains to the 06 quinolones (for the two collections of E. coli Experimental), 120/209 of E. coli
strains (for E. coli aviary strains) and 125 of E. coli strains (for E. coli Human strains) to the 40
antibiotics tested.
From the different resistance profiles obtained, the emergence of several resistance profiles follow a
selection pressure for antibiotics by misuse of the latter in veterinary medicine and in human medicine
(upstream) but also to the dissemination of resistance genes by failure to respect hygienic measures
(downstream) and which remain the business of all.Therefore antibiotic resistance monitoring is
required at all levels and at all times by the systematic application of ""broad-spectrum antibiogram »
on commensal and pathogenic bacterial populations.
Diplôme : Doctorat en sciences En ligne : ../theses/veterinaire/LEZ7153.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10434 Etudes comparatives des souches d'escherichia coli aviares et humaines [texte imprimé] / Nawel Lezzar, Auteur ; Dalila Satta, Directeur de thèse ; Charaf Chebbah, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 311 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
Sciences VétérinairesTags : Escherichia coli Souches d’E. coli Aviaires Souches d’E. coli Humaines Antibiorésistance Prévention "ميكروب الآي كولاي القولونية سلالات القولونية من الطيور سلالات القولونية من الإنسان مقاومة المضادات الحيوية الوقاية Index. décimale : 636 Sciences Vétérinaires Résumé : Escherichia coli infections or colibacillosis continue to be always a major cause of economic losses for
poultry farms. Apart from the pathogens of Escherichia coli that are responsible for it, it is important
to do shed light on the strains Escherichia coli saprophytes antibiotic-resistant wich are responsible
for therapeutic failures and wich are representing the main source of the spread of this antibiotic
resistance, through resistant genes, thus ensuring their transmission to other saprophytic agents and
pathogenic in humans as well as in poultry (in particular) and animals (in general).
In this study, we show the emergence of a multiresistance through the resistance profile of two
collections of E. coli poultry Experimental (ELEX1 and ELEX2) to the 6 Quinolones tested
(Flumequine (UB), Pefloxacin (PEF), Norfloxacin (NOR), Enrofloxacin (EN), Ciprofloxacin (CIP),
and experimental avian influenza vaccines (ELEX1 and ELEX2), as well as a multiresistance through
the resistance profile of two collections of E. coli Poultry and Humain to the 40 antibiotics tested
(Ampicillin (AMX), Piperacillin (PRL), Ticarcillin (TC), Amoxicillin / Clavulanic Acid (AMC),
Cefalexin (CN30), Cefazoline (CZ), Cefalotine (CF) Cefepaxime (CTX), Cefotaxime (CRT),
Cefitaxone (FOX), Ceftazidime (CAZ), Cefixime (CFM), Aztreonam ), Gentamycin (CN10),
Tobramycin (TOB), Amikacin (AK), Kanamycin (K), Netilmicin (NET), Streptomycin (S),
Tetracycline (TE), Doxyciline (DXT), Nalidixic Acid NA), Flumequine (UB), Pefloxacin (PEF),
Norfloxacin (NOR), Ofloxacin (OFX), Ciprofloxacin (CIP), Sulfonamides (SSS), Trimethoprim
(TMP), Trimethoprim / Sulfamethoxazole Chloramphenicol (C), Fosfomycin (FF), Nitofurantoines
(F)).
Out of a total of 2635 samples, of which 264 were taken from the two experimental poultry farms (108
for ELEX1 and 156 for ELEX2), 600 collected from the 12 broiler farms (from the 06 communes of
the wilaya of Constantine) and 1771 collected from patients of CHU of Constantine (hospital and
ambulatory), we obtained 291 of antibiotic-resistant E. Coli strains, including 46 antibiotic-resistant E.
Coli strains to the 06 quinolones (for the two collections of E. coli Experimental), 120/209 of E. coli
strains (for E. coli aviary strains) and 125 of E. coli strains (for E. coli Human strains) to the 40
antibiotics tested.
From the different resistance profiles obtained, the emergence of several resistance profiles follow a
selection pressure for antibiotics by misuse of the latter in veterinary medicine and in human medicine
(upstream) but also to the dissemination of resistance genes by failure to respect hygienic measures
(downstream) and which remain the business of all.Therefore antibiotic resistance monitoring is
required at all levels and at all times by the systematic application of ""broad-spectrum antibiogram »
on commensal and pathogenic bacterial populations.
Diplôme : Doctorat en sciences En ligne : ../theses/veterinaire/LEZ7153.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10434 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LEZ/7153 LEZ/7153 Thèse Bibliothèque principale Thèses Disponible
Titre : Génétique mitochondriale des infertilités masculines Type de document : texte imprimé Auteurs : Mohamed Larbi Rezgoune, Auteur ; Dalila Satta, Directeur de thèse ; Abdelhamid Slama, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 187 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : mitochondrie polymorphisme infertilité masculine biologie moléculaire mitochondria polymorphism male infertility molecular biology الميتوكوندري تعدد الاشكال الجيني اصطرابات الخصوبة عند الرجال البيولوجيا الجزيئية Index. décimale : 570 Sciences de la vie. Biologie Résumé : Mitochondria play a central role in energy metabolism, homeostasis and apoptosis.
These functions are in part under the control of a mitochondrial genome, support of cytoplasmic heredity, whose transmission is only maternal. The involvement of these organelles in human reproduction is a relatively new concept that arouses scientific and medical interest. Abnormalities both qualitative and quantitative of mitochondrial DNA (mtDNA) have been associated with male fertility disorders.
Infertility concerns 10 to 15 % of couples wishing to have a child, a male component is found
in almost half of cases. In a significant proportion of these cases, a known genetic basis,
chromosomal or gene, sometimes transmitted from the parents, is involved.
In this work, in a first part, different genetic polymorphisms in humans: (CAG)n POLG1,T3801C CYP1A1 and MTHFR A1298C, and their role as potential risk factors for male infertility in an Algerian population cohort were studied by sequencing or RFLP.
Our results show that the variants described at the MTHFR and CYP1A1 genes are not associated with male infertility unlike that of POLG1 which appears to be involved in this dysfunction.
In the second part of our work, we have prospected qualitative alterations of mitochondrial
genome by analysis of macro-deletions by long PCR and quantitative by quantification of leukocyte mtDNA rate by real-time quantitative PCR (mitochondrial DNA vs nuclear) in idiopathic male infertility. The mtDNA deletion does not appear to be associated with this disorder but the assessment of the level of leukocyte mtDNA likely suggests the involvement of this parameter in male infertility especially in azoospermic and asthenospermic men.
Molecular biology has led to the discovery of unexplored etiologies until now without denigrating the clinical and biological explanatory value of many recognized etiologies.
This mitochondrial molecular appearance misunderstood opens up new perspectives and may in the future introduce profound changes in the genetic approach to male infertility.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/REZ7000.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10389 Génétique mitochondriale des infertilités masculines [texte imprimé] / Mohamed Larbi Rezgoune, Auteur ; Dalila Satta, Directeur de thèse ; Abdelhamid Slama, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 187 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : mitochondrie polymorphisme infertilité masculine biologie moléculaire mitochondria polymorphism male infertility molecular biology الميتوكوندري تعدد الاشكال الجيني اصطرابات الخصوبة عند الرجال البيولوجيا الجزيئية Index. décimale : 570 Sciences de la vie. Biologie Résumé : Mitochondria play a central role in energy metabolism, homeostasis and apoptosis.
These functions are in part under the control of a mitochondrial genome, support of cytoplasmic heredity, whose transmission is only maternal. The involvement of these organelles in human reproduction is a relatively new concept that arouses scientific and medical interest. Abnormalities both qualitative and quantitative of mitochondrial DNA (mtDNA) have been associated with male fertility disorders.
Infertility concerns 10 to 15 % of couples wishing to have a child, a male component is found
in almost half of cases. In a significant proportion of these cases, a known genetic basis,
chromosomal or gene, sometimes transmitted from the parents, is involved.
In this work, in a first part, different genetic polymorphisms in humans: (CAG)n POLG1,T3801C CYP1A1 and MTHFR A1298C, and their role as potential risk factors for male infertility in an Algerian population cohort were studied by sequencing or RFLP.
Our results show that the variants described at the MTHFR and CYP1A1 genes are not associated with male infertility unlike that of POLG1 which appears to be involved in this dysfunction.
In the second part of our work, we have prospected qualitative alterations of mitochondrial
genome by analysis of macro-deletions by long PCR and quantitative by quantification of leukocyte mtDNA rate by real-time quantitative PCR (mitochondrial DNA vs nuclear) in idiopathic male infertility. The mtDNA deletion does not appear to be associated with this disorder but the assessment of the level of leukocyte mtDNA likely suggests the involvement of this parameter in male infertility especially in azoospermic and asthenospermic men.
Molecular biology has led to the discovery of unexplored etiologies until now without denigrating the clinical and biological explanatory value of many recognized etiologies.
This mitochondrial molecular appearance misunderstood opens up new perspectives and may in the future introduce profound changes in the genetic approach to male infertility.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/REZ7000.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10389 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité REZ/7000 REZ/7000 Thèse Bibliothèque principale Thèses Disponible
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