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Partager le résultat de cette recherche Interroger des sources externesIsolement de souches microbiennes à partir de biotopes algérien et mise en évidence de la production de pigments caroténoïdes / Wissem Boutana
Titre : Isolement de souches microbiennes à partir de biotopes algérien et mise en évidence de la production de pigments caroténoïdes Type de document : texte imprimé Auteurs : Wissem Boutana, Auteur ; Farida Bekhouche, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 91 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Biopigments caroténoïdes souches pigmentées isolement carotenoids pigmented strains isolation الاصباغ الحیویة الكاروتینات السلالات الصباغیة العزلة Index. décimale : 640 Industrie Alimentaire Résumé : The use of microorganisms for the production of natural pigments is considered to be
one of the fastest growing pathways in biotechnology.The considerable interest in
biopigments, in particular carotenoids, is linked to their use as additives to coloring properties
in the food industry and also thanks to their provitaminic (vitamin A), antioxidant and
antiradical properties.The aim of our work is the isolation of pigmented strains from Algerian
biotopes (molasses, margines, whey and olives) and to test their carotenoid pigment synthesis
capacities. The creamy white, yellow, orange and pink colors served for the first selection of
120 pigmented strains of which 89 are bacteria and 21 are yeasts. The second selection
resulted in 40 strains which were used to demonstrate the production of pigments in liquid
media (TSB, YMB, OGB and SDB) the bacterial group consisting of 27 isolates and the yeast
group represented by 13 isolates. Measurements of pH, amount of biomass and carotenoid
pigment content revealed no significant variability between the last two parameters. The
pigment production test revealed that the BMY108 strain is potentially producing carotenoids
(1.79 ± 0.33 mg of equivalent β-carotène/g MS) and this on synthetic medium. These findings
open the possibilities of alternatives such as the use of low-cost substrates as agro-industrial
waste for the improvement of production.
Note de contenu :
Diplôme : Magistère En ligne : ../theses/agronomie/BOU7040.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10560 Isolement de souches microbiennes à partir de biotopes algérien et mise en évidence de la production de pigments caroténoïdes [texte imprimé] / Wissem Boutana, Auteur ; Farida Bekhouche, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 91 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Biopigments caroténoïdes souches pigmentées isolement carotenoids pigmented strains isolation الاصباغ الحیویة الكاروتینات السلالات الصباغیة العزلة Index. décimale : 640 Industrie Alimentaire Résumé : The use of microorganisms for the production of natural pigments is considered to be
one of the fastest growing pathways in biotechnology.The considerable interest in
biopigments, in particular carotenoids, is linked to their use as additives to coloring properties
in the food industry and also thanks to their provitaminic (vitamin A), antioxidant and
antiradical properties.The aim of our work is the isolation of pigmented strains from Algerian
biotopes (molasses, margines, whey and olives) and to test their carotenoid pigment synthesis
capacities. The creamy white, yellow, orange and pink colors served for the first selection of
120 pigmented strains of which 89 are bacteria and 21 are yeasts. The second selection
resulted in 40 strains which were used to demonstrate the production of pigments in liquid
media (TSB, YMB, OGB and SDB) the bacterial group consisting of 27 isolates and the yeast
group represented by 13 isolates. Measurements of pH, amount of biomass and carotenoid
pigment content revealed no significant variability between the last two parameters. The
pigment production test revealed that the BMY108 strain is potentially producing carotenoids
(1.79 ± 0.33 mg of equivalent β-carotène/g MS) and this on synthetic medium. These findings
open the possibilities of alternatives such as the use of low-cost substrates as agro-industrial
waste for the improvement of production.
Note de contenu :
Diplôme : Magistère En ligne : ../theses/agronomie/BOU7040.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10560 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/7040 BOU/7040 Thèse Bibliothèque principale Thèses Disponible
Titre : Isolement et caractérisation de souches fongiques de milieux extremes (sol et sebkha de la région de Biskra) productrices de cellulase themostable à interet industriel Type de document : texte imprimé Auteurs : Soumia Bensmira, Auteur ; Zahia Meraihi, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2006 Importance : 88 f. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Déchets de dattes Identification Thermostabilité Cellulase Isolemen Milieux extremes Moisissures Isolation extreme medium fungi date scrap cellulase thermostability عزل تعريف وسط اقصى بقايا التمور سلولاز مقاومة الحرارة Index. décimale : 570 Sciences de la vie. Biologie Résumé : The microscopic study of the moulds isolated from Sebkhas of Biskra area allowed, to identify 7 different kinds from 10 potentially cellulolytic strains: Aspergillus, Penicillium, Geotrichum, Altarnaria, Cladosporium, Altarnaria and Trichoderma.
The fermentation of the 10 strains selected and cultivated on a medium containing date scrap (under product of pheonicical industry) as a substrate of fermentation, allowed to select hypercellulolytic strains with a cellulasic activity of 775 U. The strain (R3), isolated from the ground soil of palm plantations of Biskra corresponds to the Trichoderma kind. This train is retained for the kinetic study of the biomass, the cellulasic activity, sugar, proteins like that of the pH. This study reveals that the synthesis of the enzyme reaches its maximum after 60 hours of fermentation during the stationary phase of the growth.
A partial purification with ammonia sulphate 40 % of saturetion reduced the content of proteins of 78.4 % and increased the specific activity to 94.35 % which reached the degree of purification to 17.70. The enzyme was found very thermostable with a half-life of 170 , 140, 120 and 50 munites at 60, 70, 80 and 90°C respectively. The corresponding temperature for the maximum cellulolytic activity was 55°C whereas the optimim pH was 4.8.Diplôme : Magistère En ligne : ../theses/biologie/BEN4503.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1348 Isolement et caractérisation de souches fongiques de milieux extremes (sol et sebkha de la région de Biskra) productrices de cellulase themostable à interet industriel [texte imprimé] / Soumia Bensmira, Auteur ; Zahia Meraihi, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2006 . - 88 f.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C.
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Déchets de dattes Identification Thermostabilité Cellulase Isolemen Milieux extremes Moisissures Isolation extreme medium fungi date scrap cellulase thermostability عزل تعريف وسط اقصى بقايا التمور سلولاز مقاومة الحرارة Index. décimale : 570 Sciences de la vie. Biologie Résumé : The microscopic study of the moulds isolated from Sebkhas of Biskra area allowed, to identify 7 different kinds from 10 potentially cellulolytic strains: Aspergillus, Penicillium, Geotrichum, Altarnaria, Cladosporium, Altarnaria and Trichoderma.
The fermentation of the 10 strains selected and cultivated on a medium containing date scrap (under product of pheonicical industry) as a substrate of fermentation, allowed to select hypercellulolytic strains with a cellulasic activity of 775 U. The strain (R3), isolated from the ground soil of palm plantations of Biskra corresponds to the Trichoderma kind. This train is retained for the kinetic study of the biomass, the cellulasic activity, sugar, proteins like that of the pH. This study reveals that the synthesis of the enzyme reaches its maximum after 60 hours of fermentation during the stationary phase of the growth.
A partial purification with ammonia sulphate 40 % of saturetion reduced the content of proteins of 78.4 % and increased the specific activity to 94.35 % which reached the degree of purification to 17.70. The enzyme was found very thermostable with a half-life of 170 , 140, 120 and 50 munites at 60, 70, 80 and 90°C respectively. The corresponding temperature for the maximum cellulolytic activity was 55°C whereas the optimim pH was 4.8.Diplôme : Magistère En ligne : ../theses/biologie/BEN4503.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1348 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEN/4503 BEN/4503 Thèse Bibliothèque principale Thèses Disponible
Titre : Activité Killer chez des levures isolées des sols du Nord-Est Algérien : Purification, caractérisation et effet sur les souches de levures indésirables. Type de document : texte imprimé Auteurs : Fatima-Zohra-Kenza Labbani, Auteur ; Zahia Meraihi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2015 Importance : 103 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : levures isolement Pichia kluyveri toxine killer biocontrôle levures indésirables Yeast isolation killer toxin biocontrol undesirable yeasts خمائر عزل سم قاتل مكافحة حيوية خمائر غير مرغوب فيها Index. décimale : 570 Sciences de la vie. Biologie Résumé : The general purpose of this thesis is to search for killer activity in yeast strains isolated from agricultural and forest soil in the region of Constantine for biotechnological applications.The isolation of yeasts allows to list 15 strains. The molecular taxonomy based on the sequences of D1/D2 domain of the 26S ribosomal ARN gene groups these isolates into 6 different species: Cryptococcus aerius, Hanseniaspora opuntiae, Hanseniaspora uvarum, Pichia kluyveri, Meyerozyma guilliermondii and Saccharomyces cerevisiae. Morphological, physiological and biochemical characteristics of the strains are in agreement with those cited in
literature. Preliminary testing for killer activity reveals that only the strain L5 is able to produce killer activity. The killer strain is belonging to the species P. kluyveri. The crude killer protein produced by the strain P. kluyveri reveals that the toxin is active against food and beverage spoilage yeast strains belonging to the genera: Dekkera, Kluyveromyces, Pichia, Saccharomyces, Torulaspora, Wickerhamomyces and Zygosaccharomyces. The highest killer activity is obtained against the strain Dekkera bruxellensis DBVPG 6706. Both the heat shock (30 min at 100°C) and the enzymatic treatment with pronase demonstrate the complete loss of the initial killer activity of the toxin, and hence its proteinaceous nature is apparently confirmed. The active protein migrates as a single band in SDS-PAGE and has a molecular mass of 54 kDa. The purified killer toxin has an optimal pH between 4.0-4.5, while the optimal of temperature is 25°C.The determination of minimum inhibitory concentrations (MICs) shows that the purified killer protein has a high in vitro activity against D.bruxellensis (MICs from 64 000- to 256 000-fold lower than that exhibited by potassium metabisulphite) and Saccharomyces cerevisiae (MICs from 32 000- to 64 000-fold lower than potassium sorbate).
No in vitro synergistic interactions (calculated by FIC index -Σ FIC) are observed when killer protein is used in combination with the active compounds : potassium metabisulphite, potassium sorbate, or ethanol. The killer protein exhibited a dose–response effect against D.
bruxellensis and S. cerevisiae in a soft drink « Campari MIXX » (Milan, Italy) and pear juice « Santal » (Parma, Italy), respectively. The results of the present study suggest that the killer protein could be proposed as a novel food-grade compound useful for the control of food and beverage spoilage yeasts. Additionally, the purified killer protein maintains its killing action at
least for 3 days against S. cerevisiae DBVPG 6500 inoculated in pear juice and for 10 days against D. bruxellensis DBVPG 6706 in soft drink. The results of the present study suggest that the killer protein produced by the strain P. kluyveri (L5) could be proposed as a novel natural agent for the biocontrole of beverage spoilage yeasts.
Diplôme : Doctorat En ligne : ../theses/biologie/LAB6794.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10026 Activité Killer chez des levures isolées des sols du Nord-Est Algérien : Purification, caractérisation et effet sur les souches de levures indésirables. [texte imprimé] / Fatima-Zohra-Kenza Labbani, Auteur ; Zahia Meraihi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2015 . - 103 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : levures isolement Pichia kluyveri toxine killer biocontrôle levures indésirables Yeast isolation killer toxin biocontrol undesirable yeasts خمائر عزل سم قاتل مكافحة حيوية خمائر غير مرغوب فيها Index. décimale : 570 Sciences de la vie. Biologie Résumé : The general purpose of this thesis is to search for killer activity in yeast strains isolated from agricultural and forest soil in the region of Constantine for biotechnological applications.The isolation of yeasts allows to list 15 strains. The molecular taxonomy based on the sequences of D1/D2 domain of the 26S ribosomal ARN gene groups these isolates into 6 different species: Cryptococcus aerius, Hanseniaspora opuntiae, Hanseniaspora uvarum, Pichia kluyveri, Meyerozyma guilliermondii and Saccharomyces cerevisiae. Morphological, physiological and biochemical characteristics of the strains are in agreement with those cited in
literature. Preliminary testing for killer activity reveals that only the strain L5 is able to produce killer activity. The killer strain is belonging to the species P. kluyveri. The crude killer protein produced by the strain P. kluyveri reveals that the toxin is active against food and beverage spoilage yeast strains belonging to the genera: Dekkera, Kluyveromyces, Pichia, Saccharomyces, Torulaspora, Wickerhamomyces and Zygosaccharomyces. The highest killer activity is obtained against the strain Dekkera bruxellensis DBVPG 6706. Both the heat shock (30 min at 100°C) and the enzymatic treatment with pronase demonstrate the complete loss of the initial killer activity of the toxin, and hence its proteinaceous nature is apparently confirmed. The active protein migrates as a single band in SDS-PAGE and has a molecular mass of 54 kDa. The purified killer toxin has an optimal pH between 4.0-4.5, while the optimal of temperature is 25°C.The determination of minimum inhibitory concentrations (MICs) shows that the purified killer protein has a high in vitro activity against D.bruxellensis (MICs from 64 000- to 256 000-fold lower than that exhibited by potassium metabisulphite) and Saccharomyces cerevisiae (MICs from 32 000- to 64 000-fold lower than potassium sorbate).
No in vitro synergistic interactions (calculated by FIC index -Σ FIC) are observed when killer protein is used in combination with the active compounds : potassium metabisulphite, potassium sorbate, or ethanol. The killer protein exhibited a dose–response effect against D.
bruxellensis and S. cerevisiae in a soft drink « Campari MIXX » (Milan, Italy) and pear juice « Santal » (Parma, Italy), respectively. The results of the present study suggest that the killer protein could be proposed as a novel food-grade compound useful for the control of food and beverage spoilage yeasts. Additionally, the purified killer protein maintains its killing action at
least for 3 days against S. cerevisiae DBVPG 6500 inoculated in pear juice and for 10 days against D. bruxellensis DBVPG 6706 in soft drink. The results of the present study suggest that the killer protein produced by the strain P. kluyveri (L5) could be proposed as a novel natural agent for the biocontrole of beverage spoilage yeasts.
Diplôme : Doctorat En ligne : ../theses/biologie/LAB6794.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10026 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAB/6794 LAB/6794 Thèse Bibliothèque principale Thèses Disponible 
Titre : Cellulases de souches fongiques issues du sol d'un milieu extreme (sol proche de sources thermales) : Sélection des souches et étude des caractéristiques des enzymes. Type de document : texte imprimé Auteurs : Hind Leghlimi, Auteur ; Zahia Meraihi, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2013 Autre Editeur : Ardenne : Université de Reims Importance : 135 f. Format : 30 cm Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Moisissures isolement écosystèmes extrêmes cellulase ß-glucosidase thermostabilité Fungi isolation extreme ecosystems thermostability فطریات خیطیة عزل مناطق قاسیة إنزیم ال beta "glucosidase "
الثبات الحراريIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Filamentous fungi found in soils surrounding the hydrothermal stations of regions in the east of Algeria: Guelma (Hammam Debagh) and Mila (Hammam Grouz-Atmania, Hammam Safsaf-Teleghma), are screened for the presence of cellulase activity. 88 fungal strains were
isolated and identified as belonging to six genera: Aspergillus, Alternaria, Emericella, Fusarium, Penicillium and Trichoderma. Their selection (filter paper test and test of perforated plates) shows that only the strain J2 has a significant cellulolytic activity. This
isolate belongs to the species of Trichoderma longibrachiatum Rifai. At 35°C, our isolate shows equivalent activities filter paper and endoglucanase than T. reesei. On the other hand the β-glucosidase activity of our isolate was until twice more important than T. reseei one.
With an inoculum size of 106 spores/ml the strain produces the maximum enzyme activities.
A good yield of enzyme is obtained with spores aged of six days. The strain D allowed from subcloning cultivated in 4 liters fermenter on Mandels medium with cellulose Avicel (1%) produces maximum activities of filter paper (1.88UI/ml), endoglucanase (11.22UI/ml) and β-
glucosidase (0.66UI/ml) after 128 hours, 144 hours and 120 hours, respectively. The optimum temperatures were 55°C and 60°C for endoglucanase and FPA, respectively. The endoglucanase was optimally active at pH 4.0, and the FPA was optimal at pH 4.0 and 5.0.
The endoglucanase was thermostable at 70°C after 5 hours incubation, preserved 80% of the original activity. The half-life of the filter paper activity appeared to be 3 hours at 60°C.
These activities were stable at 50°C after 5 hours incubation in a pH range of 3.0 to 6.0 and 4.0 to 6.0, respectively. The EDTA (5mM) causes a significant diminution of the cellulasic activity, while the β-mercaptoethanol (5mM) leading to their activation. Divalent cations calcium (Ca2+) and zinc (Zn2+) cause an increase and improvement of enzymes activities in the presence of EDTA (5mM). The crude enzyme extract is able to hydrolyse insoluble cellulosic substrates. This enzyme can be classified as an endo and exo type of the cellulase.
These results suggested that the no-mutated strain Trichoderma longibrachiatum should be an attractive producer for cellulases production.Diplôme : Doctorat en sciences En ligne : ..//theses/biologie/LEG6420.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9439 Cellulases de souches fongiques issues du sol d'un milieu extreme (sol proche de sources thermales) : Sélection des souches et étude des caractéristiques des enzymes. [texte imprimé] / Hind Leghlimi, Auteur ; Zahia Meraihi, Directeur de thèse . - constantine [Algérie] : Université Constantine 1 : Ardenne : Université de Reims, 2013 . - 135 f. ; 30 cm.
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Moisissures isolement écosystèmes extrêmes cellulase ß-glucosidase thermostabilité Fungi isolation extreme ecosystems thermostability فطریات خیطیة عزل مناطق قاسیة إنزیم ال beta "glucosidase "
الثبات الحراريIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Filamentous fungi found in soils surrounding the hydrothermal stations of regions in the east of Algeria: Guelma (Hammam Debagh) and Mila (Hammam Grouz-Atmania, Hammam Safsaf-Teleghma), are screened for the presence of cellulase activity. 88 fungal strains were
isolated and identified as belonging to six genera: Aspergillus, Alternaria, Emericella, Fusarium, Penicillium and Trichoderma. Their selection (filter paper test and test of perforated plates) shows that only the strain J2 has a significant cellulolytic activity. This
isolate belongs to the species of Trichoderma longibrachiatum Rifai. At 35°C, our isolate shows equivalent activities filter paper and endoglucanase than T. reesei. On the other hand the β-glucosidase activity of our isolate was until twice more important than T. reseei one.
With an inoculum size of 106 spores/ml the strain produces the maximum enzyme activities.
A good yield of enzyme is obtained with spores aged of six days. The strain D allowed from subcloning cultivated in 4 liters fermenter on Mandels medium with cellulose Avicel (1%) produces maximum activities of filter paper (1.88UI/ml), endoglucanase (11.22UI/ml) and β-
glucosidase (0.66UI/ml) after 128 hours, 144 hours and 120 hours, respectively. The optimum temperatures were 55°C and 60°C for endoglucanase and FPA, respectively. The endoglucanase was optimally active at pH 4.0, and the FPA was optimal at pH 4.0 and 5.0.
The endoglucanase was thermostable at 70°C after 5 hours incubation, preserved 80% of the original activity. The half-life of the filter paper activity appeared to be 3 hours at 60°C.
These activities were stable at 50°C after 5 hours incubation in a pH range of 3.0 to 6.0 and 4.0 to 6.0, respectively. The EDTA (5mM) causes a significant diminution of the cellulasic activity, while the β-mercaptoethanol (5mM) leading to their activation. Divalent cations calcium (Ca2+) and zinc (Zn2+) cause an increase and improvement of enzymes activities in the presence of EDTA (5mM). The crude enzyme extract is able to hydrolyse insoluble cellulosic substrates. This enzyme can be classified as an endo and exo type of the cellulase.
These results suggested that the no-mutated strain Trichoderma longibrachiatum should be an attractive producer for cellulases production.Diplôme : Doctorat en sciences En ligne : ..//theses/biologie/LEG6420.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9439 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LEG/6420 LEG/6420 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte integraleAdobe Acrobat PDF
Titre : Modélisation d'un plancher chauffant cas de la région de Hassi Messaoud Auteurs : Hassina Bourefis ; Univ. de Constantine, Éditeur scientifique ; Abdenacer Kaabi, Directeur de thèse Année de publication : 2000 Importance : 52 f. Note générale : 1 disponible à la salle de recherche
2 disponibles au magasin de la biblioèque centraleLangues : Français (fre) Catégories : Français - Anglais
Génie ClimatiqueTags : Modélisation Plancher chauffant isolation Epaisseur optimisée Index. décimale : 626 Génie Climatique Permalink : index.php?lvl=notice_display&id=22 Modélisation d'un plancher chauffant cas de la région de Hassi Messaoud [] / Hassina Bourefis ; Univ. de Constantine, Éditeur scientifique ; Abdenacer Kaabi, Directeur de thèse . - 2000 . - 52 f.
1 disponible à la salle de recherche
2 disponibles au magasin de la biblioèque centrale
Langues : Français (fre)
Catégories : Français - Anglais
Génie ClimatiqueTags : Modélisation Plancher chauffant isolation Epaisseur optimisée Index. décimale : 626 Génie Climatique Permalink : index.php?lvl=notice_display&id=22 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/3488 BOU/3488 Thèse Bibliothèque principale Thèses Disponible
