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Auteur F. Smati |
Documents disponibles écrits par cet auteur (4)
Affiner la recherche Interroger des sources externesContamination du poulet de chair par les salmonelles non typhiques en élevages et abbatoirs de la wilaya de Constantine / Rachid ELgroud
Titre : Contamination du poulet de chair par les salmonelles non typhiques en élevages et abbatoirs de la wilaya de Constantine : caractérisations phénotypiques et génotypiques par ERIC-PCR,IS-PCR et PFGE Type de document : texte imprimé Auteurs : Rachid ELgroud, Auteur ; F. Smati, Directeur de thèse ; Y. Millemann, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2009 Importance : 116 f. Format : 30 cm. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C
01 CD 02 Disponibles au magazinLangues : Français (fre) Catégories : Français - Anglais
Sciences VétérinairesTags : Facteurs de risque Sérotypie Salmonelles Viande du poulet Résistance au antibiotiques eric-PCR is-PCR PFGE
Salmonella Broiler Chicken Prevalence Serotype Antibiotic-resistance Risk
factors دجاج اللحوم النوع السيرولوجي مقاومة المضادات الحيوية عوامل الخطرIndex. décimale : 636 Sciences Vétérinaires Résumé : The present study provides the first data about the prevalence of Salmonella contamination of broilers and slaughterhouses in the region of Constantine, Algeria. The serotypes and antibiotic resistance patterns of the isolates were determined, and risk factors contributing to the contamination were evaluated.
A total number of 2490 samples, 1800 originating from 30 broiler farms and 690 from 15 slaughterhouses, were taken during 2 periods: October 2005-June 2006 and September 2006- March 2007. Salmonella contamination concerned 37 % of the broiler farms and 73 % of the slaughterhouses.
Among the 55 isolates recovered, ten different serotypes were identified. The most frequently recovered serotypes in both slaughterhouses and breeder farms were S. Hadar (36.4%, n=20), S.
Virchow (16.4%, n=9), S. Infantis (10.9%, n=6), and S. Albany (10.9%, n=6). S. Carnac (7.3%,n=4). Isolates belonging to S. Heidelberg (1.8%, n=1), and S. Rissen (1.8%, n=1) were found only in farms, while those belonging to S. Typhimurium (9.1%, n=5), S. Enteritidis (3.6%, n=2), and S. Montevideo (1.8%, n=1) were recovered only from slaughterhouses. Thirty nine isolates (79.9 %) were resistant to at least one antibiotic and 50.9 % were multi-resistant, i.e. resistant to two or more antibiotic molecules. Among the 55 isolates, 56.4% (n=31) were resistant to streptomycin, 34.5% (n=19) to tetracyclines, 27.2 % (n=15) to nalidixic acid, 12.7% (n=7) to ofloxacin and 1.8% (n=1) to enrofloxacin. Finally, 7 distinct antibiotic resistance profiles were identified.
In parallel, four risk factors were found that were significantly associated with Salmonella contamination. These risk factors highlight the hazards of the broiler channel, particularly linked to poor technical and hygiene practices.
On the another side, the genotypic characterization of the stocks gave us 16 profiles per EricPCR, 20 profiles by is-PCR and 34 profiles by PFGE. The 3 methods, and particularly the PFGE, showed the clonality of some sérotypes, confirming the diffusion and the persistence of the same clone through the field, and in our area, but also the polymorphism of others sérotypes indicating the diversity of the potential tanks in nontyphoidal salmonella.
The comparison of our PFGE profiles to those of the bank of data of the AFSSA, Maisons Alfort, France, informs us about the diffusion, sometimes international, of some sérotypes, facilitated by the increasing levels of trade, at the eve of the accession of Algeria to the World Trade Organization.Diplôme : Doctorat en sciences En ligne : ../theses/veterinaire/ELG5288.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1868 Contamination du poulet de chair par les salmonelles non typhiques en élevages et abbatoirs de la wilaya de Constantine : caractérisations phénotypiques et génotypiques par ERIC-PCR,IS-PCR et PFGE [texte imprimé] / Rachid ELgroud, Auteur ; F. Smati, Directeur de thèse ; Y. Millemann, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2009 . - 116 f. ; 30 cm.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C
01 CD 02 Disponibles au magazin
Langues : Français (fre)
Catégories : Français - Anglais
Sciences VétérinairesTags : Facteurs de risque Sérotypie Salmonelles Viande du poulet Résistance au antibiotiques eric-PCR is-PCR PFGE
Salmonella Broiler Chicken Prevalence Serotype Antibiotic-resistance Risk
factors دجاج اللحوم النوع السيرولوجي مقاومة المضادات الحيوية عوامل الخطرIndex. décimale : 636 Sciences Vétérinaires Résumé : The present study provides the first data about the prevalence of Salmonella contamination of broilers and slaughterhouses in the region of Constantine, Algeria. The serotypes and antibiotic resistance patterns of the isolates were determined, and risk factors contributing to the contamination were evaluated.
A total number of 2490 samples, 1800 originating from 30 broiler farms and 690 from 15 slaughterhouses, were taken during 2 periods: October 2005-June 2006 and September 2006- March 2007. Salmonella contamination concerned 37 % of the broiler farms and 73 % of the slaughterhouses.
Among the 55 isolates recovered, ten different serotypes were identified. The most frequently recovered serotypes in both slaughterhouses and breeder farms were S. Hadar (36.4%, n=20), S.
Virchow (16.4%, n=9), S. Infantis (10.9%, n=6), and S. Albany (10.9%, n=6). S. Carnac (7.3%,n=4). Isolates belonging to S. Heidelberg (1.8%, n=1), and S. Rissen (1.8%, n=1) were found only in farms, while those belonging to S. Typhimurium (9.1%, n=5), S. Enteritidis (3.6%, n=2), and S. Montevideo (1.8%, n=1) were recovered only from slaughterhouses. Thirty nine isolates (79.9 %) were resistant to at least one antibiotic and 50.9 % were multi-resistant, i.e. resistant to two or more antibiotic molecules. Among the 55 isolates, 56.4% (n=31) were resistant to streptomycin, 34.5% (n=19) to tetracyclines, 27.2 % (n=15) to nalidixic acid, 12.7% (n=7) to ofloxacin and 1.8% (n=1) to enrofloxacin. Finally, 7 distinct antibiotic resistance profiles were identified.
In parallel, four risk factors were found that were significantly associated with Salmonella contamination. These risk factors highlight the hazards of the broiler channel, particularly linked to poor technical and hygiene practices.
On the another side, the genotypic characterization of the stocks gave us 16 profiles per EricPCR, 20 profiles by is-PCR and 34 profiles by PFGE. The 3 methods, and particularly the PFGE, showed the clonality of some sérotypes, confirming the diffusion and the persistence of the same clone through the field, and in our area, but also the polymorphism of others sérotypes indicating the diversity of the potential tanks in nontyphoidal salmonella.
The comparison of our PFGE profiles to those of the bank of data of the AFSSA, Maisons Alfort, France, informs us about the diffusion, sometimes international, of some sérotypes, facilitated by the increasing levels of trade, at the eve of the accession of Algeria to the World Trade Organization.Diplôme : Doctorat en sciences En ligne : ../theses/veterinaire/ELG5288.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1868 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ELG/5288 ELG/5288 Thèse Bibliothèque principale Thèses Disponible
Titre : Contribution à l'étude des infections intramammaires de la vache laitiere dans l'Est algeriens Type de document : texte imprimé Auteurs : Omar Bouaziz, Auteur ; F. Smati, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2005 Importance : 235 f. Format : 30 cm. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la bibliothèque centrale
01 CDLangues : Français (fre) Catégories : Français - Anglais
Sciences VétérinairesTags : Bactériologie Vache laitière Antibiorésistance Mammite clinique Mammite subclinique CMT Résidus d'antibiotiques Traitement au tarissement dairy cattle clinical mastitis subclinical mastitis bacteriolgy Antibiotic residues antibiotic resistance dry cow therapy Index. décimale : 636 Sciences Vétérinaires Résumé : The control of the infections intramammaires represents a paramount stake for the dairy stockbreeders. To fight against this pathology passes by a knowledge of the bacteria in causes and their epidemiology.
The present study related on the one hand, on the evaluation of the prévalence of the germs responsible for the clinical mastitis and the study of some aspects of their epidemiology in 35 dairy herds and on the other hand, to evaluate the prévalence and the etiology of the subclinic mastitis in the cows at the end of the lactation in four dairy herds. The performance of the CMT to identify subclincal mastitis at the end of the lactation was evaluated. The in vitro antimicrobial susceptibility of bacteria isolated from cow milk samples was tested. Treatment of dairy cows at drying off has been practiced.
Results obtained showed :
A strong prévalence (32,6%) of the clinical cows with clinical mastitis. The bacteriological analysis of 252 milk samples milk showed : 28,4% of Staphylococcus aureus, 21,6% of Eschérichia coli, 13,7% of Streptococcus agalactiae. The coagulase negative staphylococci with a frequency of 10,8% seem increasingly accused in the clinical mastitis.
The prévalence of the cows with subclnical mastitis at the end of the lactation is 73,6% and the quarters 40,3%. The frequency of the germs responsible for subclnical mastitis was estimated on 121 cows at the end of the lactation. Out of he 464 milk samples, 59,7% were bacteriological negative. The germs observed most often are :
Staphylococcus aureus (30,9%), coagulase negative staphylococci (25,9%), Streptococcus agalactiae (23,2%) and Eschérichia coli (15,9%).
Staphylococcus aureus is the predominant bacterial pathogen in subclinical and clinical mastitis. Environmental pathogens are not to neglect in the cause of the clinical mastits.
Moreover, this study revealed the presence of antibiotic residues in 17,1% of the 35 milk samples analyzed.
The in vitro antimicrobial susceptibility of bacteria isolated from cow milk samples showed high resistance to penicillin, ampicillin, oxacillin, tetracyclin, streptomycin and erythromycin.
During the dry period, the treatment eliminates 91% of pathogenic organism. The rate of new infections prevented is 88%.Diplôme : Doctorat En ligne : ../theses/veterinaire/BOU4260.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1887 Contribution à l'étude des infections intramammaires de la vache laitiere dans l'Est algeriens [texte imprimé] / Omar Bouaziz, Auteur ; F. Smati, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2005 . - 235 f. ; 30 cm.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la bibliothèque centrale
01 CD
Langues : Français (fre)
Catégories : Français - Anglais
Sciences VétérinairesTags : Bactériologie Vache laitière Antibiorésistance Mammite clinique Mammite subclinique CMT Résidus d'antibiotiques Traitement au tarissement dairy cattle clinical mastitis subclinical mastitis bacteriolgy Antibiotic residues antibiotic resistance dry cow therapy Index. décimale : 636 Sciences Vétérinaires Résumé : The control of the infections intramammaires represents a paramount stake for the dairy stockbreeders. To fight against this pathology passes by a knowledge of the bacteria in causes and their epidemiology.
The present study related on the one hand, on the evaluation of the prévalence of the germs responsible for the clinical mastitis and the study of some aspects of their epidemiology in 35 dairy herds and on the other hand, to evaluate the prévalence and the etiology of the subclinic mastitis in the cows at the end of the lactation in four dairy herds. The performance of the CMT to identify subclincal mastitis at the end of the lactation was evaluated. The in vitro antimicrobial susceptibility of bacteria isolated from cow milk samples was tested. Treatment of dairy cows at drying off has been practiced.
Results obtained showed :
A strong prévalence (32,6%) of the clinical cows with clinical mastitis. The bacteriological analysis of 252 milk samples milk showed : 28,4% of Staphylococcus aureus, 21,6% of Eschérichia coli, 13,7% of Streptococcus agalactiae. The coagulase negative staphylococci with a frequency of 10,8% seem increasingly accused in the clinical mastitis.
The prévalence of the cows with subclnical mastitis at the end of the lactation is 73,6% and the quarters 40,3%. The frequency of the germs responsible for subclnical mastitis was estimated on 121 cows at the end of the lactation. Out of he 464 milk samples, 59,7% were bacteriological negative. The germs observed most often are :
Staphylococcus aureus (30,9%), coagulase negative staphylococci (25,9%), Streptococcus agalactiae (23,2%) and Eschérichia coli (15,9%).
Staphylococcus aureus is the predominant bacterial pathogen in subclinical and clinical mastitis. Environmental pathogens are not to neglect in the cause of the clinical mastits.
Moreover, this study revealed the presence of antibiotic residues in 17,1% of the 35 milk samples analyzed.
The in vitro antimicrobial susceptibility of bacteria isolated from cow milk samples showed high resistance to penicillin, ampicillin, oxacillin, tetracyclin, streptomycin and erythromycin.
During the dry period, the treatment eliminates 91% of pathogenic organism. The rate of new infections prevented is 88%.Diplôme : Doctorat En ligne : ../theses/veterinaire/BOU4260.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1887 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/4260 BOU/4260 Thèse Bibliothèque principale Thèses Disponible
Titre : Détermination du microbisme en élevage avicole Type de document : texte imprimé Auteurs : Amir Agabou, Auteur ; F. Smati, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2006 Importance : 244 f. Format : 30 cm. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
Sciences VétérinairesTags : Désinfection Contamination bactérienne Air Surfaces Nettoyage Sécurité sanitaire Bacterial contamination cleaning and disinfection biosecurity تلوث بكثيري هواء سطوح تنظيف و تطهير حماية صحية Index. décimale : 636 Sciences Vétérinaires Résumé : The study is realised in 08 avian production unities (05 broiler houses, 01 breeders house
and 02 hatcheries) in the Wilaya of Constantine. The aim was to determine the microbial
contamination level (specially bacterial contamination : total flora, staphylococcus,
enterobacteria and salmonella) of the air and the surfaces of each unity before and after cleaning
and disinfection and establishing a critical analysis of the different biosecurity measures taken in
them. A total of 1194 bacterial enumerations and more than 340 searches for salmonella have
been realised.Diplôme : Magistère En ligne : ../theses/veterinaire/AGA4692.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1877 Détermination du microbisme en élevage avicole [texte imprimé] / Amir Agabou, Auteur ; F. Smati, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2006 . - 244 f. ; 30 cm.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
Sciences VétérinairesTags : Désinfection Contamination bactérienne Air Surfaces Nettoyage Sécurité sanitaire Bacterial contamination cleaning and disinfection biosecurity تلوث بكثيري هواء سطوح تنظيف و تطهير حماية صحية Index. décimale : 636 Sciences Vétérinaires Résumé : The study is realised in 08 avian production unities (05 broiler houses, 01 breeders house
and 02 hatcheries) in the Wilaya of Constantine. The aim was to determine the microbial
contamination level (specially bacterial contamination : total flora, staphylococcus,
enterobacteria and salmonella) of the air and the surfaces of each unity before and after cleaning
and disinfection and establishing a critical analysis of the different biosecurity measures taken in
them. A total of 1194 bacterial enumerations and more than 340 searches for salmonella have
been realised.Diplôme : Magistère En ligne : ../theses/veterinaire/AGA4692.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1877 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AGA/4692 AGA/4692 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude épidémiologique des mycoplasmes des petits ruminants : Application de l'immunoblot à l'étude des sérums de moutons infectés expérimentalement par Mycoplasmaagalactiae. Type de document : texte imprimé Auteurs : Rachid Kabouia, Auteur ; F. Smati, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2005 Importance : 168 f. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la bibliothèque centrale
01 CDLangues : Français (fre) Catégories : Français - Anglais
Sciences VétérinairesTags : Ovins Mycoplasmes Immunoblot Anticorps Antigène majeur Index. décimale : 636 Sciences Vétérinaires Résumé : An epidemiologic study on mycoplasmas of sheep, was conducted for the first time in
Algeria
M.agalactiae , M.capricolum, M.ovipneumoniae and M.arginini were found in animals
with symptoms and lesions respiratory. A serological investigation by F.C. and
E.L.I.S.A. revealed the presence of antibodies against mycoplasma.
Furthermore, this strains were identified by biochemical test (arginin hydrolysis,
tetrazolium reduction, film and spots and phosphatase) and serologic(inhibition of
growth in antiserums presence) .
The mycoplasma are the origine of great pathology at the ruminants. To better elucidate
the mecanism of persistency of these bacterial in the animal organism, two experiments
had been realised with P89 strains (M.agalactiae ) and differents doses. During the two
experiments, specific serum antibodies were detected by western blot, determining a
few immunodominant antigen recognised by the immunserums wich will survive for the
further studies in the approach vaccination.Diplôme : Doctorat En ligne : ../theses/veterinaire/KAB4259.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1882 Etude épidémiologique des mycoplasmes des petits ruminants : Application de l'immunoblot à l'étude des sérums de moutons infectés expérimentalement par Mycoplasmaagalactiae. [texte imprimé] / Rachid Kabouia, Auteur ; F. Smati, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2005 . - 168 f.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la bibliothèque centrale
01 CD
Langues : Français (fre)
Catégories : Français - Anglais
Sciences VétérinairesTags : Ovins Mycoplasmes Immunoblot Anticorps Antigène majeur Index. décimale : 636 Sciences Vétérinaires Résumé : An epidemiologic study on mycoplasmas of sheep, was conducted for the first time in
Algeria
M.agalactiae , M.capricolum, M.ovipneumoniae and M.arginini were found in animals
with symptoms and lesions respiratory. A serological investigation by F.C. and
E.L.I.S.A. revealed the presence of antibodies against mycoplasma.
Furthermore, this strains were identified by biochemical test (arginin hydrolysis,
tetrazolium reduction, film and spots and phosphatase) and serologic(inhibition of
growth in antiserums presence) .
The mycoplasma are the origine of great pathology at the ruminants. To better elucidate
the mecanism of persistency of these bacterial in the animal organism, two experiments
had been realised with P89 strains (M.agalactiae ) and differents doses. During the two
experiments, specific serum antibodies were detected by western blot, determining a
few immunodominant antigen recognised by the immunserums wich will survive for the
further studies in the approach vaccination.Diplôme : Doctorat En ligne : ../theses/veterinaire/KAB4259.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1882 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KAB/4259 KAB/4259 Thèse Bibliothèque principale Thèses Disponible
