| Titre : |
Etude phytochimique et biologique de deux plantes médicinales Algériennes. |
| Type de document : |
texte imprimé |
| Auteurs : |
Houria Bechlem ép Boucenna, Auteur ; Mohamed Bouheroum, Directeur de thèse |
| Editeur : |
جامعة الإخوة منتوري قسنطينة |
| Année de publication : |
2018 |
| Importance : |
226 f. |
| Format : |
30 cm. |
| Note générale : |
2 copies imprimées disponibles |
| Langues : |
Français (fre) |
| Catégories : |
Français - Anglais
Chimie
|
| Tags : |
Caryophyllaceae Lamiaceae Gymnocarpos decander Mentha pulegium saponines flavonoïdes acides phénoliques RMN 1D et 2D activités antiprolifératives saponins flavonoids phenolic acids NMR 1D and 2D antiproliferative activity الدراسة الفیتو كیمیائیة عملیة الاستخلاص الفصل الكروماتوغرافي الأیض Mentha pulegiumالقرنقلیة العائلة الشفویة الثانوي العائلة |
| Index. décimale : |
540 Chimie et sciences connexes |
| Résumé : |
The present work deals with the phytochemical study and the evaluation of the biological activity of two plants belonging to the Algerian flora Gymnocarpos decander (Caryophyllaceae) and Mentha pulegium (Lamiaceae).
The Extraction process has been done on the aerial parts of the two species previously mentioned using organic solvents with different methods followed by chromatographic separations (CC, TLC and HPLC), the outcomes of the said extraction gave 19 products of which 5 are new (2 saponins and 3 flavonol glycosides) which have been isolated from nbutanol phase of the hydroalcoholic extract of Gymnocarpos decander, and three known
compounds isolated from the ethyl acetate extract. The remaining 11 products isolated from Mentha pulegium are known flavonoids and phenolic acids.
Structural elucidation of the isolated compounds was performed by high resolution mass spectra (HRESI-MS), 1D NMR (1H, 13C) and 2D NMR (COSY, HSQC, HMBC) and comparing them with data from the literature.
The antiproliferative activity research tests of the five new products isolated from the n-butanol extract of G. decander showed that both saponins are active against Hela cell line and the results have been very promising and hence incite to effect the vivo studies to confirm its pharmacological properties.
Quantitative estimation for total polyphenols of the hydroalcoholic extract (aerial parts) of M. pulegium has shown clearly that this extract has a high content of polyphenols.
Evaluation of the biological activity carried out on this extract showed that this species had a good scavenging effect against the DPPH radical.
On the chemotaxonomic level, the results show a rich and varied composition of secondary metabolites which is matching the plants of the same family characteristics. In fact, for the Caryophyllaceae family, saponins are considered as chemotaxonomic markers. As for the Lamiaceae family, its reputation for flavonoid accumulation is well known.
|
| Diplôme : |
Doctorat |
| En ligne : |
../theses/chimie/BEC7211.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=10780 |
Etude phytochimique et biologique de deux plantes médicinales Algériennes. [texte imprimé] / Houria Bechlem ép Boucenna, Auteur ; Mohamed Bouheroum, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 226 f. ; 30 cm. 2 copies imprimées disponibles Langues : Français ( fre)
| Catégories : |
Français - Anglais
Chimie
|
| Tags : |
Caryophyllaceae Lamiaceae Gymnocarpos decander Mentha pulegium saponines flavonoïdes acides phénoliques RMN 1D et 2D activités antiprolifératives saponins flavonoids phenolic acids NMR 1D and 2D antiproliferative activity الدراسة الفیتو كیمیائیة عملیة الاستخلاص الفصل الكروماتوغرافي الأیض Mentha pulegiumالقرنقلیة العائلة الشفویة الثانوي العائلة |
| Index. décimale : |
540 Chimie et sciences connexes |
| Résumé : |
The present work deals with the phytochemical study and the evaluation of the biological activity of two plants belonging to the Algerian flora Gymnocarpos decander (Caryophyllaceae) and Mentha pulegium (Lamiaceae).
The Extraction process has been done on the aerial parts of the two species previously mentioned using organic solvents with different methods followed by chromatographic separations (CC, TLC and HPLC), the outcomes of the said extraction gave 19 products of which 5 are new (2 saponins and 3 flavonol glycosides) which have been isolated from nbutanol phase of the hydroalcoholic extract of Gymnocarpos decander, and three known
compounds isolated from the ethyl acetate extract. The remaining 11 products isolated from Mentha pulegium are known flavonoids and phenolic acids.
Structural elucidation of the isolated compounds was performed by high resolution mass spectra (HRESI-MS), 1D NMR (1H, 13C) and 2D NMR (COSY, HSQC, HMBC) and comparing them with data from the literature.
The antiproliferative activity research tests of the five new products isolated from the n-butanol extract of G. decander showed that both saponins are active against Hela cell line and the results have been very promising and hence incite to effect the vivo studies to confirm its pharmacological properties.
Quantitative estimation for total polyphenols of the hydroalcoholic extract (aerial parts) of M. pulegium has shown clearly that this extract has a high content of polyphenols.
Evaluation of the biological activity carried out on this extract showed that this species had a good scavenging effect against the DPPH radical.
On the chemotaxonomic level, the results show a rich and varied composition of secondary metabolites which is matching the plants of the same family characteristics. In fact, for the Caryophyllaceae family, saponins are considered as chemotaxonomic markers. As for the Lamiaceae family, its reputation for flavonoid accumulation is well known.
|
| Diplôme : |
Doctorat |
| En ligne : |
../theses/chimie/BEC7211.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=10780 |
|
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