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Auteur Yacine Benhizia |
Documents disponibles écrits par cet auteur (12)
Affiner la recherche Interroger des sources externesBiodiversité de la flore bactérienne du palmier dattier poussant au sud est Algérien. Effet du climat sur le comportement de cette flore. / Wissame Aimene
Titre : Biodiversité de la flore bactérienne du palmier dattier poussant au sud est Algérien. Effet du climat sur le comportement de cette flore. Type de document : texte imprimé Auteurs : Wissame Aimene, Auteur ; Yacine Benhizia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 343 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Phoenix dactylifera Phyllosphère ADNr 16S diversité bactérienne phyllosphere 16S rDNA bacterial diversity التنوع البكتيريADNr 16S Index. décimale : 570 Sciences de la vie. Biologie Résumé : Bacterial flora of the three parts of the date palm (Phoenix dactylifera L.) (Leaves, fruits and trunk) was isolated and then identified following phenotypic and molecular tests (using 16S rDNA). Evaluation of the bacterial population through the different seasons (in 2012) was carried out to demonstrate the temporal dynamics of the cultivable population.
Statistical analysis revealed significant differences in the distribution of bacterial strains with increase or decrease of bacterial levels during the different seasons.
Morphological characters and simple orientation tests allowed selecting 78 representative isolates from more than 200. The results of phenotypic identification made it possible to classify the majority of isolates at the genus level. As well as, the study of the effect of a biotic factors on the growth of isolates revealed that the tested isolates tolerate and survive under severe a biotic conditions.
According to the partial analysis of the gene coding for 16S rRNA, 2 isolates were identified perfectly with 100% similarities, some isolates were identified between 97% to 99% similarity to the strains conserved in Genbank, while others strains were identified between 78% to 96%. Overall, there were 33 bacterial species belonging to 16 genera. The most common genera in all parts of the tree are Bacillus (36.84%), Pseudemonas and Enterobacter representing 17.1% for each, but however its distribution is heterogeneous. The genus Lysinibacillus is the
third most isolated genus (10.52%). Unlike other genera that remain minority such as Staphylococcus, Serratia, Microbacterium, Kluyvera, Arthrobacter, Stenotrophomonas, Exiguobacterium, Micrococcus, Klebsiella, Massilia, Erwinia, and Brevibacterium.
This work gave for the first time in Algeria a global idea about the diversity of the date palm bacterial flora and highlighted the impact of climate on the dynamics of this flora.
Diplôme : Doctorat En ligne : ../theses/biologie/AIM7296.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10911 Biodiversité de la flore bactérienne du palmier dattier poussant au sud est Algérien. Effet du climat sur le comportement de cette flore. [texte imprimé] / Wissame Aimene, Auteur ; Yacine Benhizia, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 343 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Phoenix dactylifera Phyllosphère ADNr 16S diversité bactérienne phyllosphere 16S rDNA bacterial diversity التنوع البكتيريADNr 16S Index. décimale : 570 Sciences de la vie. Biologie Résumé : Bacterial flora of the three parts of the date palm (Phoenix dactylifera L.) (Leaves, fruits and trunk) was isolated and then identified following phenotypic and molecular tests (using 16S rDNA). Evaluation of the bacterial population through the different seasons (in 2012) was carried out to demonstrate the temporal dynamics of the cultivable population.
Statistical analysis revealed significant differences in the distribution of bacterial strains with increase or decrease of bacterial levels during the different seasons.
Morphological characters and simple orientation tests allowed selecting 78 representative isolates from more than 200. The results of phenotypic identification made it possible to classify the majority of isolates at the genus level. As well as, the study of the effect of a biotic factors on the growth of isolates revealed that the tested isolates tolerate and survive under severe a biotic conditions.
According to the partial analysis of the gene coding for 16S rRNA, 2 isolates were identified perfectly with 100% similarities, some isolates were identified between 97% to 99% similarity to the strains conserved in Genbank, while others strains were identified between 78% to 96%. Overall, there were 33 bacterial species belonging to 16 genera. The most common genera in all parts of the tree are Bacillus (36.84%), Pseudemonas and Enterobacter representing 17.1% for each, but however its distribution is heterogeneous. The genus Lysinibacillus is the
third most isolated genus (10.52%). Unlike other genera that remain minority such as Staphylococcus, Serratia, Microbacterium, Kluyvera, Arthrobacter, Stenotrophomonas, Exiguobacterium, Micrococcus, Klebsiella, Massilia, Erwinia, and Brevibacterium.
This work gave for the first time in Algeria a global idea about the diversity of the date palm bacterial flora and highlighted the impact of climate on the dynamics of this flora.
Diplôme : Doctorat En ligne : ../theses/biologie/AIM7296.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10911 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AIM/7296 AIM/7296 Thèse Bibliothèque principale Thèses Disponible
Titre : Caractérisation des bactéries isolées à partir du genre Trigonella L. (Légumineuses) possant dans différents écosystèmes de l'Est algérien Type de document : texte imprimé Auteurs : Rabah Chabbi, Auteur ; Univ. de Constantine, Éditeur scientifique ; Yacine Benhizia, Directeur de thèse Année de publication : 2009 Importance : 103 f. Note générale : 01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Caractérisation Rhizobium Gammaproteobacteria Symbiote Trrigonella Characterization Symbiots Trogonella L وصف متعايش Index. décimale : 570 Sciences de la vie. Biologie Résumé : This work has been realized in order to evaluation and caracterisation of the
phenotypical diversity that exists within a collection of symbiots isolated from nodosities of
three Fenugreek populations originated form different regions of east Algeria.
The SDS page has revealed a great level of proteic polymorphism between the isolates. This
technique has allowed us to highlight the possible multiple infection among Trigonella L.
All the strains are infective towards Trigonella L with a relative efficiency that is
reflected by a good growing level of the obtained feet.
The fast growth of the strains and the assimilation of the charred substratum have
revealed profiles that are comparable to those of Rhizobium. In contrast, the strains have
manifested, like the Gammaproteobacteria, a great resistance to heavy metals and antibiotics.
A great tolerance to NaCl, pH, and temperature variation has been noticed for the whole
collection.
We have defined, on the base of the Azote fixative potential and the tolerance of
alkalinity, candidates that are efficient enough to be used for inoculation in the field.Diplôme : Magistère En ligne : ../theses/biologie/CHA5602.pdf Permalink : index.php?lvl=notice_display&id=1817 Caractérisation des bactéries isolées à partir du genre Trigonella L. (Légumineuses) possant dans différents écosystèmes de l'Est algérien [texte imprimé] / Rabah Chabbi, Auteur ; Univ. de Constantine, Éditeur scientifique ; Yacine Benhizia, Directeur de thèse . - 2009 . - 103 f.
01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Caractérisation Rhizobium Gammaproteobacteria Symbiote Trrigonella Characterization Symbiots Trogonella L وصف متعايش Index. décimale : 570 Sciences de la vie. Biologie Résumé : This work has been realized in order to evaluation and caracterisation of the
phenotypical diversity that exists within a collection of symbiots isolated from nodosities of
three Fenugreek populations originated form different regions of east Algeria.
The SDS page has revealed a great level of proteic polymorphism between the isolates. This
technique has allowed us to highlight the possible multiple infection among Trigonella L.
All the strains are infective towards Trigonella L with a relative efficiency that is
reflected by a good growing level of the obtained feet.
The fast growth of the strains and the assimilation of the charred substratum have
revealed profiles that are comparable to those of Rhizobium. In contrast, the strains have
manifested, like the Gammaproteobacteria, a great resistance to heavy metals and antibiotics.
A great tolerance to NaCl, pH, and temperature variation has been noticed for the whole
collection.
We have defined, on the base of the Azote fixative potential and the tolerance of
alkalinity, candidates that are efficient enough to be used for inoculation in the field.Diplôme : Magistère En ligne : ../theses/biologie/CHA5602.pdf Permalink : index.php?lvl=notice_display&id=1817 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité CHA/5602 CHA/5602 Thèse Bibliothèque principale Thèses Disponible
Titre : Caractérisation des bactéries isolées de sols contaminés par les hydrocarbures (zone de Skikda) productrices des biosurfactants Type de document : texte imprimé Auteurs : Ibtissem Guergouri, Auteur ; Univ. de Constantine, Éditeur scientifique ; Yacine Benhizia, Directeur de thèse Année de publication : 2010 Importance : 74 f. Note générale : Magister
01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CDLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Bactéries Hydrocarbures Biosurfactants Emulsion Sols pollués Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Magistère En ligne : ../theses/biologie/GUE5784.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1820 Caractérisation des bactéries isolées de sols contaminés par les hydrocarbures (zone de Skikda) productrices des biosurfactants [texte imprimé] / Ibtissem Guergouri, Auteur ; Univ. de Constantine, Éditeur scientifique ; Yacine Benhizia, Directeur de thèse . - 2010 . - 74 f.
Magister
01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Bactéries Hydrocarbures Biosurfactants Emulsion Sols pollués Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Magistère En ligne : ../theses/biologie/GUE5784.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1820 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité GUE/5784 GUE/5784 Thèse Bibliothèque principale Thèses Disponible
Titre : Caractérisation des isolats bactériens par des techniques phénotyques et électrophorétiques isolés à partir des nodules de queques espèces de légumineuses spontanées de la tribu des Ginesteae (Fabaceae), Type de document : texte imprimé Auteurs : Ahmed Dekak, Auteur ; Yacine Benhizia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 117 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Biologie et Ecologie Végétale: Biotechnologies Végétale Diversité des Rhizobia Legumeuses (Fabaceae) indigènes Caractérisation phénotypique et
polyphasique Approche MCMC 16S rRNA Régions arides et semi-arides Rhizobia diversity Spontaneous legums (Fabaceae) phenotypic and polyphasic
characterization Bayesian MCMC approach 16S RNA, drylands soils البقىليات البرية المناطق الجافة والشبه جافة التنوع البيولوجي للبكتيريا الطريقة
الإحصائية MCMCالتصنيف المظهري والوراثي للبكتيريا((16S rRNIndex. décimale : 540 Chimie et sciences connexes Résumé : Most studies of rhizobia have focused on identification and systematics of symbionts nodulating crop legumes because of their interest in improving yield. However, few studies investigated the endosymbiotic nitrogen-fixing bacteria nodulating wild and spontaneous legume species, in particular in North Africa. This study aims to phenotypically characterize endosymbiotic bacteria isolated from root-nodules of a wild legumes growing in arid and semiarid regions of northeast Algeria. Genista microcephala and Argyrolobium uniflorum by chemotaxonomic, biochemical profiling and symbiotic properties comparing them with reference strains (RS). In addition polyphasic characterization of bacteria isolated from nodules of Genista cinerea. It examines growth variations of endosymbiont isolates under different environmental conditions including severe situations and to determine their taxonomic position based on growth tolerance limits and potential adaptations, using advanced statistical modelling techniques and genomic characterization based on 16S rRNA sequencing. Phenotypic characterization was performed using physiological tests (different pH levels, NaCl concentrations, and tolerance to temperature), nutritional experiments (assimilation of different carbohydrates and amino acids) and biochemical profiling (antibiotic resistance, heavy metals resistance and protein profile). Polyphasic characterization was conducted using physiological tests, nutritional experiments and genomic characterization based on 16S rRNA sequencing. Data were analyzed using Markov Chain Monte Carlo sampler for multivariate generalized linear mixed models (MCMCglmm) to detect growth differences between isolates and RS. Similarities between isolates and RS were assessed using agglomerative hierarchical clustering (AHC), whereas multiple factor analysis (MFA) was performed to understand factors influencing each group of isolates/RS and Phylogenie of isolates was conducted. The results showed that the isolates can grow at pH [4–10], salt concentration [NaCl=0–5%] and temperature up to 45°C. The rhizobia associated with G. microcephala and A. uniflorum were able to produce different hydrolytic enzymes including cellulose, pectinase and urease, with remarkable tolerance to toxic metals such as zinc, lead, copper, and mercury. Numerical analysis of the phenotypic characteristics revealed that the rhizobial isolates formed four main distinct groups showing high levels of similarity with Gammaproteobacteria. Symbiotic and cultural characteristics of G. cinerea isolats revealed the existence of a large physiological diversity among tested bacteria, which showed a broad capability to assimilate different carbonaceous and nitrogenous substances, with consistent and large tolerances to pH [4–10], temperature [4–55°C], and salinity [NaCl=2–10%]. Although, the endosymbiont isolates have broad metabolic diversity, they formed two distinct groups with high level of similarity with RS. Group 1 included fast-growing and salt-tolerant isolates characterized by tolerance to acidity with high growth in alkaline conditions. Group 2 covered slow-growing acid-sensitive isolates that high salinity negatively affected their growth. Results of AHC and MFA evidenced that bacterial diversity of endosymbiont isolates showed high level of similarity with RS, a proof that they are rhizobial strains. In concordance with phylogenitic results isolates are closely related to different rhizobia species The salt tolerant and heavy metals resistance patterns found among the indigenous rhizobial strains associated to G. microcephala and A. uniflorum are reflecting the environmental stresses pressure and make the strains good candidates for plant successful inoculation in arid areas. Our findings indicate that both fast- and slow-growing rhizobia nodulated G. cinerea growing in arid and semiarid regions of northeast Algeria. These rhizobia are poly-extremophiles adapted to diverse environmental stresses and linked to Beta and Gammaprotéobacteria. Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/DEK7408.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11060 Caractérisation des isolats bactériens par des techniques phénotyques et électrophorétiques isolés à partir des nodules de queques espèces de légumineuses spontanées de la tribu des Ginesteae (Fabaceae), [texte imprimé] / Ahmed Dekak, Auteur ; Yacine Benhizia, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 117 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Biologie et Ecologie Végétale: Biotechnologies Végétale Diversité des Rhizobia Legumeuses (Fabaceae) indigènes Caractérisation phénotypique et
polyphasique Approche MCMC 16S rRNA Régions arides et semi-arides Rhizobia diversity Spontaneous legums (Fabaceae) phenotypic and polyphasic
characterization Bayesian MCMC approach 16S RNA, drylands soils البقىليات البرية المناطق الجافة والشبه جافة التنوع البيولوجي للبكتيريا الطريقة
الإحصائية MCMCالتصنيف المظهري والوراثي للبكتيريا((16S rRNIndex. décimale : 540 Chimie et sciences connexes Résumé : Most studies of rhizobia have focused on identification and systematics of symbionts nodulating crop legumes because of their interest in improving yield. However, few studies investigated the endosymbiotic nitrogen-fixing bacteria nodulating wild and spontaneous legume species, in particular in North Africa. This study aims to phenotypically characterize endosymbiotic bacteria isolated from root-nodules of a wild legumes growing in arid and semiarid regions of northeast Algeria. Genista microcephala and Argyrolobium uniflorum by chemotaxonomic, biochemical profiling and symbiotic properties comparing them with reference strains (RS). In addition polyphasic characterization of bacteria isolated from nodules of Genista cinerea. It examines growth variations of endosymbiont isolates under different environmental conditions including severe situations and to determine their taxonomic position based on growth tolerance limits and potential adaptations, using advanced statistical modelling techniques and genomic characterization based on 16S rRNA sequencing. Phenotypic characterization was performed using physiological tests (different pH levels, NaCl concentrations, and tolerance to temperature), nutritional experiments (assimilation of different carbohydrates and amino acids) and biochemical profiling (antibiotic resistance, heavy metals resistance and protein profile). Polyphasic characterization was conducted using physiological tests, nutritional experiments and genomic characterization based on 16S rRNA sequencing. Data were analyzed using Markov Chain Monte Carlo sampler for multivariate generalized linear mixed models (MCMCglmm) to detect growth differences between isolates and RS. Similarities between isolates and RS were assessed using agglomerative hierarchical clustering (AHC), whereas multiple factor analysis (MFA) was performed to understand factors influencing each group of isolates/RS and Phylogenie of isolates was conducted. The results showed that the isolates can grow at pH [4–10], salt concentration [NaCl=0–5%] and temperature up to 45°C. The rhizobia associated with G. microcephala and A. uniflorum were able to produce different hydrolytic enzymes including cellulose, pectinase and urease, with remarkable tolerance to toxic metals such as zinc, lead, copper, and mercury. Numerical analysis of the phenotypic characteristics revealed that the rhizobial isolates formed four main distinct groups showing high levels of similarity with Gammaproteobacteria. Symbiotic and cultural characteristics of G. cinerea isolats revealed the existence of a large physiological diversity among tested bacteria, which showed a broad capability to assimilate different carbonaceous and nitrogenous substances, with consistent and large tolerances to pH [4–10], temperature [4–55°C], and salinity [NaCl=2–10%]. Although, the endosymbiont isolates have broad metabolic diversity, they formed two distinct groups with high level of similarity with RS. Group 1 included fast-growing and salt-tolerant isolates characterized by tolerance to acidity with high growth in alkaline conditions. Group 2 covered slow-growing acid-sensitive isolates that high salinity negatively affected their growth. Results of AHC and MFA evidenced that bacterial diversity of endosymbiont isolates showed high level of similarity with RS, a proof that they are rhizobial strains. In concordance with phylogenitic results isolates are closely related to different rhizobia species The salt tolerant and heavy metals resistance patterns found among the indigenous rhizobial strains associated to G. microcephala and A. uniflorum are reflecting the environmental stresses pressure and make the strains good candidates for plant successful inoculation in arid areas. Our findings indicate that both fast- and slow-growing rhizobia nodulated G. cinerea growing in arid and semiarid regions of northeast Algeria. These rhizobia are poly-extremophiles adapted to diverse environmental stresses and linked to Beta and Gammaprotéobacteria. Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/DEK7408.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11060 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité DEK/7408 DEK/7408 Thèse Bibliothèque principale Thèses Disponible
Titre : Caractérisation phénotypique et génotypique des bactéries nodulant la légumineuse du genre Phaseolus Type de document : texte imprimé Auteurs : Mouna Saoudi, Auteur ; Yacine Benhizia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 118 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Rhizobium Phaseolus P.vulgaris P.coccineus micro-symbion Bacillus subtilis haricot bean المجهري عقيدات العضو التكافلي Index. décimale : 570 Sciences de la vie. Biologie Résumé : The symbiosis Rhizobium-legumes is a natural process, which can benefit the plant by the
assimilation of atmospheric nitrogen. In Algeria, beans or Phaseolus are leguminous plants
that play a prominent role in human nutrition, thanks to its protein and carbohydrate value,
and for its economic value for the country and the citizen. In order to improve the outcomeof
this legume, 86 isolates were collected from the root nodules of two species of Phaseolus; P.
vulgaris and P. coccineus grown in five orchads located in Eastern and Northeastern Algeria.
These isolates are characterized by a phenotypic study (physiological, biochemical and
nutritional tests) with the reference strains, which gives a description comparing
withRhizobium. Also our isolates present a positive reaction to direct contact with the
bacteriophages, that permits their use as markers for the identification of bacteria.
A nodulation test under bacteriologically controlled conditions is carried out by
demonstrating the ability of the isolates to nodulate the roots of the host plant which has
shown the formation of white nodules, indicating that our isolates are infectious and they
(This formation) confirm the symbiotic relationship between the host plant and the micro
symbion which is a specific relationship. The molecular study carried out confirmed
the belonging of the T8 isolate to the Bacillus subtilis species with a similarity of 98%.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/SAO7115.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10486 Caractérisation phénotypique et génotypique des bactéries nodulant la légumineuse du genre Phaseolus [texte imprimé] / Mouna Saoudi, Auteur ; Yacine Benhizia, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 118 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Rhizobium Phaseolus P.vulgaris P.coccineus micro-symbion Bacillus subtilis haricot bean المجهري عقيدات العضو التكافلي Index. décimale : 570 Sciences de la vie. Biologie Résumé : The symbiosis Rhizobium-legumes is a natural process, which can benefit the plant by the
assimilation of atmospheric nitrogen. In Algeria, beans or Phaseolus are leguminous plants
that play a prominent role in human nutrition, thanks to its protein and carbohydrate value,
and for its economic value for the country and the citizen. In order to improve the outcomeof
this legume, 86 isolates were collected from the root nodules of two species of Phaseolus; P.
vulgaris and P. coccineus grown in five orchads located in Eastern and Northeastern Algeria.
These isolates are characterized by a phenotypic study (physiological, biochemical and
nutritional tests) with the reference strains, which gives a description comparing
withRhizobium. Also our isolates present a positive reaction to direct contact with the
bacteriophages, that permits their use as markers for the identification of bacteria.
A nodulation test under bacteriologically controlled conditions is carried out by
demonstrating the ability of the isolates to nodulate the roots of the host plant which has
shown the formation of white nodules, indicating that our isolates are infectious and they
(This formation) confirm the symbiotic relationship between the host plant and the micro
symbion which is a specific relationship. The molecular study carried out confirmed
the belonging of the T8 isolate to the Bacillus subtilis species with a similarity of 98%.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/SAO7115.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10486 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité SAO/7115 SAO/7115 Thèse Bibliothèque principale Thèses Disponible
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