2 résultat(s) recherche sur le tag 'Phoenix dactylifera'    

Biological activities of Phoenix dactylifera and Treg in Rheumatoid arthritis induced by hyperhomocysteinemia and formalin and on tumoral process / Houssem Eddine Kehili  

Public ISBD Titre : Biological activities of Phoenix dactylifera and Treg in Rheumatoid arthritis induced by hyperhomocysteinemia and formalin and on tumoral process Type de document : texte imprimé Auteurs : Houssem Eddine Kehili, Auteur ; sakina Zerizer, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 173 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Anglais (eng) Catégories : Français - Anglais Biologie Tags : Phoenix dactylifera  activité immunomodulatrice  glutathion réduit  inflammation  hyperhomocystéinémie  activité anti-arthrite  cancer  immunomodulatory activity  GSH glutathione  hyperhomocysteinemia  anti-arthritis activity  ????? dactylifera Phoenix  ?????? ???????  ?????????GSH  ????????  ??? ????????????  ???? ?????? ???????  ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Inflammation plays an important role in various diseases, such as rheumatoid arthritis, atherosclerosis and asthma, which all show a high prevalence globally and the absence of the immune tolerance which is insured by the lymphocyte Treg. This tolerance protects the selfantigens from the immune system’s reaction. In addition, some chronic inflammatory diseases are recently found to be associated with an increased levels of hs-CRP and plasma homocysteine. In the present study, we evaluated the in vivo, the effect of two varieties of Phoenix dactylifera (Azarza variety grown in Ghardaia and variety Homayra grown in Adrar) on the toxicity using the up and down test, the immunomodulatory activity of the extracts using the carbon clearance from the blood, antioxidant by the measurement of the GSH from liver’s homogenate, anti-inflammatory activity and anti-arthritis by the formalin and L-methionine test in Albino mice. Also we have carried a study in vitro to establish the anti-proliferative effect of the methanolic and acetone extracts dates on liver cancer cell line (HepG2), breast cancer cell line (MCF7) and healthy cells, endothelial cells (HUVEC) and hepatocytes (hNHEPS). In addition to this work, we tested the effect of Phoenix dactylifera extracts on apoptotic genes (Bcl2 and BAX) and on the differentiation of human naïve lymphocyte T CD4+ into regulatory lymphocytes Treg. The results showed that the extracts of Phoenix dactylifera have no toxic effect at a dose of 2000mg/kg, also we found that the extracts increased significantly the phagocytic activity of the reticuloendothelial system, and release of glutathione reduced (GSH) from the liver. Furthermore, the extracts of Phoenix dactylifera studied reveal correction on the inflammation associated with hyperhomocysteinemia presented by a significant decrease in the size of the edema induced by formalin injection and a significant decrease in the hs- CRPvalues and homocysteine Hcy (p ≤ 0.05) in mice treated compared to controls, where it was observed that the administration of L-methionine 400 mg/kg caused a worsening of inflammation presented by a significant increase in protein C-reactive (CRP) p≤0,05 and a significant increase in homocysteine (Hcy) p≤0,05. Our results demonstrate a significant inhibition of edema of mice paws treated with our extracts with a decrease in the Anti-CCP values. The results indicate that treatment with six different concentrations (2 mg/100mL, 4 mg/100mL, 7.5 mg/100mL, 8 mg/100mL, 16 mg/1 00mL and 20 mg/100mL) inhibited the growth of tumor cells but had no toxicity to healthy cells. Diplôme : Doctorat En ligne : ../theses/biologie/KEH6985.pdf Format de la ressource électronique : pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10409 Biological activities of Phoenix dactylifera and Treg in Rheumatoid arthritis induced by hyperhomocysteinemia and formalin and on tumoral process [texte imprimé] / Houssem Eddine Kehili, Auteur ; sakina Zerizer, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2016 . - 173 f. ; 30 cm. 2 copies imprimées disponibles Langues : Anglais (eng) Catégories : Français - Anglais Biologie Tags : Phoenix dactylifera  activité immunomodulatrice  glutathion réduit  inflammation  hyperhomocystéinémie  activité anti-arthrite  cancer  immunomodulatory activity  GSH glutathione  hyperhomocysteinemia  anti-arthritis activity  ????? dactylifera Phoenix  ?????? ???????  ?????????GSH  ????????  ??? ????????????  ???? ?????? ???????  ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Inflammation plays an important role in various diseases, such as rheumatoid arthritis, atherosclerosis and asthma, which all show a high prevalence globally and the absence of the immune tolerance which is insured by the lymphocyte Treg. This tolerance protects the selfantigens from the immune system’s reaction. In addition, some chronic inflammatory diseases are recently found to be associated with an increased levels of hs-CRP and plasma homocysteine. In the present study, we evaluated the in vivo, the effect of two varieties of Phoenix dactylifera (Azarza variety grown in Ghardaia and variety Homayra grown in Adrar) on the toxicity using the up and down test, the immunomodulatory activity of the extracts using the carbon clearance from the blood, antioxidant by the measurement of the GSH from liver’s homogenate, anti-inflammatory activity and anti-arthritis by the formalin and L-methionine test in Albino mice. Also we have carried a study in vitro to establish the anti-proliferative effect of the methanolic and acetone extracts dates on liver cancer cell line (HepG2), breast cancer cell line (MCF7) and healthy cells, endothelial cells (HUVEC) and hepatocytes (hNHEPS). In addition to this work, we tested the effect of Phoenix dactylifera extracts on apoptotic genes (Bcl2 and BAX) and on the differentiation of human naïve lymphocyte T CD4+ into regulatory lymphocytes Treg. The results showed that the extracts of Phoenix dactylifera have no toxic effect at a dose of 2000mg/kg, also we found that the extracts increased significantly the phagocytic activity of the reticuloendothelial system, and release of glutathione reduced (GSH) from the liver. Furthermore, the extracts of Phoenix dactylifera studied reveal correction on the inflammation associated with hyperhomocysteinemia presented by a significant decrease in the size of the edema induced by formalin injection and a significant decrease in the hs- CRPvalues and homocysteine Hcy (p ≤ 0.05) in mice treated compared to controls, where it was observed that the administration of L-methionine 400 mg/kg caused a worsening of inflammation presented by a significant increase in protein C-reactive (CRP) p≤0,05 and a significant increase in homocysteine (Hcy) p≤0,05. Our results demonstrate a significant inhibition of edema of mice paws treated with our extracts with a decrease in the Anti-CCP values. The results indicate that treatment with six different concentrations (2 mg/100mL, 4 mg/100mL, 7.5 mg/100mL, 8 mg/100mL, 16 mg/1 00mL and 20 mg/100mL) inhibited the growth of tumor cells but had no toxicity to healthy cells. Diplôme : Doctorat En ligne : ../theses/biologie/KEH6985.pdf Format de la ressource électronique : pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10409

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
KEH/6985	KEH/6985	Thèse	Bibliothèque principale	Thèses	Disponible

Biodiversité de la flore bactérienne du palmier dattier poussant au sud est Algérien. Effet du climat sur le comportement de cette flore. / Wissame Aimene  

Public ISBD Titre : Biodiversité de la flore bactérienne du palmier dattier poussant au sud est Algérien. Effet du climat sur le comportement de cette flore. Type de document : texte imprimé Auteurs : Wissame Aimene, Auteur ; Yacine Benhizia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 343 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais Biologie Tags : Phoenix dactylifera  Phyllosphère  ADNr 16S  diversité bactérienne  phyllosphere  16S rDNA  bacterial diversity  ?????? ????????ADNr 16S Index. décimale : 570 Sciences de la vie. Biologie Résumé : Bacterial flora of the three parts of the date palm (Phoenix dactylifera L.) (Leaves, fruits and trunk) was isolated and then identified following phenotypic and molecular tests (using 16S rDNA). Evaluation of the bacterial population through the different seasons (in 2012) was carried out to demonstrate the temporal dynamics of the cultivable population. Statistical analysis revealed significant differences in the distribution of bacterial strains with increase or decrease of bacterial levels during the different seasons. Morphological characters and simple orientation tests allowed selecting 78 representative isolates from more than 200. The results of phenotypic identification made it possible to classify the majority of isolates at the genus level. As well as, the study of the effect of a biotic factors on the growth of isolates revealed that the tested isolates tolerate and survive under severe a biotic conditions. According to the partial analysis of the gene coding for 16S rRNA, 2 isolates were identified perfectly with 100% similarities, some isolates were identified between 97% to 99% similarity to the strains conserved in Genbank, while others strains were identified between 78% to 96%. Overall, there were 33 bacterial species belonging to 16 genera. The most common genera in all parts of the tree are Bacillus (36.84%), Pseudemonas and Enterobacter representing 17.1% for each, but however its distribution is heterogeneous. The genus Lysinibacillus is the third most isolated genus (10.52%). Unlike other genera that remain minority such as Staphylococcus, Serratia, Microbacterium, Kluyvera, Arthrobacter, Stenotrophomonas, Exiguobacterium, Micrococcus, Klebsiella, Massilia, Erwinia, and Brevibacterium. This work gave for the first time in Algeria a global idea about the diversity of the date palm bacterial flora and highlighted the impact of climate on the dynamics of this flora. Diplôme : Doctorat En ligne : ../theses/biologie/AIM7296.pdf Format de la ressource électronique : pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10911 Biodiversité de la flore bactérienne du palmier dattier poussant au sud est Algérien. Effet du climat sur le comportement de cette flore. [texte imprimé] / Wissame Aimene, Auteur ; Yacine Benhizia, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2018 . - 343 f. ; 30 cm. 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais Biologie Tags : Phoenix dactylifera  Phyllosphère  ADNr 16S  diversité bactérienne  phyllosphere  16S rDNA  bacterial diversity  ?????? ????????ADNr 16S Index. décimale : 570 Sciences de la vie. Biologie Résumé : Bacterial flora of the three parts of the date palm (Phoenix dactylifera L.) (Leaves, fruits and trunk) was isolated and then identified following phenotypic and molecular tests (using 16S rDNA). Evaluation of the bacterial population through the different seasons (in 2012) was carried out to demonstrate the temporal dynamics of the cultivable population. Statistical analysis revealed significant differences in the distribution of bacterial strains with increase or decrease of bacterial levels during the different seasons. Morphological characters and simple orientation tests allowed selecting 78 representative isolates from more than 200. The results of phenotypic identification made it possible to classify the majority of isolates at the genus level. As well as, the study of the effect of a biotic factors on the growth of isolates revealed that the tested isolates tolerate and survive under severe a biotic conditions. According to the partial analysis of the gene coding for 16S rRNA, 2 isolates were identified perfectly with 100% similarities, some isolates were identified between 97% to 99% similarity to the strains conserved in Genbank, while others strains were identified between 78% to 96%. Overall, there were 33 bacterial species belonging to 16 genera. The most common genera in all parts of the tree are Bacillus (36.84%), Pseudemonas and Enterobacter representing 17.1% for each, but however its distribution is heterogeneous. The genus Lysinibacillus is the third most isolated genus (10.52%). Unlike other genera that remain minority such as Staphylococcus, Serratia, Microbacterium, Kluyvera, Arthrobacter, Stenotrophomonas, Exiguobacterium, Micrococcus, Klebsiella, Massilia, Erwinia, and Brevibacterium. This work gave for the first time in Algeria a global idea about the diversity of the date palm bacterial flora and highlighted the impact of climate on the dynamics of this flora. Diplôme : Doctorat En ligne : ../theses/biologie/AIM7296.pdf Format de la ressource électronique : pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10911

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
AIM/7296	AIM/7296	Thèse	Bibliothèque principale	Thèses	Disponible