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Titre : Effect of bioactive molecules extracted from medicinal plants on inflammation induced by hyperhomocysteinemia and tumoral process Type de document : texte imprimé Auteurs : Assia Benmebarek, Auteur ; S Zerizer, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2014 Importance : 231 f. Note générale : 2 copies imprimées disponibles
Langues : Anglais (eng) Catégories : Français - Anglais
BiologieTags : Hyperhomocysteinemia atherogenesis inflammation carcinogenesis Stachys mialhesi Stachys ocymastrum immunomodulatory activity Hyperhomocysteinémie athérogénèse carcinogénèse activité immunomodulatrice ????? ???????????? ???????? ???? ???????? ???????? ??????? ??????? ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Hyperhomocysteinemia is an important risk factor in cardiovascular diseases and may also be implicated in cancer biology. Elevated plasma homocysteine (Hcy) levels may mediate long-term oxidative damage at the vascular interface and there appear to be a mechanism by which they may also be carcinogenic. In addition, immunostimulatory therapy is one solution to enhance the immunity, however, the immunopharmacologic activities of herbal extracts may be antagonistic, they can be immunosupressive or immunostimulating. In the present study, we evaluated the in vivo effect of S. mialhesi extract on the inflammation induced by hyperhomocysteinemia and damages induced to the aorta, heart, liver, thymus and spleen. Another study was carried on the immunomodulatory potential of S. mialhesi
and S. ocymastrum on the phagocytic activity which was evaluated using the Carbon Clearance Assay. The results obtained showed that plasma hs-CRP concentrations were elevated significantly after the administration of L-methionine in high doses to mice (200mg/kg). Also, the loss and degeneration of endothelium, fenestration, foam cells formation, and a change in the appearance of smooth muscle cells nuclei from a fusiform nuclei aspect to a rounded one, were observed in the media of the aorta as well as the alteration of the cardiac muscle, liver necrosis, lymphocytes membrane destruction in the thymus, and a hypertrophy of the spleen white pulp. Our research also revealed that at different doses, S. mialhesi extract increased the reticuloendothelial system (RES)
phagocytic activity in a dose dependant manner, in contrast to S. ocymastrum who exhibited a biphasic dose response effect. Hcy initiated inflammation and mediated early atherogenesis lesions through increased oxidant stress, and the treatment with S. mialhesi extract at a dose of (50mg/kg) prevented the alterations. Also, S. mialhesi extract exhibited a dose-dependent immunostimulatory effect on the RES and S. ocymastrum extract appeared immunostimulatory at low concentrations and immunosuppressive at high concentrations. Moreover, in the present thesis, we performed a review study which demonstrated that Hcy may mediate carcinogenesis namely through folate deficiency, oxidative stress, aberrant DNA methylation and the production of homocysteine thiolactone.
Diplôme : Doctorat En ligne : ../theses/biologie/BEN6973.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10371 Effect of bioactive molecules extracted from medicinal plants on inflammation induced by hyperhomocysteinemia and tumoral process [texte imprimé] / Assia Benmebarek, Auteur ; S Zerizer, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2014 . - 231 f.
2 copies imprimées disponibles
Langues : Anglais (eng)
Catégories : Français - Anglais
BiologieTags : Hyperhomocysteinemia atherogenesis inflammation carcinogenesis Stachys mialhesi Stachys ocymastrum immunomodulatory activity Hyperhomocysteinémie athérogénèse carcinogénèse activité immunomodulatrice ????? ???????????? ???????? ???? ???????? ???????? ??????? ??????? ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Hyperhomocysteinemia is an important risk factor in cardiovascular diseases and may also be implicated in cancer biology. Elevated plasma homocysteine (Hcy) levels may mediate long-term oxidative damage at the vascular interface and there appear to be a mechanism by which they may also be carcinogenic. In addition, immunostimulatory therapy is one solution to enhance the immunity, however, the immunopharmacologic activities of herbal extracts may be antagonistic, they can be immunosupressive or immunostimulating. In the present study, we evaluated the in vivo effect of S. mialhesi extract on the inflammation induced by hyperhomocysteinemia and damages induced to the aorta, heart, liver, thymus and spleen. Another study was carried on the immunomodulatory potential of S. mialhesi
and S. ocymastrum on the phagocytic activity which was evaluated using the Carbon Clearance Assay. The results obtained showed that plasma hs-CRP concentrations were elevated significantly after the administration of L-methionine in high doses to mice (200mg/kg). Also, the loss and degeneration of endothelium, fenestration, foam cells formation, and a change in the appearance of smooth muscle cells nuclei from a fusiform nuclei aspect to a rounded one, were observed in the media of the aorta as well as the alteration of the cardiac muscle, liver necrosis, lymphocytes membrane destruction in the thymus, and a hypertrophy of the spleen white pulp. Our research also revealed that at different doses, S. mialhesi extract increased the reticuloendothelial system (RES)
phagocytic activity in a dose dependant manner, in contrast to S. ocymastrum who exhibited a biphasic dose response effect. Hcy initiated inflammation and mediated early atherogenesis lesions through increased oxidant stress, and the treatment with S. mialhesi extract at a dose of (50mg/kg) prevented the alterations. Also, S. mialhesi extract exhibited a dose-dependent immunostimulatory effect on the RES and S. ocymastrum extract appeared immunostimulatory at low concentrations and immunosuppressive at high concentrations. Moreover, in the present thesis, we performed a review study which demonstrated that Hcy may mediate carcinogenesis namely through folate deficiency, oxidative stress, aberrant DNA methylation and the production of homocysteine thiolactone.
Diplôme : Doctorat En ligne : ../theses/biologie/BEN6973.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10371 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEN/6973 BEN/6973 Thèse Bibliothèque principale Thèses Disponible
Titre : Biological activities of Phoenix dactylifera and Treg in Rheumatoid arthritis induced by hyperhomocysteinemia and formalin and on tumoral process Type de document : texte imprimé Auteurs : Houssem Eddine Kehili, Auteur ; sakina Zerizer, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 173 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Anglais (eng) Catégories : Français - Anglais
BiologieTags : Phoenix dactylifera activité immunomodulatrice glutathion réduit inflammation hyperhomocystéinémie activité anti-arthrite cancer immunomodulatory activity GSH glutathione hyperhomocysteinemia anti-arthritis activity ????? dactylifera Phoenix ?????? ??????? ?????????GSH ???????? ??? ???????????? ???? ?????? ??????? ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Inflammation plays an important role in various diseases, such as rheumatoid arthritis, atherosclerosis and asthma, which all show a high prevalence globally and the absence of the immune tolerance which is insured by the lymphocyte Treg. This tolerance protects the selfantigens from the immune system’s reaction. In addition, some chronic inflammatory diseases are recently found to be associated with an increased levels of hs-CRP and plasma homocysteine.
In the present study, we evaluated the in vivo, the effect of two varieties of Phoenix
dactylifera (Azarza variety grown in Ghardaia and variety Homayra grown in Adrar) on the toxicity using the up and down test, the immunomodulatory activity of the extracts using the carbon clearance from the blood, antioxidant by the measurement of the GSH from liver’s homogenate, anti-inflammatory activity and anti-arthritis by the formalin and L-methionine test in Albino mice. Also we have carried a study in vitro to establish the anti-proliferative effect of the methanolic and acetone extracts dates on liver cancer cell line (HepG2), breast cancer cell line (MCF7) and healthy cells, endothelial cells (HUVEC) and hepatocytes (hNHEPS). In addition to this work, we tested the effect of Phoenix dactylifera extracts on apoptotic genes (Bcl2 and BAX) and on the differentiation of human naïve lymphocyte T CD4+ into regulatory lymphocytes Treg.
The results showed that the extracts of Phoenix dactylifera have no toxic effect at a
dose of 2000mg/kg, also we found that the extracts increased significantly the phagocytic activity of the reticuloendothelial system, and release of glutathione reduced (GSH) from the liver. Furthermore, the extracts of Phoenix dactylifera studied reveal correction on the inflammation associated with hyperhomocysteinemia presented by a significant decrease in the size of the edema induced by formalin injection and a significant decrease in the hs- CRPvalues and homocysteine Hcy (p ≤ 0.05) in mice treated compared to controls, where it was observed that the administration of L-methionine 400 mg/kg caused a worsening of inflammation presented by a significant increase in protein C-reactive (CRP) p≤0,05 and a significant increase in homocysteine (Hcy) p≤0,05. Our results demonstrate a significant inhibition of edema of mice paws treated with our extracts with a decrease in the Anti-CCP values.
The results indicate that treatment with six different concentrations (2 mg/100mL, 4
mg/100mL, 7.5 mg/100mL, 8 mg/100mL, 16 mg/1 00mL and 20 mg/100mL) inhibited the
growth of tumor cells but had no toxicity to healthy cells.
Diplôme : Doctorat En ligne : ../theses/biologie/KEH6985.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10409 Biological activities of Phoenix dactylifera and Treg in Rheumatoid arthritis induced by hyperhomocysteinemia and formalin and on tumoral process [texte imprimé] / Houssem Eddine Kehili, Auteur ; sakina Zerizer, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2016 . - 173 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Anglais (eng)
Catégories : Français - Anglais
BiologieTags : Phoenix dactylifera activité immunomodulatrice glutathion réduit inflammation hyperhomocystéinémie activité anti-arthrite cancer immunomodulatory activity GSH glutathione hyperhomocysteinemia anti-arthritis activity ????? dactylifera Phoenix ?????? ??????? ?????????GSH ???????? ??? ???????????? ???? ?????? ??????? ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Inflammation plays an important role in various diseases, such as rheumatoid arthritis, atherosclerosis and asthma, which all show a high prevalence globally and the absence of the immune tolerance which is insured by the lymphocyte Treg. This tolerance protects the selfantigens from the immune system’s reaction. In addition, some chronic inflammatory diseases are recently found to be associated with an increased levels of hs-CRP and plasma homocysteine.
In the present study, we evaluated the in vivo, the effect of two varieties of Phoenix
dactylifera (Azarza variety grown in Ghardaia and variety Homayra grown in Adrar) on the toxicity using the up and down test, the immunomodulatory activity of the extracts using the carbon clearance from the blood, antioxidant by the measurement of the GSH from liver’s homogenate, anti-inflammatory activity and anti-arthritis by the formalin and L-methionine test in Albino mice. Also we have carried a study in vitro to establish the anti-proliferative effect of the methanolic and acetone extracts dates on liver cancer cell line (HepG2), breast cancer cell line (MCF7) and healthy cells, endothelial cells (HUVEC) and hepatocytes (hNHEPS). In addition to this work, we tested the effect of Phoenix dactylifera extracts on apoptotic genes (Bcl2 and BAX) and on the differentiation of human naïve lymphocyte T CD4+ into regulatory lymphocytes Treg.
The results showed that the extracts of Phoenix dactylifera have no toxic effect at a
dose of 2000mg/kg, also we found that the extracts increased significantly the phagocytic activity of the reticuloendothelial system, and release of glutathione reduced (GSH) from the liver. Furthermore, the extracts of Phoenix dactylifera studied reveal correction on the inflammation associated with hyperhomocysteinemia presented by a significant decrease in the size of the edema induced by formalin injection and a significant decrease in the hs- CRPvalues and homocysteine Hcy (p ≤ 0.05) in mice treated compared to controls, where it was observed that the administration of L-methionine 400 mg/kg caused a worsening of inflammation presented by a significant increase in protein C-reactive (CRP) p≤0,05 and a significant increase in homocysteine (Hcy) p≤0,05. Our results demonstrate a significant inhibition of edema of mice paws treated with our extracts with a decrease in the Anti-CCP values.
The results indicate that treatment with six different concentrations (2 mg/100mL, 4
mg/100mL, 7.5 mg/100mL, 8 mg/100mL, 16 mg/1 00mL and 20 mg/100mL) inhibited the
growth of tumor cells but had no toxicity to healthy cells.
Diplôme : Doctorat En ligne : ../theses/biologie/KEH6985.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10409 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KEH/6985 KEH/6985 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude des facteurs de risque génétiques de la maladie thromboembolique veineuse chez une population de l’Est-Algérien Type de document : texte imprimé Auteurs : Samira Moussaoui, Auteur ; N. Abadi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 141 f. Format : 30 cm. Note générale : 2 copies imprimes disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biochimie et Biologie Moléculaire et Cellulaire gene polymorphisms venous thromboembolism disease thrombophilia hyperhomocysteinemia MTHFR C677T JAK2-V617F calreticulin hemostasis
polymophismes génétiques maladie thromboembolique veineuse thrombophilie hypperhomocystéinémie C677T de la MTHFR calréticuline hémostase ??????? ???????? ??????? ??????? ???????? ???? ?????? ?????? ???? ?????????????
???????Index. décimale : 570 Sciences de la vie. Biologie Résumé : Venous thromboembolism (VTE) is a multifactorial disease in which the genetic
component is important.Many genetic risk factors have been identified for causing VTE.
Most of them affect the function of natural anticoagulant pathways, particularly the protein
C system, although recent studies suggest a role of components of the hematopoietic
pathway in the etiology of venous thrombosis.
The aim of the study is to determine the risk of VTE associated with (prothrombin
G20210A, factor V Leiden G1691A, MTHFR C677T) polymorphisms, (protein C, protein
S, antithrombin III) deficiencies and hyperhomocysteinemia in a population of thrombotic
patients compared to a control population. On the other hand, our study tends to evaluate the
status of JAK2V617F and calreticulin mutations among thrombotic patients in eastern
Algeria.
Methods: 121 cases with VTE and 146 healthy controls were recruited in this study.
Polymorphisms of FVL G1691A, prothrombin G20210A and MTHFR C677T were
genotyped by PCR-RFLP. JAK2-V617F and calreticlin mutations were analysed by q-PCR
and PCR followed by capillary electrophoresis sequencing respectively. The rate of
coagulation inhibitors and tHcy levels were determined by Stago and immmulite 2000
instruments respectively; then hereditary deficiencies were identified.
Results: Of all cases and controls, none was a carrier of the antithrombin III deficiency,
prothrombin gene G20210A and calreticulin mutations. Only one case reported having a
positive JAK2 mutation (mutant allele burden was 15%). The univariate analysis results in
a significant association of the mutation (GA / AA) of factor V Leiden (OR = 9.4, 95% CI
= 2.1, 42.3, P = 0.003) and protein S deficiency (OR = 16.9, 95% CI = 2.1; 132.8, P = 0.007)
in VTE. The association remained significant even after adjustment for age and sex in the
multivariate analysis. The odds ratio of protein C deficiency was slightly higher (OR = 6.4,
95% CI = 0.7, 55.5), however it is not statistically significant (P = 0.091). In addition, this
study showed that there was no significant association between MTHFR C677T mutation,
hyperhomocysteinemia and risk of venous thrombosis
Conclusion: Our study supports the idea that the FV Leiden and protein S deficiency are
independent prothrombotic risk factors in the population of eastern Algeria. The somatic
mutation of JAK2 V617F and calreticulin are less frequent causes of VTE, thus routine
testing for these mutations is not recommended.Diplôme : Doctorat En ligne : ../theses/biologie/MOU6848.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10145 Etude des facteurs de risque génétiques de la maladie thromboembolique veineuse chez une population de l’Est-Algérien [texte imprimé] / Samira Moussaoui, Auteur ; N. Abadi, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2016 . - 141 f. ; 30 cm.
2 copies imprimes disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biochimie et Biologie Moléculaire et Cellulaire gene polymorphisms venous thromboembolism disease thrombophilia hyperhomocysteinemia MTHFR C677T JAK2-V617F calreticulin hemostasis
polymophismes génétiques maladie thromboembolique veineuse thrombophilie hypperhomocystéinémie C677T de la MTHFR calréticuline hémostase ??????? ???????? ??????? ??????? ???????? ???? ?????? ?????? ???? ?????????????
???????Index. décimale : 570 Sciences de la vie. Biologie Résumé : Venous thromboembolism (VTE) is a multifactorial disease in which the genetic
component is important.Many genetic risk factors have been identified for causing VTE.
Most of them affect the function of natural anticoagulant pathways, particularly the protein
C system, although recent studies suggest a role of components of the hematopoietic
pathway in the etiology of venous thrombosis.
The aim of the study is to determine the risk of VTE associated with (prothrombin
G20210A, factor V Leiden G1691A, MTHFR C677T) polymorphisms, (protein C, protein
S, antithrombin III) deficiencies and hyperhomocysteinemia in a population of thrombotic
patients compared to a control population. On the other hand, our study tends to evaluate the
status of JAK2V617F and calreticulin mutations among thrombotic patients in eastern
Algeria.
Methods: 121 cases with VTE and 146 healthy controls were recruited in this study.
Polymorphisms of FVL G1691A, prothrombin G20210A and MTHFR C677T were
genotyped by PCR-RFLP. JAK2-V617F and calreticlin mutations were analysed by q-PCR
and PCR followed by capillary electrophoresis sequencing respectively. The rate of
coagulation inhibitors and tHcy levels were determined by Stago and immmulite 2000
instruments respectively; then hereditary deficiencies were identified.
Results: Of all cases and controls, none was a carrier of the antithrombin III deficiency,
prothrombin gene G20210A and calreticulin mutations. Only one case reported having a
positive JAK2 mutation (mutant allele burden was 15%). The univariate analysis results in
a significant association of the mutation (GA / AA) of factor V Leiden (OR = 9.4, 95% CI
= 2.1, 42.3, P = 0.003) and protein S deficiency (OR = 16.9, 95% CI = 2.1; 132.8, P = 0.007)
in VTE. The association remained significant even after adjustment for age and sex in the
multivariate analysis. The odds ratio of protein C deficiency was slightly higher (OR = 6.4,
95% CI = 0.7, 55.5), however it is not statistically significant (P = 0.091). In addition, this
study showed that there was no significant association between MTHFR C677T mutation,
hyperhomocysteinemia and risk of venous thrombosis
Conclusion: Our study supports the idea that the FV Leiden and protein S deficiency are
independent prothrombotic risk factors in the population of eastern Algeria. The somatic
mutation of JAK2 V617F and calreticulin are less frequent causes of VTE, thus routine
testing for these mutations is not recommended.Diplôme : Doctorat En ligne : ../theses/biologie/MOU6848.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10145 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MOU/6848 MOU/6848 Thèse Bibliothèque principale Thèses Disponible
