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Partager le résultat de cette recherche Interroger des sources externesBiological activities of Phoenix dactylifera and Treg in Rheumatoid arthritis induced by hyperhomocysteinemia and formalin and on tumoral process / Houssem Eddine Kehili
Titre : Biological activities of Phoenix dactylifera and Treg in Rheumatoid arthritis induced by hyperhomocysteinemia and formalin and on tumoral process Type de document : texte imprimé Auteurs : Houssem Eddine Kehili, Auteur ; sakina Zerizer, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 173 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Anglais (eng) Catégories : Français - Anglais
BiologieTags : Phoenix dactylifera activité immunomodulatrice glutathion réduit inflammation hyperhomocystéinémie activité anti-arthrite cancer immunomodulatory activity GSH glutathione hyperhomocysteinemia anti-arthritis activity ????? dactylifera Phoenix ?????? ??????? ?????????GSH ???????? ??? ???????????? ???? ?????? ??????? ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Inflammation plays an important role in various diseases, such as rheumatoid arthritis, atherosclerosis and asthma, which all show a high prevalence globally and the absence of the immune tolerance which is insured by the lymphocyte Treg. This tolerance protects the selfantigens from the immune system’s reaction. In addition, some chronic inflammatory diseases are recently found to be associated with an increased levels of hs-CRP and plasma homocysteine.
In the present study, we evaluated the in vivo, the effect of two varieties of Phoenix
dactylifera (Azarza variety grown in Ghardaia and variety Homayra grown in Adrar) on the toxicity using the up and down test, the immunomodulatory activity of the extracts using the carbon clearance from the blood, antioxidant by the measurement of the GSH from liver’s homogenate, anti-inflammatory activity and anti-arthritis by the formalin and L-methionine test in Albino mice. Also we have carried a study in vitro to establish the anti-proliferative effect of the methanolic and acetone extracts dates on liver cancer cell line (HepG2), breast cancer cell line (MCF7) and healthy cells, endothelial cells (HUVEC) and hepatocytes (hNHEPS). In addition to this work, we tested the effect of Phoenix dactylifera extracts on apoptotic genes (Bcl2 and BAX) and on the differentiation of human naïve lymphocyte T CD4+ into regulatory lymphocytes Treg.
The results showed that the extracts of Phoenix dactylifera have no toxic effect at a
dose of 2000mg/kg, also we found that the extracts increased significantly the phagocytic activity of the reticuloendothelial system, and release of glutathione reduced (GSH) from the liver. Furthermore, the extracts of Phoenix dactylifera studied reveal correction on the inflammation associated with hyperhomocysteinemia presented by a significant decrease in the size of the edema induced by formalin injection and a significant decrease in the hs- CRPvalues and homocysteine Hcy (p ≤ 0.05) in mice treated compared to controls, where it was observed that the administration of L-methionine 400 mg/kg caused a worsening of inflammation presented by a significant increase in protein C-reactive (CRP) p≤0,05 and a significant increase in homocysteine (Hcy) p≤0,05. Our results demonstrate a significant inhibition of edema of mice paws treated with our extracts with a decrease in the Anti-CCP values.
The results indicate that treatment with six different concentrations (2 mg/100mL, 4
mg/100mL, 7.5 mg/100mL, 8 mg/100mL, 16 mg/1 00mL and 20 mg/100mL) inhibited the
growth of tumor cells but had no toxicity to healthy cells.
Diplôme : Doctorat En ligne : ../theses/biologie/KEH6985.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10409 Biological activities of Phoenix dactylifera and Treg in Rheumatoid arthritis induced by hyperhomocysteinemia and formalin and on tumoral process [texte imprimé] / Houssem Eddine Kehili, Auteur ; sakina Zerizer, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2016 . - 173 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Anglais (eng)
Catégories : Français - Anglais
BiologieTags : Phoenix dactylifera activité immunomodulatrice glutathion réduit inflammation hyperhomocystéinémie activité anti-arthrite cancer immunomodulatory activity GSH glutathione hyperhomocysteinemia anti-arthritis activity ????? dactylifera Phoenix ?????? ??????? ?????????GSH ???????? ??? ???????????? ???? ?????? ??????? ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Inflammation plays an important role in various diseases, such as rheumatoid arthritis, atherosclerosis and asthma, which all show a high prevalence globally and the absence of the immune tolerance which is insured by the lymphocyte Treg. This tolerance protects the selfantigens from the immune system’s reaction. In addition, some chronic inflammatory diseases are recently found to be associated with an increased levels of hs-CRP and plasma homocysteine.
In the present study, we evaluated the in vivo, the effect of two varieties of Phoenix
dactylifera (Azarza variety grown in Ghardaia and variety Homayra grown in Adrar) on the toxicity using the up and down test, the immunomodulatory activity of the extracts using the carbon clearance from the blood, antioxidant by the measurement of the GSH from liver’s homogenate, anti-inflammatory activity and anti-arthritis by the formalin and L-methionine test in Albino mice. Also we have carried a study in vitro to establish the anti-proliferative effect of the methanolic and acetone extracts dates on liver cancer cell line (HepG2), breast cancer cell line (MCF7) and healthy cells, endothelial cells (HUVEC) and hepatocytes (hNHEPS). In addition to this work, we tested the effect of Phoenix dactylifera extracts on apoptotic genes (Bcl2 and BAX) and on the differentiation of human naïve lymphocyte T CD4+ into regulatory lymphocytes Treg.
The results showed that the extracts of Phoenix dactylifera have no toxic effect at a
dose of 2000mg/kg, also we found that the extracts increased significantly the phagocytic activity of the reticuloendothelial system, and release of glutathione reduced (GSH) from the liver. Furthermore, the extracts of Phoenix dactylifera studied reveal correction on the inflammation associated with hyperhomocysteinemia presented by a significant decrease in the size of the edema induced by formalin injection and a significant decrease in the hs- CRPvalues and homocysteine Hcy (p ≤ 0.05) in mice treated compared to controls, where it was observed that the administration of L-methionine 400 mg/kg caused a worsening of inflammation presented by a significant increase in protein C-reactive (CRP) p≤0,05 and a significant increase in homocysteine (Hcy) p≤0,05. Our results demonstrate a significant inhibition of edema of mice paws treated with our extracts with a decrease in the Anti-CCP values.
The results indicate that treatment with six different concentrations (2 mg/100mL, 4
mg/100mL, 7.5 mg/100mL, 8 mg/100mL, 16 mg/1 00mL and 20 mg/100mL) inhibited the
growth of tumor cells but had no toxicity to healthy cells.
Diplôme : Doctorat En ligne : ../theses/biologie/KEH6985.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10409 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KEH/6985 KEH/6985 Thèse Bibliothèque principale Thèses Disponible
Titre : Effect of bioactive molecules extracted from medicinal plants on inflammation induced by hyperhomocysteinemia and tumoral process Type de document : texte imprimé Auteurs : Assia Benmebarek, Auteur ; S Zerizer, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2014 Importance : 231 f. Note générale : 2 copies imprimées disponibles
Langues : Anglais (eng) Catégories : Français - Anglais
BiologieTags : Hyperhomocysteinemia atherogenesis inflammation carcinogenesis Stachys mialhesi Stachys ocymastrum immunomodulatory activity Hyperhomocysteinémie athérogénèse carcinogénèse activité immunomodulatrice ????? ???????????? ???????? ???? ???????? ???????? ??????? ??????? ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Hyperhomocysteinemia is an important risk factor in cardiovascular diseases and may also be implicated in cancer biology. Elevated plasma homocysteine (Hcy) levels may mediate long-term oxidative damage at the vascular interface and there appear to be a mechanism by which they may also be carcinogenic. In addition, immunostimulatory therapy is one solution to enhance the immunity, however, the immunopharmacologic activities of herbal extracts may be antagonistic, they can be immunosupressive or immunostimulating. In the present study, we evaluated the in vivo effect of S. mialhesi extract on the inflammation induced by hyperhomocysteinemia and damages induced to the aorta, heart, liver, thymus and spleen. Another study was carried on the immunomodulatory potential of S. mialhesi
and S. ocymastrum on the phagocytic activity which was evaluated using the Carbon Clearance Assay. The results obtained showed that plasma hs-CRP concentrations were elevated significantly after the administration of L-methionine in high doses to mice (200mg/kg). Also, the loss and degeneration of endothelium, fenestration, foam cells formation, and a change in the appearance of smooth muscle cells nuclei from a fusiform nuclei aspect to a rounded one, were observed in the media of the aorta as well as the alteration of the cardiac muscle, liver necrosis, lymphocytes membrane destruction in the thymus, and a hypertrophy of the spleen white pulp. Our research also revealed that at different doses, S. mialhesi extract increased the reticuloendothelial system (RES)
phagocytic activity in a dose dependant manner, in contrast to S. ocymastrum who exhibited a biphasic dose response effect. Hcy initiated inflammation and mediated early atherogenesis lesions through increased oxidant stress, and the treatment with S. mialhesi extract at a dose of (50mg/kg) prevented the alterations. Also, S. mialhesi extract exhibited a dose-dependent immunostimulatory effect on the RES and S. ocymastrum extract appeared immunostimulatory at low concentrations and immunosuppressive at high concentrations. Moreover, in the present thesis, we performed a review study which demonstrated that Hcy may mediate carcinogenesis namely through folate deficiency, oxidative stress, aberrant DNA methylation and the production of homocysteine thiolactone.
Diplôme : Doctorat En ligne : ../theses/biologie/BEN6973.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10371 Effect of bioactive molecules extracted from medicinal plants on inflammation induced by hyperhomocysteinemia and tumoral process [texte imprimé] / Assia Benmebarek, Auteur ; S Zerizer, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2014 . - 231 f.
2 copies imprimées disponibles
Langues : Anglais (eng)
Catégories : Français - Anglais
BiologieTags : Hyperhomocysteinemia atherogenesis inflammation carcinogenesis Stachys mialhesi Stachys ocymastrum immunomodulatory activity Hyperhomocysteinémie athérogénèse carcinogénèse activité immunomodulatrice ????? ???????????? ???????? ???? ???????? ???????? ??????? ??????? ??????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Hyperhomocysteinemia is an important risk factor in cardiovascular diseases and may also be implicated in cancer biology. Elevated plasma homocysteine (Hcy) levels may mediate long-term oxidative damage at the vascular interface and there appear to be a mechanism by which they may also be carcinogenic. In addition, immunostimulatory therapy is one solution to enhance the immunity, however, the immunopharmacologic activities of herbal extracts may be antagonistic, they can be immunosupressive or immunostimulating. In the present study, we evaluated the in vivo effect of S. mialhesi extract on the inflammation induced by hyperhomocysteinemia and damages induced to the aorta, heart, liver, thymus and spleen. Another study was carried on the immunomodulatory potential of S. mialhesi
and S. ocymastrum on the phagocytic activity which was evaluated using the Carbon Clearance Assay. The results obtained showed that plasma hs-CRP concentrations were elevated significantly after the administration of L-methionine in high doses to mice (200mg/kg). Also, the loss and degeneration of endothelium, fenestration, foam cells formation, and a change in the appearance of smooth muscle cells nuclei from a fusiform nuclei aspect to a rounded one, were observed in the media of the aorta as well as the alteration of the cardiac muscle, liver necrosis, lymphocytes membrane destruction in the thymus, and a hypertrophy of the spleen white pulp. Our research also revealed that at different doses, S. mialhesi extract increased the reticuloendothelial system (RES)
phagocytic activity in a dose dependant manner, in contrast to S. ocymastrum who exhibited a biphasic dose response effect. Hcy initiated inflammation and mediated early atherogenesis lesions through increased oxidant stress, and the treatment with S. mialhesi extract at a dose of (50mg/kg) prevented the alterations. Also, S. mialhesi extract exhibited a dose-dependent immunostimulatory effect on the RES and S. ocymastrum extract appeared immunostimulatory at low concentrations and immunosuppressive at high concentrations. Moreover, in the present thesis, we performed a review study which demonstrated that Hcy may mediate carcinogenesis namely through folate deficiency, oxidative stress, aberrant DNA methylation and the production of homocysteine thiolactone.
Diplôme : Doctorat En ligne : ../theses/biologie/BEN6973.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10371 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEN/6973 BEN/6973 Thèse Bibliothèque principale Thèses Disponible
Titre : Evaluation de l'activité antioxydante et antimicrobienne de l'homogénat du gastéropode : Helix aspersa Type de document : texte imprimé Auteurs : Rima Hammoud, Auteur ; D. Naimi, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2013 Importance : 87 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : L-Méthionine hyperhomocystéinémie Helix aspersa extraits organiques bruts activité
antioxydante activité antimicrobienne L-Methionine hyerhomocysteinemia crude organic extracts antioxidant activity antimicrobial activity ?????????? ??????? ????? ?????? ?????? ??????? ???????? ????? ??????????????? L
?????? ?????? ???????? ????? ????????.Index. décimale : 570 Sciences de la vie. Biologie Résumé : In this study, we took an interest in the evaluation of the antioxidant and antimicrobial effects of Helix aspersa snail homogenate. In order to reach our goals, three principal stages were undertaken:
* Submission of rats at a high L-Methionine diet (400mg/kg of the weight of the rat/day) in order to induce an oxidative stress.
* Treatment of rats by Helix aspersa snail homogenate (100mg/kg of the weight of the rat/day) associated with a high dose of the L-Methionine.
* Evaluation of the antimicrobial activity of Helix aspersa snail crude organic extracts against three bacterial strains (Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603,Pseudomonas aeruginosa 13), twoo fungal strains (Aspergillus fumigatus CIP 1082.74,Trichophytum rubrum CIP 2043.92) and one strain of yeast (Candida albicans UMIP 88465) by the use of paper disk assay method.
The results of MDA and total protein mixtures showed that:
* On one hand, an increase of hepatic MD (p≤0,001) and plasmatic total protein levels (p≤0,0001) on rats that receiving a high dose of the L-Methionine, explaining thus theproceedings of lipid peroxidation phenomenons and the cellular proteins oxidation.
* On the other hand, a decrease of the levels of plasmatic (p≤0,0001) and hepatic MDA (p≤0,001), and plasmatic total proteins (p≤0,0001) following treatment of hyperhomocysteinemic rats with the snails homogenate. This allowed us to conclude that the Helix aspersa snail homogenate has antioxidant properties.
The microbiological test revealed an antibacterial activity of snails crude organic extracts prepared with distilled water.
Escherichia coli ATCC 25922 was revealed as the most sensitive of the three test-bacteria.
No anti fungal activity was determined.
The preliminary characterization of antibacterial molecules of Helix aspersa snail crude organic extracts allowed us to conclude that they can probably have protein linked and/or free nature.Diplôme : Magistère En ligne : ../theses/biologie/HAM6355.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=6321 Evaluation de l'activité antioxydante et antimicrobienne de l'homogénat du gastéropode : Helix aspersa [texte imprimé] / Rima Hammoud, Auteur ; D. Naimi, Directeur de thèse . - constantine (Route ain el bey, Algérie) : Université Constantine 1, 2013 . - 87 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : L-Méthionine hyperhomocystéinémie Helix aspersa extraits organiques bruts activité
antioxydante activité antimicrobienne L-Methionine hyerhomocysteinemia crude organic extracts antioxidant activity antimicrobial activity ?????????? ??????? ????? ?????? ?????? ??????? ???????? ????? ??????????????? L
?????? ?????? ???????? ????? ????????.Index. décimale : 570 Sciences de la vie. Biologie Résumé : In this study, we took an interest in the evaluation of the antioxidant and antimicrobial effects of Helix aspersa snail homogenate. In order to reach our goals, three principal stages were undertaken:
* Submission of rats at a high L-Methionine diet (400mg/kg of the weight of the rat/day) in order to induce an oxidative stress.
* Treatment of rats by Helix aspersa snail homogenate (100mg/kg of the weight of the rat/day) associated with a high dose of the L-Methionine.
* Evaluation of the antimicrobial activity of Helix aspersa snail crude organic extracts against three bacterial strains (Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603,Pseudomonas aeruginosa 13), twoo fungal strains (Aspergillus fumigatus CIP 1082.74,Trichophytum rubrum CIP 2043.92) and one strain of yeast (Candida albicans UMIP 88465) by the use of paper disk assay method.
The results of MDA and total protein mixtures showed that:
* On one hand, an increase of hepatic MD (p≤0,001) and plasmatic total protein levels (p≤0,0001) on rats that receiving a high dose of the L-Methionine, explaining thus theproceedings of lipid peroxidation phenomenons and the cellular proteins oxidation.
* On the other hand, a decrease of the levels of plasmatic (p≤0,0001) and hepatic MDA (p≤0,001), and plasmatic total proteins (p≤0,0001) following treatment of hyperhomocysteinemic rats with the snails homogenate. This allowed us to conclude that the Helix aspersa snail homogenate has antioxidant properties.
The microbiological test revealed an antibacterial activity of snails crude organic extracts prepared with distilled water.
Escherichia coli ATCC 25922 was revealed as the most sensitive of the three test-bacteria.
No anti fungal activity was determined.
The preliminary characterization of antibacterial molecules of Helix aspersa snail crude organic extracts allowed us to conclude that they can probably have protein linked and/or free nature.Diplôme : Magistère En ligne : ../theses/biologie/HAM6355.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=6321 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité HAM/6355 HAM/6355 Thèse Bibliothèque principale Thèses Disponible
