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Partager le résultat de cette recherche Interroger des sources externesIdentification des rhizobiums indigènes piégés à partir de la légumineuse Pisum sativum L. en Algérie, par approche polyphasique. / Meriem Gaci
Titre : Identification des rhizobiums indigènes piégés à partir de la légumineuse Pisum sativum L. en Algérie, par approche polyphasique. Type de document : texte imprimé Auteurs : Meriem Gaci, Auteur ; Yacine Benhizia, Directeur de thèse ; Francis Hynes Michael, Directeur de thèse Mention d'édition : 14/07/2021 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2021 Importance : 184 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Microbiologie : Écologie Microbienne Rhizobium Piégeage Diversité Pois fourrager Gène cpn60 Trapping Diversity Forage pea cpn60 gene ،Rhizobiumعملية إلتقاط السلالات التنوع البازلاء العلفية مورثَة cpn60 Index. décimale : 570 Sciences de la vie. Biologie Résumé : The legume family is economically important for food production and soil fertility. Many legumes have the ability to establish a symbiosis with soil bacteria called rhizobia. This interaction leads to the formation of a new root or in a few cases stem organ, the nodule, within which the bacteria can fix atmospheric nitrogen. This study aims to characterize phenotypically and genotypically the symbiotic bacteria nodulating the legume Pisum sativum L. The rhizobial strains living in the rhizosphere of forage pea were trapped in vitro from the collected soils. The study included seven sites located in Eastern and Central Algeria representing two agro-climatic zones, sub-humid and semi-arid. A collection of 94 isolates was obtained by direct isolation, or by bacterial trapping. 34 of them are rhizobial strains capable, with two exceptions, of establishing symbiosis with two cultivars of the forage pea (P. sativum cv. Sefrou and P. sativum cv. Trapper). Characterization using morphological tests, urea hydrolysis, nitrate reduction, cellulolytic and pectinolytic activity as well as growth at different temperatures gave a description comparable to that of Rhizobium. Different profiles of susceptibility to rhizobiophages indicated that the majority of isolates were likely to be members of the R. leguminosarum group of species. It is interesting to note that two isolates were resistant to all rhizobiophages. The strains revealed various plasmid profiles using Eckardt gels. 19 restriction profiles were distinguished based on PCR-RFLP of the cpn60 gene, and 15 distinct hybridization profiles were obtained using Southern blot analysis of nodABC genes. The 16S rRNA gene sequences showed that all isolates exhibited 99.85 % similarity to R. leguminosarum sv. viciae USDA 2370T, R. laguerreae FB206T and R. anhuiense CCBAU 23252T. Analysis of the cpn60 sequences revealed two groups. One of them was clustered with ≤ 99 % identity with R. laguerreae FB403. The second was clustered with ≤ 99 % identity with R. laguerreae FB206T. Our results suggest that the isolates may belong to the species R. laguerreae.
Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/GAC7823.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11655 Identification des rhizobiums indigènes piégés à partir de la légumineuse Pisum sativum L. en Algérie, par approche polyphasique. [texte imprimé] / Meriem Gaci, Auteur ; Yacine Benhizia, Directeur de thèse ; Francis Hynes Michael, Directeur de thèse . - 14/07/2021 . - جامعة الإخوة منتوري قسنطينة, 2021 . - 184 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Microbiologie : Écologie Microbienne Rhizobium Piégeage Diversité Pois fourrager Gène cpn60 Trapping Diversity Forage pea cpn60 gene ،Rhizobiumعملية إلتقاط السلالات التنوع البازلاء العلفية مورثَة cpn60 Index. décimale : 570 Sciences de la vie. Biologie Résumé : The legume family is economically important for food production and soil fertility. Many legumes have the ability to establish a symbiosis with soil bacteria called rhizobia. This interaction leads to the formation of a new root or in a few cases stem organ, the nodule, within which the bacteria can fix atmospheric nitrogen. This study aims to characterize phenotypically and genotypically the symbiotic bacteria nodulating the legume Pisum sativum L. The rhizobial strains living in the rhizosphere of forage pea were trapped in vitro from the collected soils. The study included seven sites located in Eastern and Central Algeria representing two agro-climatic zones, sub-humid and semi-arid. A collection of 94 isolates was obtained by direct isolation, or by bacterial trapping. 34 of them are rhizobial strains capable, with two exceptions, of establishing symbiosis with two cultivars of the forage pea (P. sativum cv. Sefrou and P. sativum cv. Trapper). Characterization using morphological tests, urea hydrolysis, nitrate reduction, cellulolytic and pectinolytic activity as well as growth at different temperatures gave a description comparable to that of Rhizobium. Different profiles of susceptibility to rhizobiophages indicated that the majority of isolates were likely to be members of the R. leguminosarum group of species. It is interesting to note that two isolates were resistant to all rhizobiophages. The strains revealed various plasmid profiles using Eckardt gels. 19 restriction profiles were distinguished based on PCR-RFLP of the cpn60 gene, and 15 distinct hybridization profiles were obtained using Southern blot analysis of nodABC genes. The 16S rRNA gene sequences showed that all isolates exhibited 99.85 % similarity to R. leguminosarum sv. viciae USDA 2370T, R. laguerreae FB206T and R. anhuiense CCBAU 23252T. Analysis of the cpn60 sequences revealed two groups. One of them was clustered with ≤ 99 % identity with R. laguerreae FB403. The second was clustered with ≤ 99 % identity with R. laguerreae FB206T. Our results suggest that the isolates may belong to the species R. laguerreae.
Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/GAC7823.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11655 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité GAC/7823 GAC/7823 Thèse Bibliothèque principale Thèses Disponible 
Titre : Diversité et structure génétique des populations de Rhizobium leguminosarum symbiovar viciae isolées du pois (Pisum sativum) et de la lentille (Lens culinaris) cultivés dans deux zones éco-climatiques subhumide et semi-aride de l'est Algérien Type de document : texte imprimé Auteurs : Nassira Riah, Auteur ; Abdelhamid Djekoun, Directeur de thèse ; Gisèle Laguerre, Directeur de thèse ; Philippe De Lajudie, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 131 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Pois lentille nodosités Rhizobium diversité symbiose structure génétique des
populations Algérie zones éco-climatiques Pea lentil nodules diversity symbiosis genetic structure of populations Algeria eco-climatic zones البازلا العدس العقد الجذرية الريزوبيوم التنوع التكافل التركيبة الجينية للتجمعات الجزائر المناطق ذات مناخ بيئيIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
The symbiosis Rhizobium-legume leads to the formation of root nodules fixing atmospheric nitrogen. This natural process has a considerable economic and agronomic interest. In Algeria, the production of food and forage legume is affected by environmental and climatic stresses (high temperature, water deficit...). In this study, total of 237 isolates were isolated from root nodules collected on lentil (Lens culinaris), proteaginous and forage peas (Pisum sativum) grown in the field in six sites representing two contrasting eco-climatic zones, sub humid and semi-arid in Eastern Algeria. The main objective was to determine the
degree of variability of the genetic structure of populations of Rhizobium nodulating among sites and isolated plants in different climatic conditions. Our isolates were characterized by PCR-restriction fragment length polymorphism (RFLP) of the 16S-23S rRNA intergenic region (IGS), and the nodD-F symbiotic region. The combination of these haplotypes allowed to clustered the isolates into 26 distinct genotypes. Phylogenetic analysis of 16S rDNA sequences allowed us to classify all isolates as Rhizobium leguminosarum. Results obtained showed that the variation of symbiotic marker (nodD-F) is low with the predominance of one
haplotype nod g, previously recovered at high frequency in Europe. Sequence analysis of IGS further confirmed its high variability in the studied strains. An AMOVA analysis showed highly significant differentiation in the IGS haplotype distribution between populations from both eco-climatic zones. This difference was reflected by differences in dominant genotype frequencies. Conversely we could not detect any host plant effect. The nodD gene sequence based phylogeny suggests that symbiotic gene diversity in pea and lentil nodulating rhizobial populations in Algeria is low compared to that reported elsewhere in the world.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/RIA6607.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9720 Diversité et structure génétique des populations de Rhizobium leguminosarum symbiovar viciae isolées du pois (Pisum sativum) et de la lentille (Lens culinaris) cultivés dans deux zones éco-climatiques subhumide et semi-aride de l'est Algérien [texte imprimé] / Nassira Riah, Auteur ; Abdelhamid Djekoun, Directeur de thèse ; Gisèle Laguerre, Directeur de thèse ; Philippe De Lajudie, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 131 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Pois lentille nodosités Rhizobium diversité symbiose structure génétique des
populations Algérie zones éco-climatiques Pea lentil nodules diversity symbiosis genetic structure of populations Algeria eco-climatic zones البازلا العدس العقد الجذرية الريزوبيوم التنوع التكافل التركيبة الجينية للتجمعات الجزائر المناطق ذات مناخ بيئيIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
The symbiosis Rhizobium-legume leads to the formation of root nodules fixing atmospheric nitrogen. This natural process has a considerable economic and agronomic interest. In Algeria, the production of food and forage legume is affected by environmental and climatic stresses (high temperature, water deficit...). In this study, total of 237 isolates were isolated from root nodules collected on lentil (Lens culinaris), proteaginous and forage peas (Pisum sativum) grown in the field in six sites representing two contrasting eco-climatic zones, sub humid and semi-arid in Eastern Algeria. The main objective was to determine the
degree of variability of the genetic structure of populations of Rhizobium nodulating among sites and isolated plants in different climatic conditions. Our isolates were characterized by PCR-restriction fragment length polymorphism (RFLP) of the 16S-23S rRNA intergenic region (IGS), and the nodD-F symbiotic region. The combination of these haplotypes allowed to clustered the isolates into 26 distinct genotypes. Phylogenetic analysis of 16S rDNA sequences allowed us to classify all isolates as Rhizobium leguminosarum. Results obtained showed that the variation of symbiotic marker (nodD-F) is low with the predominance of one
haplotype nod g, previously recovered at high frequency in Europe. Sequence analysis of IGS further confirmed its high variability in the studied strains. An AMOVA analysis showed highly significant differentiation in the IGS haplotype distribution between populations from both eco-climatic zones. This difference was reflected by differences in dominant genotype frequencies. Conversely we could not detect any host plant effect. The nodD gene sequence based phylogeny suggests that symbiotic gene diversity in pea and lentil nodulating rhizobial populations in Algeria is low compared to that reported elsewhere in the world.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/RIA6607.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9720 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité RIA/6607 RIA/6607 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intégreAdobe Acrobat PDF
Titre : Etude de la symbiose rhizobium pois chiche (Cicer arietinum L. : essai de production de l'inoculum et inoculation au champ Type de document : texte imprimé Auteurs : Univ. de Constantine, Éditeur scientifique Année de publication : 2001 Importance : 104 f. Note générale : 01 Disponible dans la salle de recherche
02 Disponibles au magasin de la B.U.C.Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Inoculum Rhizobium Symbiose Inoculation Cicer arietinum L. Index. décimale : 570 Sciences de la vie. Biologie Permalink : index.php?lvl=notice_display&id=1394 Etude de la symbiose rhizobium pois chiche (Cicer arietinum L. : essai de production de l'inoculum et inoculation au champ [texte imprimé] / Univ. de Constantine, Éditeur scientifique . - 2001 . - 104 f.
01 Disponible dans la salle de recherche
02 Disponibles au magasin de la B.U.C.
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Inoculum Rhizobium Symbiose Inoculation Cicer arietinum L. Index. décimale : 570 Sciences de la vie. Biologie Permalink : index.php?lvl=notice_display&id=1394 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité DEK/3568 DEK/3568 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude de la symbiose rhizobium - pois fourrager (pisum arvense L.) : essai de production d'inoculm et inoculation au champ Type de document : texte imprimé Auteurs : Univ. de Constantine, Éditeur scientifique Année de publication : 2001 Importance : 123 f. Note générale : 02 disponibles au magasin de la bibliothèque centrale
01 disponible à la salle de rechercheLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Inoculum Rhizobium Pisum Symbiose Nodule Nodosité Inoculation Index. décimale : 570 Sciences de la vie. Biologie Permalink : index.php?lvl=notice_display&id=1446 Etude de la symbiose rhizobium - pois fourrager (pisum arvense L.) : essai de production d'inoculm et inoculation au champ [texte imprimé] / Univ. de Constantine, Éditeur scientifique . - 2001 . - 123 f.
02 disponibles au magasin de la bibliothèque centrale
01 disponible à la salle de recherche
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Inoculum Rhizobium Pisum Symbiose Nodule Nodosité Inoculation Index. décimale : 570 Sciences de la vie. Biologie Permalink : index.php?lvl=notice_display&id=1446 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité SEM/3630 SEM/3630 Thèse Bibliothèque principale Thèses Disponible
Titre : Caractérisation des bactéries isolées à partir du genre Trigonella L. (Légumineuses) possant dans différents écosystèmes de l'Est algérien Type de document : texte imprimé Auteurs : Rabah Chabbi, Auteur ; Univ. de Constantine, Éditeur scientifique ; Yacine Benhizia, Directeur de thèse Année de publication : 2009 Importance : 103 f. Note générale : 01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Caractérisation Rhizobium Gammaproteobacteria Symbiote Trrigonella Characterization Symbiots Trogonella L وصف متعايش Index. décimale : 570 Sciences de la vie. Biologie Résumé : This work has been realized in order to evaluation and caracterisation of the
phenotypical diversity that exists within a collection of symbiots isolated from nodosities of
three Fenugreek populations originated form different regions of east Algeria.
The SDS page has revealed a great level of proteic polymorphism between the isolates. This
technique has allowed us to highlight the possible multiple infection among Trigonella L.
All the strains are infective towards Trigonella L with a relative efficiency that is
reflected by a good growing level of the obtained feet.
The fast growth of the strains and the assimilation of the charred substratum have
revealed profiles that are comparable to those of Rhizobium. In contrast, the strains have
manifested, like the Gammaproteobacteria, a great resistance to heavy metals and antibiotics.
A great tolerance to NaCl, pH, and temperature variation has been noticed for the whole
collection.
We have defined, on the base of the Azote fixative potential and the tolerance of
alkalinity, candidates that are efficient enough to be used for inoculation in the field.Diplôme : Magistère En ligne : ../theses/biologie/CHA5602.pdf Permalink : index.php?lvl=notice_display&id=1817 Caractérisation des bactéries isolées à partir du genre Trigonella L. (Légumineuses) possant dans différents écosystèmes de l'Est algérien [texte imprimé] / Rabah Chabbi, Auteur ; Univ. de Constantine, Éditeur scientifique ; Yacine Benhizia, Directeur de thèse . - 2009 . - 103 f.
01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Caractérisation Rhizobium Gammaproteobacteria Symbiote Trrigonella Characterization Symbiots Trogonella L وصف متعايش Index. décimale : 570 Sciences de la vie. Biologie Résumé : This work has been realized in order to evaluation and caracterisation of the
phenotypical diversity that exists within a collection of symbiots isolated from nodosities of
three Fenugreek populations originated form different regions of east Algeria.
The SDS page has revealed a great level of proteic polymorphism between the isolates. This
technique has allowed us to highlight the possible multiple infection among Trigonella L.
All the strains are infective towards Trigonella L with a relative efficiency that is
reflected by a good growing level of the obtained feet.
The fast growth of the strains and the assimilation of the charred substratum have
revealed profiles that are comparable to those of Rhizobium. In contrast, the strains have
manifested, like the Gammaproteobacteria, a great resistance to heavy metals and antibiotics.
A great tolerance to NaCl, pH, and temperature variation has been noticed for the whole
collection.
We have defined, on the base of the Azote fixative potential and the tolerance of
alkalinity, candidates that are efficient enough to be used for inoculation in the field.Diplôme : Magistère En ligne : ../theses/biologie/CHA5602.pdf Permalink : index.php?lvl=notice_display&id=1817 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité CHA/5602 CHA/5602 Thèse Bibliothèque principale Thèses Disponible
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