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Partager le résultat de cette recherche Interroger des sources externesBiodiversité de l’Orthoptérofaune (Criquets et Sauterelles) de la région de Sétif et étude de quelques aspects chimique et génétique / Zina Sofrane
Titre : Biodiversité de l’Orthoptérofaune (Criquets et Sauterelles) de la région de Sétif et étude de quelques aspects chimique et génétique Type de document : texte imprimé Auteurs : Zina Sofrane, Auteur ; Salaheddine Doumandji, Directeur de thèse ; Anne Geneviève Bagnères, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 134 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Diversité Caelifera Ensifera chémotaxonomie barcode phylogénie Diversity grasshoppers Katydids chemotaxonomy DNA barcoding Phylogeny التنوع البیولوجي Caeliferaالتصنیف الكیمیائي تحلیل الحمض النووي النسالة Index. décimale : 570 Sciences de la vie. Biologie Résumé : In the context of studying the biodiversity of orthopterfauna (Grasshoppers and Katydids) of the region of Setif, an inventory was conducted in three different ecosystems to determine the different species in the region. The diversity and abundance of this group of insects were evaluated between 2007 and 2009 in a fallow land, a reforestation of Aleppo pine and a wetland. Altogether 44 species were identified with 35 species of Caelifera and 9 species Ensifera (Katydids) distributed between 26 genus and 5 families. The Acrididae is widely predominated including 6 subfamilies. 13 new species are added to the region of Setif.
Integrated taxonomic methods, morphological, chemical and genetic were applied to 8 acridid species including Calliptamus barbarus , C.wattenwylianus, Oedipoda miniata, Acrotylus patruelis, A. insibricus, Sphingonotus maroccanus, Pseudosphingonotus finotianus, Sphingoderus carinatus . The results revealed for every species his various components of cuticular hydrocarbons and his DNA sequence. New taxonomic criteria are retained for
certain species and supplemented for others.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/SOF6942.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10319 Biodiversité de l’Orthoptérofaune (Criquets et Sauterelles) de la région de Sétif et étude de quelques aspects chimique et génétique [texte imprimé] / Zina Sofrane, Auteur ; Salaheddine Doumandji, Directeur de thèse ; Anne Geneviève Bagnères, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 134 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Diversité Caelifera Ensifera chémotaxonomie barcode phylogénie Diversity grasshoppers Katydids chemotaxonomy DNA barcoding Phylogeny التنوع البیولوجي Caeliferaالتصنیف الكیمیائي تحلیل الحمض النووي النسالة Index. décimale : 570 Sciences de la vie. Biologie Résumé : In the context of studying the biodiversity of orthopterfauna (Grasshoppers and Katydids) of the region of Setif, an inventory was conducted in three different ecosystems to determine the different species in the region. The diversity and abundance of this group of insects were evaluated between 2007 and 2009 in a fallow land, a reforestation of Aleppo pine and a wetland. Altogether 44 species were identified with 35 species of Caelifera and 9 species Ensifera (Katydids) distributed between 26 genus and 5 families. The Acrididae is widely predominated including 6 subfamilies. 13 new species are added to the region of Setif.
Integrated taxonomic methods, morphological, chemical and genetic were applied to 8 acridid species including Calliptamus barbarus , C.wattenwylianus, Oedipoda miniata, Acrotylus patruelis, A. insibricus, Sphingonotus maroccanus, Pseudosphingonotus finotianus, Sphingoderus carinatus . The results revealed for every species his various components of cuticular hydrocarbons and his DNA sequence. New taxonomic criteria are retained for
certain species and supplemented for others.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/SOF6942.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10319 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité SOF/6942 SOF/6942 Thèse Bibliothèque principale Thèses Disponible
Titre : Contribution à la connaissance des abeilles sauvages (Hymenoptera; Apoidea) dans les milieux naturels et cultivés de la région de Khenchela. Type de document : texte imprimé Auteurs : Noudjoud Maghni, Auteur ; K. Louadi, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2006 Importance : 139 f. Format : 30 cm. Note générale : 01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Entomologie:Application agronomique et médicale Hymenoptera Apoidea Abeilles sauvages Khenchela diversité nouvelle couverture végétale diversity new species vegetable cover خنشلة تنوع انواع جديدة الغطاء النباتي Index. décimale : 570 Sciences de la vie. Biologie Résumé :
This work consists of studying the pollinating Apoidea in the area of Khenchela in natural and cultivated environments. During the period between November 25, 2004 to October 27, 2005, we led an inventory of the species and their distribution in various altitudes. The highest peak is that of Chelia “2326 m.”, the two other stations: Tamza and Touchnet are at lower altitudes. At the end of this work 22 genera, 39 sub-genera and 80 species distributed between 5 families were listed on the level of the area of Khenchela. This study made it possible to retrain 15 new species and 3 subspecies for the fauna of Apoidea of Algeria. They were not announced by the authors of the beginning of
the 20th century, nor by the authors who worked recently on Apoidea of this country. Concerning the number of species for each family, we distinguished that Apidae mark the greatest number with 27 species followed by Halictidae (21 species), Megachilidae (16 species), Andrenidae (14 species) and finally Melittidae with 2 species.
The study of the composition of the fauna of Apoidea savages and the use of the ecological indexes of composition and structure made it possible to judge the structure of the populations of bees in the studied region. We showed that the distribution of the wild bees seems to depend on the type of the vegetable cover. These results prove the need for taking into account the conservation of the wild bees which play a dominating role in the pollination of the crop and the
spontaneous plants. The diversity of wild Apoidea appears primarily related to the diversity of the plants.Note de contenu : Annexes. Diplôme : Magistère En ligne : ../theses/biologie/MAG4684.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1355 Contribution à la connaissance des abeilles sauvages (Hymenoptera; Apoidea) dans les milieux naturels et cultivés de la région de Khenchela. [texte imprimé] / Noudjoud Maghni, Auteur ; K. Louadi, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2006 . - 139 f. ; 30 cm.
01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Entomologie:Application agronomique et médicale Hymenoptera Apoidea Abeilles sauvages Khenchela diversité nouvelle couverture végétale diversity new species vegetable cover خنشلة تنوع انواع جديدة الغطاء النباتي Index. décimale : 570 Sciences de la vie. Biologie Résumé :
This work consists of studying the pollinating Apoidea in the area of Khenchela in natural and cultivated environments. During the period between November 25, 2004 to October 27, 2005, we led an inventory of the species and their distribution in various altitudes. The highest peak is that of Chelia “2326 m.”, the two other stations: Tamza and Touchnet are at lower altitudes. At the end of this work 22 genera, 39 sub-genera and 80 species distributed between 5 families were listed on the level of the area of Khenchela. This study made it possible to retrain 15 new species and 3 subspecies for the fauna of Apoidea of Algeria. They were not announced by the authors of the beginning of
the 20th century, nor by the authors who worked recently on Apoidea of this country. Concerning the number of species for each family, we distinguished that Apidae mark the greatest number with 27 species followed by Halictidae (21 species), Megachilidae (16 species), Andrenidae (14 species) and finally Melittidae with 2 species.
The study of the composition of the fauna of Apoidea savages and the use of the ecological indexes of composition and structure made it possible to judge the structure of the populations of bees in the studied region. We showed that the distribution of the wild bees seems to depend on the type of the vegetable cover. These results prove the need for taking into account the conservation of the wild bees which play a dominating role in the pollination of the crop and the
spontaneous plants. The diversity of wild Apoidea appears primarily related to the diversity of the plants.Note de contenu : Annexes. Diplôme : Magistère En ligne : ../theses/biologie/MAG4684.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1355 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MAG/4684 MAG/4684 Thèse Bibliothèque principale Thèses Disponible 
Titre : Diversité génétique des céréales,recherche et caractérisation de leurs allergénes par l'approche protéomique Type de document : texte imprimé Auteurs : Ines Bellil, Auteur ; Douadi Khelifi, Directeur de thèse ; Sandra Denery, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2012 Importance : 211 f. Format : 30 cm Note générale : Doctorat 3ème cycle
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Blé Triticale Diversité Protéines Electrophorèses Allergies au blé Anticorps IgE Allergènes du blé Immunoblotting ELISA Analyse protéomique Wheat Diversity Proteins Electrophoresis Wheat allergy IgE antibodies Wheat allergens Immunoblitting Proteomic analysis القمح؛التنوع؛ بروتينات؛الهجرة الكهربائية للبروتينات؛حساسية للقمح؛المضادات الجسمية للمرضى من نوع ؛"IgE"بروتينات القمح مسببة الحساسية؛التحليل البروتيومي؛ Index. décimale : 570 Sciences de la vie. Biologie Résumé : In the first part of this study, two collections of wheat and one collection of triticale cultivated are analyzed to assess the genetic polymorphism of HMW-GS and SG-FPM: a collection of 69 triticale grown in France, another collection of 856 accessions of local durum wheat collected in Algeria and a priori divided into 17 botanical varieties according to their morphological characteristics, and the last two collections of 40 and 30 varieties of bread and durum wheats from Algerian oases. Biochemical analysis was performed by SDS-PAGE. In hexaploid triticale collection, allele nomenclature was compiled with a total of 36 alleles expressed at seven loci Glu-A1, Glu-B1, Glu-R1, Gli-R2, B2-Glu, Glu-A3, Glu- B3. This diversity has highlighted two major groups of triticale: winter and spring triticales.
Important variation was found in the collection of botanic durum wheat. Among the 16 alleles identified at the Glu-1 loci, two were new. The first named Glu-B1i1 encoding for two bands located between 17 and 18 which was assigned the nomenclature 171 and 181. The other named Glu-B1e1 codes two bands similar to 20x and 20y but with faster mobility, which were named 20x1-20y1. At Glu-3 and Glu-2 loci, 19 alleles were identified, where the allele named Glu-B3ab (encoding for subunits 2-8-9-13-16) was considered as new.
Analysis of Saharan wheats has identified a total of 32 and 29alleles for bread and durum wheat, respectively, which in combination resulted in 36 and 29 different patterns respectively. In bread wheat 3, 9 (including 1 new) and 4 alleles were observed at Glu-A1, Glu-B1 and Glu-D1 loci encoding HMW-GS respectively. LMW-GS displayed similar polymorphism, as 4, 9 and 3 alleles were identified at loci Glu-A3, Glu-B3 and Glu-D3 respectively. In durum wheat collection, 3 alleles were expressed at Glu-A1 locus and 9 (including 1 new) at Glu-B1. At Glu-A3, Glu-B3 and Glu-B2 loci 7, 8 (including 1 new) and 2 alleles were identified. Knowledge of these alleles is an important one hand to estimate the genetic variability and varietal identification and secondly in the selection for grain quality.
Another part of this study aimed to characterize the major allergens of wheat and / or cereals, to break down the specific IgE response to evaluate the role of different protein families in the allergenicity of wheat. The analysis included 10 varieties and 14 sera from allergic Algerian and French patients to wheat. All biochemical and immunological analysis showed that all proteins are recognized by patients regardless of clinical grade observed. 90% of the sera reacted fraction to albumins / globulins fraction in immunoblotting. IgE sera recognize more than one wheat protein and results can be observed that the immunoreactivity varies with genotypes. And the antigenic profile that emerges from these results is composed of five major antigens, albumins / globulins, /, and gliadins and glutenins subunits low molecular weight and a minor antigen subunits of glutenin high molecular weight. It is difficult to conclude on the type of sensitization to one or more common epitopes of these patients. Four protein bands were identified by proteomic analysis. Confronted by mass spectrometry analysis LC-MS/MS, we can suggest the existence of thirteen wheat allergens in the four groups analyzed.
Diplôme : Doctorat En ligne : ../theses/biologie/BEL6405.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9422 Diversité génétique des céréales,recherche et caractérisation de leurs allergénes par l'approche protéomique [texte imprimé] / Ines Bellil, Auteur ; Douadi Khelifi, Directeur de thèse ; Sandra Denery, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2012 . - 211 f. ; 30 cm.
Doctorat 3ème cycle
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Blé Triticale Diversité Protéines Electrophorèses Allergies au blé Anticorps IgE Allergènes du blé Immunoblotting ELISA Analyse protéomique Wheat Diversity Proteins Electrophoresis Wheat allergy IgE antibodies Wheat allergens Immunoblitting Proteomic analysis القمح؛التنوع؛ بروتينات؛الهجرة الكهربائية للبروتينات؛حساسية للقمح؛المضادات الجسمية للمرضى من نوع ؛"IgE"بروتينات القمح مسببة الحساسية؛التحليل البروتيومي؛ Index. décimale : 570 Sciences de la vie. Biologie Résumé : In the first part of this study, two collections of wheat and one collection of triticale cultivated are analyzed to assess the genetic polymorphism of HMW-GS and SG-FPM: a collection of 69 triticale grown in France, another collection of 856 accessions of local durum wheat collected in Algeria and a priori divided into 17 botanical varieties according to their morphological characteristics, and the last two collections of 40 and 30 varieties of bread and durum wheats from Algerian oases. Biochemical analysis was performed by SDS-PAGE. In hexaploid triticale collection, allele nomenclature was compiled with a total of 36 alleles expressed at seven loci Glu-A1, Glu-B1, Glu-R1, Gli-R2, B2-Glu, Glu-A3, Glu- B3. This diversity has highlighted two major groups of triticale: winter and spring triticales.
Important variation was found in the collection of botanic durum wheat. Among the 16 alleles identified at the Glu-1 loci, two were new. The first named Glu-B1i1 encoding for two bands located between 17 and 18 which was assigned the nomenclature 171 and 181. The other named Glu-B1e1 codes two bands similar to 20x and 20y but with faster mobility, which were named 20x1-20y1. At Glu-3 and Glu-2 loci, 19 alleles were identified, where the allele named Glu-B3ab (encoding for subunits 2-8-9-13-16) was considered as new.
Analysis of Saharan wheats has identified a total of 32 and 29alleles for bread and durum wheat, respectively, which in combination resulted in 36 and 29 different patterns respectively. In bread wheat 3, 9 (including 1 new) and 4 alleles were observed at Glu-A1, Glu-B1 and Glu-D1 loci encoding HMW-GS respectively. LMW-GS displayed similar polymorphism, as 4, 9 and 3 alleles were identified at loci Glu-A3, Glu-B3 and Glu-D3 respectively. In durum wheat collection, 3 alleles were expressed at Glu-A1 locus and 9 (including 1 new) at Glu-B1. At Glu-A3, Glu-B3 and Glu-B2 loci 7, 8 (including 1 new) and 2 alleles were identified. Knowledge of these alleles is an important one hand to estimate the genetic variability and varietal identification and secondly in the selection for grain quality.
Another part of this study aimed to characterize the major allergens of wheat and / or cereals, to break down the specific IgE response to evaluate the role of different protein families in the allergenicity of wheat. The analysis included 10 varieties and 14 sera from allergic Algerian and French patients to wheat. All biochemical and immunological analysis showed that all proteins are recognized by patients regardless of clinical grade observed. 90% of the sera reacted fraction to albumins / globulins fraction in immunoblotting. IgE sera recognize more than one wheat protein and results can be observed that the immunoreactivity varies with genotypes. And the antigenic profile that emerges from these results is composed of five major antigens, albumins / globulins, /, and gliadins and glutenins subunits low molecular weight and a minor antigen subunits of glutenin high molecular weight. It is difficult to conclude on the type of sensitization to one or more common epitopes of these patients. Four protein bands were identified by proteomic analysis. Confronted by mass spectrometry analysis LC-MS/MS, we can suggest the existence of thirteen wheat allergens in the four groups analyzed.
Diplôme : Doctorat En ligne : ../theses/biologie/BEL6405.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9422 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEL/6405 BEL/6405 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intègraleAdobe Acrobat PDF
Titre : Diversité génétique de l'espèce Aegilops geniculata Roth en Algérie par approches morphologique, biochimique et moléculaire Type de document : texte imprimé Auteurs : Asma Medouri, Auteur ; Douadi Khelifi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 132 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Aegilops geniculata Accession Algérie Diversité Morphologie Gluténines Gliadines RAPD Algeria Diversity Morphology Glutenins Gliadins الجزائر عينة التنوع الوراثي مورفولوجيا غلوتينين غليادين Index. décimale : 570 Sciences de la vie. Biologie Résumé : The study of genetic diversity in a collection of the allotetraploid species Aegilops geniculata Roth(UM) collected in Algeria was assessed using morphological, biochemical and molecular approaches.
This was carried on a wide sampling following a climatic gradient throughout North Algeria. This estimation was preceded by assessing the genetic variability of the diploid progenitors of the tetraploid species (Ae. comosa and Ae. umbellulata) based on gluten proteins. For diploids, a significant variation
was revealed for the three classes of gluten proteins. Several new alleles corresponding to HMW-GS were identified and a nomenclature was proposed as: 8 alleles were found at the Glu-M1 locus (with 5 new alleles) and 3 alleles (with one new) at the Glu-U1 locus. For LMW-GS (B- zone), 14 and 7 alleles were revealed at Glu-M3 and Glu-U3 loci respectively. For gliadins, a typology was established.
Morphological diversity of Ae. geniculata was estimated using 15 descriptors. Individuals were morphologically homogenous and little differences were found between accessions. Results of PCA reveal that traits expressing the wide of the spike distinguished better between accessions. Biochemical analysis of glutenins showed a high level of polymorphism as; 15 alleles were recognized at the GluM1 locus and 12 at the Glu-U1 locus with a new subunit and several new alleles. For B-LMW-GS,
the locus Glu-M3 expressed 28 alleles and the locus Glu-U3 expressed 25 alleles. Thirty-two specific patterns were revealed for C-LMW-GS. With respect to gliadins, 61 polymorphic bands resulting in 35 gliadin blocks were found. The genetic diversity index indicated that ω-gliadins were the most polymorphic, followed by γ- and β-gliadin then α-gliadins. Molecular data based on RAPD showed a high polymorphism as 145 fragments were detected with a level of polymorphism of 79.31%.
The three studied approaches (morphology, proteins and DNA polymorphism) showed relationships between genetic variation of the accessions and ecogeographic conditions. Correlation between morphology and some climatic factors showed that some independent characters were correlated with the winter minimum temperature. The polymorphism detected in gliadins highlighted a strong correlation between ecogeographic parameters and some ω-gliadin bands especially the band B14.
RAPD polymorphism showed a strong correlation with ecological parameters."
Diplôme : Doctorat En ligne : ../theses/biologie/MED6917.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10269 Diversité génétique de l'espèce Aegilops geniculata Roth en Algérie par approches morphologique, biochimique et moléculaire [texte imprimé] / Asma Medouri, Auteur ; Douadi Khelifi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 132 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Aegilops geniculata Accession Algérie Diversité Morphologie Gluténines Gliadines RAPD Algeria Diversity Morphology Glutenins Gliadins الجزائر عينة التنوع الوراثي مورفولوجيا غلوتينين غليادين Index. décimale : 570 Sciences de la vie. Biologie Résumé : The study of genetic diversity in a collection of the allotetraploid species Aegilops geniculata Roth(UM) collected in Algeria was assessed using morphological, biochemical and molecular approaches.
This was carried on a wide sampling following a climatic gradient throughout North Algeria. This estimation was preceded by assessing the genetic variability of the diploid progenitors of the tetraploid species (Ae. comosa and Ae. umbellulata) based on gluten proteins. For diploids, a significant variation
was revealed for the three classes of gluten proteins. Several new alleles corresponding to HMW-GS were identified and a nomenclature was proposed as: 8 alleles were found at the Glu-M1 locus (with 5 new alleles) and 3 alleles (with one new) at the Glu-U1 locus. For LMW-GS (B- zone), 14 and 7 alleles were revealed at Glu-M3 and Glu-U3 loci respectively. For gliadins, a typology was established.
Morphological diversity of Ae. geniculata was estimated using 15 descriptors. Individuals were morphologically homogenous and little differences were found between accessions. Results of PCA reveal that traits expressing the wide of the spike distinguished better between accessions. Biochemical analysis of glutenins showed a high level of polymorphism as; 15 alleles were recognized at the GluM1 locus and 12 at the Glu-U1 locus with a new subunit and several new alleles. For B-LMW-GS,
the locus Glu-M3 expressed 28 alleles and the locus Glu-U3 expressed 25 alleles. Thirty-two specific patterns were revealed for C-LMW-GS. With respect to gliadins, 61 polymorphic bands resulting in 35 gliadin blocks were found. The genetic diversity index indicated that ω-gliadins were the most polymorphic, followed by γ- and β-gliadin then α-gliadins. Molecular data based on RAPD showed a high polymorphism as 145 fragments were detected with a level of polymorphism of 79.31%.
The three studied approaches (morphology, proteins and DNA polymorphism) showed relationships between genetic variation of the accessions and ecogeographic conditions. Correlation between morphology and some climatic factors showed that some independent characters were correlated with the winter minimum temperature. The polymorphism detected in gliadins highlighted a strong correlation between ecogeographic parameters and some ω-gliadin bands especially the band B14.
RAPD polymorphism showed a strong correlation with ecological parameters."
Diplôme : Doctorat En ligne : ../theses/biologie/MED6917.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10269 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MED/6917 MED/6917 Thèse Bibliothèque principale Thèses Disponible
Titre : Diversité et structure génétique des populations de Rhizobium leguminosarum symbiovar viciae isolées du pois (Pisum sativum) et de la lentille (Lens culinaris) cultivés dans deux zones éco-climatiques subhumide et semi-aride de l'est Algérien Type de document : texte imprimé Auteurs : Nassira Riah, Auteur ; Abdelhamid Djekoun, Directeur de thèse ; Gisèle Laguerre, Directeur de thèse ; Philippe De Lajudie, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 131 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Pois lentille nodosités Rhizobium diversité symbiose structure génétique des
populations Algérie zones éco-climatiques Pea lentil nodules diversity symbiosis genetic structure of populations Algeria eco-climatic zones البازلا العدس العقد الجذرية الريزوبيوم التنوع التكافل التركيبة الجينية للتجمعات الجزائر المناطق ذات مناخ بيئيIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
The symbiosis Rhizobium-legume leads to the formation of root nodules fixing atmospheric nitrogen. This natural process has a considerable economic and agronomic interest. In Algeria, the production of food and forage legume is affected by environmental and climatic stresses (high temperature, water deficit...). In this study, total of 237 isolates were isolated from root nodules collected on lentil (Lens culinaris), proteaginous and forage peas (Pisum sativum) grown in the field in six sites representing two contrasting eco-climatic zones, sub humid and semi-arid in Eastern Algeria. The main objective was to determine the
degree of variability of the genetic structure of populations of Rhizobium nodulating among sites and isolated plants in different climatic conditions. Our isolates were characterized by PCR-restriction fragment length polymorphism (RFLP) of the 16S-23S rRNA intergenic region (IGS), and the nodD-F symbiotic region. The combination of these haplotypes allowed to clustered the isolates into 26 distinct genotypes. Phylogenetic analysis of 16S rDNA sequences allowed us to classify all isolates as Rhizobium leguminosarum. Results obtained showed that the variation of symbiotic marker (nodD-F) is low with the predominance of one
haplotype nod g, previously recovered at high frequency in Europe. Sequence analysis of IGS further confirmed its high variability in the studied strains. An AMOVA analysis showed highly significant differentiation in the IGS haplotype distribution between populations from both eco-climatic zones. This difference was reflected by differences in dominant genotype frequencies. Conversely we could not detect any host plant effect. The nodD gene sequence based phylogeny suggests that symbiotic gene diversity in pea and lentil nodulating rhizobial populations in Algeria is low compared to that reported elsewhere in the world.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/RIA6607.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9720 Diversité et structure génétique des populations de Rhizobium leguminosarum symbiovar viciae isolées du pois (Pisum sativum) et de la lentille (Lens culinaris) cultivés dans deux zones éco-climatiques subhumide et semi-aride de l'est Algérien [texte imprimé] / Nassira Riah, Auteur ; Abdelhamid Djekoun, Directeur de thèse ; Gisèle Laguerre, Directeur de thèse ; Philippe De Lajudie, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 131 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Pois lentille nodosités Rhizobium diversité symbiose structure génétique des
populations Algérie zones éco-climatiques Pea lentil nodules diversity symbiosis genetic structure of populations Algeria eco-climatic zones البازلا العدس العقد الجذرية الريزوبيوم التنوع التكافل التركيبة الجينية للتجمعات الجزائر المناطق ذات مناخ بيئيIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
The symbiosis Rhizobium-legume leads to the formation of root nodules fixing atmospheric nitrogen. This natural process has a considerable economic and agronomic interest. In Algeria, the production of food and forage legume is affected by environmental and climatic stresses (high temperature, water deficit...). In this study, total of 237 isolates were isolated from root nodules collected on lentil (Lens culinaris), proteaginous and forage peas (Pisum sativum) grown in the field in six sites representing two contrasting eco-climatic zones, sub humid and semi-arid in Eastern Algeria. The main objective was to determine the
degree of variability of the genetic structure of populations of Rhizobium nodulating among sites and isolated plants in different climatic conditions. Our isolates were characterized by PCR-restriction fragment length polymorphism (RFLP) of the 16S-23S rRNA intergenic region (IGS), and the nodD-F symbiotic region. The combination of these haplotypes allowed to clustered the isolates into 26 distinct genotypes. Phylogenetic analysis of 16S rDNA sequences allowed us to classify all isolates as Rhizobium leguminosarum. Results obtained showed that the variation of symbiotic marker (nodD-F) is low with the predominance of one
haplotype nod g, previously recovered at high frequency in Europe. Sequence analysis of IGS further confirmed its high variability in the studied strains. An AMOVA analysis showed highly significant differentiation in the IGS haplotype distribution between populations from both eco-climatic zones. This difference was reflected by differences in dominant genotype frequencies. Conversely we could not detect any host plant effect. The nodD gene sequence based phylogeny suggests that symbiotic gene diversity in pea and lentil nodulating rhizobial populations in Algeria is low compared to that reported elsewhere in the world.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/RIA6607.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9720 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité RIA/6607 RIA/6607 Thèse Bibliothèque principale Thèses Disponible Documents numériques
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