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Partager le résultat de cette recherche Interroger des sources externesDiversité génétique de l'espèce Aegilops geniculata Roth en Algérie par approches morphologique, biochimique et moléculaire / Asma Medouri
Titre : Diversité génétique de l'espèce Aegilops geniculata Roth en Algérie par approches morphologique, biochimique et moléculaire Type de document : texte imprimé Auteurs : Asma Medouri, Auteur ; Douadi Khelifi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 132 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Aegilops geniculata Accession Algérie Diversité Morphologie Gluténines Gliadines RAPD Algeria Diversity Morphology Glutenins Gliadins الجزائر عينة التنوع الوراثي مورفولوجيا غلوتينين غليادين Index. décimale : 570 Sciences de la vie. Biologie Résumé : The study of genetic diversity in a collection of the allotetraploid species Aegilops geniculata Roth(UM) collected in Algeria was assessed using morphological, biochemical and molecular approaches.
This was carried on a wide sampling following a climatic gradient throughout North Algeria. This estimation was preceded by assessing the genetic variability of the diploid progenitors of the tetraploid species (Ae. comosa and Ae. umbellulata) based on gluten proteins. For diploids, a significant variation
was revealed for the three classes of gluten proteins. Several new alleles corresponding to HMW-GS were identified and a nomenclature was proposed as: 8 alleles were found at the Glu-M1 locus (with 5 new alleles) and 3 alleles (with one new) at the Glu-U1 locus. For LMW-GS (B- zone), 14 and 7 alleles were revealed at Glu-M3 and Glu-U3 loci respectively. For gliadins, a typology was established.
Morphological diversity of Ae. geniculata was estimated using 15 descriptors. Individuals were morphologically homogenous and little differences were found between accessions. Results of PCA reveal that traits expressing the wide of the spike distinguished better between accessions. Biochemical analysis of glutenins showed a high level of polymorphism as; 15 alleles were recognized at the GluM1 locus and 12 at the Glu-U1 locus with a new subunit and several new alleles. For B-LMW-GS,
the locus Glu-M3 expressed 28 alleles and the locus Glu-U3 expressed 25 alleles. Thirty-two specific patterns were revealed for C-LMW-GS. With respect to gliadins, 61 polymorphic bands resulting in 35 gliadin blocks were found. The genetic diversity index indicated that ω-gliadins were the most polymorphic, followed by γ- and β-gliadin then α-gliadins. Molecular data based on RAPD showed a high polymorphism as 145 fragments were detected with a level of polymorphism of 79.31%.
The three studied approaches (morphology, proteins and DNA polymorphism) showed relationships between genetic variation of the accessions and ecogeographic conditions. Correlation between morphology and some climatic factors showed that some independent characters were correlated with the winter minimum temperature. The polymorphism detected in gliadins highlighted a strong correlation between ecogeographic parameters and some ω-gliadin bands especially the band B14.
RAPD polymorphism showed a strong correlation with ecological parameters."
Diplôme : Doctorat En ligne : ../theses/biologie/MED6917.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10269 Diversité génétique de l'espèce Aegilops geniculata Roth en Algérie par approches morphologique, biochimique et moléculaire [texte imprimé] / Asma Medouri, Auteur ; Douadi Khelifi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 132 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Aegilops geniculata Accession Algérie Diversité Morphologie Gluténines Gliadines RAPD Algeria Diversity Morphology Glutenins Gliadins الجزائر عينة التنوع الوراثي مورفولوجيا غلوتينين غليادين Index. décimale : 570 Sciences de la vie. Biologie Résumé : The study of genetic diversity in a collection of the allotetraploid species Aegilops geniculata Roth(UM) collected in Algeria was assessed using morphological, biochemical and molecular approaches.
This was carried on a wide sampling following a climatic gradient throughout North Algeria. This estimation was preceded by assessing the genetic variability of the diploid progenitors of the tetraploid species (Ae. comosa and Ae. umbellulata) based on gluten proteins. For diploids, a significant variation
was revealed for the three classes of gluten proteins. Several new alleles corresponding to HMW-GS were identified and a nomenclature was proposed as: 8 alleles were found at the Glu-M1 locus (with 5 new alleles) and 3 alleles (with one new) at the Glu-U1 locus. For LMW-GS (B- zone), 14 and 7 alleles were revealed at Glu-M3 and Glu-U3 loci respectively. For gliadins, a typology was established.
Morphological diversity of Ae. geniculata was estimated using 15 descriptors. Individuals were morphologically homogenous and little differences were found between accessions. Results of PCA reveal that traits expressing the wide of the spike distinguished better between accessions. Biochemical analysis of glutenins showed a high level of polymorphism as; 15 alleles were recognized at the GluM1 locus and 12 at the Glu-U1 locus with a new subunit and several new alleles. For B-LMW-GS,
the locus Glu-M3 expressed 28 alleles and the locus Glu-U3 expressed 25 alleles. Thirty-two specific patterns were revealed for C-LMW-GS. With respect to gliadins, 61 polymorphic bands resulting in 35 gliadin blocks were found. The genetic diversity index indicated that ω-gliadins were the most polymorphic, followed by γ- and β-gliadin then α-gliadins. Molecular data based on RAPD showed a high polymorphism as 145 fragments were detected with a level of polymorphism of 79.31%.
The three studied approaches (morphology, proteins and DNA polymorphism) showed relationships between genetic variation of the accessions and ecogeographic conditions. Correlation between morphology and some climatic factors showed that some independent characters were correlated with the winter minimum temperature. The polymorphism detected in gliadins highlighted a strong correlation between ecogeographic parameters and some ω-gliadin bands especially the band B14.
RAPD polymorphism showed a strong correlation with ecological parameters."
Diplôme : Doctorat En ligne : ../theses/biologie/MED6917.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10269 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MED/6917 MED/6917 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude moléculaire du polymorphisme des protéines de réserve d’une accession de blé dur (Triticum durum Desf.) Cultivé en Algérie. Type de document : texte imprimé Auteurs : Nabila Adoui, Auteur ; Leila Boudour, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 164 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie et Ecologie Végétale: Biologie végétale Biologie végétale : Biodiversité et Production Végétale Blé dur polymorphisme gènes codant gluténines gliadines diversité morphologique marqueurs moléculaires RFLP séquençage durum wheat polymorphism coding genes glutenins gliadins morphological
diversity molecular markers RELF sequencing القمح الصلب التعدد الشكلي جینات الترمیز الغلوتینات الجلیادین التنوع
المورفولوجي الموسمات الجزیئیة RFLPالتسلسلIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
The managment and conservation of genetic ressources of a cultivated specie must requir a good knowledge of its morphological and genetic variability for its conservation against its erosion. For this we have carried out an agro-morphological, biochemical and molecular study, in order to appreciate the morphological and agronomical diversity intra and inter-varietal, as well as the polymorphism biochemical and genetic of reservation’s protein (glutenins HPM, FRM and the gliadins) existing among the 100 genotypes belonging the four local varieties of durum wheat (Triticum durum Desf .) ,namely, alexandrinum, merciense, valenciae and italicum cultivated in Algeria. The measurments of agro-morphological parameters were carried out at plants maturity. The results obtained by of agro-morphological parameters showed the existence of a very important inter-varietal diversity between the four varieties so that the alexandrinum and italicum variety’s genotypes have the highest performances values, while murciense variety’s genotypes show the lowest values. The biochemical analyse by one-dimentional electophoresis (SDS-PAGE) revealed the presence of low intra-varietal polymorphism and important polymorphism between the genotypes of four varietes ; the genotypes of alexandrinum and murceinse variety have - - gliadins bands, while the gemotypes of valenciae variety present type et gliadins bands. Molecular study include three technical’s type , namely PCR/BULK technical, molecular marking across the PCR/RELF marker and genes’s sequencing coding gliadins. The two primers GLUDX5 and LA1 aimed at the amplification of glutenins’s genes highliting the the poverty of these genes in all genotypes of the four varieties. While the primer 56MN used for the amplification of gliadins’s genes wich shows the abudance of these in all the genotypes of four varieties. For the marker PCR/RELF apply on eleven genotypes of four varieties slected on the basis of thier abudance of gliadins’s genes produced the same namber amplifing molecular wheight 536 pb. With eleven monomorphic fragments. The sequencing’s analyse of two genotypes selected of any variety shows the existence of a very weak polymorphism between the genotypes of four varieties and reveal that they are very genetically related.Note de contenu :
Annexes.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ADO7428.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11082 Etude moléculaire du polymorphisme des protéines de réserve d’une accession de blé dur (Triticum durum Desf.) Cultivé en Algérie. [texte imprimé] / Nabila Adoui, Auteur ; Leila Boudour, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 164 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie et Ecologie Végétale: Biologie végétale Biologie végétale : Biodiversité et Production Végétale Blé dur polymorphisme gènes codant gluténines gliadines diversité morphologique marqueurs moléculaires RFLP séquençage durum wheat polymorphism coding genes glutenins gliadins morphological
diversity molecular markers RELF sequencing القمح الصلب التعدد الشكلي جینات الترمیز الغلوتینات الجلیادین التنوع
المورفولوجي الموسمات الجزیئیة RFLPالتسلسلIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
The managment and conservation of genetic ressources of a cultivated specie must requir a good knowledge of its morphological and genetic variability for its conservation against its erosion. For this we have carried out an agro-morphological, biochemical and molecular study, in order to appreciate the morphological and agronomical diversity intra and inter-varietal, as well as the polymorphism biochemical and genetic of reservation’s protein (glutenins HPM, FRM and the gliadins) existing among the 100 genotypes belonging the four local varieties of durum wheat (Triticum durum Desf .) ,namely, alexandrinum, merciense, valenciae and italicum cultivated in Algeria. The measurments of agro-morphological parameters were carried out at plants maturity. The results obtained by of agro-morphological parameters showed the existence of a very important inter-varietal diversity between the four varieties so that the alexandrinum and italicum variety’s genotypes have the highest performances values, while murciense variety’s genotypes show the lowest values. The biochemical analyse by one-dimentional electophoresis (SDS-PAGE) revealed the presence of low intra-varietal polymorphism and important polymorphism between the genotypes of four varietes ; the genotypes of alexandrinum and murceinse variety have - - gliadins bands, while the gemotypes of valenciae variety present type et gliadins bands. Molecular study include three technical’s type , namely PCR/BULK technical, molecular marking across the PCR/RELF marker and genes’s sequencing coding gliadins. The two primers GLUDX5 and LA1 aimed at the amplification of glutenins’s genes highliting the the poverty of these genes in all genotypes of the four varieties. While the primer 56MN used for the amplification of gliadins’s genes wich shows the abudance of these in all the genotypes of four varieties. For the marker PCR/RELF apply on eleven genotypes of four varieties slected on the basis of thier abudance of gliadins’s genes produced the same namber amplifing molecular wheight 536 pb. With eleven monomorphic fragments. The sequencing’s analyse of two genotypes selected of any variety shows the existence of a very weak polymorphism between the genotypes of four varieties and reveal that they are very genetically related.Note de contenu :
Annexes.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ADO7428.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11082 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ADO/7428 ADO/7428 Thèse Bibliothèque principale Thèses Disponible
Titre : Utilisation des marqueurs biochimiques dans l'amélioration génétique de la qualité des blés Type de document : texte imprimé Auteurs : Univ. de Constantine, Éditeur scientifique Année de publication : 2001 Importance : 82 f. Note générale : 1 disponible dans la salle de recherche
2 disponibles au magasin de la bibliothèque centraleLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Blé dur Blé tendre Gliadines gluténines allèle Electrophorèse Diversité génétique qualité Index. décimale : 570 Sciences de la vie. Biologie Permalink : index.php?lvl=notice_display&id=1733 Utilisation des marqueurs biochimiques dans l'amélioration génétique de la qualité des blés [texte imprimé] / Univ. de Constantine, Éditeur scientifique . - 2001 . - 82 f.
1 disponible dans la salle de recherche
2 disponibles au magasin de la bibliothèque centrale
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Blé dur Blé tendre Gliadines gluténines allèle Electrophorèse Diversité génétique qualité Index. décimale : 570 Sciences de la vie. Biologie Permalink : index.php?lvl=notice_display&id=1733 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEN/3535 BEN/3535 Thèse Bibliothèque principale Thèses Disponible
