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Partager le résultat de cette recherche Interroger des sources externesDiversité de 13 génotypes d'orge (Hordeum vulgare L.) et de 13 génotypes de blé tendre (Triticum aestivum L.)
Titre : Diversité de 13 génotypes d'orge (Hordeum vulgare L.) et de 13 génotypes de blé tendre (Triticum aestivum L.) : Etude des caractères de production et d'adaptation Type de document : texte imprimé Auteurs : Université Mentouri, Editeur scientifique Année de publication : 2009 Importance : 167 f. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Production Adaptation Morphologie Physiologie Diversité Triticum aestivum L. Hordeum vulgare L. Phénologie Génotype et éspèce Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/SOU5299.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1786 Diversité de 13 génotypes d'orge (Hordeum vulgare L.) et de 13 génotypes de blé tendre (Triticum aestivum L.) : Etude des caractères de production et d'adaptation [texte imprimé] / Université Mentouri, Editeur scientifique . - 2009 . - 167 f.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Production Adaptation Morphologie Physiologie Diversité Triticum aestivum L. Hordeum vulgare L. Phénologie Génotype et éspèce Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/SOU5299.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1786 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité SOU/5299 SOU/5299 Thèse Bibliothèque principale Thèses Disponible
Titre : Diversité du blé dur (Triticum durum Desf.) : étude des caractères de production et d'adaptation Type de document : texte imprimé Auteurs : Université Mentouri, Editeur scientifique Année de publication : 2010 Importance : 135 f. Note générale : 01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Adaptation Génotype Diversité Triticum durum Desf. Morpho-phénologie Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/OUD5244.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1810 Diversité du blé dur (Triticum durum Desf.) : étude des caractères de production et d'adaptation [texte imprimé] / Université Mentouri, Editeur scientifique . - 2010 . - 135 f.
01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Adaptation Génotype Diversité Triticum durum Desf. Morpho-phénologie Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/OUD5244.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1810 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité OUD/5244 OUD/5244 Thèse Bibliothèque principale Thèses Disponible 
Titre : Diversité génétique des céréales,recherche et caractérisation de leurs allergénes par l'approche protéomique Type de document : texte imprimé Auteurs : Ines Bellil, Auteur ; Douadi Khelifi, Directeur de thèse ; Sandra Denery, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2012 Importance : 211 f. Format : 30 cm Note générale : Doctorat 3ème cycle
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Blé Triticale Diversité Protéines Electrophorèses Allergies au blé Anticorps IgE Allergènes du blé Immunoblotting ELISA Analyse protéomique Wheat Diversity Proteins Electrophoresis Wheat allergy IgE antibodies Wheat allergens Immunoblitting Proteomic analysis ????????????? ??????????????? ?????????? ????????????????? ?????????????? ??????? ?????? ?? ??? ?"IgE"???????? ????? ????? ???????????????? ??????????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : In the first part of this study, two collections of wheat and one collection of triticale cultivated are analyzed to assess the genetic polymorphism of HMW-GS and SG-FPM: a collection of 69 triticale grown in France, another collection of 856 accessions of local durum wheat collected in Algeria and a priori divided into 17 botanical varieties according to their morphological characteristics, and the last two collections of 40 and 30 varieties of bread and durum wheats from Algerian oases. Biochemical analysis was performed by SDS-PAGE. In hexaploid triticale collection, allele nomenclature was compiled with a total of 36 alleles expressed at seven loci Glu-A1, Glu-B1, Glu-R1, Gli-R2, B2-Glu, Glu-A3, Glu- B3. This diversity has highlighted two major groups of triticale: winter and spring triticales.
Important variation was found in the collection of botanic durum wheat. Among the 16 alleles identified at the Glu-1 loci, two were new. The first named Glu-B1i1 encoding for two bands located between 17 and 18 which was assigned the nomenclature 171 and 181. The other named Glu-B1e1 codes two bands similar to 20x and 20y but with faster mobility, which were named 20x1-20y1. At Glu-3 and Glu-2 loci, 19 alleles were identified, where the allele named Glu-B3ab (encoding for subunits 2-8-9-13-16) was considered as new.
Analysis of Saharan wheats has identified a total of 32 and 29alleles for bread and durum wheat, respectively, which in combination resulted in 36 and 29 different patterns respectively. In bread wheat 3, 9 (including 1 new) and 4 alleles were observed at Glu-A1, Glu-B1 and Glu-D1 loci encoding HMW-GS respectively. LMW-GS displayed similar polymorphism, as 4, 9 and 3 alleles were identified at loci Glu-A3, Glu-B3 and Glu-D3 respectively. In durum wheat collection, 3 alleles were expressed at Glu-A1 locus and 9 (including 1 new) at Glu-B1. At Glu-A3, Glu-B3 and Glu-B2 loci 7, 8 (including 1 new) and 2 alleles were identified. Knowledge of these alleles is an important one hand to estimate the genetic variability and varietal identification and secondly in the selection for grain quality.
Another part of this study aimed to characterize the major allergens of wheat and / or cereals, to break down the specific IgE response to evaluate the role of different protein families in the allergenicity of wheat. The analysis included 10 varieties and 14 sera from allergic Algerian and French patients to wheat. All biochemical and immunological analysis showed that all proteins are recognized by patients regardless of clinical grade observed. 90% of the sera reacted fraction to albumins / globulins fraction in immunoblotting. IgE sera recognize more than one wheat protein and results can be observed that the immunoreactivity varies with genotypes. And the antigenic profile that emerges from these results is composed of five major antigens, albumins / globulins, /, and gliadins and glutenins subunits low molecular weight and a minor antigen subunits of glutenin high molecular weight. It is difficult to conclude on the type of sensitization to one or more common epitopes of these patients. Four protein bands were identified by proteomic analysis. Confronted by mass spectrometry analysis LC-MS/MS, we can suggest the existence of thirteen wheat allergens in the four groups analyzed.
Diplôme : Doctorat En ligne : ../theses/biologie/BEL6405.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9422 Diversité génétique des céréales,recherche et caractérisation de leurs allergénes par l'approche protéomique [texte imprimé] / Ines Bellil, Auteur ; Douadi Khelifi, Directeur de thèse ; Sandra Denery, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2012 . - 211 f. ; 30 cm.
Doctorat 3ème cycle
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Blé Triticale Diversité Protéines Electrophorèses Allergies au blé Anticorps IgE Allergènes du blé Immunoblotting ELISA Analyse protéomique Wheat Diversity Proteins Electrophoresis Wheat allergy IgE antibodies Wheat allergens Immunoblitting Proteomic analysis ????????????? ??????????????? ?????????? ????????????????? ?????????????? ??????? ?????? ?? ??? ?"IgE"???????? ????? ????? ???????????????? ??????????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : In the first part of this study, two collections of wheat and one collection of triticale cultivated are analyzed to assess the genetic polymorphism of HMW-GS and SG-FPM: a collection of 69 triticale grown in France, another collection of 856 accessions of local durum wheat collected in Algeria and a priori divided into 17 botanical varieties according to their morphological characteristics, and the last two collections of 40 and 30 varieties of bread and durum wheats from Algerian oases. Biochemical analysis was performed by SDS-PAGE. In hexaploid triticale collection, allele nomenclature was compiled with a total of 36 alleles expressed at seven loci Glu-A1, Glu-B1, Glu-R1, Gli-R2, B2-Glu, Glu-A3, Glu- B3. This diversity has highlighted two major groups of triticale: winter and spring triticales.
Important variation was found in the collection of botanic durum wheat. Among the 16 alleles identified at the Glu-1 loci, two were new. The first named Glu-B1i1 encoding for two bands located between 17 and 18 which was assigned the nomenclature 171 and 181. The other named Glu-B1e1 codes two bands similar to 20x and 20y but with faster mobility, which were named 20x1-20y1. At Glu-3 and Glu-2 loci, 19 alleles were identified, where the allele named Glu-B3ab (encoding for subunits 2-8-9-13-16) was considered as new.
Analysis of Saharan wheats has identified a total of 32 and 29alleles for bread and durum wheat, respectively, which in combination resulted in 36 and 29 different patterns respectively. In bread wheat 3, 9 (including 1 new) and 4 alleles were observed at Glu-A1, Glu-B1 and Glu-D1 loci encoding HMW-GS respectively. LMW-GS displayed similar polymorphism, as 4, 9 and 3 alleles were identified at loci Glu-A3, Glu-B3 and Glu-D3 respectively. In durum wheat collection, 3 alleles were expressed at Glu-A1 locus and 9 (including 1 new) at Glu-B1. At Glu-A3, Glu-B3 and Glu-B2 loci 7, 8 (including 1 new) and 2 alleles were identified. Knowledge of these alleles is an important one hand to estimate the genetic variability and varietal identification and secondly in the selection for grain quality.
Another part of this study aimed to characterize the major allergens of wheat and / or cereals, to break down the specific IgE response to evaluate the role of different protein families in the allergenicity of wheat. The analysis included 10 varieties and 14 sera from allergic Algerian and French patients to wheat. All biochemical and immunological analysis showed that all proteins are recognized by patients regardless of clinical grade observed. 90% of the sera reacted fraction to albumins / globulins fraction in immunoblotting. IgE sera recognize more than one wheat protein and results can be observed that the immunoreactivity varies with genotypes. And the antigenic profile that emerges from these results is composed of five major antigens, albumins / globulins, /, and gliadins and glutenins subunits low molecular weight and a minor antigen subunits of glutenin high molecular weight. It is difficult to conclude on the type of sensitization to one or more common epitopes of these patients. Four protein bands were identified by proteomic analysis. Confronted by mass spectrometry analysis LC-MS/MS, we can suggest the existence of thirteen wheat allergens in the four groups analyzed.
Diplôme : Doctorat En ligne : ../theses/biologie/BEL6405.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9422 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEL/6405 BEL/6405 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intègraleAdobe Acrobat PDF
Titre : Diversité génétique de l'espèce Aegilops geniculata Roth en Algérie par approches morphologique, biochimique et moléculaire Type de document : texte imprimé Auteurs : Asma Medouri, Auteur ; Douadi Khelifi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 132 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Aegilops geniculata Accession Algérie Diversité Morphologie Gluténines Gliadines RAPD Algeria Diversity Morphology Glutenins Gliadins ??????? ???? ?????? ??????? ?????????? ???????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The study of genetic diversity in a collection of the allotetraploid species Aegilops geniculata Roth(UM) collected in Algeria was assessed using morphological, biochemical and molecular approaches.
This was carried on a wide sampling following a climatic gradient throughout North Algeria. This estimation was preceded by assessing the genetic variability of the diploid progenitors of the tetraploid species (Ae. comosa and Ae. umbellulata) based on gluten proteins. For diploids, a significant variation
was revealed for the three classes of gluten proteins. Several new alleles corresponding to HMW-GS were identified and a nomenclature was proposed as: 8 alleles were found at the Glu-M1 locus (with 5 new alleles) and 3 alleles (with one new) at the Glu-U1 locus. For LMW-GS (B- zone), 14 and 7 alleles were revealed at Glu-M3 and Glu-U3 loci respectively. For gliadins, a typology was established.
Morphological diversity of Ae. geniculata was estimated using 15 descriptors. Individuals were morphologically homogenous and little differences were found between accessions. Results of PCA reveal that traits expressing the wide of the spike distinguished better between accessions. Biochemical analysis of glutenins showed a high level of polymorphism as; 15 alleles were recognized at the GluM1 locus and 12 at the Glu-U1 locus with a new subunit and several new alleles. For B-LMW-GS,
the locus Glu-M3 expressed 28 alleles and the locus Glu-U3 expressed 25 alleles. Thirty-two specific patterns were revealed for C-LMW-GS. With respect to gliadins, 61 polymorphic bands resulting in 35 gliadin blocks were found. The genetic diversity index indicated that ω-gliadins were the most polymorphic, followed by γ- and β-gliadin then α-gliadins. Molecular data based on RAPD showed a high polymorphism as 145 fragments were detected with a level of polymorphism of 79.31%.
The three studied approaches (morphology, proteins and DNA polymorphism) showed relationships between genetic variation of the accessions and ecogeographic conditions. Correlation between morphology and some climatic factors showed that some independent characters were correlated with the winter minimum temperature. The polymorphism detected in gliadins highlighted a strong correlation between ecogeographic parameters and some ω-gliadin bands especially the band B14.
RAPD polymorphism showed a strong correlation with ecological parameters."
Diplôme : Doctorat En ligne : ../theses/biologie/MED6917.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10269 Diversité génétique de l'espèce Aegilops geniculata Roth en Algérie par approches morphologique, biochimique et moléculaire [texte imprimé] / Asma Medouri, Auteur ; Douadi Khelifi, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2016 . - 132 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Aegilops geniculata Accession Algérie Diversité Morphologie Gluténines Gliadines RAPD Algeria Diversity Morphology Glutenins Gliadins ??????? ???? ?????? ??????? ?????????? ???????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The study of genetic diversity in a collection of the allotetraploid species Aegilops geniculata Roth(UM) collected in Algeria was assessed using morphological, biochemical and molecular approaches.
This was carried on a wide sampling following a climatic gradient throughout North Algeria. This estimation was preceded by assessing the genetic variability of the diploid progenitors of the tetraploid species (Ae. comosa and Ae. umbellulata) based on gluten proteins. For diploids, a significant variation
was revealed for the three classes of gluten proteins. Several new alleles corresponding to HMW-GS were identified and a nomenclature was proposed as: 8 alleles were found at the Glu-M1 locus (with 5 new alleles) and 3 alleles (with one new) at the Glu-U1 locus. For LMW-GS (B- zone), 14 and 7 alleles were revealed at Glu-M3 and Glu-U3 loci respectively. For gliadins, a typology was established.
Morphological diversity of Ae. geniculata was estimated using 15 descriptors. Individuals were morphologically homogenous and little differences were found between accessions. Results of PCA reveal that traits expressing the wide of the spike distinguished better between accessions. Biochemical analysis of glutenins showed a high level of polymorphism as; 15 alleles were recognized at the GluM1 locus and 12 at the Glu-U1 locus with a new subunit and several new alleles. For B-LMW-GS,
the locus Glu-M3 expressed 28 alleles and the locus Glu-U3 expressed 25 alleles. Thirty-two specific patterns were revealed for C-LMW-GS. With respect to gliadins, 61 polymorphic bands resulting in 35 gliadin blocks were found. The genetic diversity index indicated that ω-gliadins were the most polymorphic, followed by γ- and β-gliadin then α-gliadins. Molecular data based on RAPD showed a high polymorphism as 145 fragments were detected with a level of polymorphism of 79.31%.
The three studied approaches (morphology, proteins and DNA polymorphism) showed relationships between genetic variation of the accessions and ecogeographic conditions. Correlation between morphology and some climatic factors showed that some independent characters were correlated with the winter minimum temperature. The polymorphism detected in gliadins highlighted a strong correlation between ecogeographic parameters and some ω-gliadin bands especially the band B14.
RAPD polymorphism showed a strong correlation with ecological parameters."
Diplôme : Doctorat En ligne : ../theses/biologie/MED6917.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10269 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MED/6917 MED/6917 Thèse Bibliothèque principale Thèses Disponible
Titre : Diversité des sous-unités gluténines HPM et FPM, sous-unités sécalines HPM et 75Kgamme-sécalines d'une collection de triticales hexzploïdes Type de document : texte imprimé Auteurs : Sihem Amri, Auteur ; Douadi Khelifi, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2011 Importance : 96 f. Format : 31 cm Note générale : Magister
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Diversité triticale hexaploïde gluténines, sécalines HPM FPM SDS-PAGE. Biologie Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/AMR5908.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=5708 Diversité des sous-unités gluténines HPM et FPM, sous-unités sécalines HPM et 75Kgamme-sécalines d'une collection de triticales hexzploïdes [texte imprimé] / Sihem Amri, Auteur ; Douadi Khelifi, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2011 . - 96 f. ; 31 cm.
Magister
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Diversité triticale hexaploïde gluténines, sécalines HPM FPM SDS-PAGE. Biologie Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/AMR5908.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=5708 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AMR/5908 AMR/5908 Thèse Bibliothèque principale Thèses Disponible
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