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Partager le résultat de cette recherche Interroger des sources externesThe effect of Argania spinosa on plasma Homocysteine, Lipids, Antioxidant enzymes and Aortas Sections in Methionine induced Hyperhomocysteinemia in mice / Badiaa Aklil
Titre : The effect of Argania spinosa on plasma Homocysteine, Lipids, Antioxidant enzymes and Aortas Sections in Methionine induced Hyperhomocysteinemia in mice Type de document : texte imprimé Auteurs : Badiaa Aklil, Auteur ; Z. Kabouche, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 150 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Homocystéine Argania spinosa Antioxydant Méthionine Stress oxydant Maladies ardiovasculaires Biofilm Homocysteine Antioxidant enzymes Methionine Oxidative
stress Cardiovascular diseases الهوموسيستيين Argania spinosaالإنزيمات المضادة للأكسدة ميثيونين الإجهاد التأكسدي أمراض القلب والأوعية الدموية البيوفيلمIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Hyperhomocysteinemia (HHcy), oxidative stress and decreased antioxidant capacities lead to several clinical manifestations and particularly, cardiovascular and liver diseases. In this study, we evaluated the protective and the preventive effect of the crude extract of Argania spinosa against HHcy ,hyperlipidemia, oxidant status and damages in the aorta, heart and liver induced by high L-methionine intake in mice. After 3 weeks of treatments, Hcy concentrations, lipid parameters, liver enzyme activities, hepatic antioxidant status and histological sections of aorta, liver and heart were determined. Our results showed that consumption of high L-methionine diet (500mg/Kg)
led to an increase in plasma Hcy, CHO, LDL-c, TG, AST, corresponding with decrease of HDL-c, reduced GSH, and catalase activity. These were associated with the loss and degeneration of endothelium, fenestration and formation of foam cells in the media of the aorta, also the alteration of the cardiac muscle and liver tissue. However, the administration of the crude extract of A. spinosa (150mg/Kg) in combination with L-methionine ameliorated all these changes.
Moreover, in the present thesis, we conducted a study in vitro to evaluate the antibacterial effect of Argan oil on biofilm formation. The results showed that there was a trend of increasing inhibition of 4 species of bacteria (Streptococcus mutans, Streptococcus anginosus, Streptococcus intermedius, and staphylococcus haemolyticus), that belong to gram positive strain as the Argan oil was getting more concentrated.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AKL7340.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10990 The effect of Argania spinosa on plasma Homocysteine, Lipids, Antioxidant enzymes and Aortas Sections in Methionine induced Hyperhomocysteinemia in mice [texte imprimé] / Badiaa Aklil, Auteur ; Z. Kabouche, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 150 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Homocystéine Argania spinosa Antioxydant Méthionine Stress oxydant Maladies ardiovasculaires Biofilm Homocysteine Antioxidant enzymes Methionine Oxidative
stress Cardiovascular diseases الهوموسيستيين Argania spinosaالإنزيمات المضادة للأكسدة ميثيونين الإجهاد التأكسدي أمراض القلب والأوعية الدموية البيوفيلمIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Hyperhomocysteinemia (HHcy), oxidative stress and decreased antioxidant capacities lead to several clinical manifestations and particularly, cardiovascular and liver diseases. In this study, we evaluated the protective and the preventive effect of the crude extract of Argania spinosa against HHcy ,hyperlipidemia, oxidant status and damages in the aorta, heart and liver induced by high L-methionine intake in mice. After 3 weeks of treatments, Hcy concentrations, lipid parameters, liver enzyme activities, hepatic antioxidant status and histological sections of aorta, liver and heart were determined. Our results showed that consumption of high L-methionine diet (500mg/Kg)
led to an increase in plasma Hcy, CHO, LDL-c, TG, AST, corresponding with decrease of HDL-c, reduced GSH, and catalase activity. These were associated with the loss and degeneration of endothelium, fenestration and formation of foam cells in the media of the aorta, also the alteration of the cardiac muscle and liver tissue. However, the administration of the crude extract of A. spinosa (150mg/Kg) in combination with L-methionine ameliorated all these changes.
Moreover, in the present thesis, we conducted a study in vitro to evaluate the antibacterial effect of Argan oil on biofilm formation. The results showed that there was a trend of increasing inhibition of 4 species of bacteria (Streptococcus mutans, Streptococcus anginosus, Streptococcus intermedius, and staphylococcus haemolyticus), that belong to gram positive strain as the Argan oil was getting more concentrated.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AKL7340.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10990 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AKL/7340 AKL/7340 Thèse Bibliothèque principale Thèses Disponible
Titre : Étude des mécanismes de résistance de bactéries endophytes isolées à partir des racines d’Hedysarum pallidum et de Lygeum spartum poussant sur des déblais de mine d’antimoine. Type de document : texte imprimé Auteurs : Mounia Kassa Laouar ép Chouikh, Auteur ; Aicha Mechakra (Née Maza), Directeur de thèse ; Oualida Rached-Mosbah, Directeur de thèse Mention d'édition : 09/07/2020 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2020 Importance : 183 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biochimie et Biologie Cellulaire et Moléculaire: Biochimie-Microbiologie appliquée bactéries endophytes métalloïdes stress oxydatif H2O2 MDA proline enzymes
antioxydantes biofilm transformation résistance bacterial endophytes metalloids oxidative stress antioxidant enzymes resistance البكتيريا الداخلية المعادن الثقيلة الإجهاد التأكسدي H2O2البرولين إنزيمات مضادات الأكسدة المقاومة التحولIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
This work focuses on the study of the endophytic bacterial flora of H. pallidum and L. spartum roots, growing on the soil of mining region polluted by Sb and As, and the determination of the physiological, biochemical and molecular defense mechanisms adopted by this microflora to fight against metallic stress. The morphological and biochemical analyzes reveal a high biodiversity of this endophytic microflora. The study of the Sb and As toxicity allows the selection of two strains having the highest MICs, namely 450 mM of Sb and 175 mM of As. The molecular identification of resistant strains via 16S RNA gene sequencing shows that they are identical and belong to the Serratia marcescens species with 99% homology. The batch culture in presence of gradual Sb and As concentrations reveals a progressive decrease in bacterial growth of the two strains. Moreover, the measurement of oxidative stress parameters indicates a significant increase in the H2O2 amount, the MDA amount, the intracellular proline and even the activities of antioxidant enzymes, except at high thresholds in Sb and As. These enzymes permit the detoxification of the bacteria by elimination of the H2O2 and the other free radicals generated by metallic stress. In addition, the study of the resistance of S. marcescens strains to a range of heavy metals and antibiotics shows multiple resistance to nickel and cobalt, as well as to kanamycin, ampicillin and chloramphenicol. To cope with the presence of Sb and As, S. marcescens has adopted life in group mode and form biofilms to reduce their toxicity. The molecular study of the resistance mechanisms of the two strains, using cell transformation methods, reveals that the genetic determinants responsibles for the resistance of S. marcescens to metalloids are carried mainly by the chromosome.Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KAS7638.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11472 Étude des mécanismes de résistance de bactéries endophytes isolées à partir des racines d’Hedysarum pallidum et de Lygeum spartum poussant sur des déblais de mine d’antimoine. [texte imprimé] / Mounia Kassa Laouar ép Chouikh, Auteur ; Aicha Mechakra (Née Maza), Directeur de thèse ; Oualida Rached-Mosbah, Directeur de thèse . - 09/07/2020 . - جامعة الإخوة منتوري قسنطينة, 2020 . - 183 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biochimie et Biologie Cellulaire et Moléculaire: Biochimie-Microbiologie appliquée bactéries endophytes métalloïdes stress oxydatif H2O2 MDA proline enzymes
antioxydantes biofilm transformation résistance bacterial endophytes metalloids oxidative stress antioxidant enzymes resistance البكتيريا الداخلية المعادن الثقيلة الإجهاد التأكسدي H2O2البرولين إنزيمات مضادات الأكسدة المقاومة التحولIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
This work focuses on the study of the endophytic bacterial flora of H. pallidum and L. spartum roots, growing on the soil of mining region polluted by Sb and As, and the determination of the physiological, biochemical and molecular defense mechanisms adopted by this microflora to fight against metallic stress. The morphological and biochemical analyzes reveal a high biodiversity of this endophytic microflora. The study of the Sb and As toxicity allows the selection of two strains having the highest MICs, namely 450 mM of Sb and 175 mM of As. The molecular identification of resistant strains via 16S RNA gene sequencing shows that they are identical and belong to the Serratia marcescens species with 99% homology. The batch culture in presence of gradual Sb and As concentrations reveals a progressive decrease in bacterial growth of the two strains. Moreover, the measurement of oxidative stress parameters indicates a significant increase in the H2O2 amount, the MDA amount, the intracellular proline and even the activities of antioxidant enzymes, except at high thresholds in Sb and As. These enzymes permit the detoxification of the bacteria by elimination of the H2O2 and the other free radicals generated by metallic stress. In addition, the study of the resistance of S. marcescens strains to a range of heavy metals and antibiotics shows multiple resistance to nickel and cobalt, as well as to kanamycin, ampicillin and chloramphenicol. To cope with the presence of Sb and As, S. marcescens has adopted life in group mode and form biofilms to reduce their toxicity. The molecular study of the resistance mechanisms of the two strains, using cell transformation methods, reveals that the genetic determinants responsibles for the resistance of S. marcescens to metalloids are carried mainly by the chromosome.Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KAS7638.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11472 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KAS/7638 KAS/7638 Thèse Bibliothèque principale Thèses Disponible
