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Partager le résultat de cette recherche Interroger des sources externesThe effect of Argania spinosa on plasma Homocysteine, Lipids, Antioxidant enzymes and Aortas Sections in Methionine induced Hyperhomocysteinemia in mice / Badiaa Aklil
Titre : The effect of Argania spinosa on plasma Homocysteine, Lipids, Antioxidant enzymes and Aortas Sections in Methionine induced Hyperhomocysteinemia in mice Type de document : texte imprimé Auteurs : Badiaa Aklil, Auteur ; Z. Kabouche, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 150 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Homocystéine Argania spinosa Antioxydant Méthionine Stress oxydant Maladies ardiovasculaires Biofilm Homocysteine Antioxidant enzymes Methionine Oxidative
stress Cardiovascular diseases الهوموسيستيين Argania spinosaالإنزيمات المضادة للأكسدة ميثيونين الإجهاد التأكسدي أمراض القلب والأوعية الدموية البيوفيلمIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Hyperhomocysteinemia (HHcy), oxidative stress and decreased antioxidant capacities lead to several clinical manifestations and particularly, cardiovascular and liver diseases. In this study, we evaluated the protective and the preventive effect of the crude extract of Argania spinosa against HHcy ,hyperlipidemia, oxidant status and damages in the aorta, heart and liver induced by high L-methionine intake in mice. After 3 weeks of treatments, Hcy concentrations, lipid parameters, liver enzyme activities, hepatic antioxidant status and histological sections of aorta, liver and heart were determined. Our results showed that consumption of high L-methionine diet (500mg/Kg)
led to an increase in plasma Hcy, CHO, LDL-c, TG, AST, corresponding with decrease of HDL-c, reduced GSH, and catalase activity. These were associated with the loss and degeneration of endothelium, fenestration and formation of foam cells in the media of the aorta, also the alteration of the cardiac muscle and liver tissue. However, the administration of the crude extract of A. spinosa (150mg/Kg) in combination with L-methionine ameliorated all these changes.
Moreover, in the present thesis, we conducted a study in vitro to evaluate the antibacterial effect of Argan oil on biofilm formation. The results showed that there was a trend of increasing inhibition of 4 species of bacteria (Streptococcus mutans, Streptococcus anginosus, Streptococcus intermedius, and staphylococcus haemolyticus), that belong to gram positive strain as the Argan oil was getting more concentrated.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AKL7340.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10990 The effect of Argania spinosa on plasma Homocysteine, Lipids, Antioxidant enzymes and Aortas Sections in Methionine induced Hyperhomocysteinemia in mice [texte imprimé] / Badiaa Aklil, Auteur ; Z. Kabouche, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 150 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Homocystéine Argania spinosa Antioxydant Méthionine Stress oxydant Maladies ardiovasculaires Biofilm Homocysteine Antioxidant enzymes Methionine Oxidative
stress Cardiovascular diseases الهوموسيستيين Argania spinosaالإنزيمات المضادة للأكسدة ميثيونين الإجهاد التأكسدي أمراض القلب والأوعية الدموية البيوفيلمIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Hyperhomocysteinemia (HHcy), oxidative stress and decreased antioxidant capacities lead to several clinical manifestations and particularly, cardiovascular and liver diseases. In this study, we evaluated the protective and the preventive effect of the crude extract of Argania spinosa against HHcy ,hyperlipidemia, oxidant status and damages in the aorta, heart and liver induced by high L-methionine intake in mice. After 3 weeks of treatments, Hcy concentrations, lipid parameters, liver enzyme activities, hepatic antioxidant status and histological sections of aorta, liver and heart were determined. Our results showed that consumption of high L-methionine diet (500mg/Kg)
led to an increase in plasma Hcy, CHO, LDL-c, TG, AST, corresponding with decrease of HDL-c, reduced GSH, and catalase activity. These were associated with the loss and degeneration of endothelium, fenestration and formation of foam cells in the media of the aorta, also the alteration of the cardiac muscle and liver tissue. However, the administration of the crude extract of A. spinosa (150mg/Kg) in combination with L-methionine ameliorated all these changes.
Moreover, in the present thesis, we conducted a study in vitro to evaluate the antibacterial effect of Argan oil on biofilm formation. The results showed that there was a trend of increasing inhibition of 4 species of bacteria (Streptococcus mutans, Streptococcus anginosus, Streptococcus intermedius, and staphylococcus haemolyticus), that belong to gram positive strain as the Argan oil was getting more concentrated.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AKL7340.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10990 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AKL/7340 AKL/7340 Thèse Bibliothèque principale Thèses Disponible 
Titre : Effet comparatif de l’extrait butanolique de la plante Stachys mialhesi avec les vitamines B9 et B12 sur quelques paramètres biochimiques et la structure de l’aorte chez des souris induites par une hyperhomocystéinémie Type de document : texte imprimé Auteurs : Samira Boudebouz, Auteur ; S Zerizer, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 104 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Homocystéine Stachys mialhesi Vitamines B9 et B12 Cholestérol total LDL HDL Triglycérides Aorte Homocysteine Vitamins B9 and B12, Total cholesterol Triglycerides Aorta الهوسستیین فیتامین ب 9 و ب 12 الكولسترول الكلي الجلیسریدات الثلاثیة الأورطي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Homocysteine (Hcy) is a sulfur amino acid, synthesized from the metabolism of Lmethionine.
There is a balance in the metabolism of Hcy at physiological conditions.
At high level, the hyperhomocysteinemia (HHcy), considered as an independent risk factor in atherosclerosis, which can cause cellular and molecular alterations.
In the present study, we have measured thiol amino acid (homocysteine), lipidic statut (total cholesterol, LDL, HDL, triglycerides), and activity of transaminases (GOT, GPT) in mice administered with (400mg/kg) of L-methionine during 21 days. Following oral administration of L-methionine in high dose, its degradation product Hcy which is markedly
elevated in plasma and exerts an angiotoxic action directed to the iliac aorta and the other organs (liver, heart and kidneys). This is showed degradation and desquamation of endothelial cells also formation of foam cells in the media part with oval nuclei in mediocytes.
However, the aortic intima of the groups of mice that had been fed with L-methionine plus S.
mialhesi extract (500mg/kg) and also with vitamins B9 and B12 (0,7mg/kg and 0,75μg/kg), respectively showed intact endothelium and spindle-shaped mediocytes nuclei. Nevertheless, oval-shaped mediocytes nuclei and a few foam cells were observed. In the histological sections of the cardiac muscle, liver and kidneys no lysis was observed.
The vitamins B9 and B12 serve as cofactors in the metabolism of L-methionine. So, the vitamins therapy and the extract of S. mialhesi in mice have reduced Hcy, total cholesterol, triglycerides and LDL however, the concentration of HDL was increased.
The extract of medicinal plant S. mialhesi used in this study has antioxidant effect as the vitamins B9 and B12 in mice.
Our results obtained in this study showed that S. mialhesi can be considered as natural source in the prevention against cardiovascular disease.Diplôme : Magistère En ligne : ../theses/biologie/BOU6451.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9486 Effet comparatif de l’extrait butanolique de la plante Stachys mialhesi avec les vitamines B9 et B12 sur quelques paramètres biochimiques et la structure de l’aorte chez des souris induites par une hyperhomocystéinémie [texte imprimé] / Samira Boudebouz, Auteur ; S Zerizer, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 104 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Homocystéine Stachys mialhesi Vitamines B9 et B12 Cholestérol total LDL HDL Triglycérides Aorte Homocysteine Vitamins B9 and B12, Total cholesterol Triglycerides Aorta الهوسستیین فیتامین ب 9 و ب 12 الكولسترول الكلي الجلیسریدات الثلاثیة الأورطي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Homocysteine (Hcy) is a sulfur amino acid, synthesized from the metabolism of Lmethionine.
There is a balance in the metabolism of Hcy at physiological conditions.
At high level, the hyperhomocysteinemia (HHcy), considered as an independent risk factor in atherosclerosis, which can cause cellular and molecular alterations.
In the present study, we have measured thiol amino acid (homocysteine), lipidic statut (total cholesterol, LDL, HDL, triglycerides), and activity of transaminases (GOT, GPT) in mice administered with (400mg/kg) of L-methionine during 21 days. Following oral administration of L-methionine in high dose, its degradation product Hcy which is markedly
elevated in plasma and exerts an angiotoxic action directed to the iliac aorta and the other organs (liver, heart and kidneys). This is showed degradation and desquamation of endothelial cells also formation of foam cells in the media part with oval nuclei in mediocytes.
However, the aortic intima of the groups of mice that had been fed with L-methionine plus S.
mialhesi extract (500mg/kg) and also with vitamins B9 and B12 (0,7mg/kg and 0,75μg/kg), respectively showed intact endothelium and spindle-shaped mediocytes nuclei. Nevertheless, oval-shaped mediocytes nuclei and a few foam cells were observed. In the histological sections of the cardiac muscle, liver and kidneys no lysis was observed.
The vitamins B9 and B12 serve as cofactors in the metabolism of L-methionine. So, the vitamins therapy and the extract of S. mialhesi in mice have reduced Hcy, total cholesterol, triglycerides and LDL however, the concentration of HDL was increased.
The extract of medicinal plant S. mialhesi used in this study has antioxidant effect as the vitamins B9 and B12 in mice.
Our results obtained in this study showed that S. mialhesi can be considered as natural source in the prevention against cardiovascular disease.Diplôme : Magistère En ligne : ../theses/biologie/BOU6451.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9486 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/6451 BOU/6451 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intégraleAdobe Acrobat PDF
Titre : Effets de l’homocystéine sur les processus angiogéniques et l’invasion tumorale du tissu endothélialin vivo et in vitro Type de document : texte imprimé Auteurs : Chérifa Aggoun, Auteur ; Dalila Naimi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2015 Importance : 120 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : تولد الأوعية غزو الأورام عامل النمو الباطني الوعائية مسارات الإشارات الجرذ
Homocystéine Angiogenèse Invasion tumorale VEFG-A Voies de signalisation Rat HUVECs Homocysteine Angiogenesis Tumor invasion Signaling pathways Rat
Physiologie Cellulaire et Moléculaire Signalisation et CancerIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Angiogenesis is the formation of new blood vessels from pre-existing vessels, existing in many pathophysiological conditions, including tumorogenesis. In the activation of the endothelial cell (EC),VEGF-A (vascular endothelial growth factor) is the decisive stage of the process. Since homocysteine (Hcy) has an angiotoxic power, the aim of this work is to assess (in vivo and in vitro studies) the effect of this molecule on angiogenesis and tumor invasion involving VEGF and its two powerful signaling effectors: the HIF(hypoxia –inducible factor )
-1α and the OPN (Osteopontin).
In the in vivo study, the conception of our Hyperhomocysteinemia model (HH) is
confirmed by biochemical study (total Hcy, total protein, CRP and MDA) and histological one (aorta, liver and thymus). We showed that HH potentiated the presence of VEGF-A in thymus, suggesting the installation of a reparative angiogenesis effect, in the same time to an inhibition of VEGF-A and HIF-1α expressions in aortic tissue.
In the in vitro study, we evaluated the antiangiogenic effect of Hcy previously
revealed in vivo, by exposing HUVECs to cytotoxic and pathophysiological concentrations of Hcy. In fact, the first range revealed a strong inhibition of the proliferation, migration and capillary sprouting associated with senescence and apoptosis of EC. In pathophysiological range, inhibition is always dose -dependent and became completely significant in adenosine condition. Hcy seems to act by an hypomethylation mechanism. In addition, the results of qRT-PCR revealed that Hcy inhibited gene expression of VEGF-A and OPN in dosedependent manner.
This study shows a new aspect of antiangiogenic properties of Hcy, by impaired the
expression of genes involved in tumor angiogenesis.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AGG6808.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10061 Effets de l’homocystéine sur les processus angiogéniques et l’invasion tumorale du tissu endothélialin vivo et in vitro [texte imprimé] / Chérifa Aggoun, Auteur ; Dalila Naimi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2015 . - 120 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : تولد الأوعية غزو الأورام عامل النمو الباطني الوعائية مسارات الإشارات الجرذ
Homocystéine Angiogenèse Invasion tumorale VEFG-A Voies de signalisation Rat HUVECs Homocysteine Angiogenesis Tumor invasion Signaling pathways Rat
Physiologie Cellulaire et Moléculaire Signalisation et CancerIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Angiogenesis is the formation of new blood vessels from pre-existing vessels, existing in many pathophysiological conditions, including tumorogenesis. In the activation of the endothelial cell (EC),VEGF-A (vascular endothelial growth factor) is the decisive stage of the process. Since homocysteine (Hcy) has an angiotoxic power, the aim of this work is to assess (in vivo and in vitro studies) the effect of this molecule on angiogenesis and tumor invasion involving VEGF and its two powerful signaling effectors: the HIF(hypoxia –inducible factor )
-1α and the OPN (Osteopontin).
In the in vivo study, the conception of our Hyperhomocysteinemia model (HH) is
confirmed by biochemical study (total Hcy, total protein, CRP and MDA) and histological one (aorta, liver and thymus). We showed that HH potentiated the presence of VEGF-A in thymus, suggesting the installation of a reparative angiogenesis effect, in the same time to an inhibition of VEGF-A and HIF-1α expressions in aortic tissue.
In the in vitro study, we evaluated the antiangiogenic effect of Hcy previously
revealed in vivo, by exposing HUVECs to cytotoxic and pathophysiological concentrations of Hcy. In fact, the first range revealed a strong inhibition of the proliferation, migration and capillary sprouting associated with senescence and apoptosis of EC. In pathophysiological range, inhibition is always dose -dependent and became completely significant in adenosine condition. Hcy seems to act by an hypomethylation mechanism. In addition, the results of qRT-PCR revealed that Hcy inhibited gene expression of VEGF-A and OPN in dosedependent manner.
This study shows a new aspect of antiangiogenic properties of Hcy, by impaired the
expression of genes involved in tumor angiogenesis.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AGG6808.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10061 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AGG/6808 AGG/6808 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude de certains polymorphismes génétiques dans les métabolismes des folates et de l’homocystéine et anomalies de fermeture du tube neural Type de document : texte imprimé Auteurs : Amel Abbas ép. Tebdjoune, Auteur ; Nourredine Abadi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 152 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Polymorphismes MTHFR C677T MTHFR A1298C MTR A2756G MTRR A66G RFC A80G CBS 844ins68 AFTN FOLATE HOMOCYSTEINE NTD 0التشوھات الخلقیةلانغلاق الأنبوب العصبي حمض الفولیك ھوموسستیین Index. décimale : 570 Sciences de la vie. Biologie Résumé : Neural tube defects (NTDs) represent a major cause of infant mortality among congenital malformations, whose pathogenesis remains poorly understood. In this study realized on an algerian population of affected children and their mothers, we investigated the frequency of many polymorphisms involved in the of folates / homocysteine metabolisms, Their possible contribution in the etiology of NTDs, as well as their influence on folates and homocysteine concentrations.
The mutations were determined by the PCR / RFLP method, the assay of the biochemical parameters was performed by chemiluminescence.
The analysis of genetic polymorphisms has shown that AG and AA variants of the G80A polymorphism of the RFC gene are associated with the increased risk in affected children.
Moreover, we have observed that the combined effect of C677T and A1298C polymorphisms in MTHFR gene, confers a risk for the occurrence of these abnormalities in our population .Indeed, statistical test of interaction between the two polymorphisms have shown that the combination of heterozygous genotypes(MTHFR 677CT/1298AC) elevate the risk in NTD cases (p <0.05).
These two polymorphisms ,in MTHFR gene, affects homocysteine metabolism in mothers of NTD cases leading to homocysteine concentration values higher in mothers with TT genotype of the C677T (p < 0.05) and that of AA genotype for the polymorphism A1298C (p <0.05).
For the polymorphism A2756G of the MTR gene, Although, Its association with NTDs risk appears to be negative, It was found to decrease the RBC folate level strengthen the contribution of A2756G mutation in NTDs occurrence (p <0.05).
Data on the association between MTRR A66G, CBS 844ins68 polymorphisms and plasma homocysteine and serum/RBC folate concentrations as well as the relationship between this polymorphisms and NTDs risk are inconclusive.
This original study by its primacy in Algeria and also in North Africa, both in terms of the diversity of polymorphisms examined and the nature of the population (children affected) deserves, encourages the extension of the study to a larger sample In order to show the interactive effect of these mutations on the occurrence of NTDs and on the rate of metabolites.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ABB7166.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10447 Etude de certains polymorphismes génétiques dans les métabolismes des folates et de l’homocystéine et anomalies de fermeture du tube neural [texte imprimé] / Amel Abbas ép. Tebdjoune, Auteur ; Nourredine Abadi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 152 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Polymorphismes MTHFR C677T MTHFR A1298C MTR A2756G MTRR A66G RFC A80G CBS 844ins68 AFTN FOLATE HOMOCYSTEINE NTD 0التشوھات الخلقیةلانغلاق الأنبوب العصبي حمض الفولیك ھوموسستیین Index. décimale : 570 Sciences de la vie. Biologie Résumé : Neural tube defects (NTDs) represent a major cause of infant mortality among congenital malformations, whose pathogenesis remains poorly understood. In this study realized on an algerian population of affected children and their mothers, we investigated the frequency of many polymorphisms involved in the of folates / homocysteine metabolisms, Their possible contribution in the etiology of NTDs, as well as their influence on folates and homocysteine concentrations.
The mutations were determined by the PCR / RFLP method, the assay of the biochemical parameters was performed by chemiluminescence.
The analysis of genetic polymorphisms has shown that AG and AA variants of the G80A polymorphism of the RFC gene are associated with the increased risk in affected children.
Moreover, we have observed that the combined effect of C677T and A1298C polymorphisms in MTHFR gene, confers a risk for the occurrence of these abnormalities in our population .Indeed, statistical test of interaction between the two polymorphisms have shown that the combination of heterozygous genotypes(MTHFR 677CT/1298AC) elevate the risk in NTD cases (p <0.05).
These two polymorphisms ,in MTHFR gene, affects homocysteine metabolism in mothers of NTD cases leading to homocysteine concentration values higher in mothers with TT genotype of the C677T (p < 0.05) and that of AA genotype for the polymorphism A1298C (p <0.05).
For the polymorphism A2756G of the MTR gene, Although, Its association with NTDs risk appears to be negative, It was found to decrease the RBC folate level strengthen the contribution of A2756G mutation in NTDs occurrence (p <0.05).
Data on the association between MTRR A66G, CBS 844ins68 polymorphisms and plasma homocysteine and serum/RBC folate concentrations as well as the relationship between this polymorphisms and NTDs risk are inconclusive.
This original study by its primacy in Algeria and also in North Africa, both in terms of the diversity of polymorphisms examined and the nature of the population (children affected) deserves, encourages the extension of the study to a larger sample In order to show the interactive effect of these mutations on the occurrence of NTDs and on the rate of metabolites.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ABB7166.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10447 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ABB/7166 ABB/7166 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude des marqueurs biologiques (lipides, homocystéine, CRP) et génétiques (MTHFR) dans l'infractus du myocarde (IDM) Type de document : texte imprimé Auteurs : Ouarda Semmame, Auteur ; N. Abadi, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2008 Importance : 112 f. Format : 31 cm Note générale : Magister
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Lipide IDM Arthérosclérose Homocystéine CRP Méthylènetétrahydrofolate réductase MTHFR Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/SEM5095.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1379 Etude des marqueurs biologiques (lipides, homocystéine, CRP) et génétiques (MTHFR) dans l'infractus du myocarde (IDM) [texte imprimé] / Ouarda Semmame, Auteur ; N. Abadi, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2008 . - 112 f. ; 31 cm.
Magister
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Lipide IDM Arthérosclérose Homocystéine CRP Méthylènetétrahydrofolate réductase MTHFR Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/SEM5095.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1379 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité SEM/5095 SEM/5095 Thèse Bibliothèque principale Thèses Disponible
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