| Titre : |
Sélection in vitro pour la tolérance au stress hydrique chez le blé dur (Triticum durum Desf) : approche protéomique, transcriptomique et génétique. |
| Type de document : |
texte imprimé |
| Auteurs : |
Nadia Sandra Kacem, Auteur ; Abdelhamid Djekoun, Directeur de thèse ; Yordan Muhovski, Directeur de thèse |
| Editeur : |
جامعة الإخوة منتوري قسنطينة |
| Année de publication : |
2017 |
| Note générale : |
2 copies imprimées disponibles
|
| Langues : |
Français (fre) |
| Tags : |
Durum wheat water stress PEG 6000 in vitro selection somaclonal
variation molecular marker diagonal two-dimensional electrophoresis , qRT-PCR Western blot Blé dur électrophorèse diagonale bidimensionnelle marqueur moléculaire sélection in vitro stress hydrique variation somaclonale |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
The in vitro evaluation of three durum wheat genotypes (Djenah Khetifa, Oued Zenati
and Waha( to water stress tolerance simulated in vitro by different concentrations of
PEG 6000 indicated significant differences among genotypes, PEG concentrations and
their interactions for all the studied characters, indicating genetic variability in PEGsimulated drought stress. The osmotic pressure has negatively affected all parameters
studied. However, cells that continue to grow under severe osmotic stress are
considered as tolerant to water stress. In vitro screening for stress tolerance reveals the
variety Djenah Khetifa as the most tolerant and Waha as sensitive. The studied traits can
be recommended as suitable selection criteria for screening drought-tolerant genotypes.
Three amplified and reproducible locus (gwm331, gwm335 and gwm427) were retained.
These loci provided in total 78 monomorphic loci of which 69 were detected in somaclones.
Five somaclonal variants (DKR1-S1, DKR1-S2, OZR1-S2, WR1-C3 and WR1-S1) were
identified by only two SSR markers. Genetic variation rate was 21.74%. The presence of
selective agent in the medium could explain the observed genetic variations. Somaclonal
variation cannot always be detected at the gross morphological level. The selected SSR
markers could be used to study the uniformity of plants obtained from tissue culture and
varietal identification. Analysis of the proteins by a new two-dimensional diagonal
electrophoresis approach named (D-2DE) followed by MALDI-TOF mass spectrometry
has identified six differentially expressed proteins. For each variety, the level of mRNA
expression of three candidate genes (globulin, GAPDH and peroxidase) and a
ubiquitous (Actine) gene was determined by qRT-PCR. The amounts of mRNA of the
three genes are partially correlated with the amounts of proteins. These genes were
slightly up-regulated over the control. The D-2DE results were validated using Western
blot analysis with specific anti-globulin and anti-glyceraldehyde in the embryogenic
calli of wheat, in response to water stress. The proteins identified through this technique
provide new insights as to how calli respond to osmotic stress. Our method of study
provides an original and relevant approach that could contribute significantly to a better
understanding of possible linkages between the abundance of specific proteins and the
acclimation of durum wheat to water stress at the cellular level. |
| Diplôme : |
Doctorat en sciences |
| En ligne : |
../theses/biologie/KAC7070.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10531 |
Sélection in vitro pour la tolérance au stress hydrique chez le blé dur (Triticum durum Desf) : approche protéomique, transcriptomique et génétique. [texte imprimé] / Nadia Sandra Kacem, Auteur ; Abdelhamid Djekoun, Directeur de thèse ; Yordan Muhovski, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2017. 2 copies imprimées disponibles
Langues : Français ( fre)
| Tags : |
Durum wheat water stress PEG 6000 in vitro selection somaclonal
variation molecular marker diagonal two-dimensional electrophoresis , qRT-PCR Western blot Blé dur électrophorèse diagonale bidimensionnelle marqueur moléculaire sélection in vitro stress hydrique variation somaclonale |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
The in vitro evaluation of three durum wheat genotypes (Djenah Khetifa, Oued Zenati
and Waha( to water stress tolerance simulated in vitro by different concentrations of
PEG 6000 indicated significant differences among genotypes, PEG concentrations and
their interactions for all the studied characters, indicating genetic variability in PEGsimulated drought stress. The osmotic pressure has negatively affected all parameters
studied. However, cells that continue to grow under severe osmotic stress are
considered as tolerant to water stress. In vitro screening for stress tolerance reveals the
variety Djenah Khetifa as the most tolerant and Waha as sensitive. The studied traits can
be recommended as suitable selection criteria for screening drought-tolerant genotypes.
Three amplified and reproducible locus (gwm331, gwm335 and gwm427) were retained.
These loci provided in total 78 monomorphic loci of which 69 were detected in somaclones.
Five somaclonal variants (DKR1-S1, DKR1-S2, OZR1-S2, WR1-C3 and WR1-S1) were
identified by only two SSR markers. Genetic variation rate was 21.74%. The presence of
selective agent in the medium could explain the observed genetic variations. Somaclonal
variation cannot always be detected at the gross morphological level. The selected SSR
markers could be used to study the uniformity of plants obtained from tissue culture and
varietal identification. Analysis of the proteins by a new two-dimensional diagonal
electrophoresis approach named (D-2DE) followed by MALDI-TOF mass spectrometry
has identified six differentially expressed proteins. For each variety, the level of mRNA
expression of three candidate genes (globulin, GAPDH and peroxidase) and a
ubiquitous (Actine) gene was determined by qRT-PCR. The amounts of mRNA of the
three genes are partially correlated with the amounts of proteins. These genes were
slightly up-regulated over the control. The D-2DE results were validated using Western
blot analysis with specific anti-globulin and anti-glyceraldehyde in the embryogenic
calli of wheat, in response to water stress. The proteins identified through this technique
provide new insights as to how calli respond to osmotic stress. Our method of study
provides an original and relevant approach that could contribute significantly to a better
understanding of possible linkages between the abundance of specific proteins and the
acclimation of durum wheat to water stress at the cellular level. |
| Diplôme : |
Doctorat en sciences |
| En ligne : |
../theses/biologie/KAC7070.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10531 |
|
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