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Titre : Interactions génotype-milieu de variétés de blé tendre (Triticum aestivum L.) sous stress hydrique Type de document : texte imprimé Auteurs : Karima Kara, Auteur ; Louhichi Brinis, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2015 Importance : 200 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Triticum aestivum stress hydrique marqueurs physiologiques paramètres agronomiques marqueurs biochimiques marqueurs technologiques interaction génotypesmilieu water stress physiological markers agronomic parameters biochemical markers technological markers genotype-environment interaction الإجهاد المائي علامات فسيولوجية متغيرات زراعية علامات بيوكيميائية علامات تكنولوجية تفاعل وراثي- بيئي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Knowledge of the physiological, biochemical, agronomic and technological traits involved in plant adaptation process to abiotic stresses is a decisive step in the fight against drought. This study is a contribution to understanding the mechanisms of tolerance to water stress in bread wheat varieties Triticum aestivum L. and to establish genotype-environment interactions to evaluate the genotypic variability.
In the first series of tests, the study shows that the varieties introduced Cham 6 Hamam 1 and Attila are the most productive. The local variety Ain Abid followed by Attila of Angi-4 are the most tolerant to water stress.
The second experimental trial is to test during two campaigns, 10 bread wheat lines at three different geo-climatic sites: Setif, Constantine and Guelma. The results of phenological, morphological and agronomic parameters fluctuate over time and space. The earliest species provide lower performance parameters to Guelma, while the later varieties give the best yields in Constantine. Local varieties Ain Abid, Arz and introduced line Prl / 2 * Pastor provide the best yield and its components on Setif sites. This last line with Pastor / Wbll1 and Hidhab feature a performance by high yield and components of yield on Constantine and Guelma.
The interaction genotype-environment is very highly significant between all studied agronomic parameters.
On the site of Setif (semi-arid) are rated the highest levels of total protein for Thelin line // 2 * Attila * 2 / Pastor, while Prl / 2 * Pastor accumulates the most important starch contents on Constantine site. Total protein levels are higher on experimental testing of Setif, while higher levels of starch and moisture are obtained for all varieties grown on Constantine and Guelma. The genotype-environment interaction is highly significant for all biochemical and technological parameters. The data show that the line Kauz / Pastor / Fiscal has the highest values W (dough strength) to G (swelling of dough), it also gives the best Pelshenke compared to other lines, it is followed by Pastor / Wbll1 and Thelin // 2 * Attila * 2 / Pastor lines. The latter are characterized by a very high baking strength (strength of wheat). It's on the sites of Setif and Guelma that are expressed wheat lines with the most efficient technological parameters.
The wide variability in gluten strength, character controlled mainly by the allelic variation of loci Glu-A1; Glu-B1; Glu-D1, gives an idea about the genetic diversity obtained by expression and composition of these loci. Gluten, rheological parameters alveograph (W, P), Pelshenke test and SDS sedimentation volume vary as a function of the allelic diversity HMW glutenins. The results show the superiority of the alleles 22, (6 + 8), (7 + 9), (2 + 12), (10 + 5) and (7 + 8), compared to other alleles controlling these parameters.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KAR6756.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9859 Interactions génotype-milieu de variétés de blé tendre (Triticum aestivum L.) sous stress hydrique [texte imprimé] / Karima Kara, Auteur ; Louhichi Brinis, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2015 . - 200 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Triticum aestivum stress hydrique marqueurs physiologiques paramètres agronomiques marqueurs biochimiques marqueurs technologiques interaction génotypesmilieu water stress physiological markers agronomic parameters biochemical markers technological markers genotype-environment interaction الإجهاد المائي علامات فسيولوجية متغيرات زراعية علامات بيوكيميائية علامات تكنولوجية تفاعل وراثي- بيئي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Knowledge of the physiological, biochemical, agronomic and technological traits involved in plant adaptation process to abiotic stresses is a decisive step in the fight against drought. This study is a contribution to understanding the mechanisms of tolerance to water stress in bread wheat varieties Triticum aestivum L. and to establish genotype-environment interactions to evaluate the genotypic variability.
In the first series of tests, the study shows that the varieties introduced Cham 6 Hamam 1 and Attila are the most productive. The local variety Ain Abid followed by Attila of Angi-4 are the most tolerant to water stress.
The second experimental trial is to test during two campaigns, 10 bread wheat lines at three different geo-climatic sites: Setif, Constantine and Guelma. The results of phenological, morphological and agronomic parameters fluctuate over time and space. The earliest species provide lower performance parameters to Guelma, while the later varieties give the best yields in Constantine. Local varieties Ain Abid, Arz and introduced line Prl / 2 * Pastor provide the best yield and its components on Setif sites. This last line with Pastor / Wbll1 and Hidhab feature a performance by high yield and components of yield on Constantine and Guelma.
The interaction genotype-environment is very highly significant between all studied agronomic parameters.
On the site of Setif (semi-arid) are rated the highest levels of total protein for Thelin line // 2 * Attila * 2 / Pastor, while Prl / 2 * Pastor accumulates the most important starch contents on Constantine site. Total protein levels are higher on experimental testing of Setif, while higher levels of starch and moisture are obtained for all varieties grown on Constantine and Guelma. The genotype-environment interaction is highly significant for all biochemical and technological parameters. The data show that the line Kauz / Pastor / Fiscal has the highest values W (dough strength) to G (swelling of dough), it also gives the best Pelshenke compared to other lines, it is followed by Pastor / Wbll1 and Thelin // 2 * Attila * 2 / Pastor lines. The latter are characterized by a very high baking strength (strength of wheat). It's on the sites of Setif and Guelma that are expressed wheat lines with the most efficient technological parameters.
The wide variability in gluten strength, character controlled mainly by the allelic variation of loci Glu-A1; Glu-B1; Glu-D1, gives an idea about the genetic diversity obtained by expression and composition of these loci. Gluten, rheological parameters alveograph (W, P), Pelshenke test and SDS sedimentation volume vary as a function of the allelic diversity HMW glutenins. The results show the superiority of the alleles 22, (6 + 8), (7 + 9), (2 + 12), (10 + 5) and (7 + 8), compared to other alleles controlling these parameters.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KAR6756.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9859 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KAR/6756 KAR/6756 Thèse Bibliothèque principale Thèses Disponible 
Titre : Contribution à la connaissance des stress biotiques affectant les céréales d'hiver : « Identification et approche à l'étude épidémiologique du virus de la jaunisse nanisante de L’Orge (VJNO) ou (BYDV) sévissant dans les cultures des céréales dans les zones Est de l’Algérie ». Type de document : texte imprimé Auteurs : Ammar Ouffroukh, Auteur ; Douadi Khelifi, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 111 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Céréales Maladies Virus BYDV Sérologie ELISA souches Pathotype (Pav, Mav, Rpv,) Pucerons stress hydrique Epidémiologie Cereal diseases viruses Serology strains Aphids water stress Epidemiology الحبوب الأمراض الفيروسات الأمصال السلالات أنثير طائرات المن ندرة المياه علم الأوبئة Index. décimale : 570 Sciences de la vie. Biologie Résumé : Research on cereals in recent years, Indicate the risk of epidemic development of certain fungal diseases (rust yellow blotch, Septoria, etc.), and viral adequate measures of prevention and intervention are not taken in time (A.Ouffroukh. 2008; A.Ouffroukh, 2006). Crop losses arising are important. In favorable years the Yellow rust, for example, can take extents epidemic and destroy entire harvests. (A.Ouffroukh., 2008; A.Ouffroukh., 2006; B. Bahri, M. Leconte, A. Ouffroukh, C. Vallavieille-Pope, J. Enjalbert, 2009). Viral diseases in turn, are taking extension. Thus, the Barley Yellow Dwarf Virus (BYDV.) or (BYDV) was reported in several areas prospected and several cereal species (wheat and barley hard and soft).
In general very little information is available on their epidemiology and economic impact, when the means of struggle, they have little or almost nonexistent (H. Stone and JP Signoret, 1978). BYDV (BYDV) little known in Algeria compared to other diseases, can be very important for our cereal and threatening, particularly since this disease is transmitted by insect vectors belonging to the genus including Aphidideae, including a dozen some species have been identified (A.Ouffroukh., 2006; L. Ayadi K. and Ayoub, 1994; Ouffroukh. A Khelifi. D, Dehimet. L, 2009). Also after his observation and detection, identity veered ¬ was confirmed by serological investigations have shown that the existence of two viral strains or Pathotypes (PAV and MAV), confirming the results obtained by H. Belkahla.et Lapierre (1999).
The frequency of the disease varies annually from 8% to 18% depending on the region but is also encountered and generally on the different species at rates ranging from 8% to 28%. (L. Ayadi K. and Ayoub, 1994; A. Ouffroukh., 2008; Ouffroukh. A Khelifi. D, Dehimet. L, 2009).
In terms of capacity vector, PR appears to be best vector and infected plants by this species appear most affected clinically, about the impact of contamination on cereal species studied, it appears that the barley species are most affected, while soft wheats are more susceptible to BYDV (BYDV) and durum wheats.
Moreover, the influence of virus Barley yellow dwarf (BYDV) on cereal development is certain and it is especially important that the plant is subjected to water stress, it's more 'is that cultivars and genotypes most tolerant in general show a certain level of tolerance to drought.
In epidemiological terms, and although the results we have achieved can not definitively conclude on the precise conditions of the regions studied, the fact remains that valuable information was obtained. It appears here the whole point estimates of aphid that are a fundamental element .indication of risk to crops and therefore the periods in which the actions of prevention and protection should be carried out.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/OUF6463.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9503 Contribution à la connaissance des stress biotiques affectant les céréales d'hiver : « Identification et approche à l'étude épidémiologique du virus de la jaunisse nanisante de L’Orge (VJNO) ou (BYDV) sévissant dans les cultures des céréales dans les zones Est de l’Algérie ». [texte imprimé] / Ammar Ouffroukh, Auteur ; Douadi Khelifi, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 111 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Céréales Maladies Virus BYDV Sérologie ELISA souches Pathotype (Pav, Mav, Rpv,) Pucerons stress hydrique Epidémiologie Cereal diseases viruses Serology strains Aphids water stress Epidemiology الحبوب الأمراض الفيروسات الأمصال السلالات أنثير طائرات المن ندرة المياه علم الأوبئة Index. décimale : 570 Sciences de la vie. Biologie Résumé : Research on cereals in recent years, Indicate the risk of epidemic development of certain fungal diseases (rust yellow blotch, Septoria, etc.), and viral adequate measures of prevention and intervention are not taken in time (A.Ouffroukh. 2008; A.Ouffroukh, 2006). Crop losses arising are important. In favorable years the Yellow rust, for example, can take extents epidemic and destroy entire harvests. (A.Ouffroukh., 2008; A.Ouffroukh., 2006; B. Bahri, M. Leconte, A. Ouffroukh, C. Vallavieille-Pope, J. Enjalbert, 2009). Viral diseases in turn, are taking extension. Thus, the Barley Yellow Dwarf Virus (BYDV.) or (BYDV) was reported in several areas prospected and several cereal species (wheat and barley hard and soft).
In general very little information is available on their epidemiology and economic impact, when the means of struggle, they have little or almost nonexistent (H. Stone and JP Signoret, 1978). BYDV (BYDV) little known in Algeria compared to other diseases, can be very important for our cereal and threatening, particularly since this disease is transmitted by insect vectors belonging to the genus including Aphidideae, including a dozen some species have been identified (A.Ouffroukh., 2006; L. Ayadi K. and Ayoub, 1994; Ouffroukh. A Khelifi. D, Dehimet. L, 2009). Also after his observation and detection, identity veered ¬ was confirmed by serological investigations have shown that the existence of two viral strains or Pathotypes (PAV and MAV), confirming the results obtained by H. Belkahla.et Lapierre (1999).
The frequency of the disease varies annually from 8% to 18% depending on the region but is also encountered and generally on the different species at rates ranging from 8% to 28%. (L. Ayadi K. and Ayoub, 1994; A. Ouffroukh., 2008; Ouffroukh. A Khelifi. D, Dehimet. L, 2009).
In terms of capacity vector, PR appears to be best vector and infected plants by this species appear most affected clinically, about the impact of contamination on cereal species studied, it appears that the barley species are most affected, while soft wheats are more susceptible to BYDV (BYDV) and durum wheats.
Moreover, the influence of virus Barley yellow dwarf (BYDV) on cereal development is certain and it is especially important that the plant is subjected to water stress, it's more 'is that cultivars and genotypes most tolerant in general show a certain level of tolerance to drought.
In epidemiological terms, and although the results we have achieved can not definitively conclude on the precise conditions of the regions studied, the fact remains that valuable information was obtained. It appears here the whole point estimates of aphid that are a fundamental element .indication of risk to crops and therefore the periods in which the actions of prevention and protection should be carried out.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/OUF6463.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9503 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité OUF/6463 OUF/6463 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intégraleAdobe Acrobat PDF
Titre : Etude des enzymes du stress oxydatif chez le blé dur (Triticum durum Desf.) : caractérisation biochimique et moléculaire. Type de document : texte imprimé Auteurs : Karima Bouchemal, Auteur ; Abdelhamid Djekoun, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 172 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Blé dur stress hydrique antioxydants isoenzymes stress oxydatif peroxydase catalase superoxyde dismutase expression génique Durum wheat water stress antioxidants oxydative stress peroxidase superoxide dismutase gene expression القمح الصلب الإجهاد المائي مضادات الأكسدة الإنزيمات المتشابهة الإجهاد التأكسدي التعبير الوراثيperoxydase Index. décimale : 570 Sciences de la vie. Biologie Résumé : Plant drought tolerance requires the activation of complex metabolic including antioxidative pathways, especially reactive oxygen species (ROS) scavenging enzymatic systems. In this study, 10 durum wheat genotypes (Triticum durum Desf.) were evaluated under drought stress conditions, in order to highlight the correlations between genetic diversity identified through enzymatic markers : superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and water stress tolerance properties. Firstly, in the first assay, we characterized the impact of oxidative stress and assessed the performance of different cultivars of durum wheat under drought, heat and paraquat stress. The activities of enzymatic antioxidants SOD, CAT, GPOX and oxidative biomarkers were measured : lipid peroxidation, H2O2 content, membrane stability, and total chlorophyll content. In addition, a correlation analysis between antioxidant enzymes, oxidative markers and physiological parameters related to water status was performed. Results indicated that the 3 treatments decreased membrane stability, chlorophyll content and increased lipid peroxidation, H2O2 content and activities of antioxidant enzymes.However, there were significant differences between wheat cultivars in amounts of decrease or increase in the measured traits. Correlation analysis between biochemical and physiological parameters revealed the singularity of SOD enzyme, which is particularly related to the relative water content, stomatal conductance and total chlorophyll content, The second part of this thesis was devoted to the study of the genetic variability of the three foliar enzymatic systems which allowed us to refine the results of the biochemical and physiological analysis as well as to highlight the influence of water stress on the metabolism of these enzymes in wheat plants grown hydroponically and treated with PEG 6000. An approach based on native PAGE has been developed to evaluate whether the observed overall variations, resulted from qualitative (presence/absence) or quantitative variations specific to certain isoenzymes. Marked differences were detected in the profiles of the studied 3 enzymatic systems studied. Electrophoretic analysis showed the induction of new isoforms and an increase in their intensity in durum wheat cultivars under water stress. Finally, the study of the expression of genes coding for these enzymes, in leaf cells of durum wheat was conducted through RealTime quantitative RT-PCR. Expression levels of 3 genes coding for CAT (TdCAT1, CATA, CAT3), 3 genes coding for POX (Pox3, TaPrx107, TaPrx115) and 2 genes coding for SOD (TdMnSOD, SOD1.1) were differently affected by drought. A variation in transcript accumulation was observed in response to water stress. This molecular approach identified the genes CATA, CAT3, TdMnSOD and SOD1.1 as candidate genes for potential genetic engineering targets and research for molecular markers as a selection tool for improving the tolerance of durum wheat to water stress. Diplôme : Doctorat En ligne : ../theses/biologie/BOU7398.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11048 Etude des enzymes du stress oxydatif chez le blé dur (Triticum durum Desf.) : caractérisation biochimique et moléculaire. [texte imprimé] / Karima Bouchemal, Auteur ; Abdelhamid Djekoun, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 172 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Blé dur stress hydrique antioxydants isoenzymes stress oxydatif peroxydase catalase superoxyde dismutase expression génique Durum wheat water stress antioxidants oxydative stress peroxidase superoxide dismutase gene expression القمح الصلب الإجهاد المائي مضادات الأكسدة الإنزيمات المتشابهة الإجهاد التأكسدي التعبير الوراثيperoxydase Index. décimale : 570 Sciences de la vie. Biologie Résumé : Plant drought tolerance requires the activation of complex metabolic including antioxidative pathways, especially reactive oxygen species (ROS) scavenging enzymatic systems. In this study, 10 durum wheat genotypes (Triticum durum Desf.) were evaluated under drought stress conditions, in order to highlight the correlations between genetic diversity identified through enzymatic markers : superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and water stress tolerance properties. Firstly, in the first assay, we characterized the impact of oxidative stress and assessed the performance of different cultivars of durum wheat under drought, heat and paraquat stress. The activities of enzymatic antioxidants SOD, CAT, GPOX and oxidative biomarkers were measured : lipid peroxidation, H2O2 content, membrane stability, and total chlorophyll content. In addition, a correlation analysis between antioxidant enzymes, oxidative markers and physiological parameters related to water status was performed. Results indicated that the 3 treatments decreased membrane stability, chlorophyll content and increased lipid peroxidation, H2O2 content and activities of antioxidant enzymes.However, there were significant differences between wheat cultivars in amounts of decrease or increase in the measured traits. Correlation analysis between biochemical and physiological parameters revealed the singularity of SOD enzyme, which is particularly related to the relative water content, stomatal conductance and total chlorophyll content, The second part of this thesis was devoted to the study of the genetic variability of the three foliar enzymatic systems which allowed us to refine the results of the biochemical and physiological analysis as well as to highlight the influence of water stress on the metabolism of these enzymes in wheat plants grown hydroponically and treated with PEG 6000. An approach based on native PAGE has been developed to evaluate whether the observed overall variations, resulted from qualitative (presence/absence) or quantitative variations specific to certain isoenzymes. Marked differences were detected in the profiles of the studied 3 enzymatic systems studied. Electrophoretic analysis showed the induction of new isoforms and an increase in their intensity in durum wheat cultivars under water stress. Finally, the study of the expression of genes coding for these enzymes, in leaf cells of durum wheat was conducted through RealTime quantitative RT-PCR. Expression levels of 3 genes coding for CAT (TdCAT1, CATA, CAT3), 3 genes coding for POX (Pox3, TaPrx107, TaPrx115) and 2 genes coding for SOD (TdMnSOD, SOD1.1) were differently affected by drought. A variation in transcript accumulation was observed in response to water stress. This molecular approach identified the genes CATA, CAT3, TdMnSOD and SOD1.1 as candidate genes for potential genetic engineering targets and research for molecular markers as a selection tool for improving the tolerance of durum wheat to water stress. Diplôme : Doctorat En ligne : ../theses/biologie/BOU7398.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11048 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/7398 BOU/7398 Thèse Bibliothèque principale Thèses Disponible
Titre : Sélection in vitro pour la tolérance au stress hydrique chez le blé dur (Triticum durum Desf) : approche protéomique, transcriptomique et génétique. Type de document : texte imprimé Auteurs : Nadia Sandra Kacem, Auteur ; Abdelhamid Djekoun, Directeur de thèse ; Yordan Muhovski, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Durum wheat water stress PEG 6000 in vitro selection somaclonal
variation molecular marker diagonal two-dimensional electrophoresis , qRT-PCR Western blot Blé dur électrophorèse diagonale bidimensionnelle marqueur moléculaire sélection in vitro stress hydrique variation somaclonaleIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The in vitro evaluation of three durum wheat genotypes (Djenah Khetifa, Oued Zenati
and Waha( to water stress tolerance simulated in vitro by different concentrations of
PEG 6000 indicated significant differences among genotypes, PEG concentrations and
their interactions for all the studied characters, indicating genetic variability in PEGsimulated drought stress. The osmotic pressure has negatively affected all parameters
studied. However, cells that continue to grow under severe osmotic stress are
considered as tolerant to water stress. In vitro screening for stress tolerance reveals the
variety Djenah Khetifa as the most tolerant and Waha as sensitive. The studied traits can
be recommended as suitable selection criteria for screening drought-tolerant genotypes.
Three amplified and reproducible locus (gwm331, gwm335 and gwm427) were retained.
These loci provided in total 78 monomorphic loci of which 69 were detected in somaclones.
Five somaclonal variants (DKR1-S1, DKR1-S2, OZR1-S2, WR1-C3 and WR1-S1) were
identified by only two SSR markers. Genetic variation rate was 21.74%. The presence of
selective agent in the medium could explain the observed genetic variations. Somaclonal
variation cannot always be detected at the gross morphological level. The selected SSR
markers could be used to study the uniformity of plants obtained from tissue culture and
varietal identification. Analysis of the proteins by a new two-dimensional diagonal
electrophoresis approach named (D-2DE) followed by MALDI-TOF mass spectrometry
has identified six differentially expressed proteins. For each variety, the level of mRNA
expression of three candidate genes (globulin, GAPDH and peroxidase) and a
ubiquitous (Actine) gene was determined by qRT-PCR. The amounts of mRNA of the
three genes are partially correlated with the amounts of proteins. These genes were
slightly up-regulated over the control. The D-2DE results were validated using Western
blot analysis with specific anti-globulin and anti-glyceraldehyde in the embryogenic
calli of wheat, in response to water stress. The proteins identified through this technique
provide new insights as to how calli respond to osmotic stress. Our method of study
provides an original and relevant approach that could contribute significantly to a better
understanding of possible linkages between the abundance of specific proteins and the
acclimation of durum wheat to water stress at the cellular level.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KAC7070.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10531 Sélection in vitro pour la tolérance au stress hydrique chez le blé dur (Triticum durum Desf) : approche protéomique, transcriptomique et génétique. [texte imprimé] / Nadia Sandra Kacem, Auteur ; Abdelhamid Djekoun, Directeur de thèse ; Yordan Muhovski, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Durum wheat water stress PEG 6000 in vitro selection somaclonal
variation molecular marker diagonal two-dimensional electrophoresis , qRT-PCR Western blot Blé dur électrophorèse diagonale bidimensionnelle marqueur moléculaire sélection in vitro stress hydrique variation somaclonaleIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The in vitro evaluation of three durum wheat genotypes (Djenah Khetifa, Oued Zenati
and Waha( to water stress tolerance simulated in vitro by different concentrations of
PEG 6000 indicated significant differences among genotypes, PEG concentrations and
their interactions for all the studied characters, indicating genetic variability in PEGsimulated drought stress. The osmotic pressure has negatively affected all parameters
studied. However, cells that continue to grow under severe osmotic stress are
considered as tolerant to water stress. In vitro screening for stress tolerance reveals the
variety Djenah Khetifa as the most tolerant and Waha as sensitive. The studied traits can
be recommended as suitable selection criteria for screening drought-tolerant genotypes.
Three amplified and reproducible locus (gwm331, gwm335 and gwm427) were retained.
These loci provided in total 78 monomorphic loci of which 69 were detected in somaclones.
Five somaclonal variants (DKR1-S1, DKR1-S2, OZR1-S2, WR1-C3 and WR1-S1) were
identified by only two SSR markers. Genetic variation rate was 21.74%. The presence of
selective agent in the medium could explain the observed genetic variations. Somaclonal
variation cannot always be detected at the gross morphological level. The selected SSR
markers could be used to study the uniformity of plants obtained from tissue culture and
varietal identification. Analysis of the proteins by a new two-dimensional diagonal
electrophoresis approach named (D-2DE) followed by MALDI-TOF mass spectrometry
has identified six differentially expressed proteins. For each variety, the level of mRNA
expression of three candidate genes (globulin, GAPDH and peroxidase) and a
ubiquitous (Actine) gene was determined by qRT-PCR. The amounts of mRNA of the
three genes are partially correlated with the amounts of proteins. These genes were
slightly up-regulated over the control. The D-2DE results were validated using Western
blot analysis with specific anti-globulin and anti-glyceraldehyde in the embryogenic
calli of wheat, in response to water stress. The proteins identified through this technique
provide new insights as to how calli respond to osmotic stress. Our method of study
provides an original and relevant approach that could contribute significantly to a better
understanding of possible linkages between the abundance of specific proteins and the
acclimation of durum wheat to water stress at the cellular level.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KAC7070.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10531 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KAC/7070 KAC/7070 Thèse Bibliothèque principale Thèses Disponible
Titre : Caractètes physiologiques et biochimiques de tolérance du blé dur (Triticum durum Desf.) au strss hydrique Type de document : texte imprimé Auteurs : Adra Mouellef, Auteur ; Univ. de Constantine, Éditeur scientifique ; N. Ykhlef, Directeur de thèse Année de publication : 2010 Importance : 69 f. Note générale : 01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Morphologie Blé dur Electrophorèse Culture in vitro SDS-PAGE Tolérance PEG Strss hydrique Water stress tolerance durum wheat morphology physiology and biochemistry in vitro culture SDS-PAGE electrophoresis الإجهاد المائي المقاومة القمح الصلب مرفولولوجيا فيزيولوجيا بيوكميا الزراعة في الأنابيب PEG 600 الهجرة الكهربائية SDS-PEGA Index. décimale : 570 Sciences de la vie. Biologie Résumé : The objective of this work is to study the effect of water stress, and variability of response in seven genotypes of durum wheat (Triticum durum Desf): Djenah khetaifa, Bidi 17, Guem goum Rkham, Rahouia, Beliouni, Vitron and Waha.
In the first part, we studied various morphological parameters, biochemical and physiological under five irrigation levels (100, 80, 50, 25 and 12.5% of field capacity); the choice of levels of irrigation is to apply at third leaf. The results show that water stress caused a reduction in leaf area, decreased water content, rate of water loss and the rate of total chlorophyll. Similarly, increased specific leaf weight, stomatal resistance and accumulation of prolin and soluble sugars are stored.
In the second part, were analyzed by SDS-PAGE total protein expressed in leaf and root the same genotypes after water stress applied in vitro by addition of PEG 600 to 7%. The electrophoresis diagrams, we give these subunits both in controls than in stress,while others are present or absent in stressed and witnesses.
In conclusion, the study showed that water stress causes the same response mechanisms among seven genotypes but to different degrees.Diplôme : Magistère En ligne : ../theses/biologie/MOU5728.pdf Permalink : index.php?lvl=notice_display&id=1829 Caractètes physiologiques et biochimiques de tolérance du blé dur (Triticum durum Desf.) au strss hydrique [texte imprimé] / Adra Mouellef, Auteur ; Univ. de Constantine, Éditeur scientifique ; N. Ykhlef, Directeur de thèse . - 2010 . - 69 f.
01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Morphologie Blé dur Electrophorèse Culture in vitro SDS-PAGE Tolérance PEG Strss hydrique Water stress tolerance durum wheat morphology physiology and biochemistry in vitro culture SDS-PAGE electrophoresis الإجهاد المائي المقاومة القمح الصلب مرفولولوجيا فيزيولوجيا بيوكميا الزراعة في الأنابيب PEG 600 الهجرة الكهربائية SDS-PEGA Index. décimale : 570 Sciences de la vie. Biologie Résumé : The objective of this work is to study the effect of water stress, and variability of response in seven genotypes of durum wheat (Triticum durum Desf): Djenah khetaifa, Bidi 17, Guem goum Rkham, Rahouia, Beliouni, Vitron and Waha.
In the first part, we studied various morphological parameters, biochemical and physiological under five irrigation levels (100, 80, 50, 25 and 12.5% of field capacity); the choice of levels of irrigation is to apply at third leaf. The results show that water stress caused a reduction in leaf area, decreased water content, rate of water loss and the rate of total chlorophyll. Similarly, increased specific leaf weight, stomatal resistance and accumulation of prolin and soluble sugars are stored.
In the second part, were analyzed by SDS-PAGE total protein expressed in leaf and root the same genotypes after water stress applied in vitro by addition of PEG 600 to 7%. The electrophoresis diagrams, we give these subunits both in controls than in stress,while others are present or absent in stressed and witnesses.
In conclusion, the study showed that water stress causes the same response mechanisms among seven genotypes but to different degrees.Diplôme : Magistère En ligne : ../theses/biologie/MOU5728.pdf Permalink : index.php?lvl=notice_display&id=1829 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MOU/5728 MOU/5728 Thèse Bibliothèque principale Thèses Disponible
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