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Titre : Etude des enzymes du stress oxydatif chez le blé dur (Triticum durum Desf.) : caractérisation biochimique et moléculaire. Type de document : texte imprimé Auteurs : Karima Bouchemal, Auteur ; Abdelhamid Djekoun, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 172 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Blé dur stress hydrique antioxydants isoenzymes stress oxydatif peroxydase catalase superoxyde dismutase expression génique Durum wheat water stress antioxidants oxydative stress peroxidase superoxide dismutase gene expression القمح الصلب الإجهاد المائي مضادات الأكسدة الإنزيمات المتشابهة الإجهاد التأكسدي التعبير الوراثيperoxydase Index. décimale : 570 Sciences de la vie. Biologie Résumé : Plant drought tolerance requires the activation of complex metabolic including antioxidative pathways, especially reactive oxygen species (ROS) scavenging enzymatic systems. In this study, 10 durum wheat genotypes (Triticum durum Desf.) were evaluated under drought stress conditions, in order to highlight the correlations between genetic diversity identified through enzymatic markers : superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and water stress tolerance properties. Firstly, in the first assay, we characterized the impact of oxidative stress and assessed the performance of different cultivars of durum wheat under drought, heat and paraquat stress. The activities of enzymatic antioxidants SOD, CAT, GPOX and oxidative biomarkers were measured : lipid peroxidation, H2O2 content, membrane stability, and total chlorophyll content. In addition, a correlation analysis between antioxidant enzymes, oxidative markers and physiological parameters related to water status was performed. Results indicated that the 3 treatments decreased membrane stability, chlorophyll content and increased lipid peroxidation, H2O2 content and activities of antioxidant enzymes.However, there were significant differences between wheat cultivars in amounts of decrease or increase in the measured traits. Correlation analysis between biochemical and physiological parameters revealed the singularity of SOD enzyme, which is particularly related to the relative water content, stomatal conductance and total chlorophyll content, The second part of this thesis was devoted to the study of the genetic variability of the three foliar enzymatic systems which allowed us to refine the results of the biochemical and physiological analysis as well as to highlight the influence of water stress on the metabolism of these enzymes in wheat plants grown hydroponically and treated with PEG 6000. An approach based on native PAGE has been developed to evaluate whether the observed overall variations, resulted from qualitative (presence/absence) or quantitative variations specific to certain isoenzymes. Marked differences were detected in the profiles of the studied 3 enzymatic systems studied. Electrophoretic analysis showed the induction of new isoforms and an increase in their intensity in durum wheat cultivars under water stress. Finally, the study of the expression of genes coding for these enzymes, in leaf cells of durum wheat was conducted through RealTime quantitative RT-PCR. Expression levels of 3 genes coding for CAT (TdCAT1, CATA, CAT3), 3 genes coding for POX (Pox3, TaPrx107, TaPrx115) and 2 genes coding for SOD (TdMnSOD, SOD1.1) were differently affected by drought. A variation in transcript accumulation was observed in response to water stress. This molecular approach identified the genes CATA, CAT3, TdMnSOD and SOD1.1 as candidate genes for potential genetic engineering targets and research for molecular markers as a selection tool for improving the tolerance of durum wheat to water stress. Diplôme : Doctorat En ligne : ../theses/biologie/BOU7398.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11048 Etude des enzymes du stress oxydatif chez le blé dur (Triticum durum Desf.) : caractérisation biochimique et moléculaire. [texte imprimé] / Karima Bouchemal, Auteur ; Abdelhamid Djekoun, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 172 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Blé dur stress hydrique antioxydants isoenzymes stress oxydatif peroxydase catalase superoxyde dismutase expression génique Durum wheat water stress antioxidants oxydative stress peroxidase superoxide dismutase gene expression القمح الصلب الإجهاد المائي مضادات الأكسدة الإنزيمات المتشابهة الإجهاد التأكسدي التعبير الوراثيperoxydase Index. décimale : 570 Sciences de la vie. Biologie Résumé : Plant drought tolerance requires the activation of complex metabolic including antioxidative pathways, especially reactive oxygen species (ROS) scavenging enzymatic systems. In this study, 10 durum wheat genotypes (Triticum durum Desf.) were evaluated under drought stress conditions, in order to highlight the correlations between genetic diversity identified through enzymatic markers : superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and water stress tolerance properties. Firstly, in the first assay, we characterized the impact of oxidative stress and assessed the performance of different cultivars of durum wheat under drought, heat and paraquat stress. The activities of enzymatic antioxidants SOD, CAT, GPOX and oxidative biomarkers were measured : lipid peroxidation, H2O2 content, membrane stability, and total chlorophyll content. In addition, a correlation analysis between antioxidant enzymes, oxidative markers and physiological parameters related to water status was performed. Results indicated that the 3 treatments decreased membrane stability, chlorophyll content and increased lipid peroxidation, H2O2 content and activities of antioxidant enzymes.However, there were significant differences between wheat cultivars in amounts of decrease or increase in the measured traits. Correlation analysis between biochemical and physiological parameters revealed the singularity of SOD enzyme, which is particularly related to the relative water content, stomatal conductance and total chlorophyll content, The second part of this thesis was devoted to the study of the genetic variability of the three foliar enzymatic systems which allowed us to refine the results of the biochemical and physiological analysis as well as to highlight the influence of water stress on the metabolism of these enzymes in wheat plants grown hydroponically and treated with PEG 6000. An approach based on native PAGE has been developed to evaluate whether the observed overall variations, resulted from qualitative (presence/absence) or quantitative variations specific to certain isoenzymes. Marked differences were detected in the profiles of the studied 3 enzymatic systems studied. Electrophoretic analysis showed the induction of new isoforms and an increase in their intensity in durum wheat cultivars under water stress. Finally, the study of the expression of genes coding for these enzymes, in leaf cells of durum wheat was conducted through RealTime quantitative RT-PCR. Expression levels of 3 genes coding for CAT (TdCAT1, CATA, CAT3), 3 genes coding for POX (Pox3, TaPrx107, TaPrx115) and 2 genes coding for SOD (TdMnSOD, SOD1.1) were differently affected by drought. A variation in transcript accumulation was observed in response to water stress. This molecular approach identified the genes CATA, CAT3, TdMnSOD and SOD1.1 as candidate genes for potential genetic engineering targets and research for molecular markers as a selection tool for improving the tolerance of durum wheat to water stress. Diplôme : Doctorat En ligne : ../theses/biologie/BOU7398.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11048 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/7398 BOU/7398 Thèse Bibliothèque principale Thèses Disponible L’effet protecteur des antioxydants naturels dans l’intoxication du mercure chez le rat Albinos Wistar / Ahlem Bahi
Titre : L’effet protecteur des antioxydants naturels dans l’intoxication du mercure chez le rat Albinos Wistar : (Aspects biochimiques, immunologiques et histologiques). Type de document : texte imprimé Auteurs : Ahlem Bahi, Auteur ; Youcef Necib, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2015 Importance : 158 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biochimie appliquee Mercure Huile d’argan Sélénium Stress oxydatif Argania Spinosa antioxydants Mercury Argan Oil Selenium Oxidative Stress antioxidants السلينيوم الإجهاد التاكسدي زيت الأركان الزئبق Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present study aimed to evaluate the protective effect of argan oil and selenium against oxidative stress induced by mercury. For this reason, a phytochemical study of antioxidant tested in vitro was performed on argan oil to estimate its expected effect. The results showed the presence of phenolic compounds in a very high concentration. The evaluation of power scavenger argan oil is more active with IC50 = 13.33 ± 1.3, and in vivo on Albino Wistar rats were treated with mercury alone or combined with argan oil and selenium, for a period of three weeks, the blood is then withdrawn and the rats are sacrificed the various parameters are determined. From the analysis of our results, we observed a significant reduction in weight gain during treatment with mercury alone or combined with argan oil and selenium, but no clear change in the relative weight of liver, kidney or spleen was obtained. The results also showed significant changes in biochemical parameters, immunological and haematological. Characterized primarily by a significant increase in serum glucose, urea, creatinine, uric acid, total and direct bilirubin, total lipids, cholesterol, triglyceride, conjugated diene and the enzymatic activity of AST, ALT, LDH and PAL .dropoff window Whereas serum concentrations of albumin and total protein were reduced in treated rats compared to mercury control animals. Results also show immunological and haematological toxicity especially among the treated groups the pure mercury, an increase in serum IL-1, IL-6 and TNF and elevated white blood cell count, decreased red blood cell levels, hemoglobin concentration the percentage of hematocrit and phagocytic activity during treatment with mercury. Thus the treatment of the rats with the mercury causes a reduction in the plasma antioxidant capacity characterized by decrease in the serum concentration of vitamin E and C and rate of DPPH and thiols. We also observed that the results obtained highlight the detoxifier potential expressed by the decrease in erythrocyte glutathione levels, liver and kidney, metallothionein, the enzymatic activity of the antioxidant system SOD, GPx, GST and catalase and increased in lipid peroxidation expressed by the high levels of plasma MDA, erythrocyte, liver and kidney. Also the results clearly showed substantive alterations of tissue studies in comparison with the control, with hepatocyte cytolysis, hepatic necrosis and tubular degeneration with necrosis of the kidneys. Furthermore supplementation argan oil and selenium changed the toxic effects of mercury and decreased histological alterations and showed that both antioxidants are effective against oxidative stress induced by mercury
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BAH6696.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9928 L’effet protecteur des antioxydants naturels dans l’intoxication du mercure chez le rat Albinos Wistar : (Aspects biochimiques, immunologiques et histologiques). [texte imprimé] / Ahlem Bahi, Auteur ; Youcef Necib, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2015 . - 158 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biochimie appliquee Mercure Huile d’argan Sélénium Stress oxydatif Argania Spinosa antioxydants Mercury Argan Oil Selenium Oxidative Stress antioxidants السلينيوم الإجهاد التاكسدي زيت الأركان الزئبق Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present study aimed to evaluate the protective effect of argan oil and selenium against oxidative stress induced by mercury. For this reason, a phytochemical study of antioxidant tested in vitro was performed on argan oil to estimate its expected effect. The results showed the presence of phenolic compounds in a very high concentration. The evaluation of power scavenger argan oil is more active with IC50 = 13.33 ± 1.3, and in vivo on Albino Wistar rats were treated with mercury alone or combined with argan oil and selenium, for a period of three weeks, the blood is then withdrawn and the rats are sacrificed the various parameters are determined. From the analysis of our results, we observed a significant reduction in weight gain during treatment with mercury alone or combined with argan oil and selenium, but no clear change in the relative weight of liver, kidney or spleen was obtained. The results also showed significant changes in biochemical parameters, immunological and haematological. Characterized primarily by a significant increase in serum glucose, urea, creatinine, uric acid, total and direct bilirubin, total lipids, cholesterol, triglyceride, conjugated diene and the enzymatic activity of AST, ALT, LDH and PAL .dropoff window Whereas serum concentrations of albumin and total protein were reduced in treated rats compared to mercury control animals. Results also show immunological and haematological toxicity especially among the treated groups the pure mercury, an increase in serum IL-1, IL-6 and TNF and elevated white blood cell count, decreased red blood cell levels, hemoglobin concentration the percentage of hematocrit and phagocytic activity during treatment with mercury. Thus the treatment of the rats with the mercury causes a reduction in the plasma antioxidant capacity characterized by decrease in the serum concentration of vitamin E and C and rate of DPPH and thiols. We also observed that the results obtained highlight the detoxifier potential expressed by the decrease in erythrocyte glutathione levels, liver and kidney, metallothionein, the enzymatic activity of the antioxidant system SOD, GPx, GST and catalase and increased in lipid peroxidation expressed by the high levels of plasma MDA, erythrocyte, liver and kidney. Also the results clearly showed substantive alterations of tissue studies in comparison with the control, with hepatocyte cytolysis, hepatic necrosis and tubular degeneration with necrosis of the kidneys. Furthermore supplementation argan oil and selenium changed the toxic effects of mercury and decreased histological alterations and showed that both antioxidants are effective against oxidative stress induced by mercury
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BAH6696.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9928 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BAH/6696 BAH/6696 Thèse Bibliothèque principale Thèses Disponible 
Titre : Enquête sur la consommation des polyphénols auprès d’un échantillon de 200 personnes de la région de Constantine Type de document : texte imprimé Auteurs : Fatima Zohra Becila, Auteur ; Malika Barkat, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 104 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Free radicals oxidative stress antioxidants polyphenols health nutrition radicaux libres apport journalier polyphénols santé alimentation الجذور الحرة الأكسدة مضادات الاكسدة متعدد الفينولات الصحة التغدية Index. décimale : 640 Industrie Alimentaire Résumé : The organism produces free radicals daily. They may result from the action of certain chemical components of our environment (X-rays and UV light) of air pollutants (N, NO2), drugs, smoke, tobacco, alcohol, dietary errors.
Oxidative stress due to free radicals causes tissue damage mainly by oxidation of proteins, DNA or lipids; it is involved in many diseases such as trigger or associated with the development of complications. Antioxidants are compound chemicals that reduce cells’ oxidation. Some are endogenous, while others are obtained from the diet (exogenous). Among them, polyphenols represent a very large proportion of micronutrients provided by our diet. They are becoming increasingly important, particularly caused of their effects of natural antioxidants.
The purpose of the survey is to collect as much information on the consumption of certain foods that contain polyphenols end to know the daily consumption of a sample of 200 subjects taken at random in the Constantine. The questionnaire developed has two main components: a first component summarizes information on the identification of the interviewee, his health, risk factors and sedentary behavior. The second part contains information on foods consumed containing polyphenolic compounds, drawn from the database of the site phenol -explorer (http:// www. Phenol-explorer.eu/contents/total? Food- id = aa1). He also discussed in addition to the quantitative aspect and some processing that may affect the polyphenol composition of foods. From the results obtained, we run down the following key points:
For daily consumption, 76% of subjects surveyed consume vegetables, 48% consume fruits, 39% and 20% drink drinks and herbal teas.
Consumption of vegetables, fruits and beverages depends on the price, while the taste is not the primary factor in daily consumption of herbal teas.
The sick patients consume fewer fresh fruits and vegetables and envelopes, more preserved foods, have more risk factors compared to healthy subjects.
Regarding the consumption of polyphenols majority of respondents (75%) consumption complies with the standard (1g per day) which 2,75% corresponds to sick patients and 92,55% with healthy subjects. Among subjects with the standard lower consumption (25%), 97,43% corresponds to sick patients and 7,45% corresponds to healthy subjects. A weak correlation is found between the consumption of polyphenols and health, A weak correlation is found between the consumption of polyphenols and health.Diplôme : Magistère En ligne : ../theses/agronomie/BEC6482.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9543 Enquête sur la consommation des polyphénols auprès d’un échantillon de 200 personnes de la région de Constantine [texte imprimé] / Fatima Zohra Becila, Auteur ; Malika Barkat, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 104 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Free radicals oxidative stress antioxidants polyphenols health nutrition radicaux libres apport journalier polyphénols santé alimentation الجذور الحرة الأكسدة مضادات الاكسدة متعدد الفينولات الصحة التغدية Index. décimale : 640 Industrie Alimentaire Résumé : The organism produces free radicals daily. They may result from the action of certain chemical components of our environment (X-rays and UV light) of air pollutants (N, NO2), drugs, smoke, tobacco, alcohol, dietary errors.
Oxidative stress due to free radicals causes tissue damage mainly by oxidation of proteins, DNA or lipids; it is involved in many diseases such as trigger or associated with the development of complications. Antioxidants are compound chemicals that reduce cells’ oxidation. Some are endogenous, while others are obtained from the diet (exogenous). Among them, polyphenols represent a very large proportion of micronutrients provided by our diet. They are becoming increasingly important, particularly caused of their effects of natural antioxidants.
The purpose of the survey is to collect as much information on the consumption of certain foods that contain polyphenols end to know the daily consumption of a sample of 200 subjects taken at random in the Constantine. The questionnaire developed has two main components: a first component summarizes information on the identification of the interviewee, his health, risk factors and sedentary behavior. The second part contains information on foods consumed containing polyphenolic compounds, drawn from the database of the site phenol -explorer (http:// www. Phenol-explorer.eu/contents/total? Food- id = aa1). He also discussed in addition to the quantitative aspect and some processing that may affect the polyphenol composition of foods. From the results obtained, we run down the following key points:
For daily consumption, 76% of subjects surveyed consume vegetables, 48% consume fruits, 39% and 20% drink drinks and herbal teas.
Consumption of vegetables, fruits and beverages depends on the price, while the taste is not the primary factor in daily consumption of herbal teas.
The sick patients consume fewer fresh fruits and vegetables and envelopes, more preserved foods, have more risk factors compared to healthy subjects.
Regarding the consumption of polyphenols majority of respondents (75%) consumption complies with the standard (1g per day) which 2,75% corresponds to sick patients and 92,55% with healthy subjects. Among subjects with the standard lower consumption (25%), 97,43% corresponds to sick patients and 7,45% corresponds to healthy subjects. A weak correlation is found between the consumption of polyphenols and health, A weak correlation is found between the consumption of polyphenols and health.Diplôme : Magistère En ligne : ../theses/agronomie/BEC6482.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9543 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEC/6482 BEC/6482 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intégraleAdobe Acrobat PDF
Titre : Impact de la cuisson à l’eau sur la teneur et le profil en composés phénoliques de légumes secs. Type de document : texte imprimé Auteurs : Saliha Djabali, Auteur ; Malika Barkat, Directeur de thèse Mention d'édition : 03/03/2021 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2021 Importance : 179 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Biotechnologie :science Alimentaire Radicaux libres antioxydants activité antiradicalaire composés phénoliques légumes secs cuisson Free radicals antioxidants anti-radical activity phenolic compounds pulses cooking الجذور الحرة مضادات الأكسدة النشاط المضاد للجذور الحرة المركبات الفينولية البقول الطبخ Index. décimale : 640 Industrie Alimentaire Résumé :
The aims of this study are to measure the content of phenolic compounds (polyphenols, flavonoids, tanins and anthocyanins), to estimate the anti-radical and antioxidant activity and to highlight the impact of cooking in water on five species of pulses (lens, chickpeas, peas, common beans and beans) which are widely consumed in Algeria. For comparison, two varieties for each species were studied. To well characterize the polyphenols, flavonoids, anthocyanins and tanins, their profile was determined by the liquid chromatographic method coupled with mass spectroscopy (LCMS / MS). The main results obtained show that the species analyzed all contain phenolic compounds (polyphenols, flavonoids, tannins and anthocyanins) with inter and intra varietal significant differences. Cooking has had a variable effect, sometimes positive and other times negative on the content and the anti- radical and antioxidant activity of the phenolic compounds. The results of the DPPH. test, show that the extract of raw Ibla lens, the extract of the cooking water Ibla lens, the extract of the cooking water of chickpeas (two varieties) and Extracted from the cooking water Oinward peas contained great active compounds capable of reducing this free radical, since they acted at low concentrations. In contrast, the extract of raw Oinward peas was found to be the least effective. Also, the main results obtained from the ABTS. + Test indicates that the extract of cooked beans and their cooking water are the most potent. On the other hand, the extract of cooked Oinward peas proved to be the least effective. In fact, the cooking water has recovered significant amounts which exert significant anti- radical activities, particularly in the case of lens and beans. Similarly, newly formed phenolic compounds have been revealed in the cooking water, this is due to the degradation of the glycosylated forms and to the phenomenon of diffusion. Regarding the CUPRAC test, significant correlations have been established between antioxidant activity and certain phenolic compounds. These activities are either dose-dependent or structure-dependent. The result of the present study confirms that legumes are an important source of phenolic compounds and which are considered to be natural antioxidants. To boost an individual's antioxidant status, it is desirable to consume the cooked seeds and the water from their cooking.
Note de contenu :
Annexes.Diplôme : Doctorat en sciences En ligne : ../theses/agronomie/DJA7728.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11562 Impact de la cuisson à l’eau sur la teneur et le profil en composés phénoliques de légumes secs. [texte imprimé] / Saliha Djabali, Auteur ; Malika Barkat, Directeur de thèse . - 03/03/2021 . - جامعة الإخوة منتوري قسنطينة, 2021 . - 179 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Biotechnologie :science Alimentaire Radicaux libres antioxydants activité antiradicalaire composés phénoliques légumes secs cuisson Free radicals antioxidants anti-radical activity phenolic compounds pulses cooking الجذور الحرة مضادات الأكسدة النشاط المضاد للجذور الحرة المركبات الفينولية البقول الطبخ Index. décimale : 640 Industrie Alimentaire Résumé :
The aims of this study are to measure the content of phenolic compounds (polyphenols, flavonoids, tanins and anthocyanins), to estimate the anti-radical and antioxidant activity and to highlight the impact of cooking in water on five species of pulses (lens, chickpeas, peas, common beans and beans) which are widely consumed in Algeria. For comparison, two varieties for each species were studied. To well characterize the polyphenols, flavonoids, anthocyanins and tanins, their profile was determined by the liquid chromatographic method coupled with mass spectroscopy (LCMS / MS). The main results obtained show that the species analyzed all contain phenolic compounds (polyphenols, flavonoids, tannins and anthocyanins) with inter and intra varietal significant differences. Cooking has had a variable effect, sometimes positive and other times negative on the content and the anti- radical and antioxidant activity of the phenolic compounds. The results of the DPPH. test, show that the extract of raw Ibla lens, the extract of the cooking water Ibla lens, the extract of the cooking water of chickpeas (two varieties) and Extracted from the cooking water Oinward peas contained great active compounds capable of reducing this free radical, since they acted at low concentrations. In contrast, the extract of raw Oinward peas was found to be the least effective. Also, the main results obtained from the ABTS. + Test indicates that the extract of cooked beans and their cooking water are the most potent. On the other hand, the extract of cooked Oinward peas proved to be the least effective. In fact, the cooking water has recovered significant amounts which exert significant anti- radical activities, particularly in the case of lens and beans. Similarly, newly formed phenolic compounds have been revealed in the cooking water, this is due to the degradation of the glycosylated forms and to the phenomenon of diffusion. Regarding the CUPRAC test, significant correlations have been established between antioxidant activity and certain phenolic compounds. These activities are either dose-dependent or structure-dependent. The result of the present study confirms that legumes are an important source of phenolic compounds and which are considered to be natural antioxidants. To boost an individual's antioxidant status, it is desirable to consume the cooked seeds and the water from their cooking.
Note de contenu :
Annexes.Diplôme : Doctorat en sciences En ligne : ../theses/agronomie/DJA7728.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11562 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité DJA/7728 DJA/7728 Thèse Bibliothèque principale Thèses Disponible
Titre : L'effet de la plante Lifago dielsii sur le stress oxydatif/ nitrosatif et apoptotique hépatocytaire induit par les antituberculeux. Type de document : texte imprimé Auteurs : Samiya Baali, Auteur ; Souad Ameddah, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 121 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Isoniazide Rifampicine Lifago dielsii. stress oxydatif et nitrosatif Hépatoprotection Isoniazid Rifampicin oxidative and nitrosative stress Hepatic steatosis Antioxidants Hepato-protection الايزونيازيد-ريفومبيس إضطراب تأكسدي إضطراب نتروزي وقاية الكبد Index. décimale : 570 Sciences de la vie. Biologie Résumé : The purpose of this study is to evaluate in vivo the antioxidant and hepatoprotective effect of the butanolic extract of Lifago dielsii (BELD). The adult male Wistar Albino rats were force-fed with the BELD (250 mg / kg) for a period of 14 days and simultaneously received a combined treatment of the isoniazide (INH, 75mg / kg) and rifampicin (RIF, 150mg / kg). In vitro study of the antioxidant activity of BELD was also performed.
Combination treatment of INH / RIF induces hepatic dysfunctions that have been revealed by; a significant increase in the level of serum enzymes (ALT, AST, ALP, LDH), alteration of lipid profile parameters (HDL, LDL, cholesterol, serum TG and hepatic TG ) as well as those of hepatic nitroso-oxidative stress (MDA, 4HNE, LHP, nitrosothiols, carbonylated proteins, nitric oxide) compared to the controls. Similarly, treatment with INH / RIF induces a significant reduction in the antioxidant activity of SOD, CAT, GST, GPx and POD and the level of GSH in the liver. Histologically, the combination of INH / RIF was able to induce hepatic steatosis. In contrast, the pretreatment of rats with BELD protects the liver against the toxic effect of metabolites generated by INH / RIF, thereby maintaining liver functions and balancing hepatic redox status. It appears from this in vivo study that BELD is endowed with antioxidant and hepatoprotective activity with a positive action on the restoration of INH /
RIF-induced steatosis. The antioxidant effect of BELD has been studied in vitro by carrying out tests such as: reducing power, hydroxyl radical scavenging (HO°), total antioxidant capacity, iron chelating effect, nitrite anti-oxide effect (NO°) and Inhibition of lipid peroxidation by the ferric thiocyanate method. The results obtained show that this extract has a strong scavenger activity of radical HO° and NO°, as well as a strong reductive and inhibitory capacity of lipid peroxidation. In conclusion, the study realized valid the safety and
effectiveness of the plant lifago dielsii. Which is a plant resulting from traditional Algerian medicine. The results obtained also support the therapeutic potential of the Butanolic extract of this plant, proving that its extracts and bioactive compounds can be developed as a new natural product for the prevention of liver injuries.
Diplôme : Doctorat En ligne : ../theses/biologie/BAA7283.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10898 L'effet de la plante Lifago dielsii sur le stress oxydatif/ nitrosatif et apoptotique hépatocytaire induit par les antituberculeux. [texte imprimé] / Samiya Baali, Auteur ; Souad Ameddah, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 121 f. ; 30 cm.
2 copies imprimées disponibles
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BiologieTags : Isoniazide Rifampicine Lifago dielsii. stress oxydatif et nitrosatif Hépatoprotection Isoniazid Rifampicin oxidative and nitrosative stress Hepatic steatosis Antioxidants Hepato-protection الايزونيازيد-ريفومبيس إضطراب تأكسدي إضطراب نتروزي وقاية الكبد Index. décimale : 570 Sciences de la vie. Biologie Résumé : The purpose of this study is to evaluate in vivo the antioxidant and hepatoprotective effect of the butanolic extract of Lifago dielsii (BELD). The adult male Wistar Albino rats were force-fed with the BELD (250 mg / kg) for a period of 14 days and simultaneously received a combined treatment of the isoniazide (INH, 75mg / kg) and rifampicin (RIF, 150mg / kg). In vitro study of the antioxidant activity of BELD was also performed.
Combination treatment of INH / RIF induces hepatic dysfunctions that have been revealed by; a significant increase in the level of serum enzymes (ALT, AST, ALP, LDH), alteration of lipid profile parameters (HDL, LDL, cholesterol, serum TG and hepatic TG ) as well as those of hepatic nitroso-oxidative stress (MDA, 4HNE, LHP, nitrosothiols, carbonylated proteins, nitric oxide) compared to the controls. Similarly, treatment with INH / RIF induces a significant reduction in the antioxidant activity of SOD, CAT, GST, GPx and POD and the level of GSH in the liver. Histologically, the combination of INH / RIF was able to induce hepatic steatosis. In contrast, the pretreatment of rats with BELD protects the liver against the toxic effect of metabolites generated by INH / RIF, thereby maintaining liver functions and balancing hepatic redox status. It appears from this in vivo study that BELD is endowed with antioxidant and hepatoprotective activity with a positive action on the restoration of INH /
RIF-induced steatosis. The antioxidant effect of BELD has been studied in vitro by carrying out tests such as: reducing power, hydroxyl radical scavenging (HO°), total antioxidant capacity, iron chelating effect, nitrite anti-oxide effect (NO°) and Inhibition of lipid peroxidation by the ferric thiocyanate method. The results obtained show that this extract has a strong scavenger activity of radical HO° and NO°, as well as a strong reductive and inhibitory capacity of lipid peroxidation. In conclusion, the study realized valid the safety and
effectiveness of the plant lifago dielsii. Which is a plant resulting from traditional Algerian medicine. The results obtained also support the therapeutic potential of the Butanolic extract of this plant, proving that its extracts and bioactive compounds can be developed as a new natural product for the prevention of liver injuries.
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Code-barres Cote Support Localisation Section Disponibilité BAA/7283 BAA/7283 Thèse Bibliothèque principale Thèses Disponible
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