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Partager le résultat de cette recherche Interroger des sources externesCorrélations entre le comportement dépressif, le profil lipidique et les paramètres du stress oxydatif au cours du diabète expérimental / Redouane Rebia
Titre : Corrélations entre le comportement dépressif, le profil lipidique et les paramètres du stress oxydatif au cours du diabète expérimental : Effet protecteur de la mélatonine et de la fluoxétine Type de document : texte imprimé Auteurs : Redouane Rebia, Auteur ; Abedenacer Boudah, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 117 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : Diabète sucré Dépression Mélatonine Fluoxétine Désordres métaboliques Stress oxydatif Diabetes mellitus Depression Melatonin Fluoxetine Metabolic disorders Oxidative stress ??? ?????? ??????????? ?????????? ????? ?????? ??????? ???????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The chronic hyperglycemia is accompanied by excessive production of free radicals by
various mechanisms. This is an indicator of the oxidative stress that is involved in the
worsening of chronic complications related to diabetes mellitus. The objective of this study is
to evaluate the beneficial effects of melatonin and fluoxetine on metabolic disorders induced
by diabetes, as well as on the parameters of oxidative stress that could play a causal role in the
appearance of depressive behavior in rats rendered diabetic following an intraperitoneal
injection of streptozotocin (60 mg / kg). After 72 hours, the diabetic rats were treated with
melatonin and fluoxetine for 4 weeks. Behavioral tests such as the elevated plus maze (EPM),
the open field (OFT) and forced swimming (FST) were performed during the last week of
treatment to verify the antidepressant and anxiolytic effects of these two drugs.
At the end of this experiment, blood samples were taken for the determination of some
biochemical parameters, as well as the determination of oxidative stress markers in
erythrocytes, prefrontal cortex and hippocampus.
Our results showed that treatment with melatonin and fluoxetine significantly improved
anxiety behavior in diabetic rats during EPM and OFT, the same treatment was able to
reverse depressive behavior in the FST.
It was also noted that both treatments exert a regulating effect on blood glucose and lipid
profile parameters, thus these two drugs decrease erythrocyte lipoperoxidation and improve
the antioxidant status in the cerebral structures involved in the pathophysiology of depression
However, all these results clearly show that melatonin is endowed with a more potent
antioxidant activity than that of fluoxetine, and thus it would have an antidepressant effect.Diplôme : Doctorat En ligne : ../theses/biologie/REB7079.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10522 Corrélations entre le comportement dépressif, le profil lipidique et les paramètres du stress oxydatif au cours du diabète expérimental : Effet protecteur de la mélatonine et de la fluoxétine [texte imprimé] / Redouane Rebia, Auteur ; Abedenacer Boudah, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2017 . - 117 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Diabète sucré Dépression Mélatonine Fluoxétine Désordres métaboliques Stress oxydatif Diabetes mellitus Depression Melatonin Fluoxetine Metabolic disorders Oxidative stress ??? ?????? ??????????? ?????????? ????? ?????? ??????? ???????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The chronic hyperglycemia is accompanied by excessive production of free radicals by
various mechanisms. This is an indicator of the oxidative stress that is involved in the
worsening of chronic complications related to diabetes mellitus. The objective of this study is
to evaluate the beneficial effects of melatonin and fluoxetine on metabolic disorders induced
by diabetes, as well as on the parameters of oxidative stress that could play a causal role in the
appearance of depressive behavior in rats rendered diabetic following an intraperitoneal
injection of streptozotocin (60 mg / kg). After 72 hours, the diabetic rats were treated with
melatonin and fluoxetine for 4 weeks. Behavioral tests such as the elevated plus maze (EPM),
the open field (OFT) and forced swimming (FST) were performed during the last week of
treatment to verify the antidepressant and anxiolytic effects of these two drugs.
At the end of this experiment, blood samples were taken for the determination of some
biochemical parameters, as well as the determination of oxidative stress markers in
erythrocytes, prefrontal cortex and hippocampus.
Our results showed that treatment with melatonin and fluoxetine significantly improved
anxiety behavior in diabetic rats during EPM and OFT, the same treatment was able to
reverse depressive behavior in the FST.
It was also noted that both treatments exert a regulating effect on blood glucose and lipid
profile parameters, thus these two drugs decrease erythrocyte lipoperoxidation and improve
the antioxidant status in the cerebral structures involved in the pathophysiology of depression
However, all these results clearly show that melatonin is endowed with a more potent
antioxidant activity than that of fluoxetine, and thus it would have an antidepressant effect.Diplôme : Doctorat En ligne : ../theses/biologie/REB7079.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10522 Exemplaires
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Titre : Neurodegeneration, inflammation, oxidative stress and behavioral deficit following bilateral short term adrenalectomy in the nervous system of albino Wistar rats Type de document : texte imprimé Auteurs : Naserddine Hamadi, Auteur ; Fatima Khelifi Touhami, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 194 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Anglais (eng) Tags : surrénalectomie Hippocampe Neuroinflammation neurodégénérescence stress oxydatif Adrenalectomy Hippocampus Neurodegeneration Oxidative stress ??????? ????? ??????? ?????? ???????? ????? ??? ??????? ??????? ??????? ???????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Bilateral adrenalectomy has been shown to damage the hippocampal neurons. Although the effects of long-term adrenalectomy have
been studied extensively there are few publications on the effects of short-term adrenalectomy. In the present study we aimed to investigate
the effects of short-term bilateral adrenalectomy on the levels of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α; the response of
microglia and astrocytes to neuronal cell death animal behavior as well as oxidative stress markers GSH, SOD and MDA over the course of
time (4h, 24h, 3days, 1week and 2weeks) in the hippocampus of Wistar rats.
Our results showed a transient significant elevation of pro-inflammatory cytokines IL-1β and IL-6 from four hours to three days in
the adrenalectomized compared to sham operated rats. After one week, the elevation of both cytokines returns to the sham levels.
Surprisingly, TNF-α levels were significantly elevated at four hours only in adrenalectomized compared to sham operated rats. The
occurrence of neuronal cell death in the hippocampus following adrenalectomy was confirmed by Fluoro-Jade B staining. Our results showed
a time dependent increase in degenerated neurons in the dorsal blade of the dentate gyrus from three days to two weeks after adrenalectomy.
Our results revealed an early activation of microglia on day three whereas activation of astroglia in the hippocampus was observed at one
week postoperatively. A progression of microglia and astroglia activation all over the dentate gyrus and their appearance for the first time in
CA3 of adrenalectomized rats hippocampi compared to sham operated was seen after two weeks of surgery. Quantitative analysis revealed a
significant increase in the number of microglia (3, 7 and 14 days) and astrocytes (7 and 14 days) of ADX compared to sham operated rats. Our
study revealed no major signs of oxidative stress until two weeks after adrenalectomy when a significant decrease of GSH levels and SOD
activity as well as an increase in MDA levels were found in adrenalectomized compared to sham rats. In the current study we used passive
avoidance test to evaluate the cognitive functions of the ADX rats, we have found that the removal of the adrenal gland caused a
behavioral deficit in the adrenalectomized rats compared to the sham over the time (3, 7 and 14 days).
Our study showed an early increase in the pro-inflammatory cytokines followed by neurodegeneration and activation of glial
cells as well as oxidative stress. Taking these findings together it could be speculated that the early inflammatory components might
contribute to the initiation of the biological cascade responsible for subsequent neuronal death in the current neurodegenerative animal model.
These findings suggest that inflammatory mechanisms precede neurodegeneration and glial activation
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/HAM7230.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10797 Neurodegeneration, inflammation, oxidative stress and behavioral deficit following bilateral short term adrenalectomy in the nervous system of albino Wistar rats [texte imprimé] / Naserddine Hamadi, Auteur ; Fatima Khelifi Touhami, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2018 . - 194 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Anglais (eng)
Tags : surrénalectomie Hippocampe Neuroinflammation neurodégénérescence stress oxydatif Adrenalectomy Hippocampus Neurodegeneration Oxidative stress ??????? ????? ??????? ?????? ???????? ????? ??? ??????? ??????? ??????? ???????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : Bilateral adrenalectomy has been shown to damage the hippocampal neurons. Although the effects of long-term adrenalectomy have
been studied extensively there are few publications on the effects of short-term adrenalectomy. In the present study we aimed to investigate
the effects of short-term bilateral adrenalectomy on the levels of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α; the response of
microglia and astrocytes to neuronal cell death animal behavior as well as oxidative stress markers GSH, SOD and MDA over the course of
time (4h, 24h, 3days, 1week and 2weeks) in the hippocampus of Wistar rats.
Our results showed a transient significant elevation of pro-inflammatory cytokines IL-1β and IL-6 from four hours to three days in
the adrenalectomized compared to sham operated rats. After one week, the elevation of both cytokines returns to the sham levels.
Surprisingly, TNF-α levels were significantly elevated at four hours only in adrenalectomized compared to sham operated rats. The
occurrence of neuronal cell death in the hippocampus following adrenalectomy was confirmed by Fluoro-Jade B staining. Our results showed
a time dependent increase in degenerated neurons in the dorsal blade of the dentate gyrus from three days to two weeks after adrenalectomy.
Our results revealed an early activation of microglia on day three whereas activation of astroglia in the hippocampus was observed at one
week postoperatively. A progression of microglia and astroglia activation all over the dentate gyrus and their appearance for the first time in
CA3 of adrenalectomized rats hippocampi compared to sham operated was seen after two weeks of surgery. Quantitative analysis revealed a
significant increase in the number of microglia (3, 7 and 14 days) and astrocytes (7 and 14 days) of ADX compared to sham operated rats. Our
study revealed no major signs of oxidative stress until two weeks after adrenalectomy when a significant decrease of GSH levels and SOD
activity as well as an increase in MDA levels were found in adrenalectomized compared to sham rats. In the current study we used passive
avoidance test to evaluate the cognitive functions of the ADX rats, we have found that the removal of the adrenal gland caused a
behavioral deficit in the adrenalectomized rats compared to the sham over the time (3, 7 and 14 days).
Our study showed an early increase in the pro-inflammatory cytokines followed by neurodegeneration and activation of glial
cells as well as oxidative stress. Taking these findings together it could be speculated that the early inflammatory components might
contribute to the initiation of the biological cascade responsible for subsequent neuronal death in the current neurodegenerative animal model.
These findings suggest that inflammatory mechanisms precede neurodegeneration and glial activation
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/HAM7230.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10797 Exemplaires
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Titre : Le rôle de Thymelaea microphylla dans l’amélioration du stress oxydant et du taux de la métallothionéine induits par le cadmium dans le rein chez le rat. Type de document : texte imprimé Auteurs : Samira Laib, Auteur ; Korichi Lalaoui, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 94 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : chlorure de cadmium rein stress oxydatif Thymelaea microphylla métallothionéine cadmium chloride kidney oxidative stress metallothionein ????? ????????? ?????? ???????? ???????? Thymelaea microphylla????????????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present investigation was performed to evaluate the protective effect and the antioxidant power of Thymelaea microphylla against cadmium chloride induced oxidative stress and renal toxicity in Wistar rats. First, antioxidant and radical scavenging effect of Thymelaea microphylla was evaluated in vitro with 2,2- diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radicals assays. Then, Wistar rats were subjected to oral pretreatment with n-butanolic
extract of Thymelaea microphylla (BETM) (200 mg/kg) against the renal toxicity induced by oral administration of cadmium chloride CdCl2 (5 mg/kg) for 28 days, vitamin E (250 mg/Kg) was used similarly as standard. Efficiency of Thymelaea microphylla against the renal toxicity was evaluated in terms of biochemical estimation of renal function parameters, antioxidant enzymes activities, metallothionein levels and histopathological modifications. Thymelaea
microphylla anti-inflammatory effect was evaluated by carrageenan (1%) induced rat paw edema. Thymelaea microphylla revealed a high scavenging ability of DPPH and OH radicals.
Also, Thymelaea microphylla (200 mg/Kg) pretreatment prevented deteriorative effects caused by cadmium chloride through a protective mechanism that included significant decrease in the levels of Blood Urea Nitrogen, creatinine, gamma-glutamyltranspeptidase (GGT), potassium,sodium, Myeloperoxidase, Nitric Oxide and Thiobarbituric Acid Reactive Species as well as by restoration of antioxidant enzymes in the cytosol, metallothionein levels and
histopathological changes induced by cadmium. Additionally, Thymelaea microphylla revealed an important anti-inflammatory effect reducing carrageenan induced paw edema. This study proved the antioxidant and protective effect of Thymelaea microphylla against cadmium chloride induced oxidative stress and kidney injury.
Diplôme : Doctorat En ligne : ../theses/biologie/LAI7250.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10842 Le rôle de Thymelaea microphylla dans l’amélioration du stress oxydant et du taux de la métallothionéine induits par le cadmium dans le rein chez le rat. [texte imprimé] / Samira Laib, Auteur ; Korichi Lalaoui, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2018 . - 94 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : chlorure de cadmium rein stress oxydatif Thymelaea microphylla métallothionéine cadmium chloride kidney oxidative stress metallothionein ????? ????????? ?????? ???????? ???????? Thymelaea microphylla????????????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present investigation was performed to evaluate the protective effect and the antioxidant power of Thymelaea microphylla against cadmium chloride induced oxidative stress and renal toxicity in Wistar rats. First, antioxidant and radical scavenging effect of Thymelaea microphylla was evaluated in vitro with 2,2- diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radicals assays. Then, Wistar rats were subjected to oral pretreatment with n-butanolic
extract of Thymelaea microphylla (BETM) (200 mg/kg) against the renal toxicity induced by oral administration of cadmium chloride CdCl2 (5 mg/kg) for 28 days, vitamin E (250 mg/Kg) was used similarly as standard. Efficiency of Thymelaea microphylla against the renal toxicity was evaluated in terms of biochemical estimation of renal function parameters, antioxidant enzymes activities, metallothionein levels and histopathological modifications. Thymelaea
microphylla anti-inflammatory effect was evaluated by carrageenan (1%) induced rat paw edema. Thymelaea microphylla revealed a high scavenging ability of DPPH and OH radicals.
Also, Thymelaea microphylla (200 mg/Kg) pretreatment prevented deteriorative effects caused by cadmium chloride through a protective mechanism that included significant decrease in the levels of Blood Urea Nitrogen, creatinine, gamma-glutamyltranspeptidase (GGT), potassium,sodium, Myeloperoxidase, Nitric Oxide and Thiobarbituric Acid Reactive Species as well as by restoration of antioxidant enzymes in the cytosol, metallothionein levels and
histopathological changes induced by cadmium. Additionally, Thymelaea microphylla revealed an important anti-inflammatory effect reducing carrageenan induced paw edema. This study proved the antioxidant and protective effect of Thymelaea microphylla against cadmium chloride induced oxidative stress and kidney injury.
Diplôme : Doctorat En ligne : ../theses/biologie/LAI7250.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10842 Exemplaires
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Titre : L’effet protecteur des antioxydants naturels dans l’intoxication du mercure chez le rat Albinos Wistar : (Aspects biochimiques, immunologiques et histologiques). Type de document : texte imprimé Auteurs : Ahlem Bahi, Auteur ; Youcef Necib, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2015 Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : Mercure Huile d’argan Sélénium Stress oxydatif Argania Spinosa antioxydants Mercury Argan Oil Selenium Oxidative Stress antioxidants ????????? ??????? ???????? ??? ??????? ?????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present study aimed to evaluate the protective effect of argan oil and selenium against oxidative stress induced by mercury. For this reason, a phytochemical study of antioxidant tested in vitro was performed on argan oil to estimate its expected effect. The results showed the presence of phenolic compounds in a very high concentration. The evaluation of power scavenger argan oil is more active with IC50 = 13.33 ± 1.3, and in vivo on Albino Wistar rats were treated with mercury alone or combined with argan oil and selenium, for a period of three weeks, the blood is then withdrawn and the rats are sacrificed the various parameters are determined. From the analysis of our results, we observed a significant reduction in weight gain during treatment with mercury alone or combined with argan oil and selenium, but no clear change in the relative weight of liver, kidney or spleen was obtained. The results also showed significant changes in biochemical parameters, immunological and haematological. Characterized primarily by a significant increase in serum glucose, urea, creatinine, uric acid, total and direct bilirubin, total lipids, cholesterol, triglyceride, conjugated diene and the enzymatic activity of AST, ALT, LDH and PAL .dropoff window Whereas serum concentrations of albumin and total protein were reduced in treated rats compared to mercury control animals. Results also show immunological and haematological toxicity especially among the treated groups the pure mercury, an increase in serum IL-1, IL-6 and TNF and elevated white blood cell count, decreased red blood cell levels, hemoglobin concentration the percentage of hematocrit and phagocytic activity during treatment with mercury. Thus the treatment of the rats with the mercury causes a reduction in the plasma antioxidant capacity characterized by decrease in the serum concentration of vitamin E and C and rate of DPPH and thiols. We also observed that the results obtained highlight the detoxifier potential expressed by the decrease in erythrocyte glutathione levels, liver and kidney, metallothionein, the enzymatic activity of the antioxidant system SOD, GPx, GST and catalase and increased in lipid peroxidation expressed by the high levels of plasma MDA, erythrocyte, liver and kidney. Also the results clearly showed substantive alterations of tissue studies in comparison with the control, with hepatocyte cytolysis, hepatic necrosis and tubular degeneration with necrosis of the kidneys. Furthermore supplementation argan oil and selenium changed the toxic effects of mercury and decreased histological alterations and showed that both antioxidants are effective against oxidative stress induced by mercury
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BAH6696.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9928 L’effet protecteur des antioxydants naturels dans l’intoxication du mercure chez le rat Albinos Wistar : (Aspects biochimiques, immunologiques et histologiques). [texte imprimé] / Ahlem Bahi, Auteur ; Youcef Necib, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2015 . - ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Mercure Huile d’argan Sélénium Stress oxydatif Argania Spinosa antioxydants Mercury Argan Oil Selenium Oxidative Stress antioxidants ????????? ??????? ???????? ??? ??????? ?????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present study aimed to evaluate the protective effect of argan oil and selenium against oxidative stress induced by mercury. For this reason, a phytochemical study of antioxidant tested in vitro was performed on argan oil to estimate its expected effect. The results showed the presence of phenolic compounds in a very high concentration. The evaluation of power scavenger argan oil is more active with IC50 = 13.33 ± 1.3, and in vivo on Albino Wistar rats were treated with mercury alone or combined with argan oil and selenium, for a period of three weeks, the blood is then withdrawn and the rats are sacrificed the various parameters are determined. From the analysis of our results, we observed a significant reduction in weight gain during treatment with mercury alone or combined with argan oil and selenium, but no clear change in the relative weight of liver, kidney or spleen was obtained. The results also showed significant changes in biochemical parameters, immunological and haematological. Characterized primarily by a significant increase in serum glucose, urea, creatinine, uric acid, total and direct bilirubin, total lipids, cholesterol, triglyceride, conjugated diene and the enzymatic activity of AST, ALT, LDH and PAL .dropoff window Whereas serum concentrations of albumin and total protein were reduced in treated rats compared to mercury control animals. Results also show immunological and haematological toxicity especially among the treated groups the pure mercury, an increase in serum IL-1, IL-6 and TNF and elevated white blood cell count, decreased red blood cell levels, hemoglobin concentration the percentage of hematocrit and phagocytic activity during treatment with mercury. Thus the treatment of the rats with the mercury causes a reduction in the plasma antioxidant capacity characterized by decrease in the serum concentration of vitamin E and C and rate of DPPH and thiols. We also observed that the results obtained highlight the detoxifier potential expressed by the decrease in erythrocyte glutathione levels, liver and kidney, metallothionein, the enzymatic activity of the antioxidant system SOD, GPx, GST and catalase and increased in lipid peroxidation expressed by the high levels of plasma MDA, erythrocyte, liver and kidney. Also the results clearly showed substantive alterations of tissue studies in comparison with the control, with hepatocyte cytolysis, hepatic necrosis and tubular degeneration with necrosis of the kidneys. Furthermore supplementation argan oil and selenium changed the toxic effects of mercury and decreased histological alterations and showed that both antioxidants are effective against oxidative stress induced by mercury
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BAH6696.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9928 Exemplaires
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Titre : Etude analytique et épidémiologique de la toxicité des pesticides utilisés dans l’Est Algérien. Type de document : texte imprimé Auteurs : Awatif Boumaza, Auteur ; Korichi Lalaoui, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 99 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : Exposition aux pesticides génotoxicité biosurveillance stress oxydatif hématotoxicité troubles biochimiques pesticides exposure genotoxicity biomonitoring oxidative stress hematotoxicity biochemical disturbances Index. décimale : 570 Sciences de la vie. Biologie Résumé : Various pesticides have demonstrated hazardous, genotoxic and carcinogenic potential
via different mechanisms, among of which oxidative stress. Until now, biomonitoring and
epidemiological studies have been carried out to assess toxic and genotoxic risk to exposed
human populations. In this sense, the present study was carried out to determine cytotoxic and
genotoxic effects of some pesticides used in Algeria. First, six pesticides have been tested for
their potential genotoxicity using micronucleus test and chromosome aberration test in
cultured human lymphocytes, and Allium cepa test. Results of both types of tests, in vitro and
in situ, showed positive cytotoxic and genotoxic effects with the different tested pesticides. In
the second part of the present study, a population of pesticide-exposed farmers has been
monitored for possible health disturbances. Here, we focused on people exposed to complex
mixtures of pesticides. The exposure of farm workers from Constantine to these hazardous
chemicals was assessed and cytogenetic alterations were evaluated using blood lymphocytes
micronucleus assay, chromosome aberration assay, hematological and lipid parameters,
Butyrylcholinesterase (BChE) activity and oxidative stress enzymes and biomarkers involved
in the pathogenesis of pesticides. The group of farm workers exposed to different types of
pesticides was evaluated and compared to matched non-exposed control group. A significant
increase was observed in the frequencies of micronuclei, and chromosome aberrations in
blood lymphocytes in the exposed groups (n = 21) compared to controls (n = 21). Some
differences were detected regarding the hematological parameters, lipid profile and BChE
activity. A significant difference was observed regarding oxidative stress parameters and
biochemical parameters. These results suggest possible disrupting effect and genotoxic harms
of pesticides.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BOU7075.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10526 Etude analytique et épidémiologique de la toxicité des pesticides utilisés dans l’Est Algérien. [texte imprimé] / Awatif Boumaza, Auteur ; Korichi Lalaoui, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2017 . - 99 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Exposition aux pesticides génotoxicité biosurveillance stress oxydatif hématotoxicité troubles biochimiques pesticides exposure genotoxicity biomonitoring oxidative stress hematotoxicity biochemical disturbances Index. décimale : 570 Sciences de la vie. Biologie Résumé : Various pesticides have demonstrated hazardous, genotoxic and carcinogenic potential
via different mechanisms, among of which oxidative stress. Until now, biomonitoring and
epidemiological studies have been carried out to assess toxic and genotoxic risk to exposed
human populations. In this sense, the present study was carried out to determine cytotoxic and
genotoxic effects of some pesticides used in Algeria. First, six pesticides have been tested for
their potential genotoxicity using micronucleus test and chromosome aberration test in
cultured human lymphocytes, and Allium cepa test. Results of both types of tests, in vitro and
in situ, showed positive cytotoxic and genotoxic effects with the different tested pesticides. In
the second part of the present study, a population of pesticide-exposed farmers has been
monitored for possible health disturbances. Here, we focused on people exposed to complex
mixtures of pesticides. The exposure of farm workers from Constantine to these hazardous
chemicals was assessed and cytogenetic alterations were evaluated using blood lymphocytes
micronucleus assay, chromosome aberration assay, hematological and lipid parameters,
Butyrylcholinesterase (BChE) activity and oxidative stress enzymes and biomarkers involved
in the pathogenesis of pesticides. The group of farm workers exposed to different types of
pesticides was evaluated and compared to matched non-exposed control group. A significant
increase was observed in the frequencies of micronuclei, and chromosome aberrations in
blood lymphocytes in the exposed groups (n = 21) compared to controls (n = 21). Some
differences were detected regarding the hematological parameters, lipid profile and BChE
activity. A significant difference was observed regarding oxidative stress parameters and
biochemical parameters. These results suggest possible disrupting effect and genotoxic harms
of pesticides.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BOU7075.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10526 Exemplaires
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