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Titre : Etude des enzymes du stress oxydatif chez le blé dur (Triticum durum Desf.) : caractérisation biochimique et moléculaire. Type de document : texte imprimé Auteurs : Karima Bouchemal, Auteur ; Abdelhamid Djekoun, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 172 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Blé dur stress hydrique antioxydants isoenzymes stress oxydatif peroxydase catalase superoxyde dismutase expression génique Durum wheat water stress antioxidants oxydative stress peroxidase superoxide dismutase gene expression القمح الصلب الإجهاد المائي مضادات الأكسدة الإنزيمات المتشابهة الإجهاد التأكسدي التعبير الوراثيperoxydase Index. décimale : 570 Sciences de la vie. Biologie Résumé : Plant drought tolerance requires the activation of complex metabolic including antioxidative pathways, especially reactive oxygen species (ROS) scavenging enzymatic systems. In this study, 10 durum wheat genotypes (Triticum durum Desf.) were evaluated under drought stress conditions, in order to highlight the correlations between genetic diversity identified through enzymatic markers : superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and water stress tolerance properties. Firstly, in the first assay, we characterized the impact of oxidative stress and assessed the performance of different cultivars of durum wheat under drought, heat and paraquat stress. The activities of enzymatic antioxidants SOD, CAT, GPOX and oxidative biomarkers were measured : lipid peroxidation, H2O2 content, membrane stability, and total chlorophyll content. In addition, a correlation analysis between antioxidant enzymes, oxidative markers and physiological parameters related to water status was performed. Results indicated that the 3 treatments decreased membrane stability, chlorophyll content and increased lipid peroxidation, H2O2 content and activities of antioxidant enzymes.However, there were significant differences between wheat cultivars in amounts of decrease or increase in the measured traits. Correlation analysis between biochemical and physiological parameters revealed the singularity of SOD enzyme, which is particularly related to the relative water content, stomatal conductance and total chlorophyll content, The second part of this thesis was devoted to the study of the genetic variability of the three foliar enzymatic systems which allowed us to refine the results of the biochemical and physiological analysis as well as to highlight the influence of water stress on the metabolism of these enzymes in wheat plants grown hydroponically and treated with PEG 6000. An approach based on native PAGE has been developed to evaluate whether the observed overall variations, resulted from qualitative (presence/absence) or quantitative variations specific to certain isoenzymes. Marked differences were detected in the profiles of the studied 3 enzymatic systems studied. Electrophoretic analysis showed the induction of new isoforms and an increase in their intensity in durum wheat cultivars under water stress. Finally, the study of the expression of genes coding for these enzymes, in leaf cells of durum wheat was conducted through RealTime quantitative RT-PCR. Expression levels of 3 genes coding for CAT (TdCAT1, CATA, CAT3), 3 genes coding for POX (Pox3, TaPrx107, TaPrx115) and 2 genes coding for SOD (TdMnSOD, SOD1.1) were differently affected by drought. A variation in transcript accumulation was observed in response to water stress. This molecular approach identified the genes CATA, CAT3, TdMnSOD and SOD1.1 as candidate genes for potential genetic engineering targets and research for molecular markers as a selection tool for improving the tolerance of durum wheat to water stress. Diplôme : Doctorat En ligne : ../theses/biologie/BOU7398.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11048 Etude des enzymes du stress oxydatif chez le blé dur (Triticum durum Desf.) : caractérisation biochimique et moléculaire. [texte imprimé] / Karima Bouchemal, Auteur ; Abdelhamid Djekoun, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 172 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Blé dur stress hydrique antioxydants isoenzymes stress oxydatif peroxydase catalase superoxyde dismutase expression génique Durum wheat water stress antioxidants oxydative stress peroxidase superoxide dismutase gene expression القمح الصلب الإجهاد المائي مضادات الأكسدة الإنزيمات المتشابهة الإجهاد التأكسدي التعبير الوراثيperoxydase Index. décimale : 570 Sciences de la vie. Biologie Résumé : Plant drought tolerance requires the activation of complex metabolic including antioxidative pathways, especially reactive oxygen species (ROS) scavenging enzymatic systems. In this study, 10 durum wheat genotypes (Triticum durum Desf.) were evaluated under drought stress conditions, in order to highlight the correlations between genetic diversity identified through enzymatic markers : superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and water stress tolerance properties. Firstly, in the first assay, we characterized the impact of oxidative stress and assessed the performance of different cultivars of durum wheat under drought, heat and paraquat stress. The activities of enzymatic antioxidants SOD, CAT, GPOX and oxidative biomarkers were measured : lipid peroxidation, H2O2 content, membrane stability, and total chlorophyll content. In addition, a correlation analysis between antioxidant enzymes, oxidative markers and physiological parameters related to water status was performed. Results indicated that the 3 treatments decreased membrane stability, chlorophyll content and increased lipid peroxidation, H2O2 content and activities of antioxidant enzymes.However, there were significant differences between wheat cultivars in amounts of decrease or increase in the measured traits. Correlation analysis between biochemical and physiological parameters revealed the singularity of SOD enzyme, which is particularly related to the relative water content, stomatal conductance and total chlorophyll content, The second part of this thesis was devoted to the study of the genetic variability of the three foliar enzymatic systems which allowed us to refine the results of the biochemical and physiological analysis as well as to highlight the influence of water stress on the metabolism of these enzymes in wheat plants grown hydroponically and treated with PEG 6000. An approach based on native PAGE has been developed to evaluate whether the observed overall variations, resulted from qualitative (presence/absence) or quantitative variations specific to certain isoenzymes. Marked differences were detected in the profiles of the studied 3 enzymatic systems studied. Electrophoretic analysis showed the induction of new isoforms and an increase in their intensity in durum wheat cultivars under water stress. Finally, the study of the expression of genes coding for these enzymes, in leaf cells of durum wheat was conducted through RealTime quantitative RT-PCR. Expression levels of 3 genes coding for CAT (TdCAT1, CATA, CAT3), 3 genes coding for POX (Pox3, TaPrx107, TaPrx115) and 2 genes coding for SOD (TdMnSOD, SOD1.1) were differently affected by drought. A variation in transcript accumulation was observed in response to water stress. This molecular approach identified the genes CATA, CAT3, TdMnSOD and SOD1.1 as candidate genes for potential genetic engineering targets and research for molecular markers as a selection tool for improving the tolerance of durum wheat to water stress. Diplôme : Doctorat En ligne : ../theses/biologie/BOU7398.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11048 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/7398 BOU/7398 Thèse Bibliothèque principale Thèses Disponible Le rôle de Thymelaea microphylla dans l’amélioration du stress oxydant et du taux de la métallothionéine induits par le cadmium dans le rein chez le rat. / Samira Laib
Titre : Le rôle de Thymelaea microphylla dans l’amélioration du stress oxydant et du taux de la métallothionéine induits par le cadmium dans le rein chez le rat. Type de document : texte imprimé Auteurs : Samira Laib, Auteur ; Korichi Lalaoui, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 94 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : chlorure de cadmium rein stress oxydatif Thymelaea microphylla métallothionéine cadmium chloride kidney oxidative stress metallothionein لوريد الكادميوم الكلية والإجهاد التأكسدي Thymelaea microphyllaميتالوثيونيين Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present investigation was performed to evaluate the protective effect and the antioxidant power of Thymelaea microphylla against cadmium chloride induced oxidative stress and renal toxicity in Wistar rats. First, antioxidant and radical scavenging effect of Thymelaea microphylla was evaluated in vitro with 2,2- diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radicals assays. Then, Wistar rats were subjected to oral pretreatment with n-butanolic
extract of Thymelaea microphylla (BETM) (200 mg/kg) against the renal toxicity induced by oral administration of cadmium chloride CdCl2 (5 mg/kg) for 28 days, vitamin E (250 mg/Kg) was used similarly as standard. Efficiency of Thymelaea microphylla against the renal toxicity was evaluated in terms of biochemical estimation of renal function parameters, antioxidant enzymes activities, metallothionein levels and histopathological modifications. Thymelaea
microphylla anti-inflammatory effect was evaluated by carrageenan (1%) induced rat paw edema. Thymelaea microphylla revealed a high scavenging ability of DPPH and OH radicals.
Also, Thymelaea microphylla (200 mg/Kg) pretreatment prevented deteriorative effects caused by cadmium chloride through a protective mechanism that included significant decrease in the levels of Blood Urea Nitrogen, creatinine, gamma-glutamyltranspeptidase (GGT), potassium,sodium, Myeloperoxidase, Nitric Oxide and Thiobarbituric Acid Reactive Species as well as by restoration of antioxidant enzymes in the cytosol, metallothionein levels and
histopathological changes induced by cadmium. Additionally, Thymelaea microphylla revealed an important anti-inflammatory effect reducing carrageenan induced paw edema. This study proved the antioxidant and protective effect of Thymelaea microphylla against cadmium chloride induced oxidative stress and kidney injury.
Diplôme : Doctorat En ligne : ../theses/biologie/LAI7250.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10842 Le rôle de Thymelaea microphylla dans l’amélioration du stress oxydant et du taux de la métallothionéine induits par le cadmium dans le rein chez le rat. [texte imprimé] / Samira Laib, Auteur ; Korichi Lalaoui, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 94 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : chlorure de cadmium rein stress oxydatif Thymelaea microphylla métallothionéine cadmium chloride kidney oxidative stress metallothionein لوريد الكادميوم الكلية والإجهاد التأكسدي Thymelaea microphyllaميتالوثيونيين Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present investigation was performed to evaluate the protective effect and the antioxidant power of Thymelaea microphylla against cadmium chloride induced oxidative stress and renal toxicity in Wistar rats. First, antioxidant and radical scavenging effect of Thymelaea microphylla was evaluated in vitro with 2,2- diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radicals assays. Then, Wistar rats were subjected to oral pretreatment with n-butanolic
extract of Thymelaea microphylla (BETM) (200 mg/kg) against the renal toxicity induced by oral administration of cadmium chloride CdCl2 (5 mg/kg) for 28 days, vitamin E (250 mg/Kg) was used similarly as standard. Efficiency of Thymelaea microphylla against the renal toxicity was evaluated in terms of biochemical estimation of renal function parameters, antioxidant enzymes activities, metallothionein levels and histopathological modifications. Thymelaea
microphylla anti-inflammatory effect was evaluated by carrageenan (1%) induced rat paw edema. Thymelaea microphylla revealed a high scavenging ability of DPPH and OH radicals.
Also, Thymelaea microphylla (200 mg/Kg) pretreatment prevented deteriorative effects caused by cadmium chloride through a protective mechanism that included significant decrease in the levels of Blood Urea Nitrogen, creatinine, gamma-glutamyltranspeptidase (GGT), potassium,sodium, Myeloperoxidase, Nitric Oxide and Thiobarbituric Acid Reactive Species as well as by restoration of antioxidant enzymes in the cytosol, metallothionein levels and
histopathological changes induced by cadmium. Additionally, Thymelaea microphylla revealed an important anti-inflammatory effect reducing carrageenan induced paw edema. This study proved the antioxidant and protective effect of Thymelaea microphylla against cadmium chloride induced oxidative stress and kidney injury.
Diplôme : Doctorat En ligne : ../theses/biologie/LAI7250.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10842 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAI/7250 LAI/7250 Thèse Bibliothèque principale Thèses Disponible
Titre : L’accumulation de la proline sous déficit hydrique, synthèse et devenir après retour de l’arrosage chez les végétaux : Exemple des Triticum et Hordeum. Type de document : texte imprimé Auteurs : Sarra Merabta, Auteur ; Mostefa Benlaribi, Directeur de thèse Mention d'édition : 18/12/2019 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 164 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie et Ecologie Végétale:Biotechnologies, Biologie et Environnement Biotechnologies, Biologie et Environnement:Les bases Biologiques de la Production et Biodiversité Végétale Blé (Triticum) orge(Hordeum) stress hydrique Proline chlorophylle Stress oxydatif enzymes Wheat (Triticum) Barley (Hordeum) hydrous stress Chlorophyll Oxidative stress القمح ( )Triticumالشعير ( )Hordeumالاجهاد المائي البرولين الكلوروفيل الاجهاد
التأكسد الانزيماتIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
The objective of this work consists in evaluating the effect of a hydrous deficit created using a restriction of irrigation for a period of twenty (20) days on two durum wheat genotypes (Triticum durum Desf.): Gamgoum Rekham ( GGR) and Haurani; two genotypes of common wheat (Triticum aestivum L.): Florence Aurore 8193 (FA) and Mexipak and two genotypes of barley (Hordeum vulgare L.): Manel and Saida 183 and to follow the impact of this controlled stress after 24 hours (APR1) and one week (APR2), following the return of watering. A first experiment is carried out in order to compare the relation between Proline and chlorophyll, the application of prolonged hydric stress causes an accumulation of the content of proline in the leaves of the two kinds tested compared to that of their witnesses. However, this accumulation is in favor of the Triticum kind beside the Hordeum kind with a remarkable
diversity within the different varieties. As for the total chlorophyll composition of the organs studied, it evolves in a sawtooth pattern that does not lead to any indicative trend during the various treatments. After rehydration, there is a progressive decrease in proline content (APR1 and APR2), particularly at the two barley genotypes, and any change concerning the chlorophyll content. One second experimentation is carried out on the same genotypes with the same hydric treatments but this time to determine the influence of the oxydative stress produced by the dryness on the antioxydant metabolism. We were particularly interested in the activity of the superoxyde dismutase (SOD), malonedialdéhydes (MDA) content, catalase (CAT), peroxidases content (POD), as well as the ascorbate peroxidases (APX), and the content of proteins, aiming to characterize varieties according to their defense mechanisms against the effects of free radicals induced by water stress more precisely during the most critical stage and more affected by this Lack (heading stage) and to evaluate the impact of the oxydative
defensive system (Indicators of oxidation and antioxidant enzymes activities) on the synthesis and the degradation of the proline in order to identify the elements of the signaling pathways involved, and to evaluate the effect of this stress on the synthesis of this compound since the precursor molecules and its fate after the return of watering.Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/biologie/MER7611.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11447 L’accumulation de la proline sous déficit hydrique, synthèse et devenir après retour de l’arrosage chez les végétaux : Exemple des Triticum et Hordeum. [texte imprimé] / Sarra Merabta, Auteur ; Mostefa Benlaribi, Directeur de thèse . - 18/12/2019 . - جامعة الإخوة منتوري قسنطينة, 2019 . - 164 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie et Ecologie Végétale:Biotechnologies, Biologie et Environnement Biotechnologies, Biologie et Environnement:Les bases Biologiques de la Production et Biodiversité Végétale Blé (Triticum) orge(Hordeum) stress hydrique Proline chlorophylle Stress oxydatif enzymes Wheat (Triticum) Barley (Hordeum) hydrous stress Chlorophyll Oxidative stress القمح ( )Triticumالشعير ( )Hordeumالاجهاد المائي البرولين الكلوروفيل الاجهاد
التأكسد الانزيماتIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
The objective of this work consists in evaluating the effect of a hydrous deficit created using a restriction of irrigation for a period of twenty (20) days on two durum wheat genotypes (Triticum durum Desf.): Gamgoum Rekham ( GGR) and Haurani; two genotypes of common wheat (Triticum aestivum L.): Florence Aurore 8193 (FA) and Mexipak and two genotypes of barley (Hordeum vulgare L.): Manel and Saida 183 and to follow the impact of this controlled stress after 24 hours (APR1) and one week (APR2), following the return of watering. A first experiment is carried out in order to compare the relation between Proline and chlorophyll, the application of prolonged hydric stress causes an accumulation of the content of proline in the leaves of the two kinds tested compared to that of their witnesses. However, this accumulation is in favor of the Triticum kind beside the Hordeum kind with a remarkable
diversity within the different varieties. As for the total chlorophyll composition of the organs studied, it evolves in a sawtooth pattern that does not lead to any indicative trend during the various treatments. After rehydration, there is a progressive decrease in proline content (APR1 and APR2), particularly at the two barley genotypes, and any change concerning the chlorophyll content. One second experimentation is carried out on the same genotypes with the same hydric treatments but this time to determine the influence of the oxydative stress produced by the dryness on the antioxydant metabolism. We were particularly interested in the activity of the superoxyde dismutase (SOD), malonedialdéhydes (MDA) content, catalase (CAT), peroxidases content (POD), as well as the ascorbate peroxidases (APX), and the content of proteins, aiming to characterize varieties according to their defense mechanisms against the effects of free radicals induced by water stress more precisely during the most critical stage and more affected by this Lack (heading stage) and to evaluate the impact of the oxydative
defensive system (Indicators of oxidation and antioxidant enzymes activities) on the synthesis and the degradation of the proline in order to identify the elements of the signaling pathways involved, and to evaluate the effect of this stress on the synthesis of this compound since the precursor molecules and its fate after the return of watering.Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/biologie/MER7611.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11447 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MER/7611 MER/7611 Thèse Bibliothèque principale Thèses Disponible
Titre : Corrélations entre le comportement dépressif, le profil lipidique et les paramètres du stress oxydatif au cours du diabète expérimental : Effet protecteur de la mélatonine et de la fluoxétine Type de document : texte imprimé Auteurs : Redouane Rebai, Auteur ; Abedenacer Boudah, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 117 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Diabète sucré Dépression Mélatonine Fluoxétine Désordres métaboliques Stress oxydatif Diabetes mellitus Depression Melatonin Fluoxetine Metabolic disorders Oxidative stress داء السكري الميلاتونين الفلوكستين مستوى الدهون الإجهاد التأكسدي Index. décimale : 570 Sciences de la vie. Biologie Résumé : The chronic hyperglycemia is accompanied by excessive production of free radicals by
various mechanisms. This is an indicator of the oxidative stress that is involved in the
worsening of chronic complications related to diabetes mellitus. The objective of this study is
to evaluate the beneficial effects of melatonin and fluoxetine on metabolic disorders induced
by diabetes, as well as on the parameters of oxidative stress that could play a causal role in the
appearance of depressive behavior in rats rendered diabetic following an intraperitoneal
injection of streptozotocin (60 mg / kg). After 72 hours, the diabetic rats were treated with
melatonin and fluoxetine for 4 weeks. Behavioral tests such as the elevated plus maze (EPM),
the open field (OFT) and forced swimming (FST) were performed during the last week of
treatment to verify the antidepressant and anxiolytic effects of these two drugs.
At the end of this experiment, blood samples were taken for the determination of some
biochemical parameters, as well as the determination of oxidative stress markers in
erythrocytes, prefrontal cortex and hippocampus.
Our results showed that treatment with melatonin and fluoxetine significantly improved
anxiety behavior in diabetic rats during EPM and OFT, the same treatment was able to
reverse depressive behavior in the FST.
It was also noted that both treatments exert a regulating effect on blood glucose and lipid
profile parameters, thus these two drugs decrease erythrocyte lipoperoxidation and improve
the antioxidant status in the cerebral structures involved in the pathophysiology of depression
However, all these results clearly show that melatonin is endowed with a more potent
antioxidant activity than that of fluoxetine, and thus it would have an antidepressant effect.Diplôme : Doctorat En ligne : ../theses/biologie/REB7079.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10522 Corrélations entre le comportement dépressif, le profil lipidique et les paramètres du stress oxydatif au cours du diabète expérimental : Effet protecteur de la mélatonine et de la fluoxétine [texte imprimé] / Redouane Rebai, Auteur ; Abedenacer Boudah, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 117 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Diabète sucré Dépression Mélatonine Fluoxétine Désordres métaboliques Stress oxydatif Diabetes mellitus Depression Melatonin Fluoxetine Metabolic disorders Oxidative stress داء السكري الميلاتونين الفلوكستين مستوى الدهون الإجهاد التأكسدي Index. décimale : 570 Sciences de la vie. Biologie Résumé : The chronic hyperglycemia is accompanied by excessive production of free radicals by
various mechanisms. This is an indicator of the oxidative stress that is involved in the
worsening of chronic complications related to diabetes mellitus. The objective of this study is
to evaluate the beneficial effects of melatonin and fluoxetine on metabolic disorders induced
by diabetes, as well as on the parameters of oxidative stress that could play a causal role in the
appearance of depressive behavior in rats rendered diabetic following an intraperitoneal
injection of streptozotocin (60 mg / kg). After 72 hours, the diabetic rats were treated with
melatonin and fluoxetine for 4 weeks. Behavioral tests such as the elevated plus maze (EPM),
the open field (OFT) and forced swimming (FST) were performed during the last week of
treatment to verify the antidepressant and anxiolytic effects of these two drugs.
At the end of this experiment, blood samples were taken for the determination of some
biochemical parameters, as well as the determination of oxidative stress markers in
erythrocytes, prefrontal cortex and hippocampus.
Our results showed that treatment with melatonin and fluoxetine significantly improved
anxiety behavior in diabetic rats during EPM and OFT, the same treatment was able to
reverse depressive behavior in the FST.
It was also noted that both treatments exert a regulating effect on blood glucose and lipid
profile parameters, thus these two drugs decrease erythrocyte lipoperoxidation and improve
the antioxidant status in the cerebral structures involved in the pathophysiology of depression
However, all these results clearly show that melatonin is endowed with a more potent
antioxidant activity than that of fluoxetine, and thus it would have an antidepressant effect.Diplôme : Doctorat En ligne : ../theses/biologie/REB7079.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10522 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité REB/7079 REB/7079 Thèse Bibliothèque principale Thèses Disponible
Titre : Neurodegeneration, inflammation, oxidative stress and behavioral deficit following bilateral short term adrenalectomy in the nervous system of albino Wistar rats Type de document : texte imprimé Auteurs : Naserddine Hamadi, Auteur ; Fatima Khelifi Touhami, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 194 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Anglais (eng) Catégories : Français - Anglais
BiologieTags : surrénalectomie Hippocampe Neuroinflammation neurodégénérescence stress oxydatif Adrenalectomy Hippocampus Neurodegeneration Oxidative stress استئصال الغدة الكظرية الحصين التهابات عصبية تلف الخلايا العصبية الاجهاد التأكسدي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Bilateral adrenalectomy has been shown to damage the hippocampal neurons. Although the effects of long-term adrenalectomy have
been studied extensively there are few publications on the effects of short-term adrenalectomy. In the present study we aimed to investigate
the effects of short-term bilateral adrenalectomy on the levels of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α; the response of
microglia and astrocytes to neuronal cell death animal behavior as well as oxidative stress markers GSH, SOD and MDA over the course of
time (4h, 24h, 3days, 1week and 2weeks) in the hippocampus of Wistar rats.
Our results showed a transient significant elevation of pro-inflammatory cytokines IL-1β and IL-6 from four hours to three days in
the adrenalectomized compared to sham operated rats. After one week, the elevation of both cytokines returns to the sham levels.
Surprisingly, TNF-α levels were significantly elevated at four hours only in adrenalectomized compared to sham operated rats. The
occurrence of neuronal cell death in the hippocampus following adrenalectomy was confirmed by Fluoro-Jade B staining. Our results showed
a time dependent increase in degenerated neurons in the dorsal blade of the dentate gyrus from three days to two weeks after adrenalectomy.
Our results revealed an early activation of microglia on day three whereas activation of astroglia in the hippocampus was observed at one
week postoperatively. A progression of microglia and astroglia activation all over the dentate gyrus and their appearance for the first time in
CA3 of adrenalectomized rats hippocampi compared to sham operated was seen after two weeks of surgery. Quantitative analysis revealed a
significant increase in the number of microglia (3, 7 and 14 days) and astrocytes (7 and 14 days) of ADX compared to sham operated rats. Our
study revealed no major signs of oxidative stress until two weeks after adrenalectomy when a significant decrease of GSH levels and SOD
activity as well as an increase in MDA levels were found in adrenalectomized compared to sham rats. In the current study we used passive
avoidance test to evaluate the cognitive functions of the ADX rats, we have found that the removal of the adrenal gland caused a
behavioral deficit in the adrenalectomized rats compared to the sham over the time (3, 7 and 14 days).
Our study showed an early increase in the pro-inflammatory cytokines followed by neurodegeneration and activation of glial
cells as well as oxidative stress. Taking these findings together it could be speculated that the early inflammatory components might
contribute to the initiation of the biological cascade responsible for subsequent neuronal death in the current neurodegenerative animal model.
These findings suggest that inflammatory mechanisms precede neurodegeneration and glial activation
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/HAM7230.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10797 Neurodegeneration, inflammation, oxidative stress and behavioral deficit following bilateral short term adrenalectomy in the nervous system of albino Wistar rats [texte imprimé] / Naserddine Hamadi, Auteur ; Fatima Khelifi Touhami, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 194 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Anglais (eng)
Catégories : Français - Anglais
BiologieTags : surrénalectomie Hippocampe Neuroinflammation neurodégénérescence stress oxydatif Adrenalectomy Hippocampus Neurodegeneration Oxidative stress استئصال الغدة الكظرية الحصين التهابات عصبية تلف الخلايا العصبية الاجهاد التأكسدي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Bilateral adrenalectomy has been shown to damage the hippocampal neurons. Although the effects of long-term adrenalectomy have
been studied extensively there are few publications on the effects of short-term adrenalectomy. In the present study we aimed to investigate
the effects of short-term bilateral adrenalectomy on the levels of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α; the response of
microglia and astrocytes to neuronal cell death animal behavior as well as oxidative stress markers GSH, SOD and MDA over the course of
time (4h, 24h, 3days, 1week and 2weeks) in the hippocampus of Wistar rats.
Our results showed a transient significant elevation of pro-inflammatory cytokines IL-1β and IL-6 from four hours to three days in
the adrenalectomized compared to sham operated rats. After one week, the elevation of both cytokines returns to the sham levels.
Surprisingly, TNF-α levels were significantly elevated at four hours only in adrenalectomized compared to sham operated rats. The
occurrence of neuronal cell death in the hippocampus following adrenalectomy was confirmed by Fluoro-Jade B staining. Our results showed
a time dependent increase in degenerated neurons in the dorsal blade of the dentate gyrus from three days to two weeks after adrenalectomy.
Our results revealed an early activation of microglia on day three whereas activation of astroglia in the hippocampus was observed at one
week postoperatively. A progression of microglia and astroglia activation all over the dentate gyrus and their appearance for the first time in
CA3 of adrenalectomized rats hippocampi compared to sham operated was seen after two weeks of surgery. Quantitative analysis revealed a
significant increase in the number of microglia (3, 7 and 14 days) and astrocytes (7 and 14 days) of ADX compared to sham operated rats. Our
study revealed no major signs of oxidative stress until two weeks after adrenalectomy when a significant decrease of GSH levels and SOD
activity as well as an increase in MDA levels were found in adrenalectomized compared to sham rats. In the current study we used passive
avoidance test to evaluate the cognitive functions of the ADX rats, we have found that the removal of the adrenal gland caused a
behavioral deficit in the adrenalectomized rats compared to the sham over the time (3, 7 and 14 days).
Our study showed an early increase in the pro-inflammatory cytokines followed by neurodegeneration and activation of glial
cells as well as oxidative stress. Taking these findings together it could be speculated that the early inflammatory components might
contribute to the initiation of the biological cascade responsible for subsequent neuronal death in the current neurodegenerative animal model.
These findings suggest that inflammatory mechanisms precede neurodegeneration and glial activation
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Code-barres Cote Support Localisation Section Disponibilité HAM/7230 HAM/7230 Thèse Bibliothèque principale Thèses Disponible
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