| Titre : |
Propriétés antioxydantes et activité inhibitrice de la Xanthine oxydase des extraits de la plante médicinale Ajuga iva (L.) Schreber. |
| Type de document : |
texte imprimé |
| Auteurs : |
Moufida Adjadj, Auteur ; Abderrahmane Baghiani, Directeur de thèse |
| Editeur : |
جامعة الإخوة منتوري قسنطينة |
| Année de publication : |
2009 |
| Importance : |
96 f. |
| Format : |
30 cm. |
| Note générale : |
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD |
| Langues : |
Français (fre) |
| Catégories : |
Français - Anglais
Biologie
|
| Tags : |
Flavonoides Stress oxydatif Antioxydants Plantes médicinales Ajuga iva Effet scavenger DPPH Péroxydationlipidique Béta-caroèe/acide linoléique |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
This study was conducted to search for XO inhibitors from Ajuga iva shoot extracts
(AISE) traditionally used in folk medicine in Algeria. XO was purified from fresh bovine
milk with yields of ? ? ? ? ? mg / L, protein / flavine ratio of 5.15 and a single major band, on
SDS-PAGE, of approximately 150 KDa, indicating a high purity of the purified enzyme. The
specific activity of the purified enzyme was of 2055.05 nmol / min / mg protein. The analysis
of methanol (CrE), chloroform (ChE) and ethyl acetate (EaE) extracts, showed that the EaE
has significant amount of polyphenols (35.83 ± 0.69 mg EGA / g dry extract), followed by
ChE and CrE (30.58 ± 0.2 and 17.38 ± 0.29 mg EGA / g dry extract, respectively). The ChE
has the highest flavonoids content (9.64 ± 0.33 mg EQ / g dry extract), followed by EaE and
CrE with 7.72 ± 0.19 et 4.92 ± 0.30 mg EQ / g dry extract, respectively. The ChE showed the
highest inhibitory properties on the XO activity (IC50 = 0.134 ± 0.00032 mg / ml) and on the
cytochrome c reduction (IC50 = 0.23 ± 0.0017 mg / ml), followed by EaE with IC50 of XO
inhibitory activity and an IC50 of the inhibition of cyt c reduction of 0.185 ± 0.0019 and 1.046
± 0.0055 mg /ml, respectively. Finally, CrE had the lowest inhibitory effect on XO activity
and on cyt c reduction with 0.367 ± 0.0041 and 3.34 ± 0.018 mg / ml, respectively. The
antioxidant potential was confirmed with the non enzymatic methods, DPPH assay and β-
carotene / linoleic acid bleaching assay. The highest DPPH radical scavenging activity was
observed with EaE (IC50 = 228.01 ± 2.26 µg / ml), followed by CrE and ChE with IC50 of
415.06 ± 0.59 and 466.33 ± 3.20 µg / ml, respectively. The β-carotene / linoleic acid
bleaching assay revealed that AISE have a very important antioxidant activity. Results
showed that ChE has the highest antioxidant activity (95.64 ± 0.15 %), followed by CrE and
EaE with 87.97 ± 0.10 % and 72.30 ± 0.35 %, respectively. These results suggest that these
natural products could be used to treat lot of diseases, where inhibition of XO, free radical
scavenging activity and / or inhibition of lipid peroxidation are necessary. |
| Diplôme : |
Magistère |
| En ligne : |
../theses/biologie/ADJ5396.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=1800 |
Propriétés antioxydantes et activité inhibitrice de la Xanthine oxydase des extraits de la plante médicinale Ajuga iva (L.) Schreber. [texte imprimé] / Moufida Adjadj, Auteur ; Abderrahmane Baghiani, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2009 . - 96 f. ; 30 cm. 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français ( fre)
| Catégories : |
Français - Anglais
Biologie
|
| Tags : |
Flavonoides Stress oxydatif Antioxydants Plantes médicinales Ajuga iva Effet scavenger DPPH Péroxydationlipidique Béta-caroèe/acide linoléique |
| Index. décimale : |
570 Sciences de la vie. Biologie |
| Résumé : |
This study was conducted to search for XO inhibitors from Ajuga iva shoot extracts
(AISE) traditionally used in folk medicine in Algeria. XO was purified from fresh bovine
milk with yields of ? ? ? ? ? mg / L, protein / flavine ratio of 5.15 and a single major band, on
SDS-PAGE, of approximately 150 KDa, indicating a high purity of the purified enzyme. The
specific activity of the purified enzyme was of 2055.05 nmol / min / mg protein. The analysis
of methanol (CrE), chloroform (ChE) and ethyl acetate (EaE) extracts, showed that the EaE
has significant amount of polyphenols (35.83 ± 0.69 mg EGA / g dry extract), followed by
ChE and CrE (30.58 ± 0.2 and 17.38 ± 0.29 mg EGA / g dry extract, respectively). The ChE
has the highest flavonoids content (9.64 ± 0.33 mg EQ / g dry extract), followed by EaE and
CrE with 7.72 ± 0.19 et 4.92 ± 0.30 mg EQ / g dry extract, respectively. The ChE showed the
highest inhibitory properties on the XO activity (IC50 = 0.134 ± 0.00032 mg / ml) and on the
cytochrome c reduction (IC50 = 0.23 ± 0.0017 mg / ml), followed by EaE with IC50 of XO
inhibitory activity and an IC50 of the inhibition of cyt c reduction of 0.185 ± 0.0019 and 1.046
± 0.0055 mg /ml, respectively. Finally, CrE had the lowest inhibitory effect on XO activity
and on cyt c reduction with 0.367 ± 0.0041 and 3.34 ± 0.018 mg / ml, respectively. The
antioxidant potential was confirmed with the non enzymatic methods, DPPH assay and β-
carotene / linoleic acid bleaching assay. The highest DPPH radical scavenging activity was
observed with EaE (IC50 = 228.01 ± 2.26 µg / ml), followed by CrE and ChE with IC50 of
415.06 ± 0.59 and 466.33 ± 3.20 µg / ml, respectively. The β-carotene / linoleic acid
bleaching assay revealed that AISE have a very important antioxidant activity. Results
showed that ChE has the highest antioxidant activity (95.64 ± 0.15 %), followed by CrE and
EaE with 87.97 ± 0.10 % and 72.30 ± 0.35 %, respectively. These results suggest that these
natural products could be used to treat lot of diseases, where inhibition of XO, free radical
scavenging activity and / or inhibition of lipid peroxidation are necessary. |
| Diplôme : |
Magistère |
| En ligne : |
../theses/biologie/ADJ5396.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
index.php?lvl=notice_display&id=1800 |
|
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