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Partager le résultat de cette recherche Interroger des sources externesEffet de la saison de collecte sur la valeur nutritive, la production de méthane et de tannins condensés d’arbustes fourragers locaux. / Samir Medjekal
Titre : Effet de la saison de collecte sur la valeur nutritive, la production de méthane et de tannins condensés d’arbustes fourragers locaux. : Essai de contrôle in vitro de la méthanogénèse ruminale d’ovins par l’utilisation de plantes médicinales. Type de document : texte imprimé Auteurs : Samir Medjekal, Auteur ; H. Bousseboua, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 72 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Arbustes fourragers analyse chimique tannins condensés fermentescibilité in vitro digestibilité méthanogénèse plantes médicinales Fodder shrubs chemical analysis condensed tannins in vitro fermentation digestibility methanogenesis medicinal plants الشجيرات العلفية التحليل الكميائي التانين المكثف التخمر المخبري الهضم انتاج الميتان النباتات الطبية Index. décimale : 570 Sciences de la vie. Biologie Résumé : The main objectives of this thesis were to study, in a first part, the effect of season collection or harvest on the nutritional value, tannin content and methane production of three forage shrubs, Atriplex canesens, Astragalus and Calobota sahrae taken from an arid area of Algeria during three different seasons, through several aspects: chemical characterization, evaluating their potential nutritive value by measuring their fermentation and digestibility in vitro.
In a second part, a reduction test of ruminal methanogenesis in vitro by the use of
three medicinal plants selected according to their therapeutic characteristics in traditional medicine (Nigella sativa, Rosmarinus officinalis.L and Zingiber officinale), first , on ruminal fermentation parameters, gas production, methane and digestibility, and on the other hand, the impact of their use in the quantitative and qualitative production of volatile fatty acids.
Chemical analysis revealed that the collection season has a significant effect on all the chemical components of the three shrubs investigated herein, except on condensed tannins.
Atriplex canescens, has a very high crude protein content during winter that can reach 200 (g / kg DM). Regarding its content of cell wall constituents, they are very high in winter and spring, compared to values obtained for the summer. The same variations in chemical composition were recorded with Astragalus gombo, unlike Calobota sahrae which has very high values in summer compared to winter and spring. This is probably due to climate conditions in M'sila region, characterized by high temperatures and low rainfall.
From the chemical point of view, the temporal variations show that the season effect
on the different results obtained is significant. This is an effect of climate change associated with it. Indeed, we recorded a net variability for crude protein content and cell wall constituents. Despite the outstanding features of adaptation of spontaneous flora Sahara to harsh climatic conditions of their desert environment, it is clear that their survival, proliferation and their chemical compositions are highly dependent on climatic variations.
This study has practical implications for the development of agro-forestry technologies. The three plants studied show good potential as fodder shrubs for ecosystem rehabilitation projects in degraded arid and semi-arid rangelands.
Medicinal plants are a natural source of chemical molecules that can positively alter
rumen fermentation. In general, a high methane reduction was noted with Monensin and
Nigella sativa and no effect was observed with the other treatments. This study again
confirms that the Monensin significantly inhibits the production of CH4 in the rumen. In terms of Nigella sativa, it is widely known that this plant is very rich in lipids and sterols, which could be the cause of the reduction. Information in the literature with respect to antimethanogenic effects of certain plant species and plant secondary metabolites and on secondary metabolites in these species can be described as a major interest for the interpretation of such results and discuss future research to identify the chemical components responsible for antimethanogenic activity.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MED6921.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10273 Effet de la saison de collecte sur la valeur nutritive, la production de méthane et de tannins condensés d’arbustes fourragers locaux. : Essai de contrôle in vitro de la méthanogénèse ruminale d’ovins par l’utilisation de plantes médicinales. [texte imprimé] / Samir Medjekal, Auteur ; H. Bousseboua, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 72 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Arbustes fourragers analyse chimique tannins condensés fermentescibilité in vitro digestibilité méthanogénèse plantes médicinales Fodder shrubs chemical analysis condensed tannins in vitro fermentation digestibility methanogenesis medicinal plants الشجيرات العلفية التحليل الكميائي التانين المكثف التخمر المخبري الهضم انتاج الميتان النباتات الطبية Index. décimale : 570 Sciences de la vie. Biologie Résumé : The main objectives of this thesis were to study, in a first part, the effect of season collection or harvest on the nutritional value, tannin content and methane production of three forage shrubs, Atriplex canesens, Astragalus and Calobota sahrae taken from an arid area of Algeria during three different seasons, through several aspects: chemical characterization, evaluating their potential nutritive value by measuring their fermentation and digestibility in vitro.
In a second part, a reduction test of ruminal methanogenesis in vitro by the use of
three medicinal plants selected according to their therapeutic characteristics in traditional medicine (Nigella sativa, Rosmarinus officinalis.L and Zingiber officinale), first , on ruminal fermentation parameters, gas production, methane and digestibility, and on the other hand, the impact of their use in the quantitative and qualitative production of volatile fatty acids.
Chemical analysis revealed that the collection season has a significant effect on all the chemical components of the three shrubs investigated herein, except on condensed tannins.
Atriplex canescens, has a very high crude protein content during winter that can reach 200 (g / kg DM). Regarding its content of cell wall constituents, they are very high in winter and spring, compared to values obtained for the summer. The same variations in chemical composition were recorded with Astragalus gombo, unlike Calobota sahrae which has very high values in summer compared to winter and spring. This is probably due to climate conditions in M'sila region, characterized by high temperatures and low rainfall.
From the chemical point of view, the temporal variations show that the season effect
on the different results obtained is significant. This is an effect of climate change associated with it. Indeed, we recorded a net variability for crude protein content and cell wall constituents. Despite the outstanding features of adaptation of spontaneous flora Sahara to harsh climatic conditions of their desert environment, it is clear that their survival, proliferation and their chemical compositions are highly dependent on climatic variations.
This study has practical implications for the development of agro-forestry technologies. The three plants studied show good potential as fodder shrubs for ecosystem rehabilitation projects in degraded arid and semi-arid rangelands.
Medicinal plants are a natural source of chemical molecules that can positively alter
rumen fermentation. In general, a high methane reduction was noted with Monensin and
Nigella sativa and no effect was observed with the other treatments. This study again
confirms that the Monensin significantly inhibits the production of CH4 in the rumen. In terms of Nigella sativa, it is widely known that this plant is very rich in lipids and sterols, which could be the cause of the reduction. Information in the literature with respect to antimethanogenic effects of certain plant species and plant secondary metabolites and on secondary metabolites in these species can be described as a major interest for the interpretation of such results and discuss future research to identify the chemical components responsible for antimethanogenic activity.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MED6921.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10273 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MED/6921 MED/6921 Thèse Bibliothèque principale Thèses Disponible
Titre : evaluation chimique et activite antibacterienne de quelques plantes medicinales d’Algerie Type de document : texte imprimé Auteurs : Foulla Kahlouche-Riachi, Auteur ; Youcef Hamdi Pacha, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 131 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
Sciences VétérinairesTags : Etude phytochimique, activité antibactérienne plantes médicinales diffusion en milieu solide diffusion en milieu liquide
Phytochemical study, antibacterial activity, medicinal plants, diffusion in solid medium, diffusion in liquid medium.
"دراسة النباتية، والنشاط المضاد للبكتيريا، والنباتات الطبية؛ نشرها في المتوسطة الصلبة؛ نشرها في
المتوسط السائ"Index. décimale : 636 Sciences Vétérinaires Résumé : "Medicinal plants are an immense source of bioactive molecules, with many activities. The objective of our work is to achieve a phytochemical and biological study of alcoholic extracts and acetate ethyl phases (AE ) diethyl ether (DE ) and 1 -butanol (1B) of the bark of Pinus pinaster (PPI ), flower buds of Marrubium vulgare ( MUV ) and aerial parts of Artemisia herba alba (AHA) . These three Algerian plants species are used in traditional medicine. The phytochemical screening showed that three plants contain flavonoids, sterols and terpenes, tannins and coumarins. However, only PPI contains alkaloids. Spectral analysis of the methanol extracts reveals the presence of flavonoids such Flavonol, Flavone, Flavanone, Flavanol or anthocyanin.
Studies have been carried out in order to demonstrate the in vitro antibacterial properties of alcoholic extracts and AE, ED and 1B phases of Pinus pinaster bark, flowering tops of Marubium vulgare and the aerial part of Artemesia herba alba obtained after exhaustion with organic solvents of increasing polarity. All the plants proved to be active on one or more bacteria in 25 mg / ml, the effects of the extracts were compared to that of ampicillin, a positive control. By the method of diffusion in solid media (disk), and the diffusion method in liquid medium (MIC), both PPI and MUV extracts showed higher antibacterial activity on Escherichia coli than AHA, the areas of inhibition were 14.33 ± 3.51 and 15.33 ± 2.08 mm respectively . This important activity has been demonstrated in Escherichia coli with an MIC of 0.195mg/ml. The hydroalcoholic extract of AHA showed significant activity compared to positive control (AMPIC ) equal to 19 ± 1.00 with an MIC of 0.097 mg / ml against Pseudomonas aeruginosa. An inhibition diameter of 10 ± 1.00 mm was observed with the hydroalcoholic extract of MUV against Proteus vulgaris with an MIC equal to 0.048mg/ml. The MUV, AHA and PPI extracts were active with inhibition diameters of 16.66 ± 1.53, 13.66 ± 3.21 and 12.33 ± 2.52 respectively against Pseudomonas aeroginosa. Our results also indicate that the phases of plant extracts are little or no active which shows the existence of a synergistic action at the level of molecules present in hydro ethanolic extract. Analysis by thin layer chromatography (TLC) of the three plant extracts showed their high flavonoid, terpene and tannin compounds suggesting the presence of a correlation between the antibacterial activity and the content of the plant."
Diplôme : Doctorat en sciences En ligne : ../theses/veterinaire/KAH6514.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9628 evaluation chimique et activite antibacterienne de quelques plantes medicinales d’Algerie [texte imprimé] / Foulla Kahlouche-Riachi, Auteur ; Youcef Hamdi Pacha, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 131 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
Sciences VétérinairesTags : Etude phytochimique, activité antibactérienne plantes médicinales diffusion en milieu solide diffusion en milieu liquide
Phytochemical study, antibacterial activity, medicinal plants, diffusion in solid medium, diffusion in liquid medium.
"دراسة النباتية، والنشاط المضاد للبكتيريا، والنباتات الطبية؛ نشرها في المتوسطة الصلبة؛ نشرها في
المتوسط السائ"Index. décimale : 636 Sciences Vétérinaires Résumé : "Medicinal plants are an immense source of bioactive molecules, with many activities. The objective of our work is to achieve a phytochemical and biological study of alcoholic extracts and acetate ethyl phases (AE ) diethyl ether (DE ) and 1 -butanol (1B) of the bark of Pinus pinaster (PPI ), flower buds of Marrubium vulgare ( MUV ) and aerial parts of Artemisia herba alba (AHA) . These three Algerian plants species are used in traditional medicine. The phytochemical screening showed that three plants contain flavonoids, sterols and terpenes, tannins and coumarins. However, only PPI contains alkaloids. Spectral analysis of the methanol extracts reveals the presence of flavonoids such Flavonol, Flavone, Flavanone, Flavanol or anthocyanin.
Studies have been carried out in order to demonstrate the in vitro antibacterial properties of alcoholic extracts and AE, ED and 1B phases of Pinus pinaster bark, flowering tops of Marubium vulgare and the aerial part of Artemesia herba alba obtained after exhaustion with organic solvents of increasing polarity. All the plants proved to be active on one or more bacteria in 25 mg / ml, the effects of the extracts were compared to that of ampicillin, a positive control. By the method of diffusion in solid media (disk), and the diffusion method in liquid medium (MIC), both PPI and MUV extracts showed higher antibacterial activity on Escherichia coli than AHA, the areas of inhibition were 14.33 ± 3.51 and 15.33 ± 2.08 mm respectively . This important activity has been demonstrated in Escherichia coli with an MIC of 0.195mg/ml. The hydroalcoholic extract of AHA showed significant activity compared to positive control (AMPIC ) equal to 19 ± 1.00 with an MIC of 0.097 mg / ml against Pseudomonas aeruginosa. An inhibition diameter of 10 ± 1.00 mm was observed with the hydroalcoholic extract of MUV against Proteus vulgaris with an MIC equal to 0.048mg/ml. The MUV, AHA and PPI extracts were active with inhibition diameters of 16.66 ± 1.53, 13.66 ± 3.21 and 12.33 ± 2.52 respectively against Pseudomonas aeroginosa. Our results also indicate that the phases of plant extracts are little or no active which shows the existence of a synergistic action at the level of molecules present in hydro ethanolic extract. Analysis by thin layer chromatography (TLC) of the three plant extracts showed their high flavonoid, terpene and tannin compounds suggesting the presence of a correlation between the antibacterial activity and the content of the plant."
Diplôme : Doctorat en sciences En ligne : ../theses/veterinaire/KAH6514.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9628 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KAH/6514 KAH/6514 Thèse Bibliothèque principale Thèses Disponible
Titre : Effet de diverses plantes médicinales et de leurs huiles essentielles sur la méthanogénèse ruminale chez l’ovin. Type de document : texte imprimé Auteurs : Karima Khenaka, Auteur ; Rabah Arhab, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2011 Importance : 81 f. Format : 31 cm Note générale : Magister
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : méthane plantes médicinales huiles essentielles rumen. Biologie Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/KHE5876.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=5687 Effet de diverses plantes médicinales et de leurs huiles essentielles sur la méthanogénèse ruminale chez l’ovin. [texte imprimé] / Karima Khenaka, Auteur ; Rabah Arhab, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2011 . - 81 f. ; 31 cm.
Magister
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : méthane plantes médicinales huiles essentielles rumen. Biologie Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/KHE5876.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=5687 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KHE/5876 KHE/5876 Thèse Bibliothèque principale Thèses Disponible
Titre : Propriétés antioxydantes et activité inhibitrice de la Xanthine oxydase des extraits de la plante médicinale Ajuga iva (L.) Schreber. Type de document : texte imprimé Auteurs : Moufida Adjadj, Auteur ; Abderrahmane Baghiani, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2009 Importance : 96 f. Format : 30 cm. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Flavonoides Stress oxydatif Antioxydants Plantes médicinales Ajuga iva Effet scavenger DPPH Péroxydationlipidique Béta-caroèe/acide linoléique Index. décimale : 570 Sciences de la vie. Biologie Résumé : This study was conducted to search for XO inhibitors from Ajuga iva shoot extracts
(AISE) traditionally used in folk medicine in Algeria. XO was purified from fresh bovine
milk with yields of ? ? ? ? ? mg / L, protein / flavine ratio of 5.15 and a single major band, on
SDS-PAGE, of approximately 150 KDa, indicating a high purity of the purified enzyme. The
specific activity of the purified enzyme was of 2055.05 nmol / min / mg protein. The analysis
of methanol (CrE), chloroform (ChE) and ethyl acetate (EaE) extracts, showed that the EaE
has significant amount of polyphenols (35.83 ± 0.69 mg EGA / g dry extract), followed by
ChE and CrE (30.58 ± 0.2 and 17.38 ± 0.29 mg EGA / g dry extract, respectively). The ChE
has the highest flavonoids content (9.64 ± 0.33 mg EQ / g dry extract), followed by EaE and
CrE with 7.72 ± 0.19 et 4.92 ± 0.30 mg EQ / g dry extract, respectively. The ChE showed the
highest inhibitory properties on the XO activity (IC50 = 0.134 ± 0.00032 mg / ml) and on the
cytochrome c reduction (IC50 = 0.23 ± 0.0017 mg / ml), followed by EaE with IC50 of XO
inhibitory activity and an IC50 of the inhibition of cyt c reduction of 0.185 ± 0.0019 and 1.046
± 0.0055 mg /ml, respectively. Finally, CrE had the lowest inhibitory effect on XO activity
and on cyt c reduction with 0.367 ± 0.0041 and 3.34 ± 0.018 mg / ml, respectively. The
antioxidant potential was confirmed with the non enzymatic methods, DPPH assay and β-
carotene / linoleic acid bleaching assay. The highest DPPH radical scavenging activity was
observed with EaE (IC50 = 228.01 ± 2.26 µg / ml), followed by CrE and ChE with IC50 of
415.06 ± 0.59 and 466.33 ± 3.20 µg / ml, respectively. The β-carotene / linoleic acid
bleaching assay revealed that AISE have a very important antioxidant activity. Results
showed that ChE has the highest antioxidant activity (95.64 ± 0.15 %), followed by CrE and
EaE with 87.97 ± 0.10 % and 72.30 ± 0.35 %, respectively. These results suggest that these
natural products could be used to treat lot of diseases, where inhibition of XO, free radical
scavenging activity and / or inhibition of lipid peroxidation are necessary.Diplôme : Magistère En ligne : ../theses/biologie/ADJ5396.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1800 Propriétés antioxydantes et activité inhibitrice de la Xanthine oxydase des extraits de la plante médicinale Ajuga iva (L.) Schreber. [texte imprimé] / Moufida Adjadj, Auteur ; Abderrahmane Baghiani, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2009 . - 96 f. ; 30 cm.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Flavonoides Stress oxydatif Antioxydants Plantes médicinales Ajuga iva Effet scavenger DPPH Péroxydationlipidique Béta-caroèe/acide linoléique Index. décimale : 570 Sciences de la vie. Biologie Résumé : This study was conducted to search for XO inhibitors from Ajuga iva shoot extracts
(AISE) traditionally used in folk medicine in Algeria. XO was purified from fresh bovine
milk with yields of ? ? ? ? ? mg / L, protein / flavine ratio of 5.15 and a single major band, on
SDS-PAGE, of approximately 150 KDa, indicating a high purity of the purified enzyme. The
specific activity of the purified enzyme was of 2055.05 nmol / min / mg protein. The analysis
of methanol (CrE), chloroform (ChE) and ethyl acetate (EaE) extracts, showed that the EaE
has significant amount of polyphenols (35.83 ± 0.69 mg EGA / g dry extract), followed by
ChE and CrE (30.58 ± 0.2 and 17.38 ± 0.29 mg EGA / g dry extract, respectively). The ChE
has the highest flavonoids content (9.64 ± 0.33 mg EQ / g dry extract), followed by EaE and
CrE with 7.72 ± 0.19 et 4.92 ± 0.30 mg EQ / g dry extract, respectively. The ChE showed the
highest inhibitory properties on the XO activity (IC50 = 0.134 ± 0.00032 mg / ml) and on the
cytochrome c reduction (IC50 = 0.23 ± 0.0017 mg / ml), followed by EaE with IC50 of XO
inhibitory activity and an IC50 of the inhibition of cyt c reduction of 0.185 ± 0.0019 and 1.046
± 0.0055 mg /ml, respectively. Finally, CrE had the lowest inhibitory effect on XO activity
and on cyt c reduction with 0.367 ± 0.0041 and 3.34 ± 0.018 mg / ml, respectively. The
antioxidant potential was confirmed with the non enzymatic methods, DPPH assay and β-
carotene / linoleic acid bleaching assay. The highest DPPH radical scavenging activity was
observed with EaE (IC50 = 228.01 ± 2.26 µg / ml), followed by CrE and ChE with IC50 of
415.06 ± 0.59 and 466.33 ± 3.20 µg / ml, respectively. The β-carotene / linoleic acid
bleaching assay revealed that AISE have a very important antioxidant activity. Results
showed that ChE has the highest antioxidant activity (95.64 ± 0.15 %), followed by CrE and
EaE with 87.97 ± 0.10 % and 72.30 ± 0.35 %, respectively. These results suggest that these
natural products could be used to treat lot of diseases, where inhibition of XO, free radical
scavenging activity and / or inhibition of lipid peroxidation are necessary.Diplôme : Magistère En ligne : ../theses/biologie/ADJ5396.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1800 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ADJ/5396 ADJ/5396 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude des propriétés antidiabétiques, antioxydantes et antiinflammatoires des extraits hydrosolubles d’Anvillea radiataCoss. & Dur. sur le diabète de type 2 expérimental induit par le régime (high fat) chez la souris C57/BL6J. Type de document : texte imprimé Auteurs : Chouaib Kandouli, Auteur ; Aicha Mechakra (Née Maza), Directeur de thèse ; Sylvia Piertri, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 147 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Anvillea radiata plantes médicinales phénoliques totaux cellules bêtapancréatiques souris C57 / BL6J diabète de type 2 Medicinal plants Total phenolics Pancreatic betacells C57/BL6J mice Type 2 diabetes النباتات الطبية محتوى الفينولات السكري نوع 2 الخلايا β البنكرياسية فئران C57B /6J Index. décimale : 570 Sciences de la vie. Biologie Résumé : Ethnopharmacological relevance: According to Saharian traditional medicine, Anvillea radiata Coss. & Dur. (Asteraceae) has been valued for treating a variety of ailments such as gastro-intestinal, liver and pulmonary diseases, and has gained awareness for its beneficial effect on postprandial hyperglycemia. However, to best of our knowledge, no detailed study of the antidiabetic curative effects of this plant has been conducted yet.
Aim of the study: To determine the hypoglycemic and antidiabetic effect of dietary supplementation with Anvillea radiata extracts on high-fat-diet (HFD)-induced obesity and insulin resistance in C57BL/6 J mice in relation with antioxidant, anti-inflammatory, pancreatic beta-cells and skeletal muscle protection, and digestive enzyme inhibiting properties.
Materials and methods: Six extracts (water soluble and organic) from aerial parts of the plant were analyzed phytochemically (total phenolic and flavonoid content) and screened for in vitro superoxide (by chemiluminescence) and hydroxyl radical (by electron paramagnetic resonance spin-trapping) scavenging, antioxidant (DPPH, TRAP and ORAC assays), xanthine oxidase, metal chelating, α-amylase and α-glucosidase inhibitory property, and protective effects on copper-induced lipoprotein oxidation. Then selected
hydroalcoholic and aqueous extracts were assessed for toxicity in normal human lung fibroblasts and A549 cancer cells using FMCA and MTT assays. Two water-soluble extracts having the best overall properties were assessed for their (i) protective effect at 1−15 µg/mL on metabolic activity of rat insulinoma-derived INS-1 cells exposed to
hyperglycemic medium, and (ii) acute hypoglycemic effect on 16-weeks HFD-induced diabetic mice. Then diabetic mice were administered HFD supplemented by extracts (up to 150 mg/kg/day) for 12 additional weeks using standard diet as control and the antidiabetic drug, metformin (150 mg/kg), as positive control. Then the antidiabetic, anti-inflammatory and antioxidant activity of extracts were determined.
Results: Of the highly efficient polyphenolics-enriched hydroalcoholic and ethyl acetate extracts, the lyophilized aqueous (AQL) and butanol extracts were not toxic in cells (≤ 400 µg/mL) or when given orally in normal mice (≤ 2000 mg/kg), exerted a dosedependent hypoglycemic action in diabetic mice, which was maximal at the dose of 150 mg/kg. Upon administering this dose for 12 weeks, both extracts significantly ameliorated
Résumés body weight control capacity, recovery of plasma glucose and insulin level, reduced oxidative stress in blood, myocardial and skeletal muscles, and improved hyperlipidemic and inflammatory status. Moreover, diabetes-related complications were optimally ameliorated by oral therapy based on halved doses (75 mg/kg) of a mixture of AQL andmetformin.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KAN7266.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10863 Etude des propriétés antidiabétiques, antioxydantes et antiinflammatoires des extraits hydrosolubles d’Anvillea radiataCoss. & Dur. sur le diabète de type 2 expérimental induit par le régime (high fat) chez la souris C57/BL6J. [texte imprimé] / Chouaib Kandouli, Auteur ; Aicha Mechakra (Née Maza), Directeur de thèse ; Sylvia Piertri, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 147 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Anvillea radiata plantes médicinales phénoliques totaux cellules bêtapancréatiques souris C57 / BL6J diabète de type 2 Medicinal plants Total phenolics Pancreatic betacells C57/BL6J mice Type 2 diabetes النباتات الطبية محتوى الفينولات السكري نوع 2 الخلايا β البنكرياسية فئران C57B /6J Index. décimale : 570 Sciences de la vie. Biologie Résumé : Ethnopharmacological relevance: According to Saharian traditional medicine, Anvillea radiata Coss. & Dur. (Asteraceae) has been valued for treating a variety of ailments such as gastro-intestinal, liver and pulmonary diseases, and has gained awareness for its beneficial effect on postprandial hyperglycemia. However, to best of our knowledge, no detailed study of the antidiabetic curative effects of this plant has been conducted yet.
Aim of the study: To determine the hypoglycemic and antidiabetic effect of dietary supplementation with Anvillea radiata extracts on high-fat-diet (HFD)-induced obesity and insulin resistance in C57BL/6 J mice in relation with antioxidant, anti-inflammatory, pancreatic beta-cells and skeletal muscle protection, and digestive enzyme inhibiting properties.
Materials and methods: Six extracts (water soluble and organic) from aerial parts of the plant were analyzed phytochemically (total phenolic and flavonoid content) and screened for in vitro superoxide (by chemiluminescence) and hydroxyl radical (by electron paramagnetic resonance spin-trapping) scavenging, antioxidant (DPPH, TRAP and ORAC assays), xanthine oxidase, metal chelating, α-amylase and α-glucosidase inhibitory property, and protective effects on copper-induced lipoprotein oxidation. Then selected
hydroalcoholic and aqueous extracts were assessed for toxicity in normal human lung fibroblasts and A549 cancer cells using FMCA and MTT assays. Two water-soluble extracts having the best overall properties were assessed for their (i) protective effect at 1−15 µg/mL on metabolic activity of rat insulinoma-derived INS-1 cells exposed to
hyperglycemic medium, and (ii) acute hypoglycemic effect on 16-weeks HFD-induced diabetic mice. Then diabetic mice were administered HFD supplemented by extracts (up to 150 mg/kg/day) for 12 additional weeks using standard diet as control and the antidiabetic drug, metformin (150 mg/kg), as positive control. Then the antidiabetic, anti-inflammatory and antioxidant activity of extracts were determined.
Results: Of the highly efficient polyphenolics-enriched hydroalcoholic and ethyl acetate extracts, the lyophilized aqueous (AQL) and butanol extracts were not toxic in cells (≤ 400 µg/mL) or when given orally in normal mice (≤ 2000 mg/kg), exerted a dosedependent hypoglycemic action in diabetic mice, which was maximal at the dose of 150 mg/kg. Upon administering this dose for 12 weeks, both extracts significantly ameliorated
Résumés body weight control capacity, recovery of plasma glucose and insulin level, reduced oxidative stress in blood, myocardial and skeletal muscles, and improved hyperlipidemic and inflammatory status. Moreover, diabetes-related complications were optimally ameliorated by oral therapy based on halved doses (75 mg/kg) of a mixture of AQL andmetformin.
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Code-barres Cote Support Localisation Section Disponibilité KAN/7266 KAN/7266 Thèse Bibliothèque principale Thèses Disponible
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