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Partager le résultat de cette recherche Interroger des sources externesPropriétés antioxydantes et activité inhibitrice de la Xanthine oxydase des extraits de la plante médicinale Ajuga iva (L.) Schreber. / Moufida Adjadj
Titre : Propriétés antioxydantes et activité inhibitrice de la Xanthine oxydase des extraits de la plante médicinale Ajuga iva (L.) Schreber. Type de document : texte imprimé Auteurs : Moufida Adjadj, Auteur ; Abderrahmane Baghiani, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2009 Importance : 96 f. Format : 30 cm. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Flavonoides Stress oxydatif Antioxydants Plantes médicinales Ajuga iva Effet scavenger DPPH Péroxydationlipidique Béta-caroèe/acide linoléique Index. décimale : 570 Sciences de la vie. Biologie Résumé : This study was conducted to search for XO inhibitors from Ajuga iva shoot extracts
(AISE) traditionally used in folk medicine in Algeria. XO was purified from fresh bovine
milk with yields of ? ? ? ? ? mg / L, protein / flavine ratio of 5.15 and a single major band, on
SDS-PAGE, of approximately 150 KDa, indicating a high purity of the purified enzyme. The
specific activity of the purified enzyme was of 2055.05 nmol / min / mg protein. The analysis
of methanol (CrE), chloroform (ChE) and ethyl acetate (EaE) extracts, showed that the EaE
has significant amount of polyphenols (35.83 ± 0.69 mg EGA / g dry extract), followed by
ChE and CrE (30.58 ± 0.2 and 17.38 ± 0.29 mg EGA / g dry extract, respectively). The ChE
has the highest flavonoids content (9.64 ± 0.33 mg EQ / g dry extract), followed by EaE and
CrE with 7.72 ± 0.19 et 4.92 ± 0.30 mg EQ / g dry extract, respectively. The ChE showed the
highest inhibitory properties on the XO activity (IC50 = 0.134 ± 0.00032 mg / ml) and on the
cytochrome c reduction (IC50 = 0.23 ± 0.0017 mg / ml), followed by EaE with IC50 of XO
inhibitory activity and an IC50 of the inhibition of cyt c reduction of 0.185 ± 0.0019 and 1.046
± 0.0055 mg /ml, respectively. Finally, CrE had the lowest inhibitory effect on XO activity
and on cyt c reduction with 0.367 ± 0.0041 and 3.34 ± 0.018 mg / ml, respectively. The
antioxidant potential was confirmed with the non enzymatic methods, DPPH assay and β-
carotene / linoleic acid bleaching assay. The highest DPPH radical scavenging activity was
observed with EaE (IC50 = 228.01 ± 2.26 µg / ml), followed by CrE and ChE with IC50 of
415.06 ± 0.59 and 466.33 ± 3.20 µg / ml, respectively. The β-carotene / linoleic acid
bleaching assay revealed that AISE have a very important antioxidant activity. Results
showed that ChE has the highest antioxidant activity (95.64 ± 0.15 %), followed by CrE and
EaE with 87.97 ± 0.10 % and 72.30 ± 0.35 %, respectively. These results suggest that these
natural products could be used to treat lot of diseases, where inhibition of XO, free radical
scavenging activity and / or inhibition of lipid peroxidation are necessary.Diplôme : Magistère En ligne : ../theses/biologie/ADJ5396.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1800 Propriétés antioxydantes et activité inhibitrice de la Xanthine oxydase des extraits de la plante médicinale Ajuga iva (L.) Schreber. [texte imprimé] / Moufida Adjadj, Auteur ; Abderrahmane Baghiani, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2009 . - 96 f. ; 30 cm.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Flavonoides Stress oxydatif Antioxydants Plantes médicinales Ajuga iva Effet scavenger DPPH Péroxydationlipidique Béta-caroèe/acide linoléique Index. décimale : 570 Sciences de la vie. Biologie Résumé : This study was conducted to search for XO inhibitors from Ajuga iva shoot extracts
(AISE) traditionally used in folk medicine in Algeria. XO was purified from fresh bovine
milk with yields of ? ? ? ? ? mg / L, protein / flavine ratio of 5.15 and a single major band, on
SDS-PAGE, of approximately 150 KDa, indicating a high purity of the purified enzyme. The
specific activity of the purified enzyme was of 2055.05 nmol / min / mg protein. The analysis
of methanol (CrE), chloroform (ChE) and ethyl acetate (EaE) extracts, showed that the EaE
has significant amount of polyphenols (35.83 ± 0.69 mg EGA / g dry extract), followed by
ChE and CrE (30.58 ± 0.2 and 17.38 ± 0.29 mg EGA / g dry extract, respectively). The ChE
has the highest flavonoids content (9.64 ± 0.33 mg EQ / g dry extract), followed by EaE and
CrE with 7.72 ± 0.19 et 4.92 ± 0.30 mg EQ / g dry extract, respectively. The ChE showed the
highest inhibitory properties on the XO activity (IC50 = 0.134 ± 0.00032 mg / ml) and on the
cytochrome c reduction (IC50 = 0.23 ± 0.0017 mg / ml), followed by EaE with IC50 of XO
inhibitory activity and an IC50 of the inhibition of cyt c reduction of 0.185 ± 0.0019 and 1.046
± 0.0055 mg /ml, respectively. Finally, CrE had the lowest inhibitory effect on XO activity
and on cyt c reduction with 0.367 ± 0.0041 and 3.34 ± 0.018 mg / ml, respectively. The
antioxidant potential was confirmed with the non enzymatic methods, DPPH assay and β-
carotene / linoleic acid bleaching assay. The highest DPPH radical scavenging activity was
observed with EaE (IC50 = 228.01 ± 2.26 µg / ml), followed by CrE and ChE with IC50 of
415.06 ± 0.59 and 466.33 ± 3.20 µg / ml, respectively. The β-carotene / linoleic acid
bleaching assay revealed that AISE have a very important antioxidant activity. Results
showed that ChE has the highest antioxidant activity (95.64 ± 0.15 %), followed by CrE and
EaE with 87.97 ± 0.10 % and 72.30 ± 0.35 %, respectively. These results suggest that these
natural products could be used to treat lot of diseases, where inhibition of XO, free radical
scavenging activity and / or inhibition of lipid peroxidation are necessary.Diplôme : Magistère En ligne : ../theses/biologie/ADJ5396.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1800 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ADJ/5396 ADJ/5396 Thèse Bibliothèque principale Thèses Disponible
Titre : L’effet protecteur des antioxydants naturels dans l’intoxication du mercure chez le rat Albinos Wistar : (Aspects biochimiques, immunologiques et histologiques). Type de document : texte imprimé Auteurs : Ahlem Bahi, Auteur ; Youcef Necib, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2015 Importance : 158 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biochimie appliquee Mercure Huile d’argan Sélénium Stress oxydatif Argania Spinosa antioxydants Mercury Argan Oil Selenium Oxidative Stress antioxidants السلينيوم الإجهاد التاكسدي زيت الأركان الزئبق Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present study aimed to evaluate the protective effect of argan oil and selenium against oxidative stress induced by mercury. For this reason, a phytochemical study of antioxidant tested in vitro was performed on argan oil to estimate its expected effect. The results showed the presence of phenolic compounds in a very high concentration. The evaluation of power scavenger argan oil is more active with IC50 = 13.33 ± 1.3, and in vivo on Albino Wistar rats were treated with mercury alone or combined with argan oil and selenium, for a period of three weeks, the blood is then withdrawn and the rats are sacrificed the various parameters are determined. From the analysis of our results, we observed a significant reduction in weight gain during treatment with mercury alone or combined with argan oil and selenium, but no clear change in the relative weight of liver, kidney or spleen was obtained. The results also showed significant changes in biochemical parameters, immunological and haematological. Characterized primarily by a significant increase in serum glucose, urea, creatinine, uric acid, total and direct bilirubin, total lipids, cholesterol, triglyceride, conjugated diene and the enzymatic activity of AST, ALT, LDH and PAL .dropoff window Whereas serum concentrations of albumin and total protein were reduced in treated rats compared to mercury control animals. Results also show immunological and haematological toxicity especially among the treated groups the pure mercury, an increase in serum IL-1, IL-6 and TNF and elevated white blood cell count, decreased red blood cell levels, hemoglobin concentration the percentage of hematocrit and phagocytic activity during treatment with mercury. Thus the treatment of the rats with the mercury causes a reduction in the plasma antioxidant capacity characterized by decrease in the serum concentration of vitamin E and C and rate of DPPH and thiols. We also observed that the results obtained highlight the detoxifier potential expressed by the decrease in erythrocyte glutathione levels, liver and kidney, metallothionein, the enzymatic activity of the antioxidant system SOD, GPx, GST and catalase and increased in lipid peroxidation expressed by the high levels of plasma MDA, erythrocyte, liver and kidney. Also the results clearly showed substantive alterations of tissue studies in comparison with the control, with hepatocyte cytolysis, hepatic necrosis and tubular degeneration with necrosis of the kidneys. Furthermore supplementation argan oil and selenium changed the toxic effects of mercury and decreased histological alterations and showed that both antioxidants are effective against oxidative stress induced by mercury
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BAH6696.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9928 L’effet protecteur des antioxydants naturels dans l’intoxication du mercure chez le rat Albinos Wistar : (Aspects biochimiques, immunologiques et histologiques). [texte imprimé] / Ahlem Bahi, Auteur ; Youcef Necib, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2015 . - 158 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biochimie appliquee Mercure Huile d’argan Sélénium Stress oxydatif Argania Spinosa antioxydants Mercury Argan Oil Selenium Oxidative Stress antioxidants السلينيوم الإجهاد التاكسدي زيت الأركان الزئبق Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present study aimed to evaluate the protective effect of argan oil and selenium against oxidative stress induced by mercury. For this reason, a phytochemical study of antioxidant tested in vitro was performed on argan oil to estimate its expected effect. The results showed the presence of phenolic compounds in a very high concentration. The evaluation of power scavenger argan oil is more active with IC50 = 13.33 ± 1.3, and in vivo on Albino Wistar rats were treated with mercury alone or combined with argan oil and selenium, for a period of three weeks, the blood is then withdrawn and the rats are sacrificed the various parameters are determined. From the analysis of our results, we observed a significant reduction in weight gain during treatment with mercury alone or combined with argan oil and selenium, but no clear change in the relative weight of liver, kidney or spleen was obtained. The results also showed significant changes in biochemical parameters, immunological and haematological. Characterized primarily by a significant increase in serum glucose, urea, creatinine, uric acid, total and direct bilirubin, total lipids, cholesterol, triglyceride, conjugated diene and the enzymatic activity of AST, ALT, LDH and PAL .dropoff window Whereas serum concentrations of albumin and total protein were reduced in treated rats compared to mercury control animals. Results also show immunological and haematological toxicity especially among the treated groups the pure mercury, an increase in serum IL-1, IL-6 and TNF and elevated white blood cell count, decreased red blood cell levels, hemoglobin concentration the percentage of hematocrit and phagocytic activity during treatment with mercury. Thus the treatment of the rats with the mercury causes a reduction in the plasma antioxidant capacity characterized by decrease in the serum concentration of vitamin E and C and rate of DPPH and thiols. We also observed that the results obtained highlight the detoxifier potential expressed by the decrease in erythrocyte glutathione levels, liver and kidney, metallothionein, the enzymatic activity of the antioxidant system SOD, GPx, GST and catalase and increased in lipid peroxidation expressed by the high levels of plasma MDA, erythrocyte, liver and kidney. Also the results clearly showed substantive alterations of tissue studies in comparison with the control, with hepatocyte cytolysis, hepatic necrosis and tubular degeneration with necrosis of the kidneys. Furthermore supplementation argan oil and selenium changed the toxic effects of mercury and decreased histological alterations and showed that both antioxidants are effective against oxidative stress induced by mercury
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BAH6696.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9928 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BAH/6696 BAH/6696 Thèse Bibliothèque principale Thèses Disponible
Titre : Effet antioxydant de l’huile essentielle de la plante Guezzah (Pituranthos chloranthus) incorporée dans des shortenings produits au niveau de CEVITAL SPA. Type de document : texte imprimé Auteurs : Rima Sabouni, Auteur ; H. Namoune, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 74 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Pituranthos chloranthus shortening oxydation antioxydants huile essentielle Essential oil الأكسدة مضادات الأكسدة الزيت الأساسي Index. décimale : 640 Industrie Alimentaire Résumé : Through the present study on the assessment of the antioxidant activity of Pituranthos chloranthus’ essential oil, endemic plant of the Algerian Sahara high plateau and its incorporation in a shortening formulation produced in at a laboratory scale in a margarinery of CEVITAL Food Company, it is revealed that:
Aerial part of Pituranthos chloranthus showed a moisture of (11,8 ± 0,097) % and an essential oil’s extraction yield, extracted by hydrodistillation, is near (0,86 ± 0,09) % and an optimization of time (t = 250min) for a dry plant mass of de (m = 80 g).
Antioxidant activity of the present plant material, determined by three tests: reducing power,DPPH and
-carotene bleaching test against a standard (α-Tocopherol or Vitamine E). The reducing power is of (0.094 ± 0.018) g TE/g EO, IC50 of (0,024 ± 0,002) mg/ml and a protective effect against
-carotene oxidation.
Physico-chemical characteristics of the shortening produced including: acidity, peroxide index and melting point are in a good agreement with the internal company standards.
Assessment of oxidative stability is carried out by Rancimat and Schaal tests. Rancimat showed that shortening incorporated with 75ppm gave the best resistance to oxidation. This is also consistent with Shaal test, with a peroxide index of PI = 0, 28 meq g O2/1000g, carried out in 35 days.
Thus, Pituranthos chloranthus essential oil may be of a technological interest to serve like preservatives in fats and oils, especially in food companies.
Diplôme : Magistère En ligne : ../theses/agronomie/SAB6692.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9931 Effet antioxydant de l’huile essentielle de la plante Guezzah (Pituranthos chloranthus) incorporée dans des shortenings produits au niveau de CEVITAL SPA. [texte imprimé] / Rima Sabouni, Auteur ; H. Namoune, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 74 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Pituranthos chloranthus shortening oxydation antioxydants huile essentielle Essential oil الأكسدة مضادات الأكسدة الزيت الأساسي Index. décimale : 640 Industrie Alimentaire Résumé : Through the present study on the assessment of the antioxidant activity of Pituranthos chloranthus’ essential oil, endemic plant of the Algerian Sahara high plateau and its incorporation in a shortening formulation produced in at a laboratory scale in a margarinery of CEVITAL Food Company, it is revealed that:
Aerial part of Pituranthos chloranthus showed a moisture of (11,8 ± 0,097) % and an essential oil’s extraction yield, extracted by hydrodistillation, is near (0,86 ± 0,09) % and an optimization of time (t = 250min) for a dry plant mass of de (m = 80 g).
Antioxidant activity of the present plant material, determined by three tests: reducing power,DPPH and
-carotene bleaching test against a standard (α-Tocopherol or Vitamine E). The reducing power is of (0.094 ± 0.018) g TE/g EO, IC50 of (0,024 ± 0,002) mg/ml and a protective effect against
-carotene oxidation.
Physico-chemical characteristics of the shortening produced including: acidity, peroxide index and melting point are in a good agreement with the internal company standards.
Assessment of oxidative stability is carried out by Rancimat and Schaal tests. Rancimat showed that shortening incorporated with 75ppm gave the best resistance to oxidation. This is also consistent with Shaal test, with a peroxide index of PI = 0, 28 meq g O2/1000g, carried out in 35 days.
Thus, Pituranthos chloranthus essential oil may be of a technological interest to serve like preservatives in fats and oils, especially in food companies.
Diplôme : Magistère En ligne : ../theses/agronomie/SAB6692.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9931 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité SAB/6692 SAB/6692 Thèse Bibliothèque principale Thèses Disponible
Titre : Effet de l'extrait méthanolique de zygophyllum cornutum coss contre le strss oxydant associé au diabLte sucré et les organes en relation Type de document : texte imprimé Auteurs : Univ. de Constantine, Éditeur scientifique Année de publication : 2009 Importance : 104 f. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : DiabLte Antioxydants Zygophyllum cornutum coss Stress oxydative Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/BOU5510.pdf Permalink : index.php?lvl=notice_display&id=1811 Effet de l'extrait méthanolique de zygophyllum cornutum coss contre le strss oxydant associé au diabLte sucré et les organes en relation [texte imprimé] / Univ. de Constantine, Éditeur scientifique . - 2009 . - 104 f.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : DiabLte Antioxydants Zygophyllum cornutum coss Stress oxydative Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/BOU5510.pdf Permalink : index.php?lvl=notice_display&id=1811 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/5510 BOU/5510 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude Analytique et Biologique des Flavonoïdes Naturels. Type de document : texte imprimé Auteurs : Souad Akroum, Auteur ; Korrichi Lalaoui, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2011 Importance : 116 f. Format : 31 cm Note générale : Doctorat en sciences
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Plantes extraits flavonoїdes antimicrobiens antioxydants cytotoxiques toxicité aigue tannins condensés moisissures tannase. Biologie Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AKR5942.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=5771 Etude Analytique et Biologique des Flavonoïdes Naturels. [texte imprimé] / Souad Akroum, Auteur ; Korrichi Lalaoui, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2011 . - 116 f. ; 31 cm.
Doctorat en sciences
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Plantes extraits flavonoїdes antimicrobiens antioxydants cytotoxiques toxicité aigue tannins condensés moisissures tannase. Biologie Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AKR5942.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=5771 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AKR/5942 AKR/5942 Thèse Bibliothèque principale Thèses Disponible Permalink
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