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Partager le résultat de cette recherche Interroger des sources externesRecherche et évaluation des activités biologiques de trois extraits d’Helix aspersa (aqueux, hydro alcoolique et organique) / Romeila Mebirouk
Titre : Recherche et évaluation des activités biologiques de trois extraits d’Helix aspersa (aqueux, hydro alcoolique et organique) : Activités anti-inflammatoire, anti tumorale et anti-angiogénique Type de document : texte imprimé Auteurs : Romeila Mebirouk, Auteur ; Dalila Naimi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 147 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Helix aspersa extracts biomolecules inflammatory reaction tumor cells protease tumor invasion extraits biomolécules réaction inflammatoire cellules tumorales protéase invasion tumoral ھیلیكس اسبرسا مستخلصات الجزیئات الطبیعیة| تفاعل الالتھابي خلایا سرطانیة بروتیاز غزو الورمي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Although the field of biotechnology has developed extraordinarily in recent years, therapies remain ineffective and can cause side effects in patients, which is why the laboratories have turned to the search for new natural products. Helix aspersa is a terrestrial mollusk that is widespread in Mediterranean countries and is part of the food habitude. It is an important source of protein and mineral salts and has several therapeutic effects known for centuries.
One of the main prospects of our study is the evaluation of the anti-inflammatory, anti-tumor and anti-angiogenic effects. Three extracts were prepared using solvents with different polarity: an aqueous extract, an hydroalcoholic extract and an organic extract. Then, a model of acute pulmonary inflammation was developed to evaluate in vivo the anti-inflammatory activity of extracts by measuring total white blood cells and white blood cells in bronchoalveolar fluid, myeloperoxydase activity and analyzing the histology of pulmonary tissue. Analysis of the results showed that the extracts had anti-inflammatory activity by decreasing the total leukocyte count in blood and in bronchoalveolar fluid, protecting the pulmonary tissue by modulating neutrophil activity and decreasing protein concentration in bronchoalveolar fluid. The antiproliferative activity of the extracts was evaluated by trypan blue and the MTT test on several cell lines: MK2, A549, HUH7, K562, HUT-78 and SeAx.
The chromatin condensation and nuclear fragmentation were evaluated by the Hoechst test in the HUT-78 and SeAx tumor cell line stimulated by the aqueous extract. The aqueous and hydroalcoholic extracts had antiproliferative activity against the HUT-78 and SeAx lines. The aqueous extract induced apoptosis in HUT-78 cells. The hydroalcoholic and organic extracts had an antiproliferative activity on the HUH7 line. The three extracts were not toxic on the MK2 normal cell line. The antiangiogenic effect was evaluated by measuring the expression of MMP-9 in the two HUT-78 and SeAx lines stimulated in the presence of the aqueous and hydroalcoholic extracts using was the zymography method. The aqueous and hydroalcoholic
extracts had no effect on the production of MMP-9. At the end, we extracted the soluble proteins from meat lyophilized and we fractionated the extracts by anions exchange chromatography. The proteolytic activity using synthetic substrates and protease inhibitors as well as the antiproliferative activity of the different fractions obtained were measured. One of the fractions contains proteases and has antiproliferative activity against the K562 liquid line.
Nevertheless, other contained no enzymes and had a toxic effect on the matrix tumor lines A549 and HUH7. It was concluded that the aqueous, hydroalcoholic and organic extracts of Helix aspersa had an anti-inflammatory and an anti-tumor effects, but did not have an antiangiogenic effect (on the expression of MMP-9)
Diplôme : Doctorat En ligne : ../theses/biologie/MEB7013.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10392 Recherche et évaluation des activités biologiques de trois extraits d’Helix aspersa (aqueux, hydro alcoolique et organique) : Activités anti-inflammatoire, anti tumorale et anti-angiogénique [texte imprimé] / Romeila Mebirouk, Auteur ; Dalila Naimi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 147 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Helix aspersa extracts biomolecules inflammatory reaction tumor cells protease tumor invasion extraits biomolécules réaction inflammatoire cellules tumorales protéase invasion tumoral ھیلیكس اسبرسا مستخلصات الجزیئات الطبیعیة| تفاعل الالتھابي خلایا سرطانیة بروتیاز غزو الورمي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Although the field of biotechnology has developed extraordinarily in recent years, therapies remain ineffective and can cause side effects in patients, which is why the laboratories have turned to the search for new natural products. Helix aspersa is a terrestrial mollusk that is widespread in Mediterranean countries and is part of the food habitude. It is an important source of protein and mineral salts and has several therapeutic effects known for centuries.
One of the main prospects of our study is the evaluation of the anti-inflammatory, anti-tumor and anti-angiogenic effects. Three extracts were prepared using solvents with different polarity: an aqueous extract, an hydroalcoholic extract and an organic extract. Then, a model of acute pulmonary inflammation was developed to evaluate in vivo the anti-inflammatory activity of extracts by measuring total white blood cells and white blood cells in bronchoalveolar fluid, myeloperoxydase activity and analyzing the histology of pulmonary tissue. Analysis of the results showed that the extracts had anti-inflammatory activity by decreasing the total leukocyte count in blood and in bronchoalveolar fluid, protecting the pulmonary tissue by modulating neutrophil activity and decreasing protein concentration in bronchoalveolar fluid. The antiproliferative activity of the extracts was evaluated by trypan blue and the MTT test on several cell lines: MK2, A549, HUH7, K562, HUT-78 and SeAx.
The chromatin condensation and nuclear fragmentation were evaluated by the Hoechst test in the HUT-78 and SeAx tumor cell line stimulated by the aqueous extract. The aqueous and hydroalcoholic extracts had antiproliferative activity against the HUT-78 and SeAx lines. The aqueous extract induced apoptosis in HUT-78 cells. The hydroalcoholic and organic extracts had an antiproliferative activity on the HUH7 line. The three extracts were not toxic on the MK2 normal cell line. The antiangiogenic effect was evaluated by measuring the expression of MMP-9 in the two HUT-78 and SeAx lines stimulated in the presence of the aqueous and hydroalcoholic extracts using was the zymography method. The aqueous and hydroalcoholic
extracts had no effect on the production of MMP-9. At the end, we extracted the soluble proteins from meat lyophilized and we fractionated the extracts by anions exchange chromatography. The proteolytic activity using synthetic substrates and protease inhibitors as well as the antiproliferative activity of the different fractions obtained were measured. One of the fractions contains proteases and has antiproliferative activity against the K562 liquid line.
Nevertheless, other contained no enzymes and had a toxic effect on the matrix tumor lines A549 and HUH7. It was concluded that the aqueous, hydroalcoholic and organic extracts of Helix aspersa had an anti-inflammatory and an anti-tumor effects, but did not have an antiangiogenic effect (on the expression of MMP-9)
Diplôme : Doctorat En ligne : ../theses/biologie/MEB7013.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10392 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MEB/7013 MEB/7013 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude Analytique et Biologique des Flavonoïdes Naturels. Type de document : texte imprimé Auteurs : Souad Akroum, Auteur ; Korrichi Lalaoui, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2011 Importance : 116 f. Format : 31 cm Note générale : Doctorat en sciences
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Plantes extraits flavonoїdes antimicrobiens antioxydants cytotoxiques toxicité aigue tannins condensés moisissures tannase. Biologie Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AKR5942.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=5771 Etude Analytique et Biologique des Flavonoïdes Naturels. [texte imprimé] / Souad Akroum, Auteur ; Korrichi Lalaoui, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2011 . - 116 f. ; 31 cm.
Doctorat en sciences
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Plantes extraits flavonoїdes antimicrobiens antioxydants cytotoxiques toxicité aigue tannins condensés moisissures tannase. Biologie Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AKR5942.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=5771 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AKR/5942 AKR/5942 Thèse Bibliothèque principale Thèses Disponible
Titre : Evaluation du potentiel nutritif et antiméthanogénique des plantes médicinales (Asteraceae) par des procédés phytochimiques, biologiques et moléculaires. Type de document : texte imprimé Auteurs : Serine Amokrane, Auteur ; Rabah Arhab, Auteur Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 218 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Microbiologie:Écologie Microbienne Asteraceae C.nobile C.segetum C.pulata extraits valeur nutritive méthanogénése qPCR Archaea Protozoaires extracts nutritive value methanogenesis Protozoa التقويم الغذائي C . Nobile C . segeturn C . pulat til الوقت الحقيقي PCR البكتيريا المنتجة للميثان البر وتوزوا العائلة المركبة عملية انتاج الميثان Index. décimale : 570 Sciences de la vie. Biologie Résumé : The objective of the present work is focused on the evaluation of three Asteraceae (C. nobile, C.pulata and C. segetum) from a nutritional point of view via chemical processes (forage analysis), phytochemicals (phenolic and terpenic content) and biological (fermentability in vitro by the ruminal microbiota of cattle). The environmental aspect consisted in determining the antimethanogenic effect of these same plants as well as their respective extracts. This axis is consolidated by molecular analyzes (real-time PCR) of two microbial communities directly involved in ruminal methanogenesis (Archaea methanogens and protozoa). The practical purpose of the combination of these two approaches is shared between the formulation of dietary diets based on herbs and / or their extracts in order to provide food alternatives to local livestock, on the one hand and to control and reduce atmospheric emissions of enteric methane by ruminants, on the other hand. The chemical and phytochemical analyzes make it possible to classify C.nobile and C.pulata as substrates of good nutritional quality. This is explained by their appreciable content in total walls, their energy and protein richness and the moderate presence of antinutritional substances. Nevertheless, the high bioactive content and low protein content noted for C. segetum suggests that this plant could be valued as a natural source of bioactive substances and poorly recommended as a livestock feed. The results of the evaluation of the in vitro fermentability of the substrates studied, in terms of digestibility, in vitro gas production and fermentative profile (volatile fatty acids), are in perfect agreement with those of previous analyzes. In addition, their fermental profile indicates globally that their content of secondary substances does not influence the activity of the ruminal microbiota. Indeed, the tannoid and flavonoic fractions do not affect the different fermentation parameters after 24 hours of fermentation. The environmental aspect of the study presented by the determination of the antimethanogenic effect of plants and their extracts reveals that the fermentation of centaury increases the production of enteric methane in vitro. Noble chamomile and chrysanthemum have a tangible antimethanogenic potential with significant reduction rates (-24 and -27%, respectively). However, the antimethanogenic effect is increased (-39 and -50%, respectively) in the presence of their respective extracts. The approach of molecular biology has allowed us to have a global and rapid look at the main microbial communities directly involved in the process of ruminal methanogenesis. The results reveal the absence of a relationship between the biomass of Archaea methanogens and the production of methane in the rumen, and their existence with the protozoan community. The symbiotic relationship between these two communities could justify this finding Overall, it appears that the inclusion of C.nobile in the feeding of ruminants as basic forage is strongly recommended. C.pulata is recommended as an energy supplement, which makes it possible to propose them as good condidates for a possible exploitation in the animal feed and a probable reorganization of the courses in the steppic zones. The notable effect of the extracts studied on the reduction of methane production and especially the absence of effects on fermentative parameters (AGV and ammonia) make it possible to classify them as food additives to fight against the famous greenhouse effect.
Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AMO7490.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11322 Evaluation du potentiel nutritif et antiméthanogénique des plantes médicinales (Asteraceae) par des procédés phytochimiques, biologiques et moléculaires. [texte imprimé] / Serine Amokrane, Auteur ; Rabah Arhab, Auteur . - جامعة الإخوة منتوري قسنطينة, 2019 . - 218 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Microbiologie:Écologie Microbienne Asteraceae C.nobile C.segetum C.pulata extraits valeur nutritive méthanogénése qPCR Archaea Protozoaires extracts nutritive value methanogenesis Protozoa التقويم الغذائي C . Nobile C . segeturn C . pulat til الوقت الحقيقي PCR البكتيريا المنتجة للميثان البر وتوزوا العائلة المركبة عملية انتاج الميثان Index. décimale : 570 Sciences de la vie. Biologie Résumé : The objective of the present work is focused on the evaluation of three Asteraceae (C. nobile, C.pulata and C. segetum) from a nutritional point of view via chemical processes (forage analysis), phytochemicals (phenolic and terpenic content) and biological (fermentability in vitro by the ruminal microbiota of cattle). The environmental aspect consisted in determining the antimethanogenic effect of these same plants as well as their respective extracts. This axis is consolidated by molecular analyzes (real-time PCR) of two microbial communities directly involved in ruminal methanogenesis (Archaea methanogens and protozoa). The practical purpose of the combination of these two approaches is shared between the formulation of dietary diets based on herbs and / or their extracts in order to provide food alternatives to local livestock, on the one hand and to control and reduce atmospheric emissions of enteric methane by ruminants, on the other hand. The chemical and phytochemical analyzes make it possible to classify C.nobile and C.pulata as substrates of good nutritional quality. This is explained by their appreciable content in total walls, their energy and protein richness and the moderate presence of antinutritional substances. Nevertheless, the high bioactive content and low protein content noted for C. segetum suggests that this plant could be valued as a natural source of bioactive substances and poorly recommended as a livestock feed. The results of the evaluation of the in vitro fermentability of the substrates studied, in terms of digestibility, in vitro gas production and fermentative profile (volatile fatty acids), are in perfect agreement with those of previous analyzes. In addition, their fermental profile indicates globally that their content of secondary substances does not influence the activity of the ruminal microbiota. Indeed, the tannoid and flavonoic fractions do not affect the different fermentation parameters after 24 hours of fermentation. The environmental aspect of the study presented by the determination of the antimethanogenic effect of plants and their extracts reveals that the fermentation of centaury increases the production of enteric methane in vitro. Noble chamomile and chrysanthemum have a tangible antimethanogenic potential with significant reduction rates (-24 and -27%, respectively). However, the antimethanogenic effect is increased (-39 and -50%, respectively) in the presence of their respective extracts. The approach of molecular biology has allowed us to have a global and rapid look at the main microbial communities directly involved in the process of ruminal methanogenesis. The results reveal the absence of a relationship between the biomass of Archaea methanogens and the production of methane in the rumen, and their existence with the protozoan community. The symbiotic relationship between these two communities could justify this finding Overall, it appears that the inclusion of C.nobile in the feeding of ruminants as basic forage is strongly recommended. C.pulata is recommended as an energy supplement, which makes it possible to propose them as good condidates for a possible exploitation in the animal feed and a probable reorganization of the courses in the steppic zones. The notable effect of the extracts studied on the reduction of methane production and especially the absence of effects on fermentative parameters (AGV and ammonia) make it possible to classify them as food additives to fight against the famous greenhouse effect.
Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AMO7490.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11322 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AMO/7490 AMO/7490 Thèse Bibliothèque principale Thèses Disponible
